Effect of Use of Mixture of Herbal Extracts on Performance, Carcass Characteristics, Blood Serum Metabolites and Enzyme Activity of Broiler Chickens

Document Type : Poultry Nutrition


1 gorgan

2 birjand

3 Department of Animal Sciences, University of Birjand, Birjand, Iran


Introduction In view of rising concerns on the extensive use of antibiotics in animal production, there is an increasing interest for developing alternative disease control strategy to enhance animal health and to reduce the use of antimicrobials. One promising new possibility to achieve this goal is the use of natural foods and herbal products to enhance feed efficiency, gut health, and innate immunity. In clinical medicine, plant-derived products are increasingly being used as feed supplements to enhance immunity to diseases and cancers. Among these products, the dietary effects of the mixture of three plant derived phytochemicals, carvacrol, cinnamaldehyde, and Capsicum oleoresin as anti-bacterial and anti-fungal agents have been reported. Carvacrol is a component of numerous aromatic plants, such as Origanum vulgare, thyme, and wild bergamot. The anti-microbial functions of these herbs are associated with carvacrol since carvacrol vapour has been shown to inhibit Salmonella growth in chickens. Cinnamaldehyde is a constituent of cinnamon and widely applied as flavoring. It has been proven to have strong anti-bacterial activity against Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, methicillin-resistant Staphylococcus aureus (MRSA), Klebsiella pneumoniae, Salmonella sp., and Vibrio parahemolyticus. Capsicum oleoresin, prepared by organic extraction of pepper fruits, contains anti-bacterial activity. It contains the pungent principles, capsicin which has effects on the resistance to Salmonella enteritidis infection by altering pH and histological changes.
Materials and Methods In this study, 160 Ross 308 male broiler chicks were allocated in a completely randomized design with 5 treatments, 4 replicates of 8 chicks in each replicate for 42 days. Treatments contained 1 level of oxytetracycline antibiotic and 3 levels of extract supplementation (50, 100 and 200 mg/kg of feed). The rearing and management conditions were equal for all groups. Experimental diets were formulated to meet the nutrients requirements of the Ross broiler chicks. Chicks had full access to feed and water during the experimental period. Performance parameter such as body weight gain, feed intake and feed conversion ratio were recorded at 1-10, 11-24 and 25-42 d of age for each replicate. At the end of the experiment, two birds of each replicate were slaughtered and blood was collected to determine hematological parameters, and carcass characteristics were analyzed.
Results and Discussion Based on the results, in 1-10 d period supplementing the broiler diet with 100 mg/kg herbal extracts decreased significantly feed intake (P


1- Agostini, P. S, and D. Sola-Oriol. 2012. Role of infeed clove supplementation on growth performance, intestinal microbiology, and morphology in broiler chicken. Livestock Science, 147: 113-118.
2- Al-Kassie, G. A. M, and M. A. M. Al-Nasrawi. 2011. The effects of using hot red pepper a diet supplement on some performance traits in broiler. Pakistan Journal of Nutrition, 10: 842-845.
3- AL-Kassie, G. A. M. 2009. Influence of two plant extracts derved from thyme and cinamon on broiler performance. Pakistan Veterinary Journal, 29(4): 169-173.
4- Alp, M., M. Midilli., N. Kocabagl., H. Yilmaz., N. Turan., A. Gargil, and N. Acar. 2012. The effects of dietary oregano essential oil on live performance, carcass yield serum immunoglobulin g level, and oocyst count in broilers. Journal of Applied Poultry Research, 21: 630-636.
5- Atapattu, N. S. B. M, and U. D. Belpagodagamage. 2010. Effect of dietary chili powder growth performance and serum cholesterol contents of broiler chicken. Tropical Agricultural Research and Extension, 13: 106-109.
6- Azaz, D., F. Demirci., F. Satil., U. Kürkcüog, and K. H. Baser. 2002. Antimicrobial activity of some Satureja oils. Zeitschrift für Naturforschung, 57: 817-821.
