تأثیر افزودن مکمل موننسین و عصاره گیاهی بر رفتار خوردن، تولید و خصوصیات لاشه بره‌های افشاری در حال رشد

نوع مقاله : علمی پژوهشی - تغذیه نشخوارکنندگان

نویسندگان

دانشگاه زنجان

چکیده

این پژوهش جهت بررسی اثر افزودن موننسین و عصاره گیاهی در جیره‌های با کنسانتره بالا بر رفتار و عملکرد بره‌های افشاری انجام گرفت. برای انجام این آزمایش از 32 رأس بره نر افشاری با میانگین وزن اولیه 6/5 ± 41 کیلوگرم و سن شش ماه در قالب طرح کاملاً تصادفی با چهار تیمار و هشت تکرار به مدت 56 روز دوره آزمایشی و 21 روز سازگاری به جیره آزمایشی استفاده شد. جیره‌های آزمایشی شامل؛ 1) جیره شاهد بدون افزودن موننسین یا عصاره گیاهی، 2) جیره حاوی 30 میلی‌گرم موننسین، 3) جیره حاوی دو گرم عصاره گیاهی، 4) جیره حاوی 30 میلی‌گرم موننسین به صورت دو هفته در میان برای هر رأس بره بود. وزن کشی به صورت هفتگی و خوراک مصرفی به طور روزانه اندازه‌گیری شد. بررسی رفتارهای خوردن و نشخوار کردن و تعیین pH شکمبه هر دو هفته یکبار انجام شد. در پایان دوره بره‌ها کشتار شدند و تفکیک و تجزیه لاشه صورت گرفت. نتایج حاصل از این آزمایش نشان داد که افزودن مکمل موننسین می‌تواند اثرات مثبتی بر افزایش وزن، خصوصیات لاشه و رفتارهای تغذیه‌ای بره‌های پرواری داشته باشد، ولی اثرات افزودن مکمل موننسین در طول پروار ثابت نبود و در اغلب پاسخ‌ها اثر جیره در دوره معنی‌دار بود به‌علاوه مشاهد شد که افزودن مکمل موننسین بصورت یک دوره در میان می‌تواند پاسخ‌های متفاوتی از افزودن آن به طور مستمر داشته باشد همچنین افزودن مکمل عصاره گیاهی به میزان دو گرم در روز تأثیر اندکی بر پاسخ‌های بره‌های پرواری داشت.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Monensin and Plant Extract Supplementation on Feeding Behaviors, Production and Carcass Characteristics of Afshari Lambs

نویسندگان [English]

  • Hamidreza Mirzaei alamouti
  • Zahra Shahalizadeh
  • hamid amanlou
  • mostafa hajilou
  • kamran akbari pabandi
zanjan univercity
چکیده [English]

