ارزیابی اثر افزودن عصاره الکلی گیاهان دارویی آویشن شیرازی و سرخارگل به جیره بر عملکرد، خصوصیات لاشه، فراسنجه‌های بیوشیمیایی خون و وضعیت ایمنی جوجه‌های گوشتی

نوع مقاله : علمی پژوهشی- تغذیه طیور

نویسندگان

دانشگاه گنبد کاووس

چکیده

هدف از این پژوهش، بررسی اثر افزودن عصاره الکلی گیاهان دارویی سرخارگل و آویشن به جیره بر عملکرد، صفات لاشه و همچنین فراسنجه‌های بیوشیمیایی خون و وضعیت ایمنی جوجه‌های گوشتی بود. تعداد 160 قطعه جوجه یک روزه گوشتی سویه راس- 308 (نر و ماده به تعداد مساوی) د قالب طرح کاملاً تصادفی در چهار تیمار با چهار تکرار و هر تکرار ده قطعه، تقسیم شده و پرورش یافتند. طول مدت تحقیق، 42 روز بود که به سه دوره پرورشی آغازین (10-1 روزگی)، رشد (23-11 روزگی) و پایانی (42-24 روزگی) تقسیم شد که تیمارهای آزمایشی از ابتدای دوره اعمال شدند. تیمارهای آزمایشی شامل 1- شاهد (جیره پایه(، 2- افزودن 2/0 درصد عصاره آویشن به جیره پایه، 3- افزودن 2/0 درصد عصاره سرخارگل به جیره پایه و 4- افزودن مخلوط 2/0 درصد عصاره آویشن و 2/0 درصد عصاره سرخارگل به جیره پایه بودند که از روز اول به جوجه‌ها تغذیه شدند. خوراک مصرفی، وزن زنده و ضریب تبدیل غذایی جوجه‌ها به صورت هفتگی ثبت شد. در پایان دوره پرورش، یک پرنده نر از هر تکرار آزمایشی جهت خون‌گیری و تجزیه لاشه کشتار شدند. نتایج نشان داد که اثر تیمارها بر مصرف خوراک و افزایش وزن زنده جوجه‌ها در کل دوره معنی‌دار نبود. ضریب تبدیل خوراک جوجه‌ها به طور معنی‌دار تحت­ تأثیر تیمارهای آزمایشی قرار گرفت. به طوری که بهترین آن در گروه شاهد و بدترین آن در گروه سرخارگل بود. ویژگی‌های لاشه و وزن اندام‌های داخلی جوجه‌ها تحت ­تأثیر تیمارها قرار نگرفت. اثر تیمارهای آزمایشی بر عیار آنتی‌بادی علیه بیماری­های نیوکاسل و آنفولانزا در جوجه‌ها معنی‌دار بود. کمترین آنتی‌بادی علیه نیوکاسل و بیشترین آنتی‌بادی علیه آنفولانزا در گروه آویشن مشاهده شد. افزودن عصاره­های آویشن و سرخارگل به جیره باعث افزایش وزن در وزن غده بورس فابریسیوس جوجه‌ها نسبت به شاهد شد ولی بر وزن طحال تأثیری نداشت. افزودن عصاره­های آویشن و سرخارگل باعث کاهش چشم‌گیر غلظت تری‌گلیسریدها و VLDL خون جوجه‌ها شد. تیمارهای آزمایشی تأثیر معنی‌دار بر تراکم گلبول‌های سفید خون شامل لنفوسیت‌ها، نوتروفیل‌ها و ائوزینوفیل‌ها نداشتند. به طور کلی، افزودن عصاره­های آویشن و سرخارگل به جیره جوجه‌های گوشتی تأثیر معنی‌دار بر شاخص‌های عملکردی و ویژگی‌های لاشه نداشت، ولی باعث افزایش عیار آنتی‌بادی علیه آنفولانزا و کاهش سطح لیپیدهای خون شد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of the Effect of Adding Coneflower and Thyme Extracts to Diet on Performance, Carcass Characteristics, Blood Parameters and Immunity Status of Broiler Chickens

نویسندگان [English]

  • Reza Farrokhnia
  • farid moslemipur
  • shahriar maghsoudlou
  • farzad ghanbari
Gonbad kavoos university
چکیده [English]