7- Behrooz Lak, M. A., A. Hassan Abadi., H. Nasiri Moghadam, and H. Kermanshahi. 2014. Effect of different levels of cinnamon powder, with antibiotic and probiotic on performance and carcass characteristics of broiler chickens. Research on Animal Production, 5: 25-35. (In Persian).
8- Botsoglou, N., E. Christaki., P. F. Paner., I. Giannenas., G. Papageorgiou, and A. Spais. 2004. The effect of a mixture of herbal essential oils or a-tocopheryl acetate on performance parameters and oxidation of body lipid in broiler. South African Journal of Animal Science, 34: 52-61.
9- Botsoglou, N., P. Florou-Paner., E. Christaki., D. G. Fletouris, and A. B. Spais. 2002. Effect of dietary oregano essential oil on performance of chickens and on iron induced lipid oxidation of breast, thigh and abdominal fat tissues. British Poultry Science Journal, 43: 223-230.
10- Bozkurt, M., k. Kucukyilmaz., A. U. Catli, and M. Cinar. 2008. Growth performance and slaughter characteristics of broiler chickens fed antibiotic, mannan oligosaccharide and dextran oligosaccharide supplemented diets. International Journal of Poultry Science, 7(10): 969-977.
11- Bravo, D., P. Utterback, and C. M. Parsons. 2011. Evaluation of a mixture of carvacrol, cinnamaldehyde, and capsicum oleoresin for improving growth performance and metabolizable energy in broiler chicks fed corn and soybean meal. Journal of Applied Poultry Research, 20: 115–120.
12- Brenes, A, and E. Roura. 2010. Essential oils in poultry nutrition: Main effects and modes of action. Animal Feed Science and Technology, 158: 1–14.
13- Burt, S. A., M. J. Fledderman., H. P. Haagsman., F. van Knapen, and E. J. Veldhuizen. 2007. Inhibition of Salmonella enterica serotype Enteritidis on agar and raw chicken by carvacrol vapour. International Journal of Food Microbiology, 119(3): 346-350.
14- Burt, S. A., R. Vlielander., H. P. Haagsman, and E. J. Veldhuizen. 2005. Increase in activity of essential oil components carvacrol and thymol against Escherichia coli O157:H7 by addition of food stabilizers. Journal of Food Protection, 68(5): 919-926.
15- Case, G. L., L. He., H. Mo, and C. E. Elson. 1995. Induction of geranyl pyrophosphate pyrophosphatase activity by cholesterol-suppressive isoprenoids. Lipids, 30: 357-359.
16- Chang, S. T., P. F. Chen, and S. C. Chang. 2001. Antibacterial activity of leaf essential oils and their constituents from Cinnamomum osmophloeum. Journal of Ethnopharmacology, 77(1): 123-127.
17- Ciftci, M., U. G. Simsek., A. Yuce., O. Yilmaz, and B. Dalkilic. 2010. Effects of dietary antibiotic and Cinnamon oil supplementation on antioxidant enzyme activities, cholesterol levels and fatty acid compositions of serum and meat in broiler chickens. Journal of Acta Veterinaria Brno, 79: 33-40.
18- Dashti-Rahmatabadi, M., A. Vahidi Merjardi., A. Pilavaran, and F. Farzan. 2009. Antinociceptive Effect of Cinnamon Extract on Formalin Induced Pain in Rat. The Journal of Shahid Sadoughi University of Medical Sciences, 17: 190-199. (In Persian).
19- De Vincenzi, M., A. Stammati., A. De Vincenzi, and M. Silano. 2004. Constituents of aromatic plants: carvacrol. Fitoterapia, 75(7-8): 801-804.
20- Dorman, H. J. D, and S. G. Deans. 2000. Antimicrobial agent from plants: antimicrobial activity of plant volatile oils. Journal of Applied Microbiology, 88: 308-316.
21- Eldeeb, M., M. A. Metwally, and A. E. Galal. 2006. The impact of botanical extract, capsicum (capsicum frutescence l), oil supplementation and their interactions on the productive performance of broiler chicks. World's Poultry Science, 243-247.
