Effect of Hyssopus Officinalis, Aspirin and Virginiamycin on Performance, Blood Metabolites, Carcass Parameters and Ileum Microbial Population of Broiler Chickens under Cold Stress

Document Type : Poultry Nutrition

Authors

1 Department of Animal Sciences, Ilam University, Ilam, Iran

2 Animal Science Research Department, Ilam Agricultural and Natural Resources Research and Education Center, AREEO, Ilam, Iran.

3 Department of Animal Science, Ilam University, Ilam, Iran

Abstract

Introduction[1] During decades, growth rate of broiler chickens was increased more than 4 times whereas growth rate of cardiovascular and respiratory tracts did not increase as muscle growth rate. Imbalance between oxygen consuming orangs and oxygen supplying organs led to increase metabolic disorders like ascites in broiler chickens. Low oxygen supplementation is the important factor in ascites syndrome by reducing arterial oxygen pressure and increasing pulmonary artery pressure. Increased pulmonary artery pressure led to hypertrophy of cardiac right ventricle and finally ascites incidence in broiler chickens during cold environment condition.
Antibiotics as growth promoters in poultry feed are posing serious health risks to human health, because of their residual effects in poultry meat and eggs, as well as result pathogens develop resistance to antibiotics. Currently, poultry scientists are challenged to find out alternatives to antibiotic growth promoters with no side effects for poultry that could be more or as effective against harmful microorganisms in the gastrointestinal tract and to stimulate the growth by increasing the efficiency of feed utilization and to enhance the immunity. Regarding to this subject, supplementing the dietary herbs or plant extracts would stimulate the productive performance of poultry. One of the most frequently consumed herbal remedies available today is the hyssop preparations prepared from Hyssopus officinalis (L) which is gaining increased importance as a minty flavor, condiment and spices in food industries as well. The GC and GC-MS analysis of the essential oil led to the identification of 21 compounds representing 95.6% of the oil, comprising seven monoterpene hydrocarbons (32.3%), five oxygenated monoterpenes (60.5%), one phenol (0.2%) and six sesquiterpene hydrocarbons (0.35%). The major constituents of the camphorous predominant monoterpenes of the oil were pinocamphone (49.1%) >β-pinene (18.4%). According to the results of the studies, hyssop extract showed much weaker antioxidant activity as compared to the rosemary, sage, and thyme extracts in different methods of antioxidant evaluations. The aim of this study was comparing the effects of Hyssopus Officinalis powder, Virginiamycin antibiotic and Aspirin on performance, blood metabolites, carcass parameters and ileum microbial population of broiler chickens which subjected to cold stress were studied.
Materials and Methods In a completely randomized design, a total of 500 male Ross-308 broiler chickens were allocated to 5 treatments with 5 replicates and 20 birds in each. Dietary treatment consisted of: 1) control, 2 and 3) basal diet plus 300 g/ton Virginiamycin or Aspirin respectively and 4 and 5) basal diet containing 0.5 or one percent Hyssopus, respectively. The diets were formulated to meet the requirements of broilers as established by the Ross 308 broilers feeding guide in starter (1-11 d), grower (12-25 d) and finisher (26-42 d). The birds were kept under conventional conditions for vaccination, temperature, ventilation, and lighting based on Ross catalogue recommendations. Standard management practices of commercial broiler production were applied. The broiler diets were formulated based on standardized ileal digestible amino acids and other requirements were obtained from Ross catalogue recommendations. Broiler chicken performance (feed intake, body weight gain, feed conversion efficiency, total and ascytic mortality and European production efficiency factor), erythrocyte osmotic fragility (EOF), blood cell count and blood metabolites including triglyceride, total cholesterol, HDL and LDL Cholesterol were measured at the 21 and 42 d of age. Carcass and abdominal fat percentage were calculated. Finally population of Lactobacillus and Escherichia coli of ileum were detected.
Results and Discussion As compared to control, inclusion of 1 percent Hyssopus increased feed intake (P < 0.05). Addition of Hyssopus and antibiotic increased weight gain, EPEF and decreased FCR compared to control and aspirin groups. Addition of one percent Hyssopus decreased red blood cell count, erythrocyte osmotic fragility (EOF) and heterophil percentage at 21 and 42 d of age as compared to control (P < 0.05). Escherichia coli population was reduced by antibiotic and one percent Hyssopus inclusion into broiler’ diet. Dietary treatments had no effect on WBC count, lymphocyte percentage, heterophil to lymphocyte ratio, serum total cholesterol, HDL- and LDL-cholesterol and urea concentration of broiler chickens at 21 and 42 d of age, carcass, and thigh meat and abdominal fat percentages at 42 d of age (P > 0.05) whereas broiler chickens fed diet containing 0.5 and 1 % hyssopus powder had higher breast meat percentage.
Conclusion In conclusion addition of aspirin or Hyssopus powder improved broiler chicken’s performance and decreased mortality due to ascites and heterophile to lymphocyte ratio in cold condition. Virginiamycin improved broiler chickens body weight gain in cold condition but due to probable drug resistance, it may suggest that Hyssopus supplementation in broiler diet as a good replacement for antibiotic in cold stress.
 