Introduction The ionophore antibiotics have been added to high-grain diets as feed additives and reduced digestive problems with this compound have been reported in many studies. Monensin is an important ionophore that has been used for approximately four decades based on its potential to control rumen fermentation pattern and to decrease the incidence of acidosis and increase efficiency of nutrient utilization. These ionophores facilitate the release of ions from the lipid membrane of bacteria and protozoa also ionopheres increase propionate and decrease methane production. However, the use of antimicrobial agents as feed additives can contribute to antimicrobial resistance both in animals and humans, posing a serious risk to the public health. More recently, there has been a growing interest in the potential use of plant extracts as alternatives for antimicrobial feed additives in ruminants. The essential oils are second metabolites of plants, which have some effects on ruminal protein degradation and mitigate methane production. We hypothesized that replacing the plant extract for monensin would affect behavior and performance of Afshari lambs fed high concentrate diet.
Materials and Methods Thirty two Afshari lambs with an average initial weight of 41 ± 5.6 kg and six months old were used in a completely randomized design with four treatments and eight replications in each treatment for 56 days with 21 days as an adaptation period and 35 days as an experimental period. In these periods, four diets for feedlot lambs were given. The basal diet was contained 20 % alfalfa hay, 65 % ground barley and 11% soybean meal. The basal diet was formulated with Cornell model software. Crude protein and metabolizable energy were 16 % and 2.75 Mcal/kg of dry matter respectively. The experimental diets were; 1) basal diet without additives, monensin or essential oil, 2) basal diet with 30 mg monensin/d for each lamb, 3) basal diet with two grams of a commercial plant extract, and 4) basal diet with periodically inclusion of 30 mg monensin/d for each lamb for two weeks and no for next two weeks. The lambs were weighed weekly and feed intake was measured daily. Feeding behavior and rumination and rumen pH determination was performed every two weeks. At the last day the lambs were slaughtered and the carcasses characteristics were determined.
Results and Discussion Differences in dry matter intake did not differ due to relatively large individual variation in voluntary intake, but it tended to lower (P = 0.06) with monensin fed lambs. Effect of monensin on feed intake varied over time. The increases in feed efficiency and propionate production have hypothesized in many studies for decreasing DMI with monensin supplementation. Monensin can increase propionate production and its supply to liver and it has a hypophagic effect. Monensin inclusion to basal diet result to increase the time spent eating, chewing activity and the mean of eating meals, and also it increased eating and chewing activity for each kg of dry matter intake (P < 0.05), and however, these effects were not observed with periodical inclusion of monensin. It is reported that monensin had an effect on rumen fermentation and it can reduce absorption of nutrients during a meal, so the meal size is reduced. Lambs fed high concentrate diets were adapted and had decreased rumen pH fluctuations when monensin was included in diet. Daily weight gain and slaughter weight was not significantly different between treatments (P > 0.05). The lambs fed monensin periodically had numerically lowest weight gain and monensin fed lambs had greater hot and cold carcass weight. Proposed that increased propionic acid as a result of the effects of monensin may be used in gluconeogenesis, thus it spared the amino acids for glucose and indirectly increase muscle or protein accretion. In addition, monensin stimulates micro-organisms in the digestive tract, so it optimizes nutrient synthesis in rumen and improves nutrient absorption in the digestive system.
Conclusion The results showed that periodically inclusion of monensin to lambs diet may compromise performance compared to daily inclusion of monensin and plant extract. Inclusion of plant extract had a positive effect on daily weight gain and could be replaced for monensin, but further research is need to warrant these effects and ruminal and metabolic aspects of plant extracts and monensin.

کلیدواژه‌ها [English]