22- Eltazi, S. M. A. 2014. Effect of using cinnamon powder as natural feed additive on performance and carcass quality of broiler chicks. International Journal of Innovations and Research, 2 (3):1-8.
23- Esmaeili, H., R. Vakili., N. Afzali., A. Amiri., H. Naeimipoor, and M. Nasimi. 2010. The effect of the use of cinnamon powder in ration on performance of broiler chickens. 4th Congress of Animal Science. College of Agriculture and Natural Resources, Karaj, Iran. (In Persian).
24- Esteva-Garcia, E, and S. Mack. 2000. The effect of DL-methionine and betaine on growth. Performance and carcass characteristics in broilers. Animal Feed Science and Technology, 87: 151-159.
25- Galib, A. M., Y. Butris., B. Saba, and J. Ajeena. 2012. The potency of feed supplemented mixture of hot red pepper and black pepper on the performance and some hematological blood traits in broiler diet. International Journal of Advanced Biological Research, 2: 53-57.
26- Gill, C. 2001. Safe and sustainable feed ingredients. Feed International, 22: 40-45.
27- Hammer, K. A., C. F. Carson, and T. V. Rilley. 2002. Antimicrobial activity of essential oils. Journal of Applied Microbiology, 86: 985- 990.
28- Hashemi, S. R., I. Zulkifli., H. Davoodic., Z. Zunita, and M. Ebrahimi. 2012. Growth performance, intestinal microflora, plasma fatty acid profile in broiler chickens fed herbal plant (Euphorbia hirta) and mix of acidifiers. Animal Feed Science and Technology, 178: 167– 174.
29- Hashemipour, H., H. Kermanshahi., A. Golian, and T. Veldkamp. 2013. Effect of thymol and Carvacrol feed supplementation on performance, antioxidant enzyme activities, fatty acid composition, digestive enzyme activities, and immune response in broiler chickens. Poultry Science, 92: 2059-2069.
30- Hernandez, F., J. Madrid., V. Garcia., J. Orengo, and M. D. Megias. 2004. Influence of two plant extract on broiler performance, digestibility, and digestive organ size. Poultry Science, 83: 169-174.
31- Hernandez, F., J. Madrid., V. Garcia., J. Orengo, and M. D. Megias. 2004. Influence of Two plant extracts on high fat diet. Nutrition Journal, 116: 1272–1278.
32- Heydari, A., A. Nobakht, and A. R. Safdamehr. 2010. Evaluation the effects of medical herbs nettle, Pennyroyal and Ziziphora and their mixture on blood biochemical parameters and immune of broiler chickens. Pages 214-217 in Proc. 4th Congress of Animal Science. College of Agriculture and Natural Resources, Karaj, Iran. (In Persian).
33- Jamroz, D, and C. Kamel. 2002. Plant extracts enhance broiler performance. In non ruminant nutrition: Antimicrobial agents and plant extracts on immunity. Health and Performance, 80(1): 41-46.
34- Jamroz, D, and C. Kamel. 2002. Plant extracts on immunity. Health and Performance, 80: 41-46.
35- Jamroz, D., A. Wiliczkiewicz., T. Wertelecki., J. Orda, and J. Skorupinska. 2005. Use of active substances of plant origin in chicken diets based on maize and locally grown cereals. British Poultry Science, 46(4): 485-493.
36- Jamroz, D., J. Orda., C. Kamel., A. Wiliczkiewicz., T. Wertelecki, and I. Skorupinska. 2003. The influence of phytogenic extracts on performance, nutrient digestibility, carcass characteristics, and gut microbial status in broiler chickens. Journal of Animal and Feed Sciences, 12: 583–596.
37- Jamroz, D., T. J. Wertlecki., J. Orda., A. Wiliczkiewicz, and J. Skorupinska. 2003. Influence of phytogenic extracts on gut microbial status in chickens. Page 176 in Proc. 14th European Symposium on Poultry Nutrition, August, Lillehammer, Norwa.
38- Kamel, C. 2001. Tracing modes of action and the roles of plant extracts in nonruminants. Pages 135-150 in Recent Advances in Animal Nutrition. P. C. Garnsoworthy, and J. Wiseman, ed. Nottingham University Press, Nottingham.
