Study of polymorphism in Mazandaran and Esfahan native chicken population using microsatellite markers

Document Type : Genetics & breeding

Authors

• Saeid Esmaeilkhanian ¹
• Saeed Ansari Mahyari ²
• Ardeshir Nejati Javaremi
• Hossein Banabazi ³
• Majid Sadeghian
• Maryam Tatari

¹ Isfahan Agricultural and Natural Resources Research Center

² Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran

³ Department of Biotechnology, Animal Science Research Institute of IRAN (ASRI), Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran

10.22067/ijasr.v6i2.24013

Abstract

In order to studying of genetic variation in Mazandaran and Esfahan native chickens, twenty microsatellite markers were evaluated. These microsatellite markers were MCW0014, MCW0081, MCW0183, MCW0067, MCW0104, MCW0123, MCW0330, MCW0165, MCW0069, MCW0020, MCW0222, LEI0094, MCW0295, MCW0034, MCW0216, ADL0268, ADL0112, ADL0278 and lEI0166. Blood samples of 90 and 150 native chickens of Mazandaran and Esfahan were randomly taken respectively. Genomic DNAs were isolated through optimized and modified salting-out procedure. The number of alleles varied from 1 to 6. In Mazandaran population one locus (MCW0216) and in Esfahan population three loci (MCW0216, MCW67 and MCW222) were monomorphic. The other loci were showed appropriate polymorphism. All the loci except MCW222 and MCW165 in Mazandaran population showed deviations from Hardy-Weinberg equilibrium (p

Keywords

• locus
• Polymorphism
• effective allele
• Heterozygosity

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