اثر مکمل روغن کتان و تزریق ویتامین‌های (A, D3, E) بر عملکرد و متابولیت‌های سرم گوساله‌های ماده شیر‌خوار

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه علوم دامی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

به‌منظور بررسی تأثیر روغن دانه کتان و ویتامین‌های محلول در چربی (E, D3, A) بر عملکرد و فراسنجه‌های خونی گوساله‌های شیرخوار، آزمایشی با استفاده از 28 رأس گوساله شیرخوار ماده هلشتاین اجرا شد. در این آزمایش، گوساله‌ها در سن چهار روزگی به‌صورت کاملاً تصادفی به یکی از چهار تیمار مورد آزمایش اختصاص داده شدند. تیمار‌های مورد آزمایش شامل: 1) شیرکامل همراه با استارتر آغازین (شاهد) 2) جیره شاهد مکمل شده با روغن کتان (3/0میلی لیتر به‌ازای هر کیلو وزن بدن)، 3) جیره شاهد و ترزیق هفته‌ای هفت سی‌سی ویتامین محلول در چربی 4) جیره شاهد مکمل شده با روغن کتان (3/0 میلی لیتر به‌ازای هر کیلو وزن بدن) و ترزیق هفته‌ای هفت سی‌سی ویتامین محلول در چربی. نمونه‌های خون در روز‌های 1، 14، 28، 56 روزگی (زمان از شیر گیری) از سیاه رگ گردنی جمع‌آوری شد. نتایج نشان داد که تیمارهای آزمایشی تأثیری بر مصرف خوراک، افزایش وزن روزانه، بازده خوراک و رشد اسکلتی نداشت. غلظت پارامتر‌های سرم خونی مانند گلوکز، پروتئین تام، کراتنین، تری گلیسیرید و اوره سرمی خون تحت تأثیر تیمار‌های آزمایشی قرار نگرفتند. نتایج به‌دست آمده از این آزمایش نشان می‌دهد که استفاده از اسیدهای چرب اشباع نشده چندگانه روغن دانه کتان و یا تزریق ویتامین محلول در چربی نه تنها هیچ تأثیر سوئی بر عملکرد گوساله‌ها نداشت، بلکه سبب بهبود برخی از متابولیت سرمی نیز شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Effect of Flaxseed Oil Supplementation and Injection of Vitamins (A, D3, E) on the Performance and Blood Metabolites of Suckling Calves

نویسندگان [English]

  • Mehrdad Movahednasab
  • Abdolmansour Tahmasebi
  • Seyed Alireza Vakili
  • abasali naserian
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
چکیده [English]

Introduction: Proper nutrition management during calf rearing is one of the most important factors which influence herd profitability. Environmental stressors and immature immune system of calves, reduce the viability of the animal. Today, attempts are being made to increase immune system postnatal calf life in various ways. Supplying unsaturated fats, which have double bonds on their structure, in starter diet, is one of the suggested solutions to improve the calf’s immune system. Infant ruminants do not have the ability to biosynthesize fat-soluble vitamins (A, D3, E), while these vitamins play a major role in the animal's immune system and their performance. Supplying fat-soluble vitamins in the calves’ diets is critical for the normal growth of muscles and the body skeleton, as they have an improvement role to improve the immune system in animals. The common symptoms of a fat soluble vitamin deficiency in suckling calves are growth retardation, coarse hair, and susceptibility to infectious diseases.
Materials and Methods: In this experiment, twenty eight Holstein female calves with an average weight of 37.74 kg (± 4/76) were used from birth to 56 days. After birth claves were separated from their dam and after weighing they transferred to individual pen. On the fourth day, the calves were randomly assigned to one of four treatments.  All calves received colostrum for the first 3 d and then whole milk at 8% of bodyweight in the two equal part in the morning (4.00 A.M) and evening (16 P.M) until weaning. The experimental treatments included: 1) control: whole milk with starter 2) control diet supplemented with flaxseed oil (0.3ml per kilogram of body weight) 3) control diet and weekly injection of 7 cc of fat-soluble vitamins (A, D3, E) 4) The control diet which supplemented with flaxseed oil (0.3ml per kilogram of body weight) plus weekly injection of 7 cc of fat-soluble vitamins.  Flaxseed oil was mixed into milk (morning feeding) until weaning. During the experiment period, calves had ad libitum access to chopped alfalfa hay and starter diet. Water from a plastic bucket (7 liters), filled twice a day, and was provided throughout the study. Composition of starter did not change throughout the experiment. Starter intake was recorded daily. Body weight gain determined weekly until the end of experiment. Fecal consistencies were scored twice a day. Blood sample were harvested from jugular vein for collection of blood serum. Blood metabolites, glucose, cholesterol, triglycerides were analyzed using commercially available colorimetric and enzymatic assay kits. Data were analyzed using SAS version 9.4 as a randomized completely randomized design experiment. For all results, significant differences between treatments were reported at P≤ 0.05 and tendencies were reported at 0.05 < P≤ 0.10. Least square means for each treatment are reported in the tables and were separated using Tukey’s test.
Results and Discussion: The results of this study showed that the use of flaxseed oil and injection of fat-soluble vitamins during pre-weaning period had no significant effect on the starter feed intake, daily weight gain, rectal temperature and skeletal growth parameters of calves.
Serum concentrations of total protein, triglyceride, creatinine and urea were not affected by experimental treatments. Claves received flaxseed oil and fat soluble vitamins injection tended to have the lowest serum glucose concentration (P<0.10). Calves received flax seed oil and fat soluble vitamins injection had the highest cholesterol concentration.
Conclusion: The results of this study indicated that inclusion of flaxseed oil and injection of fat-soluble vitamins (A, D, E) had no any marked effects on weight gain, daily feed intake and skeletal bone growth.