  • Essential oil
  • Finishing lamb
  • High grain diet
  • Monensin
1- Safayi, Kh. 2003. Effect of monensin levels in finishing diet of Gezel sheeps by Step-up feeding method. MSc Thesis. University of Tabriz, Iran. (In Persian).
2- Ataei Varjoy, A, and Y. Ebrahimnezhad. 2009. The effects of ionosphere supplement (salinomycin and semdoramycin) on performance, growth rate, and feed conversion and carcass characteristics of broiler chickens. Journal of Agricultural Sciences, 12(3): 95-105. (In Persian).
3- Noorollahi, H. 2006. Effect of finishing Term on growth and carcass characteristics of Turkic Qashqai lambs. Fars Research Center for Agriculture and Natural Resources. Livestock and aquaculture organization. (In Persian).
4- Allen, M. S. 2000. Effect of diet on short term regulation of feed intake by dairy cattle. Journal of Dairy Science, 83:1598-1624.
5- Baran, M. 1988. Rumen fermentation in sheep given both monensin and pectinase. Journal of Veterinary Medicine, 33: 5289-296.
6- Beckett, S., I. Lean., R. Dyson., W. Tranter, and L. Wade. 1998. Effects of monensin on the reproduction, health, and milk production of dairy cows. Journal of Dairy Science, 81: 1563–1573.
7- Benchaar, C., H. V. Petit., R. Berthiaume., T. D. Whyte, and P. Y. Chouinard. 2006. Effects of addition of essential oils and monensin premix on digestion, ruminal fermentation, milk production, and milk composition in dairy cows. Journal of Dairy Science, 89: 4352-4364.
8- Benchaar, C., S. Calsamiglia, A.V. Chaves, G. R. Fraser, D. Colombatto, T. A. McAllister, and K. A. Beauchemin. 2008. A review of plant-derived essential oils in ruminant nutrition and production. Journal of Animal Feed Science and Technology, 145: 209–228.
9- Bergen, W. G, and D. B. Bates. 1984. Ionophores: their effect on production efficiency and mode of action. Journal of Animal Science, 58: 1465–1483.
10- Broderick, G. A. 2004. Effect of low level monensin supplementation on the production of dairy cows fed alfalfa silage. Journal of Animal Science. 87:359-368.
11- Busquet, M., S. Calsamiglia., A. Ferret., M. D. Carro, and C. Kamel. 2005. Effect of garlic oil and four of its compounds on rumen microbial fermentation. Journal of Dairy Science, 88: 4393-4404.
12- Calhoun, M. C., L. H. Carroll., C. W. Livingston, and S. Maurice. 1979. Effect of dietary monensin on coccidial oocyst numbers, feedlot performance and carcass characteristics of lambs. Journal of Animal Science, 49: 10-19.
13- Castillo, C., J. L. Benedito., J. Mendez., V. Pereira., M. Lopez-Alonso., M. Miranda, and J. Hernandez. 2004. Organic acids as a substitute for monensin in diets for beef cattle. Animal Feed Science and Technology, 115: 101-116.
14- Deswysen, A. G., W. C. Ellis., K. R. Pond., W. L. Jenkins, and J. Connelly. 1987. Effects of monensin on voluntary intake, eating and ruminating behavior and ruminal motility in heifers fed corn silage. Journal of Animal Science, 64: 827-834.
15- Erickson, G. E., C. T. Milton., K. C. Fanning., R. J. Cooper., R. S. Swingle., J. C. Parrott., G. Vogel, and T. J. Klopfenstein. 2003. Interaction between bunk management and monensin concentration on finishing performance, feeding behavior, and ruminal metabolism during an acidosis challenge with feedlot cattle Journal of Animal Science, 81: 2869-2879.
16- Forbes, J. M. 1980. A model of the short term control of feeding in the ruminant: Effect of changing animal or feed characteristics. Appetite, 1(1): 21-41.
17- Gonzalez-Momitaa, M. L., J. R. Kawas., R. Garcia., C. Castillo., J. Gonzalez-Morteo., G. Aguirre-Ortega., H. Hernandez-Vidal., F. J. Fimbres-Durazo, and C. D. Pican-Rubio. 2009. Nutrient intake, digestibility, mastication and ruminal fermentation of pelibuey lambs fed finishing diets with ionophore (monensin or lasalocid) and sodium malate. Journal of Small Ruminant Research, 83: 6-11.
18- Goodrich, R. D., J. E. Garrett., D. R. Gast., M. A. Kirick., D. A. Larson, and J. C. Meiske. 1984. Influence of monensin on the performance of cattle. Journal of Animal Science, 58: 1484–1498.
19- Harmon, D. L., K. K. Kreikemeier, and K. L. Gross. 1993. Influence of addition of monensin to an alfalfa hay diet on net portal and hepatic nutrient flux in steers. Journal of Animal Science, 71: 218 – 225.
20- Heydari, K. H., N. Dabiri., J. Fayazi, and H. Roshanfekr. 2008. Effect of Ionophores monensin and lasalocid on performance and carcass characteristics in fattening Arabi lambs. Pakistan Journal of Nutrition, 7(1): 81-84.
21- Hobson, P. N. 1997. The rumen microbial ecosystem. Elsevier Applied Science, London and New York.