39- Khaligh, F., G. Sadeghi., A. Karimi, and A. Vaziry. 2011. Evaluation of different medicinal plants blends in diets for broiler chickens. Journal of Medicinal Plants Research, 5: 1971-1977.
40- Kırkpınar, F., H. Bora Unlu, and G. Ozdemir. 2011. Effects of oregano and garlic essential oils on performance, carcass, organ and blood characteristics and intestinal micro flora of broilers. Livestock Science, 137: 219-225.
41- Koochaksaraie, R. R, and S. Gharavysi. 2011. The effect of cinnamon powder on some blood metabolites in broiler chicks. Brazilian Journal of Poultry Science, 3(13): 197-201.
42- Langhout, P. 2000. New additives for broiler chickens. World's Poultry Science Journal, 16: 22-27.
43- Lee, K. W., H. Everts., H. J. Kappert., M. Frehner., R. Losa, and A. C. Beynen. 2003. Effects of dietary essential oil components on growth performance, digestive enzymes and lipid metabolism in female broiler chickens. British Poultry Science, 44: 450–457.
44- Lee, S. H., H. S. Lillehoj., Y. H. Hong., S. I. Jang., E. P. Lillehoj., C. Ionescu., L. Mazuranok, and D. Bravo. 2010. In vitro effects of plant and mushroom extracts on immunological function of chicken lymphocytes and macrophages. British Poultry Science, 51(2): 213-221.
45- Mandegar, S. 2009. Poultry meat production without use of antibiotics. Journal of Veterinary Medicine, (18): 4-6. (in Persian).
46- Mansoub, N. N. 2011. Comparative effect of using zizaphora, garlic and probiotic on performance and serum composition of broiler chickens. Annals of Biological Research, 2: 373-378.
47- Mathew, S, and T. E. Abraham. 2006. Studies on the antioxidant activities of cinnamon (Cinnamomum verum) bark extracts, through various in vitro models. Journal of Food Chemistry, 94: 520-528.
48- McElroy, A. P., J. G. Manning., L. A. Jaeger., M. Taub., J. D. Williams, and B. M. Hargis. 1994. Effect of prolonged administration of dietary capsaicin on broiler growth and Salmonella enteritidis susceptibility. Avian Diseases, 38(2): 329-333.
49- Modaresi, M., M. Messripour, and R. Rajaei. 2009. The effect of cinnamone (bark) extract on male reproductive physiology in mice. Armaghane-danesh Journal, 53: 67-71. (In Persian).
50- Moorthy, M., S. Ravi., M. Ravikumar., K. Viswanathan, and S. C. Edwin. 2009. Ginger, Pepper and curry leaf powder as feed additives in broiler diet. International Journal of Poultry Science, 8(8): 779-782.
51- Nobakht, A, and H. Shahriyar aghdam. 2010. Effects of mixture of medicinal herbs MalvaSylvestris, alhagi and Mint on performance carcass quality and blood metabolites of broiler chickens. Journal of Animal Science, 3(3): 51-63. (In Persian).
52- Nobakht, A, and Y. Mehmannavaz. 2010. Effects of the use of the herb Mint, thyme and oregano on performance, egg quality and blood parameter of laying hens. Journal of Animal Science, 41: 129-136. (In Persian).
53- Nobakht, A., J. Norany, and A. R. Safamher. 2011. The effects of different amounts of Mentha pulegium L. (Pennyroyal) on performance, carcass traits, hematological and blood biochemical parameters of broilers. Journal of Medicinal Plants Research, 5: 3763-3768.
54- Nobakht, A., M. Rahimzadeh, and Y. Mehmannavaz. 2010. Effects of different levels of nettle herb, Pennyroyal and thymus in the early stages of growth. -On performance and carcass meat quality of chickens. Pages 40-43 in Proc. 4th Congress of Animal Science. College of Agriculture and Natural Resources, Karaj, Iran. (In Persian).
