Effect of Dietary Olive Leaves (Olea europaea L.) Extracts and/or α-tocopheryl Acetate Supplementation on Performance and Intestinal Morphology of Chickens From 7 to 28 Days of Age

Document Type : Poultry Nutrition

Authors

• Mohammad Javad Agah ¹
• Hasan Nasiri Moghadam ¹
• Abolghasem Golian ²
• Ahmad Reza Raaji ³
• Reza Farhoosh ⁴
• Asghar Zarban ⁵

¹ Department of Animal Sciences, Faculty of Agriculture Ferdowsi University of Mashhad

² Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

³ Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad

⁴ Department of Food Science Industry, Faculty of Agriculture, Ferdowsi University of Mashhad

⁵ Birjand University of Medical Sciences

10.22067/ijasr.v7i1.48327

Abstract

Introduction : Antibiotic feed additives have long been used as growth promoters (10). However, consumers and health authorities increasingly dictate that the use of synthetic food additives should be phased out and, where possible, only natural products should be used. Hence, alternatives from natural sources with potential antioxidant activity are investigated (16). Olive leaves are a good source of several antioxidants including oleuropeoside compounds such as oleuropein and verbascoside, and flavonoid compounds such as luteolin, luteolin-7-glucoside, apigenin-7-glucoside, diosmetin, diosmetin-7-glucoside, rutin and catechin, and simple phenolic compounds such as tyrosol, hydroxytyrosol, vanillin, vanillic acid and caffeic acid. Oleuropein, the most prominent compound, may reach concentrations of 60 - 90 mg/g dried leaves (8). Most of these phenolic compounds have been shown to possess substantial antimicrobial and antioxidant activities in vitro. However, in vivo investigations on the antioxidant and antimicrobial activities of olive leaves as a dietary supplement to food producing animals have not been conducted (33). The present study was designed to evaluate the effects of dietary α-tocopheryl acetate, olive leaf powder (OLP) and olive leaf extract (OLE) as feed additives on the growth performance and small intestinal morphology of male broilers.
Materials and Methods : The experimental protocol was approved by the Animal Care Committee of the Ferdowsi University of Mashhad, Iran. The chickens (120 Ross 308 male birds) were placed at one day old in a single colony in a floor pen containing fresh wood shavings to a depth of 10 cm, and all were fed on a starter ration. At 7 d old, 6 replicate cages containing four chicks selected at random were assigned to each of 5 dietary treatments using a completely randomized design, giving a total of 30 experimental cages. The study was completed at 28 d of age. Dietary treatments included a negative control group (NC) fed a corn soybean meal based diet without feed additive supplementation, a positive control (PC) group fed a diet with 250 mg of α-tocopheryl acetate/kg, a test group OLP fed with 10 g of olive leaf powder/kg, and two test groups remaining OLE fed with 120 or 240 mg of olive leaf extract/kg. The birds were provided with one hour of darkness following a period of 23 h light, from the start to the finish of the study. Each cage was provided with a single feed trough and nipple line, with three nipples per cage, to provide ad libitum access to feed and water. On day 7, 14, 21 and 28, body weight and feed intake were measured after a 6-h fast to determine average daily gain, average daily feed intake, and feed conversion. On d 28, one bird per cage randomly selected and killed to determine small intestinal morphology and lymphoid organ weights. Sample sections (2.5 cm in length) were taken from the middle region of the jejunum. The tissues were stained for measurement of villus length, villus width, crypt depth and thickness of muscle layer. The General Linear Models of SAS (17) were used to analyze all the data.
Results and Discussion : Chicks fed with diet containing 240 mg of OLE/kg had the highest average daily gain. ِِDressing percentage and the percentage of breast muscle in the carcass were significantly higher for chicks fed diets supplemented with OLP and OLE compared with those fed NC diet. Similar to our results, Chinese herbal medicine supplementation enhanced BW gain of female broiler chicks at 7 to 21 day of age, when compared with the non-supplemented group. There were no significant differences in daily feed intake between the different treatments from 7 to 21 day of age (17). Also Christaki et al. (12) found that laying Japanese quails diet supplementation with olive leaves (10 g/kg or 20 g/kg) resulted in a tendency (p = 0.054) for higher egg production percentage. The benefits of dietary olive leaves are possibly due to the presence of polyphenols and particularly oleuropein, the main active component in this material (12). The inclusion of OLP and/or OLE in diet significantly decreased the relative lengths of duodenum. Villus height, crypt depth, epithelial thickness and surface area for absorption villi were significantly increased in diets containing OLP and/or OLE compared with NC fed birds. Intestinal morphology can reveal some information on gut health. A shortening of the villus reflects villus atrophy and a decrease in surface area for nutrient absorption. The crypt can be regarded as the villus factory, and a large crypt indicates fast tissue turnover and a high demand for new tissue (21).The gastro protective potential of OLE is probably related to its ability to maintain the cell membrane integrity, by its ant lipid per oxidative activity that protects the gastric mucosa against oxidative damage and by its ability to strengthen the mucosal barrier, the first line of defense against exogenous damaging agents (14).
Conclusion: The use of OLP and/or OLE as feed additives in diet may affect chick performance and gut health. Although, it seems that further experiments are needed to elucidate the mechanisms underlying the enhanced growth of chickens by OLP and/or OLE.