Introduction[1]: Nowadays, there is an increasing interest to use organic compounds in broiler chicken diets instead of antibiotics and other feed additives may cause deleterious effects on the poultry products consumers. Using medicinal plants have different effects on broiler life such as improving feed efficiency, augmentation of immunity, producing more desirable carcass and so on. The goal of this study was to investigate the effect of adding coneflower and thyme alcoholic extracts to diet on performance, carcass characteristics, internal organs weights and also some blood and immunity parameters of broiler chickens.
Materials and methods: One hundred-sixty 1-d-old chickens (male and female) were randomly divided into four treatment groups with four replicates in each, and reared for 42 days. The treatments were: 1- control (basal diet formulated according to Cobb500 nutrients recommendation), 2- adding 0.2% thyme extract into basal diet, 3- adding 0.2% coneflower extract into basal diet, and 4- adding 0.2% of thyme+ 0.2% of coneflower extracts into the respective basal diets; starter (1-10 d), grower (11-23 d) and finisher (24-42 d) that were fed to the birds from the beginning. Experimental plants extracts were collected via UV-extraction method by ethanol followed by the rotary evaporation to eliminate the diluent. All the feeding and vaccination programs of chickens were in accordance with Cobb500 commercial strain recommendations. Feed intake, weight gain and feed conversion ratio of chickens were weekly recorded. At the end of the study, one bird of each replicate was slaughtered for carcass analysis and blood sampling. Blood parameters were assayed via spectrophotometer. Carcass parts and internal organs weights were expressed as a percent of carcass weight. The antibody titers against Newcastle and Influenza diseases were assayed. Data were analyzed as a completely randomized design and the means comparison was performed by least significant different test.
Results and Discussion: Results showed that the effect of treatments on the amounts of feed intake and weight gain of chickens over the study. Feed conversion ratio of chickens was significantly affected by the treatments where the lowest was observed in control group and the highest in coneflower group. Carcass traits (the weights of empty carcass, breast, thighs, abdominal fat and back) and internal organs weights (liver, heart, lungs, intestines and gizzard) were not affected by the treatments. The weights of body organs are basically affected by genetic potential that the used chickens were very close in this sight of view, and weight gain also among groups was not significantly different. Effect of treatments on antibody titers against Newcastle and influenza was significant. The lowest antibody titer against Newcastle and the highest against influenza were observed in thyme group. Both of coneflower and thyme were showed having stimulatory effect on antibody production in poultry, but the different effects observed in the present study may be due to the epidemic of diseases and also the hygienic conditions of the farm. Adding coneflower and thyme into the diet increased the bursa of fabricius weight of chickens than the control, but it had no significant effects on spleen weight. It should be mentioned that the chickens in the present study were vaccinated according to the provider company recommendations and also a coccidiostat compound was used in the diet that they together can obstruct or interrupt on the expected beneficial effects of coneflower and thyme on immune parameters. Adding thyme and coneflower into the diet caused a marked decrease in blood lipids concentrations. One of the desired properties of medicinal plants is lowering the blood’s and carcass’s lipids that was observed in the present study. It is reported that these plants have some components suppressing the lipids synthesis in liver and also other ones effect on lipase enzymes activity in tissues. The treatments had no significant effects on the hematological parameters of chickens. Whereas there were no significant differences in the amount of daily feed intake and weight gain of chickens and also all of the environmental conditions for chicken were the same, therefore none of hematological parameters were affected by the treatments.
Conclusion: Generally, adding coneflower and thyme extracts into the diets of chickens had not significant effects on their growth performance in the overall period of the experiment and carcass traits as well. Using coneflower and thyme extracts in broilers diets has a booster effect on the antibody titer against influenza, while no positive effects were observed for Newcastle disease. Adding coneflower and thyme extracts into the diet of chickens prominently decreased the all types of blood lipids. No synergistic effects were observed between coneflower and thyme extracts wherever they had a significant effect on assayed parameters.

 

کلیدواژه‌ها [English]