 

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  1. Aarif, O., and P. S. Mahapatra. 2013. The effect of cold stress on biochemical and hematological parameters in broad breasted white turkeys. Wyno Journal of Biological Sciences, 1(4):20-23.
  2. Abbasi, O., and Sh. Rahimi. 2016. Effect of Medieinal Herbs on Poultry under Heat stress. MSc Thesis, Tarbiat Modarred University.
  3. Ao, X., J .S. Yoo, T. X. Zhou, J. P. Wang, Q.W. Meng, L. Yan, J. H. Cho, and I.H. Kim. 2011. Effects of fermented garlic powder supplementation on growth performance, blood profiles and breast meat quality in broilers. Livestock Science, 141(1):85-89.
  4. 2014. Ross 308 Broiler Nutrition Specification Managementguide.
  5. Bagherzadeh, K. F., S. Omidikia, H. R. Mirzaei, and M. Mehri. 2015. Effects of Salvia mirzayanii leaf powder on performance and cecal microbial population of broilers. Journal of Animal Production (Journal of Agriculture), 2(16):103-111. (In Persian)
  6. Brenes, A., and E. Roura. 2010. Essential oils in poultry nutrition: Main effects and modes of action. Animal Feed Science and Technology, 158(1-2): 1-14.
  7. Chowdhury, S., G. P. Mandal, A.K. Patra, P. Kumar, I. Samanta, S. Pradhan, and A.K. Samanta. 2018. Different essential oils in diets of broiler chickens: 2. Gut microbes and morphology, immune response, and some blood profile and antioxidant enzymes. Animal Feed Science and Technology, 236: 39-47.
  8. Demir, E., K. Kilinc, Y. Yildirim, F. Dincer, and H. Eseceli. 2008. Comparative effects of mint, sage, thyme and flavomycin in wheat-based broiler diets. Archiva Zootechnica, 11(3):54-63.
  9. Dovizio, M., S. Tacconelli, C. Sostres, E. Ricciotti, and P. Patrignani. 2012. Mechanistic and pharmacological issues of aspirin as an anticancer agent. Pharmaceuticals, 5(12):1346-1371.
  10. Fathi, M., K. Nazeradl, Y. E. Nezhad, H. A. Shahryar, M. Daneshyar, and T. Tanha. 2011. Antioxidant enzyme activities characterization in pulmonary hypertension syndrome (PHS) in broilers. Research Journal of Biological Sciences, 6(3):118-123.
  11. Fathi, M., M. Haydari, and T. Tanha. 2016. Influence of dietary aspirin on growth performance, antioxidant status, and mortality due to ascites in broiler chickens. Poultry Science Journal, 4(2):139-146.
  12. Fathiazad, F., and S. Hamedeyazdan. 2011. A review on Hyssopus officinalis Composition and biological activities. African Journal of Pharmacy and Pharmacology, 5(17):1959-1966.
  13. Havenstein, G.B., P.R. Ferket, and M.A. Qureshi. 2003. Growth, livability, and feed conversion of 1957 versus 2001 broilers when fed representative 1957 and 2001 broiler diets. Poultry Science, 82(10):1500-1508.
  14. Hoehler, D., A. Lemme, V. Ravindran, W. L. Bryden, and H. S. Rostagno. 2006. Feed formulation in broiler chickens based on standardized ileal amino acid digestibility. Avances en Nutrición Acuicola, 1:78-91.
  15. Huff, G. R., W. E. Huff, N. C. Rath, F. Solis De Los Santos, M. B. Farnell, and A. M. Donoghue. 2007. Influence of hen age on the response of turkey poults to cold stress, Escherichia coli challenge, and treatment with a yeast extract antibiotic alternative. Poultry science, 86(4): 636-642.
  16. Kadowitz, P.J., H.L. Lippton, D.B. McNamara, E.W. Spannhake, and A.L. Hyman. 1982. Action and metabolism of prostaglandins in the pulmonary circulation. Advances in prostaglandin, thromboxane, and leukotriene research, 10:333.
  17. Koochaksaraie, R. R., M. Irani, and S. Gharavysi. 2011. The effects of cinnamon powder feeding on some blood metabolites in broiler chicks. Brazilian journal of poultry science, 13(3): 197-202.
  18. Luger, D., D. Shinder, D. Wolfenson, and S. Yahav. 2003. Erythropoiesis regulation during the development of ascites syndrome in broiler chickens: A possible role of corticosterone. Journal of Animal Science, 81(3):784-790.
  19. Mehala, C., and M. Moorthy. 2008. Production performance of broilers fed with Aloe vera and Curcuma longa (Turmeric). International Journal Poultry Science, 7(9):852-856.
  20. Mmereole, F. U. C. 2011. Evaluation of the dietary inclusion of aloe vera as an alternative to antibiotic growth promoter in broiler production. Pakistan Journal of Nutrition, 10(1):1-5.