Keywords

• Broiler
• Olive leaf extract
• Performance
• Intestinal morphology

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References

1- Agostini, P. S., D. Sol-Oriol, M. Nofrarı´as, A. C. Barroeta, J. Gasa, and E. G. Manzanilla. 2012. Role of in-feed clove supplementation on growth performance, intestinal microbiology, and morphology in broiler chicken. Livest. Sci. 147:113–118.

2- Al-Azzawie, H., and M.S. S. Alhamdani. 2006. Hypoglycemic and antioxidant effect of oleuropein in alloxan-diabetic rabbits. Life. Sci. 78:1371–1377.

3- Alcicek, A., M. Bozkurt, and M. Cabuk. 2004. The effect of a mixture of herbal essential oils, an organic acid or a probiotic on broiler performance. Sout. Afr. J. Anim. Sci. 34 (4):217-222.

4- Amad, A. A., K. Manner, K. R. Wendler, K. Neumann, and J. Zentek. 2011. Effects of a phytogenic feed additive on growth performance and ileal nutrient digestibility in broiler chickens. Poult. Sci. 90: 2811–2816.

5- Amaral, J. S., R. M. Seabra, P. B. Andrade, P. Valentao, J. A. Pereira, and F. Ferreres. 2004. Phenolic profile in the quality control of walnut Juglans regia L. leaves. Food Chem. 88: 373–379.

6- AOAC. 2005. Official Methods of Analysis. 18th ed. Association of Analytical Chemists, AOAC International, Arlington VA.

7- Banerjee, A., N. Dasgupta, and B. De. 2005. In vitro study of antioxidant activity of Syzygium cumini fruit. Food Chem. 90:727-733.

8- Benavente-Garcia, O., J. Castillo, J. Lorente, A. Ortuño, and J. A. Del Rio. 2000. Antioxidant activity of phenolics extracted from Olea europaea L. leaves. Food Chemistry. 68:457-462.

9- Botsoglou, E., A. Govaris, E. Christaki, and N. Botsoglou. 2010. Effect of dietary olive leaves and/or α-tocopheryl acetate supplementation on microbial growth and lipid oxidation of turkey breast fillets during refrigerated storage. Food Chemistry.121:17-22.

10- Botsoglou, N. A., E. Christaki, P. Florou-Paneri, I. Gieannenas, G. Papageorgiou, and A. B. Spais. 2004. The effect of a mixture of herbal essential oils or α–tocopheryl acetate on performance parameters and oxidation of body lipid in broilers. S. Afr. J. Anim. Sci. 34, 52-61.

11- Burkholder, K. M., K. L. Thompson, M. E. Einstein, T. J. Applegate, and J. A. Patterson. 2008. Influence of stressors on normal intestinal microbiota, intestinal morphology, and susceptibility to salmonella enteritidis colonization in broilers. Poult. Sci.87:1734–1741.

12- Christaki, E. V., E. M. Bonos, and P. C. Florou-Paneri. 2011. Comparative evaluation of dietary oregano, anise and olive leaves in laying Japanese quails. Braz. J. Poultry. Sci.13: 97-101.

13- Cross, D. E., R. M. McDevitt, K. Hillman, and T. Acamovic. 2007. The effect of herbs and their associated essential oils on performance, dietary digestibility and gut microflora in chickens from 7 to 28 days of age. Brit. Poult. Sci. 48:496-506.

14- Dekanski, D., S. Ristić, and D. M. Mitrović. 2009. Antioxidant effect of dry olive (Olea europaea L.) leaf extracton ethanol-induced gastric lesions in rats. Mediterr. J. Nutr. Metab.2:205–211.

15- Garcia, V., P. Catala-Gregori, F. Hernandez, M. D. Megias, and J. Madrid. 2007. Effect of Formic Acid and Plant Extracts on Growth, Nutrient Digestibility, Intestine Mucosa Morphology, and Meat Yield of Broilers. J. Appl. Poult. Res. 16:555–562.

16- Govaris, A., E. Botsoglou, A. Moulas and N. Botsoglou. 2010. Effect of dietary olive leaves and rosemary on microbial growth and lipid oxidation of turkey breast during refrigerated storage. Sout. Afr. J. Anim. Sci.40:145-155.

