Scrutiny and Amplification of the Myostatin gene pro-motor region and sequential analysis in Khuzestan province local Cattles

Document Type : Genetics & breeding

Authors

• Zeinab Mohammadi
• Hedayatollah roshanfekr ¹
• Mohammad Taghi Beigi Nasiri ²
• Hamid Rajabi Meamari

¹ Department of Animal Science, University of Khuzestan Agricultural Sciences and Natural Resources, Khuzestan, Iran.

² Department of Animal Sciences, Ramin Agriculture and Natural Resources University, Khuzestan, Khuzestan, Iran.

10.22067/ijasr.v6i3.43297

Abstract

The aim of the current study is to amplification the regularity region encompassed the mutations changing the Myostatin gene express and also to determine sequential analysis. The blood samples taken from 12 Najdi Cattles in Najdi Cattle station in Shushtar city, Khuzestan province. Then the DNA extracted to amplification 730bp and 561bp fragments. Sequencing was performed after the precision in PCR products put-upon Agarose gel 1% and Myostatin gene pro-motor was determined. In Myostatin gene’s pro-motor region, three mutations, Insertion, Deletion and Substitution in 561bp fragment and the two mutations, Deletion and Substitution in 730bp were appeared, results pointed out. Reported mutations on boxes and pro-motor components did not influence upon Myostatin gene.

Keywords

• Myostatin gene
• Mutation
• Doubled-muscle
• Najdi Cattle

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