تعیین ترکیب اسیدهای چرب سیس و ترانس گوشت و دنبه گوسفندان بلوچی در استان خراسان جنوبی

نوع مقاله : علمی پژوهشی - تغذیه نشخوارکنندگان

نویسندگان

1 گروه علوم دامی، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران.

2 علوم پزشکی بیرجند

3 گروه شیمی، دانشگاه بیرجند، بیرجند، ایران.

چکیده

به منظور تعیین ترکیب اسیدهای چرب دنبه و گوشت گوسفندان بلوچی استان خراسان جنوبی، تعداد 5 نمونه گوشت ران و راسته و 5 نمونه دنبه از 5 رأس گوسفند از محل کشتارگاه های دام، هشت منطقه استان خراسان جنوبی یعنی شهرستان های بیرجند، سربیشه، نهبندان، درمیان، قائن، فردوس، سرایان و بشرویه (از 40 رأس گوسفند در سطح استان) تهیه و جمع آوری گردید و نمونه ها تا زمان استخراج چربی آن ها در فریزر oC80- نگهداری شد. پس از جمع آوری تمام نمونه ها، چربی استخراج و متیله گردید سپس نوع و درصد اسیدچرب در مقایسه با زمان پیک و مقدار پیک استاندارد و روش استاندارد داخلی تعیین گردید و سپس آنالیز آماری داده ها به کمک نرم افزار SAS انجام شد. تجزیه و تحلیل داده ها نشان داد درصد مجموع اسیدهای چرب اشباع دنبه گوسفندان از گوشت بالاتر بود (05/0>P). مجموع درصد اسیدهای چرب غیراشباع با یک پیوند دوگانه و غیراشباع با چند پیوند دوگانه در گوشت بالاتر از چربی بود (05/0>P). درصد اسیدهای چرب امگا-3 در گوشت بالاتر از دنبه ولی اختلاف آن‌ها معنی‌دار نبود. درصد اسیدهای چرب امگا-6 و اسیدهای چرب ترانس در دنبه بطور معنی‌داری بالاتر از گوشت گوسفندان بود (05/0>P). نسبت اسیدهای چرب غیراشباع با چند پیوند دوگانه به اسیدهای چرب اشباع (PUFA/SFA) در گوشت بالاتر از دنبه گوسفندان بود. در مجموع تحلیل داده‌ها نشان می‌دهد که هر چند درصد مجموع اسیدهای چرب ترانس دنبه گوسفندان بالاتر بود ولی از شاخص استاندارد ایران پائین‌تر می‌باشد. تحلیل ترکیب اسیدهای چرب گوشت و دنبه گوسفندان مناطق مختلف استان خراسان جنوبی بدلیل تفاوت در ترکیب چراگاه مورد استفاده با هم اختلاف معنی‌داری نشان داد. از طرف دیگر با توجه به نسبت بالای اسیدهای چرب غیراشباع و پائین تر بودن درصد اسیدهای چرب اشباع گوشت در مقایسه با دنبه، کیفیت گوشت از دنبه گوسفندان استان خراسان جنوبی بهتر می‌باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation the cis and trans Fatty Acid Composition in the Sheep's Fat-Tail and Meat of Baluchi Sheep in South Khorasan Province

نویسندگان [English]

  • seyyed javad Hosseini-vashan 1
  • Mohammad Malekaneh 2
  • Ali Allahressani 3
1 Department of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran.
2 Birjand University of Medical Sciences
3 Department of Chemistry, Birjand University, Birjand, Iran.
چکیده [English]

Introduction The common isoform of fatty acids in animal and herbal lipids are cis fatty acids, however in some sources such as milk and meat of ruminant, the trans fatty acids are more abundant as compared to the plant lipids. The trans fatty acids are very important because they increase the cardiovascular and heart attack. The heart disorders were increased by enhancing the concentration of omega-6 fatty acids and decreasing the concentration of omega-3 fatty acids in ration and blood. The types of pasture cause alter the fatty acid composition of lamb meat. For evaluation of heart function and health of heart, the artherosclosis and thrombogensis were calculated by the concentration of saturated and unsaturated fatty acids. Therefore, the subject of this study, evaluation of the fatty acid profiles of sheep's fat-tail and meat at different places of South Khorasan Province.
Material and Methods Five samples of meat and fat-tail from the animal's abattoir in Birjand, Sarbisheh, Asadieh, Ferdows, Saraian, Ghaen, Boshroieh and Nehbandan cities were collected and frozen (40 samples of meat and fat-tail). The samples were frozen in -80oc freezer. Then, the oils were separated and the fatty acids were extracted and methylated by the method of Morrison and Smith (1964). After that, the 0.5 µl of methylated fatty acids were injected to gas chromatography instruments (Varian 4200). The capillary column CPSill 88 was used. The type of each fatty acid was designated with compare to the retention time in the standard peak. The quantity of fatty acids was determined by internal standard method. To evaluation the fatty acids ratio for health, the artherosclosis and thrombogensis indices were calculated based on saturated and unsaturated fatty acids. The data were analyzed by SAS software, and the mean were compared by tukey test (α=0.05).
Results and Discussion The analysis of data revealed that the percentage of saturated fatty acids (SFA) was higher at fat-tail as compared to meat of sheeps. The meat had higher MUFA and PUFA (P

کلیدواژه‌ها [English]

  • Cis and Trans fatty acids
  • Fat-tail
  • Meat sheep
  • South Khorasan Province
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