17- Guo, F. C., R. P. Kwakkel, J. Soede, B. A. Williams, and M.W. A. Verstegen. 2004. Effect of a Chinese herb medicine formulation, as an alternative for antibiotics, on performance of broilers. Brit. Poult. Sci.45: 793–797.

18- Haddadin, M.S. Y. 2010. Effect of olive Leaf extracts on the growth and metabolism of two probiotic bacteria of intestinal origin. Pak. J. Nut.9: 787-793.

19- Hassanpour, H., A. K. Zamani Moghaddam, A. Yazdani, and M. Cheraghchi Bashi. 2010. Evaluation of intestinal morphology and nitric oxide metabolites in broiler chickens supplemented by green tea. Comp. Clin. Pathol.19:43–47.

20- Jamroz, D., T. Wertelecki, M. Houszka, and C. Kamel. 2006. Influence of diet type on the inclusion of plant origin active substances on morphological and histochemical characteristics of the stomach and jejunum walls in chicken. J. Anim. Physiol. Anim. Nutr. 90:255–268.

21- Maa, Y. L., and T. Guob. 2008. Intestinal morphology, brush border and digesta enzyme activities of broilers fed on a diet containing Cu2+-loaded montmorillonite. Brit. Poult. Sci. 49:65-73.

22- Molan, A. L., J. Flanagan, W. Wei, and P. J. Moughan. 2009. Selenium-containing green tea has higher antioxidant and prebiotic activites than regular green tea. Food Chem.114:820-835.

23- Molina-Alcaide, E., and D. R. Yanez-Ruiz. 2008. Potential use of olive by-products in ruminant feeding: A review. Anim. Feed Sci. Technol.147:247-464.

24- Mottaghitalab, M., and M. Jamshid zehi. 2007. Effects of olive leaf powder extract as substitution for Antibiotics on broiler performances. Pages 831-834 in 2th Congress on Animal and Aquatic Sciences. 16-17 May 2007. (In Persian with English abstract).

25- Mountzouris, K. C., P. Tsirtsikos, E. Kalamara, S. Nitsch, G. Schatzmayr, and K. Fegeros. 2007. Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium Enterococcus, and Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities. Poult. Sci. 86:309–317.

26- Nobakht, A., A. R. Safamehr, J. Norani, and M. Moghaddam. 2011. The effects of using different levels of pennyroyal (Mentha pulegium) medicinal plant on performance in broilers and laying hens. Animal Sciences Journal (Pajouhesh & Sazandegi). 92: 41-51. (In Persian with English abstract).

27- Rafiee, Z., S. M. Jafari, M. Khomeiri, and M. Alami. 2011. Effect of variety and method of extraction on antioxidant and antimicrobial activity of alive aeaf extracts. Iranian Food Science and Technology Research Journal. 4: 297-307. (In Persian with English abstract).

28- Sakamoto, K., H. Hirose, A. Onizuka, M. Hayashi, N. Futamura, Y. Kawamura, and T. Ezaki, 2000. Quantitative study of changes in intestinal morphology and mucus gel on total parenteral nutrition in rats. Journal of Surgical Research. 94:99-106.

29- Samarth, R. M., M. Panwar, M. Kumar, A. Soni, M. Kumar, and A. Kumar. 2007. Evaluation of antioxidant and radical scavenging activities of certain radio protective plant extracts. Food Chem. 106:868-873.

30- Sarica, S., A. Ciftci, E. Demir, K. Kilinc, and Y. Yildirim. 2005. Use of an antibiotic growth promoter and two herbal natural feed additives with and without exogenous enzymes in wheat based broiler diets. Sout. Afr. J. Ani. Sci. 35: 61-72.

31- SAS Institute. 2008. SAS Stat User's Guide. Version 9.2 ed. SAS Inst. Inc., Cary, NC.

32- Varmaghany, S., S. Rahimi, M. A. Karimi Torshizi, H. Lotfollahian, and M. Hassanzadeh. 2012. The effect of different levels of olive leaf powder on growth performance in broilers reared at normal and cold temperature. Pages 1079-1082 in 5th Iranian Congress on Animal Science. 30-31August 2012. (In Persian with English abstract).

33- Windisch, W., K. Schedle, C. Plitzner, and A. Kroismayr. 2008. Use of phytogenic products as feed additives for swine and poultry. J. Anim. Sci. 86:E140–E148.

34- Yamauchi, K., K. Yamamoto, and Y. Ishiki. 1995. Morphological alterations of the intestinal villi and absorptive epithelial cells in each intestinal part in fasted chickens. Jpn. Poult. Sci. 32:241-251.