  21. Mohamadamini, M., F. Shariatmadari, and S.A. Hosseini. 2015. The effects of turmeric, thyme and cinnamon on parameters related to ascites syndrome in Arian broilers. Iranian Journal of Medicinal and Aromatic Plants, 31(3):436-445.
  22. Moro, M. H., G. W. Beran, L. J. Hoffman, and R.W. Griffith. 1998. Effects of cold stress on the antimicrobial drug resistance of Escherichia coli of the intestinal flora of swine. Letters in applied microbiology, 27(5):251-254.
  23. Ogunwande, I. A., G. Flamini, P. L. Cioni, O. Omikorede, R. A. Azeez, A. A. Ayodele, and Y. O. Kamil. 2010. Aromatic plants growing in Nigeria: essential oil constituents of Cassia alata (Linn.) Roxb. and Helianthus annuus L. Records of Natural Products, 4(4):211-217.
  24. Pakdel, A., J. A. Van Arendonk, A. L. Vereijken, and H. Bovenhuis. 2002. Direct and maternal genetic effects for ascites-related traits in broilers. Poultry science, 81(9):1273-1279.
  25. Rafiee, F., M. Mazhari, M. Ghoreishi, and O. Esmaeilipour. 2016. Effect of lemon verbena powder and vitamin C on performance and immunity of heat‐stressed broilers. Journal of Animal Physiology and Animal Nutrition, 100(5):807-812.
  26. Rahimi, S., Z. Z. Teymouri, T. M. Karimi, R. Omidbaigi, and H. Rokni. 2011. Effect of the three herbal extracts on growth performance, immune system, blood factors and intestinal selected bacterial population in broiler chickens. Journal of Agricultural Science and Technology, 13:527-539.
  27. Rajani, J., M. K. Torshizi, and S. Rahimi. 2011. Control of ascites mortality and improved performance and meat shelf-life in broilers using feed adjuncts with presumed antioxidant activity. Animal Feed Science and Technology, 170(3-4):239-245.
  28. Rotolo, L., F. Gai, S. Nicola, I. Zoccarato, A. Brugiapaglia, and L. Gasco. 2013. Dietary supplementation of oregano and sage dried leaves on performances and meat quality of rabbits. Journal of Integrative Agriculture, 12(11):1937-1945.
  29. SAS Institute. 2004. SAS User’s Guide. Version 8 ed. SAS Inst. Inc., Cary, NC.
  30. Shirzadi, H., F. Shariatmadari, and M.A. Karimi-torshizi. 2014. Effect of Rhuscoriaria and Prosopis farcta on microbial population and ascytes control in broiler chickens reared under cold stress. PhD Thesis, Tarbiat Modares University.
  31. Shokri, A. N., M. Akbari-Gharaei, S. Varmaghani, K. Taherpour, A. Khatibjoo, and M. Soltani. 2018. Effect of Lemon verbena, Artichoke and Common sage on response of broilers chickens under cold stress. PhD Thesis, Ilam University.
  32. Swayne, D. E., 1998. Laboratory manual for the isolation and identification of avian pathogens. American Association of Avian Pathologists, University of Pennsylvania.
  33. Tajodini, M., F. Samadi, S. R. Hashemi, S. Hassani, and M. Shams-Shargh. 2014. Effect of different levels of Artichoke (Cynara scolymus) powder on the performance and immune response of broiler chickens. International Journal of Agri Science, 4(1):66-73.
  34. Talpur, N., B. Echard, C. Ingram, D. Bagchi, and H. Preuss. 2005. Effects of a novel formulation of essential oils on glucose–insulin metabolism in diabetic and hypertensive rats: a pilot study. Diabetes, Obesity and Metabolism, 7(2): 193-199.
  35. Tsiouris, V., I. Georgopoulou, C. Batzios, N. Pappaioannou, R. Ducatelle, and P. Fortomaris. 2015. The effect of cold stress on the pathogenesis of necrotic enteritis in broiler chicks. Avian Pathology, 44(6): 430-435.
  36. Varmaghany, S., M. A. K. Torshizi, S. Rahimi, H. Lotfollahian, and M. Hassanzadeh. 2015. The effects of increasing levels of dietary garlic bulb on growth performance, systolic blood pressure, hematology, and ascites syndrome in broiler chickens. Poultry Science, 94:1812-1820.
  37. Wideman, R. F., D. D. Rhoads, G. F. Erf, and N. B. Anthony. 2013. Pulmonary arterial hypertension (ascites syndrome) in broilers: a review. Poultry Science, 92(1):64-83.

 

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  • Receive Date: 30 December 2019
  • Revise Date: 15 June 2020
  • Accept Date: 23 June 2020
  • First Publish Date: 27 November 2020