Comparing proteins and carbohydrates molecular structures in different sorghum cultivars using fourier transform infrared spectroscopy (FTIR) and multivariate analyses

Document Type : Ruminant Nutrition


1 Ferdowsi university of mashhad

2 Ferdowsi university of Mashhad

3 Ferdowsi University of Mashhad

4 University of Saskatchewan


This study was carried out to determine the protein and carbohydrate molecular structure of sorghum cultivars using Fourier Transform Infrared Spectroscopy (FTIR) with multivariate molecular spectroscopy analyses. Sorghum cultivars included: 1- Kimia, 2- Sepideh, 3- M2 and 4- M8. Protein and carbohydrate molecular functional groups studied included: peak area and height amide I, amide II, α-helix, β-sheet, 860 (non-structure carbohydrate), 928 (non-structure carbohydrate), total carbohydrate (CHO) with three major component peaks in this region, cellulosic compounds and different ratio of molecular structure. FTIR results showed that there were significant differences between sorghum cultivars in terms of proteins and carbohydrates molecular structures. Kimia had the greatest peak area and height amide I, II, α-helix, β-sheet, total carbohydrate and cellulosic compounds. Sepideh, M2 and M8 had similar proteins and carbohydrates molecular structures. Differences in protein and carbohydrate molecular structures can influence the availability of proteins and carbohydrates in ruminant and monogastric. Further studies needed to understand the effect of variety on protein and carbohydrate structure of sorghum and the relationship between protein and carbohydrate structure of a feed with nutrient availability in ruminant and monogastric


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