Determination of Metabolizable Energy of Corn Processed at Different Temperatures and Effect of their Inclusion in Mash Diets with and without Enzyme Supplementation on Growth Performance and Small Intestine Morphology of Broiler Chickens During 1-24 days

Document Type : Poultry Nutrition

Authors

1 Department Animal Science, Faculty of agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

2 Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

3 Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Introduction Corn is one of the most important cereals used for poultry feeding due to its high starch, available energy and protein. Starch is an important source of energy in cereals and it is important to pay attention to its availability. In the cell wall of the endosperm, cereal grains, contain some of the structural carbohydrates that are soluble in the small intestine and have high molecular weight which may cause viscosity. Positive physical and chemical changes by steam conditioning, include starch gelatinization, denaturation of digestive enzyme inhibitor proteins, and cell wall breakage. Regardless of NSP content, some amounts of nutrients pass through the birds gut without being digested in corn-soy diets. Supplementing broiler diets with exogenous enzymes to degrade NSP has been a useful tool to release energy and nutrients, which can increase the value of low quality corn in poultry feeds and improve growth performance.
Materials and Methods This project was conducted at the Poultry Research Center of Ferdowsi University of Mashhad. A batch of corm grains was obtained from a commercial supplier and ground in a hammer mill to pass through screen sizes of 3.0 and 5.0 mm for starter (1-10 d) and grower (11-24 d) periods, respectively.
First trial: Apparent metabolizable energy (AME) and digestibility of corn crude protein (CP) and dry matter (DM) were determined in basal diets, which the corn was the sole source of ME and CP. Basal diets contained the conditioned (at temperatures of 55, 70 and 85 °C) or unconditioned corn and was or was not supplemented with enzyme (Rovabio®; Adisseo, France) at the amount of 0.5 g/kg diet. Total excreta collection was performed with 144 chicks in 8 treatments with 6 replicates and 3 birds each, during 7 to 9 days of age using battery cages. Excreta from each replicate cage were collected early in the morning and in the evening. After removing feathers, feed residues, and other contamination sources, excreta were air dried in an oven at 55 °C for 72 hours. Then the excreta were weighed and homogenized, then a sample of approximately 30% of the excreta was randomly separated and kept at -20 °C to further analysis. Then, dried excreta samples were ground in a micro-mill and submitted to the Animal Nutrition Lab. Feed and excreta dry matter, gross energy, and nitrogen contents were determined.
Second trial: In second trial, 576 d-old broilers from Ross 308 strain with initial BW of 43 g, were fed with mash diets containing conditioned corn which was or was not supplemented with enzyme, during starter (1-10 d) and grower (11-24 d) periods. The experiment was done based on a completely randomized design with 4 × 2 factorial arrangement of treatments evaluating four corn conditioning temperatures (unconditioned and conditioned at 55, 70 and 85 °C) and two enzyme (Rovabio®; Adisseo, France) levels (0 and 0.5 g/kg diet). chicks were assigned under eight treatments with 6 floor pen replicates. Each floor pen of 1.2 × 1 × 0.8 m (L × W × H) included 12 chicks (6 male and 6 female, equally for all replicates). Floor pens were covered with 1.5 Kg/m2 of wood shavings. Four of eight experimental diets were supplemented with 0.5 g/kg of a multiple enzyme complex composed of cellulases, 6400 unit; β-glucanase, 2000 unit and Xylanase 22000 unit per gram (Rovabio®; Adisseo, France). Corn-soy-based diets were formulated to meet the Ross 308 strain recommendations for major nutrients for starter and grower phases. Feed and water were supplied for ad libitum consumption throughout the trial with a tube feeder and two nipple drinkers in each floor pen. House temperature was initially set at 32 C on day one and was decreased linearly by 0.5 C per d to a temperature of 21 C. During the experiment, the lighting program consisted of 23L:1D. A completely randomized design with 4×2 factorial arrangement was used in the both trials. Main factors were included corn conditioning temperatures (unconditioned and conditioned at 55, 70 and 85 °C) and two enzyme levels (0 and 0.5 g/kg diet). The data were analyzed using generalized linear model (GLM) procedure, SAS software (9.4) and the differences between them was tested by Duncan's test (P ≤ 0.05).
Results and Discussion Conditioning and enzyme supplementation had no significant effects on AME and CP digestibility. Conditioning at 80 °C decreased DM digestibility of corn in comparison to control group (P<0.05). Enzyme had no significant effect on DM digestibility. In second trial, there were no significant differences in productive performance among treatments during 11-24 d and the accumulated period (1 to 24 d). Feed conversion ratio (FCR) of the birds fed diets containing conditioned corn at 85 °C was significantly more than that of 55 and 70 °C diets during 1-10 d; although it was not significantly different with control group. Neither corn conditioning nor enzyme supplementation had significant effect on carcass characteristics, and small intestine segments length and weight at 24 d. Enzyme supplementation significantly increased villi height and width at 24 d (P < 0.05). Corn conditioning significantly increased villi width and crypt depth but decreased villi width to crypt depth ratio. Bifidobacteria, E. coli and clostridia population in the ileal contents were not affected by corn conditioning and enzyme supplementation. Lactobacillus population was increased by enzyme addition and also by corn conditioning at temperature 70 °C in compare to control group. These results are in agreement with those reported by Gonza´lez-Alvarado et al. (2007) who reported no significant differences in growth performance of broiler chicks fed heat treated corn-based diets. However, negative effect of higher pelleting temperatures on the WG of birds fed corn-based diets have also been reported. They showed that pelleting a corn–soybean meal diet at 65 ◦C resulted in higher weight gain compared to the basal mash diet and diets pelleted at 75 and 85 ◦C.
Conclusion In general, corn conditioning and enzyme supplementation did not improve growth performance and nutrient utilization but improved gut histomorphology and microbial status.
 