55- Park, B. 2008. Effect of dietary cinnamon powder on savor and Quality of chicken meat in broiler chickens. Journal of Korean Society of Food Science Nutrition, 37: 618-624.
56- Park, S. O., C. M. Ryo., B. S. Park, and J. Hwangbo. 2013. The meat quality and growth performance in broiler chickens fed diet with cinnamon powder. Journal of Environmental Biology, 34: 127-133.
57- Platel, K, and K. Srinivasan. 2001. A study of the digestive stimulant action of select spices in experimental rats. Journal of Food Science and Technology, 38: 358–361.
58- Platel, K., A. Rao., G. Saraswathi, and K. Srinivasan. 2002. Digestive stimulant action of three Indian spice mixes in experimental rats. Nahrung, 46: 394–398.
59- Platel, K., K. Srinivasan. 2003. Digestive stimulant action of spices: A myth or reality. Indian Journal of Medical Research, 119: 167-179.
60- Rehman, S., F. R. Durrani., N. Chand., R. U. Khan, and F. Rehman. 2011. Comparative efficacy of different schedules of administration of medicinal plants infusion on hematology and serum biochemistry of broiler chicks. Research Opinion in Animal and Veterinary Sciences, 1: 8-14.
61- Rose, J. 1997. Herbal and nutritional support for the Immune System. Journal of Clinical nutrition insights, 6:1-4.
62- Sadeghi. G. H., A. Karimi., S. H. Padidar Jahromi., T. Azizi., A. daneshmand. 2012. Effect of cinnamon thyme and turmeric infusions on the performance and immune response in of 1- to 21- day-old male broilers. Brazilian Journal of Poultry Science, 14: 15-20.
63- Sambaiah, K, and Μ. Ν. Satyanarayana. 1982. Influence of red pepper and capsaicin on body compositionand lipogenesis in rats. Journal of Biosciences, (4): 425-430.
64- Sarica, S., A. Ciftci., E. Demir., K. Kilinc, and Y. Yildirim. 2005. Use of antibiotic growth promoter and two herbal natural feed additives with and without exogenous enzymes in wheat based broiler diets. South African Journal of Animal Science, 35: 61-72.
65- SAS Institute. 2004. User’s Guids Version 9.1: Statistics. SAS Institute, Cary N.C.
66- Shalaei, M, and S. M. Hosseini. 2014. Effect of use purslane and milk thistle medical plants in the diet on enzymes activity, blood metabolites and egg characteristics of laying hens. Iranian Journal of Animal Science, 106(1): 91-102. (In Persian).
67- Suriya, R., I. Zulkifli, and A. R. Alimony. 2012. The effect of dietary inclusion of herbs as growth promoter in broiler chickens. Journal of Animal and Veterinary Advance, 11(3): 346-350.
68- Tellez, G. I., L. Jaeger., C. E. Dean., D. E. Corrier., J. R. DeLoach., J. D. Williams, and B. M. Hargis. 1993. Effect of prolonged administration of dietary capsaicin on Salmonella enteritidis infection in leghorn chicks. Avian Diseases, 37(1): 143-148.
69- Ultee, A., E. P. W. Kets, and E. J. Smid. 1999. Mechanisms of action of carvacrol on the food borne pathogen Bacillus cereus. Applied and Environmental Microbiology, 65: 4606-4610
70- Veldhuizen, E. J., J. L. Tjeerdsma-van Bokhoven., C. Zweijtzer., S. A. Burt, and H. P. Haagsman. 2006. Structural requirements for the antimicrobial activity of carvacrol. Journal of Agricultural and Food Chemistry, 54(5): 1874-1879.
71- Williams, P, and R. Losa. 2001. The use of essential oils and their compounds in poultry nutrition. Worlds Poultry Science Journal, 17: 14-15.
72- Wu, X., G. R. Beecher, and J. M. Holden. 2004. Lipopholic and hydrophilic antioxidant capacities of common food in the United States. Journal of Agricultural and Food Chemistry, 52: 4026-4037.
73- Zargari, A. 2002. Medicinal Herbs. Volume II, University of Tehran Press, Tehran, Iran. (In Persian).