  • Blood lipids
  • Broiler chicken
  • immunity
  • Performance
  • Medicinal plants
1. Alçiçek, A., M. Bozkurt, and M. Çabuk. 2003. The effect of an essential oil combination derived from selected herbs growing wild in Turkey on broiler performance. South African Journal of Animal Science, 33 (2): 89-94.
2. Al-Kassie, G.A.M. 2009. Influence of two plant extracts derived from thyme and cinnamon on broiler performance. Pakistan Veterinarian Journal, 29 (4): 169-173.
3. Allen, P.C. 2003. Dietary supplementation with Echinacea and development of immunity to challenge infection with coccidian. Animal and Natural Resources Institute, 91: 74-78.
4. Bauer, R. 1996. Echinacea drugs, effects and active ingredients. Zeitschrift Fur ArztlicheFortbildung, 90: 111-115.
5. Chen, H.L., D.F. Li, B.Y. Chang, L.M. Cong, J.D. Dai, and G.F. Yi. 2003. Effects of Chinese herbal polysaccharides on the immunity and growth performance of young broiler. Poultry Science, 82: 364-370.
6. Christake, E., P. Paneri, I. Giannenas, M. Papazahariadou, N.A. Botsoglou, and A.B. Spais. 2004. Effect of mixture of herbal extract on broiler chicken infected with eimeriatenella. Animal Research, 53: 137-144.
7. Debersac, P., M.F. Vernevaut, M.J. Amiot, M. Suschetet, and H. Siess. 2001. Effects of water-soluble extract of thyme and it's purified component in rat liver. Food and Chemical Toxicology, 29: 109- 117.
8. Donaldson, W.E. 1985. Lipogenesis and body fat in chickens. Poultry Science, 64: 1199-1204.
9. Elson, C.E. and Y.G. Yu. 1994. The chemoprevention of cancer by mevalonate-derived constituents of fruits and vegetables. Journal of Nutrition, 124: 607-14.
10. Griffin, D.E. and W.J. Bellini. 2002. Measles virus. In: Knipe DM and Howley PM (Eds.). Fields virology. 4thedn., Philadelphia: Lippincott, Williams and Wilkins, Pp. 1267-312.
11. Jamroz, D., J. Wertlecki, A. Orda, A. Wiliczkiewicz, and J. Skorupińska. 2003. Influence of phatogenic extracts on gut microbial status in chickens. In: Proceedings of 14th European Symposium on Poultry Nutrition, August, Lillehammer, Norway, 176p.
12. Jouri, M.H. and M. Mahdavi. 2010. Practical determining of pasture plants. 1stedition, Ayizh Publication, Tehran, Iran, 436p (In Persian).
13. Kondera, E., A. Dmowska, M. Rosa, and M. Witeska. 2012. The effect of bleeding on peripheral blood and head kidney hematopoietic tissue in common carp (Cyprinuscarpio). Turkish Journal of Veterinary and Animal Sciences, 36 (2): 169-175.
14. Lee, K., H. Everts, and A.C. Beyen. 2003. Dietary carvacrol lowers body gain but improves feed conversion in female broiler chickens. Journal of Applied Poultry Research, 12: 394-399.
15. Lopez-Bote, C.J., J.I. Gray, E.A. Gomaa, and C.J. Flegal. 1998. Effect of dietary administration of oil extracts from rosemary and sage on lipid oxidation in broiler meat. British Poultry Science, 39: 235-240.
16. Luettig, B., C. Steinmüller, G.E. Gifford, H. Wagner, M. and L. Lohmann-Matthes. 1989. Macrophage activation by the polysaccharide arabinogalactan isolated from plant cell cultures of Echinacea purpurea. Journal of the National Cancer Institute, 81(9): 669-75.
17. Mohan, B.R., R. Kadirvel, and M. Bhaskaran. 1996. Effect of prebiotic supplementation on growth nitrogen utilization and serum factors in broilers. Poultry Science, 37: 395-401.
18. Mozaffarian, V. 1986. Glossary of Iran’s plants. 1st edition, Farhang-e-Moaaser Publication, Tehran, Iran, 173p (In Persian).
19. Najafi, P. and M. Torki. 2010. Performance, blood metabolites and immunocompetence of broiler chicks fed diets included essential oils of medicinal herbs. Journal of Animal and Veterinary Advances, 9: 1164-1186.
20. Nasiroleslami, M. and M. Torki. 2010. Including essential oils of Echinacea purpurea and thyme to diet and evaluating performance of laying hens, white blood cell count and egg quality characteristics. Journal of Advances in Environmental Biology, 4 (3): 341-345.
21. Nourizadeh, A., T. Mirzapor, K. Ghasemi, and M. Razavi. 2004. Antibacterial properties of spearmint, thyme, licorice, pennyroyal, camomile and serviceberry. Daneshvar Pezeshki, 52: 67-72 (In Persian).
22. Ocak, N., G. Erener, F. Burak, M. Sungu, A. Altop, and A. Ozmen. 2008. Performance of broilers fed diets supplemented with dry peppermint (Menthapiperita L.) or thyme (Thymus vulgaris L.) leaves as growth promoter source. Czech Journal of Animal Science, 53(4): 169-175.
23. Rahimi, S., Z. TeymouriZadeh, M.A. KarimiTorshizi, R. Omidbaigi, and H. Rokni. 2011. Effect of the three herbal extracts on growth performance, immune system, blood factors and intestinal selected bacterial population in broiler chickens. Journal of Agriculture Science and Technology, 13: 527-539.
24. Rojhan, M.S. 2000. Drugs and herbal medicine. 1stedition Alavi Publication, Tehran, Iran, 311p (In Persian).
25. RoustaeAlimehr, M., B. GhahramaniZahraei, and M. HaghighianRudsari. 2012. Effect of Echinacea purpurea extract on performance, cellular and humoral immune responses of broiler chickens. Iran Veterinary Journal, 9 (2): 60-70 (In Persian).
26. Sarica, S., A. Ciftci, E. Demir, K. Kilinc, and Y. Yıldırım. 2005. Use of an antibiotic growth promoter and two herbal natural feed additives with and without exogenous enzymes in wheat based broiler diets. South African Journal of Animal Science, 35: 61-72.
27. Schulte, K.E., G. Rucker, and J. Perlick. 1967. The presence of polyacetylene compounds in Echinacea purpureaand Chinaceaangustifolia DC. Arzneimittel forschung, 17: 825-829
28. Seemnan, P., D. Cheng, and G.F. Iies. 1973. Structure of membrane holes in, osmotic and saponin hemolysis. Journal of Cell Biology, 56: 519 - 27.
29. Thakar, N.M., D.M. Chairmam, A.R. McElroy, C.L. Novak, and R.L. Link. 2004. Pharmacological screening of some medicinal plants as antimicrobial and feed additives. MSc Thesis. Department of Animal Science. Virginia Polytechnic Institute and State University, Blacksburg, Virginia USA, 73p.