Keywords


  1. Abdollahi, M. R., V. Ravindran, T. J. Wester, G. Ravindran, and D. V. Thomas. 2010. Influence of conditioning temperature on performance, nutrient utilization and digestive tract development of broilers fed on maize- and wheat –based diets. British Poultry Science, 51: 648-657.
  2. Amerah, A. M., C. Gilbert, P. H. Simmins, and V. Ravindran. 2010. Influence of feed processing on the efficacy of exogenous enzymes in broiler diets. World's Poultry Science Journal, 67: 29- 46.
  3. Amuzmehr, A., B. Dastar, M. Shamse-Shargh, and S. Zrehdaran. 2010. Effect of different levels of raw and processing rice bran on performance of broiler chicks. Animal Sciences, 1: 33-27. (In Persian)
  4. AOAC International. 2006. Official Methods of Analysis of AOAC International. 18th AOAC Int., Gaithersburg, MD.
  5. 2014. Nutrition Specifications Manual: Ross 308. Aviagen Ltd., Scotland, UK.
  6. Boorman, and J. McNab. 2002. Poultry Feedstuffs: Supply, Composition and Nutritive Value (Poultry Science Symposium) First Edition. CABI publishing Co., London, UK.
  7. Choct, M. 1999. Soluble non-starch polysaccharides affect net utilization of energy by chickens. Recent Advances in Animal Nutrition in Australia, 12: 31-35.
  8. Cowieson, A. J. 2005. Factors that affect the nutritional value of maize for broilers. Animal Feed Science and Technology, 119: 293-305.
  9. Cowieson, A. J., M. Hruby, and M. F. Isaksen. 2005. The effect of conditioning temperature and exogenous xylanase addition on the viscosity of wheat-based diets and the performance of broiler chickens. British Poultry Science, 46: 717-724.
  10. Fathi, M. H. 2010. Animal feeds Evaluation. Ayizh publishing Co., Tehran, Iran.
  11. Ghobadi, Z., and A. Karimi. 2012. Effect of feed processing and enzyme supplementation of wheat-based diets on performance of broiler chicks. Journal of Applied Animal Research, 40: 260-266.
  12. Gonza´lez-Alvarado, J. M., E. Jime´nez-Moreno, R. La´zaro, and G. G. Mateos. 2007. Effect of type of cereal, heat processing of the cereal and inclusion of fiber in the diet on productive performance and digestive traits of broilers. Poultry Science, 86: 1705–1715.
  13. Goodarzi Boroojeni, F., W. Vahjen, A. Mader, F. Knorr, I. Ruhnke, I. Rohe,A. Hafeez, C. Villodre, K. Manner, and J. Zentek. 2014. The effect of different thermal treatments and organic acid levels in feed on microbial composition and activity in gastrointestinal tract of broilers. Poultry Science, 93: 1440-1452.
  14. Iji, P. A., R. J. Hughes, M. Choct, and D. R. Tivey. 2001. Intestinal structure and function of broiler chickens on wheat-based diets supplemented with a microbial enzyme. Asian-Australasian Journal of Animal Science, 14: 54-60.
  15. Jime´nez-Moreno, E., J. M. Gonza´lez-Alvarado, R. La´zaro, and G. G. Mateos. 2009. Effect of type of cereal, heat processing of the cereal and fiber inclusion in the diet on gizzard pH and nutrient utilization in broilers at different ages. Poultry Science, 88: 1925–1933.
  16. Leeson, S., and J. D. Summers. 2001. Scott's nutrition of the chicken. 4th edition. Nottingham University Press, London, UK.
  17. Loar, R. E., K. G. S. Wamsley, A. Evans, J. S. Moritz, and A. Corzo, 2014. Effects of varying conditioning temperature and mixer-added fat on feed manufacture efficiency, 28-to-42-day broiler performance, early skeletal effect, and true amino acid digestibility. Journal of Applied Poultry Research, 23: 444-455.
