Analyses of genetic variation in Khorasan indigenous chicken breed by using of microsatellite mark

Document Type : Scientific - Research Articles

Authors

• Mahdi Nikbakhti ¹
• Hamid Reza Mirzaee ¹
• Majid Afsharian Shandiz ²
• Mohammadreza Mohammad Abadi ³
• Davoud Ali Saghi ⁴

¹ Department of Animal Science, Zabol Faculty of Agriculture, Zabol, Iran

² Razi Vaccine Serum Research Institute

³ Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

⁴ Khorasan Agricultural and Natural Resources Research and Training Center

10.22067/ijasr.v1i2.1971

Abstract

This study investigated genetic variation within indigenous chicken of Khorasan using four microsatellite markers (MCW5,
MCW16, MCW18 and MCW34). 90 blood samples were collected from this population and phenol-chloroform extraction
method was used to isolate the DNA. The highest and the lowest allele number and effective alleles are shown in MCW5 (6
and 5.293) and MCW34 (4 and 3.138), respectively. The expected heterozygosis varied from 0.8157 (MCW5) to 0.6854
(MCW34). The amount of genetic variation within this population was found 0.74 this could be due to low inbreeding in this
population. The Hardy-Weinberg equilibrium (HWE) test showed that all loci deviated from HWE (p

Keywords

• Genetic variation
• Microsatellite marker
• Indigenous chicken

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References

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