22- Itavo, C. C. B. F., M. G. Morais., C. Costa., L. C. V. Itavo., G. L. Franco., J. A. Silva, and F. A. Reis. 2011. Addition of propolis or monensin in the diet: Behavior and productivity of lambs in feedlot. Animal Feed Science and Technology, 165: 161-166.
23- Khan, M. D. 2005. Dose titration, tolerance and compatibility of some feed additives in broiler. PhD Thesis. University of Hohnheim, Dhaka, Bangladesh.
24- Martini, M., P. Verita., F. Cecchi, and D. Cianic. 1996. Monensin sodium use in lambs from the second week of life to slaughter of 105 days. Journal of Small Ruminant Research, 20: 1-8.
25- Martini, M., P. Verita, L. Cecchi, and D. Cianci. 1991. Monensin sodium use in lambs from the second week of life to slaughter at 105 days. Small Ruminant Research, 20(1): 1-8.
26- Meyer, N. F., G. E. Erickson., T. J. Klopfenstein., M. A. Greenquist., M. K. Luebbe., P. Williams, and M. A. Engstrom. 2009. Effect of essential oils, tylosin, and monensin on finishing steer performance, carcass characteristics, liver abscesses, ruminal fermentation, and digestibility. Journal of Animal Science, 87: 2346-2354.
27- Mirzaei, F., M. Rezaeian., A. Towhidi., A. Nik-khah, and H. Sereshti, 2009. Effects of fish oil, safflower oil and monensin supplementation on performance, rumen fermentation parameters and plasman metabolites in Chall sheep. International Journal of Veterinary Research, 3(2): 113-128.
28- Morris, F. E., M. E. Branine., M. L. Galyean., M. E. Hubbert., A. S. Freeman, and G. P. Lofgreen. 1990. Effect of rotating monensin plus tylosin and lasalocid on performance, ruminal fermentation, and site and extent of digestion in feedlot cattle. Journal of Animal Science, 68: 3069-3078.
29- Murphy, M. A., H. N. Zerby, and F. L. Fluharty. 2003. The effects of energy source and ionophore supplementation on lamb growth, carcass characteristics and tenderness. Sheep and Goat Research Journal, 18: 89-95.
30- Muwalla, M. M., M. N. Abo-Shehada, and F. Tawfiq. 1994. Effects of monensin on daily gain and natural coccidial infection in Awassi lambs. Journal of Small Ruminant Research, 13: 205-209.
31- Nagaraja, T. G. 1995. Ionophores and antibiotics in ruminants. Pages 173-204 In Biotechnology in animal feeding. R. J. Wallace, and A. Chesson, ed. VCH Publishers, New York.
32- Nagaraja, T. G., C. J. Newbold., C. J. Van Nevel, and D. I. Demeyer. 1997. Manipulation of rumen fermentation. Pages 523-632 in The rumen microbial ecosystem. Hobson, P. N. ed. Elsevier Applied Science, London and New York.
33- Nockels, C. F., D. W. Jackson, and B. W. Berry. 1978. Optimum level of monensin for fattening lambs. Journal of Animal Science, 47(4): 788-790.
34- NRC. 2007. Nutrient requirements of small ruminants. National Academy press, Washington, DC.
35- Oliveira, M. V. M., R. P. Lana., E. C. Eifert., D. F. Luz., J. C. J. R. O. Pereira., F. M. Perez, and J. Vargas. 2007. Effect of monensin on intake and apparent digestibility of nutrients in sheep fed diets with different crude protein levels. Revista Brasileira de Zootecnia, 36(3): 643–651.
36- Potter, E. L., A. P. Raun., C. O. Cooley., R. P. Rathmacherand, and L. F. Richardson. 1976. Effect of monensin on carcass characteristics, carcass composition and efficiency of converting feed to carcass. Journal of Animal Science, 43(3): 678-683.
37- Simpson, M. E., P.B. Marsh, and D. A. Dinius, 1976. Effect of monensin and other antibiotics on in vitro digestion of cellulosic substrates in ruminal fluid from steers not previously exposed to antibiotics. Proc. Notheast Sec. ASAS.
38- Taghipoor, B., H. A. Seifi., M. Mohri., N. Farzaneh, and A. A. Naserian. 2010. Effect of prepartum administration of monensin on metabolism of pregnant ewes. Journal of Livestock Science. 135:231-237.
39- Thornton, J. H, and F. N. Owens. 1995. Monensin supplementation and in vivo methan production by steers. Journal of Animal Science, 52(3): 628-634.
40- Van Baale, M. J., J. M. Sargeant., D. P. Gnad., B. M. Debey., K. F. Lechtenbery, and T. G. Nagaraja. 2004. Effect of forage or grain diets with or without monensin on ruminal persistence and fecal Escherichia coli o 157:H7 in cattle. Applied and Environmental Microbiology, 70(9): 5336–5342.
41- Vieira Salles, M. S., M. A. Zanetti., E. A. Lencioni Titto, and R. M. Consentino Conti. 2008. Effect of monensin on performance in growing ruminants reared under different environmental temperatures. Journal of Feed Science and Technology, 147: 279–291.
42- Yang, W. Z., C. Benchaar., B. N. Ametaj., A. V. Chaves., M. L. He, and T. A. McAlliater, 2007. Effects of garlic and juniper berry essential oils on ruminal fermentation and on the site and extent of digestion in lactating cows. Journal of Dairy Science, 90: 5671-5681.