  18. Massuquetto, A., J. Durao, V. G. Schramm, M. V. T. Netto, E. L. Krabbe, and A. Maiorka. 2017. Influence of feed form and conditioning time on pellet quality, performance and ileal nutrient digestibility in broilers. Journal of Applied Poultry Research, 27: 51-58.
  19. Mathison, G. W. 1996. Effects of processing on the utilization of grain by cattle. Animal Feed Science and Technology, 58: 113-125.
  20. Medel, P., M. A., Latorre, C. De Blas, R. Lazaro, and G. G. Mateos. 2004. Heat processing of cereals in mash or pellet diets for young pigs. Animal Feed Science and Technology, 113: 127-140.
  21. Omede, A. A., and P. A. Iji. 2018. Response of broiler chickens to processed soy protein product when offered at different inclusion levels in mash or crumbled prestarter diets. Journal of Applied Poultry Research, 27: 159-171.
  22. Pickford, J. R. 1992. Effect of processing on the stability of heat labile nutrients in animal feeds. Pages 177–192 in Recent Advances in Animal Nutrition. Cole D. J., Haresing W. Ed. Butterworth-Heinemann Ltd. UK.
  23. Pirgozliev, V. R., C. L. Birch, S. P. Rose, P. S. Kettlewell, and M. R. Bedford. 2003. Chemical composition and the nutritive quality of different wheat cultivars for broiler chickens. British Poultry Science, 44: 464–475.
  24. Ravindran, V., Z. V. Tilman, P. C. H. Morel, G. Ravindran, and G. D. Coles. 2007. Influence of β-glucanase supplementation on the metabolisable energy and ileal nutrient digestibility of normal starch and waxy barleys for broiler chickens. Animal Feed Science and Technology, 134: 45-55.
  25. SAS Institute Inc. 2004. User’s guide, version 9.1. Cary, NC: SAS Institute Inc.
  26. Seena, S., K. R. Sridhar, A. B. Arunb, and C. C. Young. 2006. Effect of roasting and pressure-cooking on nutritional and protein quality of seeds of mangrove legume Canavalia Cathartica from southwest coast of India. Journal of Food Comparative Analysis, 19: 284-293.
  27. Silversides, F. G., and M. R. Bedford. 1999. Effect of pelleting temperature on the recovery and efficacy of a xylanase enzyme in wheat-based diets. Poultry Science, 78: 1184 -1190.
  28. Skoch, E. R., K. C. Behnke, C. W. Deyoe, and S. F. Binder. 1981. The effect of steam- conditioning rate on the pelleting process. Animal Feed Science and Technology, 6: 83-90.
  29. Svihus, B., A. K. Uhlen, and O. M. Harstad. 2005. Effect of starch granule structure, associated components and processing on nutritive value of cereal starch. Animal Feed Science Technology, 122: 303-320.
  30. Sylvanus, D. P. O, P. K. Malumba, Y. Beckers, and B. Francois. 2015. Impact of drying and heat treatment on the feeding value of corn. A review. Biotechnology, Agronomy, Society and Environment, 19: 301-312.
  31. Tiemouri, H., H. Zarghi, and A. Golian. 2017. Effect of enzyme supplementation on metabolic energy, digestibility of dry matter and crude protein in barley in broiler chickens. Iranian Journal of Animal Science Research, 10: 513-523. (In Persian)
  32. Wiseman, J. 2000. Correlation between physical measurements and dietary energy values of wheat for poultry and pigs. Animal Feed Science Technology, 84: 1-11.
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Volume 13, Issue 3 - Serial Number 47
September 2021
Pages 369-388
  • Receive Date: 18 October 2019
  • Revise Date: 26 November 2019
  • Accept Date: 06 January 2020
  • First Publish Date: 27 November 2020