کلیدواژه‌ها [English]

  • Calf
  • Fat-soluble vitamins
  • Flaxseed oil
  • Unsaturated fatty acids
  1. Abd Elghany Hefnawy, R., Revilla-Vazquez, , Ramírez-Bribiesca, E., & Tórtora-Pérez, J. (2008). Effect of pre-and postpartum selenium supplementation in sheep. Journal of Animal and Veterinary Advances7(1), 61-67.
  2. Ambrose, D. J., Kastelic, J. P., Corbett, R., Pitney, P. A., Petit, H. V., Small, J. A. & Zalkovic, P., (2006). Lower pregnancy losses in lactating dairy cows fed a diet enriched in α-linolenic acid. Journal of Dairy Science, 89(8), 3066-3074.
  3. Blakely, L. P., Poindexter, B., Stuart, R, L., & Nelson, C. D. (2019). Supplementing pasteurized waste-milk with vitamins A, D, and E improves vitamin status of dairy calves. The Bovine Practitioner, 134-141.
  4. Doppenberg, J., & Palmquist, D. L. (1991). Effect of dietary fat level on feed intake, growth, plasma metabolites and hormones of calves fed dry or liquid diets. Livestock Production Science, 29(2-3), 151-166.
  5. El-Diahy, Y. M., El-Hamd, A., & Elshora, M.A. (2016). Effect of flaxseed oil supplementation during pre and postpartum on some physiological parameters and productive performance cow. Egyptian Journal of Nutrition and Feeds, 19(1), 1-15.
  6. Ghorbani, H., Kazemi-Bonchenari, M., HosseinYazdi, M., & Mahjoubi, E. (2020). Effects of various fat delivery methods in starter diet on growth performance, nutrients digestibility and blood metabolites of Holstein dairy calves. Animal Feed Science and Technology 262, 114429.
  7. Garcia, M., Shin, J. H., Schlaefli, A., Greco, L. F., Maunsell, F. P., Santos, J. E. P., Staples, C. R., & Thatcher, W. W., (2015). Increasing intake of essential fatty acids from milk replacer benefits performance, immune responses, and health of preweaned Holstein calves. Journal of dairy science, 98(1), 458-477.
  8. Ghasemi, E., Azad-Shahraki, M., & Khorvash, M. (2017). Effect of different fat supplements on performance of dairy calves during cold season. Journal of Dairy Science, 100(7), 5319-5328.
  9. Godden, S. M., Haines, D. M., Konkol, K., & Peterson, J. (2009). Improving passive transfer of immunoglobulins in calves. II: Interaction between feeding method and volume of colostrum fed. Journal of dairy science, 92(4), 1758-1764.
  10. Hill, T. M., VandeHaar, M. J., Sordillo, L. M., Catherman, D. R., Bateman Ii, H. G., & Schlotterbeck, R. L. (2011). Fatty acid intake alters growth and immunity in milk-fed calves. Journal of Dairy Science, 94(8), 3936-3948.
  11. Hill, T. M., Aldrich, J. M., Schlotterbeck, R. L., & Bateman Ii, H. G. (2007). Amino acids, fatty acids, and fat sources for calf milk replacers. The Professional Animal Scientist, 23(4), 401-408.
  12. Hill, T. M., Bateman II, H. G., Aldrich, J. M., & Schlotterbeck, R.L. (2009). Effects of changing the essential and functional fatty acid intake of dairy calves. Journal of Dairy Science, 92(2), 670-676.
  13. Kadkhoday, A., Ahmad, R., Masoud, , Dehghan-Banadaky, M., & Kowsar, R. (2017). Effects of fat sources and dietary C18: 2 to C18: 3 fatty acids ratio on growth performance, ruminal fermentation and some blood components of Holstein calves. Livestock Science, 204, 71-77.
  14. Karcher, E. L., Hill, T. M., Bateman II, H. G., Schlotterbeck, R. L., Vito, N., Sordillo, L. M. & Vandehaar, M.J., (2014). Comparison of supplementation of n-3 fatty acids from fish and flax oil on cytokine gene expression and growth of milk-fed Holstein calves. Journal of Dairy Science, 97(4), 2329-2337.
  15. Karimi, A., Alijoo, Y. A., Kazemi-Bonchenari, M., Mirzaei, M., & Sadri, H. (2021). Effects of supplemental fat sources and forage feeding levels on growth performance, nutrient digestibility, ruminal fermentation, and nitrogen utilization in dairy calves. Animal,
  16. Khan, M. A., Lee, H. J., Lee, W. S., Kim, H. S., Kim, S. B., Ki, K. S., Park, S. J., Ha, J. K., & Choi, Y. J. (2007). Starch source evaluation in calf starter: I. Feed consumption, body weight gain, structural growth, and blood metabolites in Holstein calves. Journal of Dairy Science, 90(11), 5259-5268.
  17. Khan, M. A., Lee, H. J., Lee, W. S., Kim, H. S., Kim, S. B., Park, S. B., Baek, K. S., Ha, J. K., & Choi, Y. J. (2008). Starch source evaluation in calf starter: II. Ruminal parameters, rumen development, nutrient digestibilities, and nitrogen utilization in Holstein calves. Journal of Dairy Science, 91(3), 1140-1149.
  18. Krueger, L. A., Reinhardt, T. A., Beitz, D. C., Stuart, R. L. & Stabel, J. R. (2016). Effects of fractionated colostrum replacer and vitamins A, D, and E on haptoglobin and clinical health in neonatal Holstein calves challenged with Mycobacterium avium paratuberculosis. Journal of Dairy Science, 99(4), 2884-2895.
  19. Masmeijer, C., Van Leenen, K., De Cremer, L., Deprez, P., Cox, E., Devriendt, B., & Pardon, B. (2020). Effects of omega-3 fatty acids on immune, health and growth variables in veal calves. Preventive Veterinary Medicine, 179, 104979.
  20. McCoard, S., Heiser, A., Lowe, K., Molenaar, A., MacLean, P., Johnstone, P., Leath, S., Hoskin, S.O., & Khan, M.A. (2019). Effect of weaning age on growth, mammary gland development, and immune function in Holstein Friesian calves fed conserved alfalfa (FiberStart). Journal of Dairy Science, 102(7), 6076-6087.
  21. McDowell, Lee, R. (2008). Vitamins in animal and human nutrition. John Wiley and Sons.
  22. McDonnell, R. P., O’Doherty, J. V., Earley, B., Clarke, A. M., & Kenny, D. A. (2019). Effect of supplementation with n-3 polyunsaturated fatty acids and/or β-glucans on performance, feeding behaviour and immune status of Holstein Friesian bull calves during the pre-and post-weaning periods. Journal of Animal Science and Biotechnology, 10(1), 1-17.
  23. McGuirk, S. M. (2005). Otitis media in calves. Proc. 23rd Coll. Vet. Intern. Med., Baltimore, MD: 228-230.
  24. Newkirk, R. (2015). Flax feed industry guide. Flax Canada, 1-24.
  25. Nonnecke, B. J., Foote, M. R., Miller, B. L., Beitz, D. C., & Horst, R. L. (2010). Fat-soluble vitamin and mineral status of milk replacer-fed dairy calves: Effect of growth rate during the preruminant period. Journal of Dairy Science, 93(6), 2684-2690.
  26. National Research Council, (2001). Nutrient requirements of dairy cattle: 2001. National Academies Press.
  27. Pearce, S. C., Gabler, N. K., Ross, J. W., Escobar, J., Patience, J. F., Rhoads, R. P., & Baumgard, L. H. (2013). The effects of heat stress and plane of nutrition on metabolism in growing pigs. Journal of Animal Science, 91(5), 2108-2118.
  28. Quigley, J. D., Wolfe, T. A., & Elsasser, T. H. (2006). Effects of additional milk replacer feeding on calf health, growth, and selected blood metabolites in calves. Journal of Dairy Science, 89(1), 207-216.
  29. Schneider, P. L., Beede, D. K., & Wilcox, C. J. (1988). Nycterohemeral patterns of acid-base status, mineral concentrations and digestive function of lactating cows in natural or chamber heat stress environments. Journal of Animal Science, 66(1), 112-125.
  30. Śpitalniak-Bajerska, K., Szumny, A., Pogoda-Sewerniak, K., & Kupczyński, R. (2020). Effects of n-3 fatty acids on growth, antioxidant status, and immunity of preweaned dairy calves. Journal of dairy science, 103(3), 2864-2876.
  31. Thiessen Debbie, L. (2004).Optimization of feed peas, canola and flaxseed for aqua feeds: The Canadian prairie perspective. Avancesen Nutricion Acuicola.
  32. Winter, K. A. (1985). Comparative performance and digestibility in dairy calves weaned at three, five, and seven weeks of age. Canadian Journal of Animal Science, 65(2), 445-450.
  33. Yunianto, V. D., Hayashit, K., Kaneda, S., Ohtsuka, A., & Tomita., Y (1997). Effect of environmental temperature on muscle protein turnover and heat production in tube-fed broiler chickens. British Journal of Nutrition, 77(6), 897-909.
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