Study of a Recombinant Lactococcuslactis Performance to Degrade of phytate phosphorus in Broiler Chick Diets

Document Type : Poultry Nutrition

Authors

• Hasan Kermanshahi
• Reza Majidzadeh Heravy

Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

10.22067/ijasr.v8i1.41713

Abstract

Introduction Phytic acid is a main compound in all plant seeds and contains 60 to 70% of total phosphorus in plant. Monogastric animals cannot use phytatephosphorus because of low phytase activity in their digestive tract. Thus in addition to unabsorbed mineral phosphorus, phytate phosphorus in fecal ofmonogastric animals may affect water and environment pollution. It is suggested to use of phytase enzyme for resolving of this problem. Phytase activity was showed in some bacteria like pseudomonas, bacillus subtilis and amyloliquefaciens. Few strain of lactic acid bacteria order showed phytase activity or were different for this activity. On the other hand these bacteria order are important as probiotic strains. There are numerous studies to use probiotic bacteria with specific enzyme activity for increasing nutrient availability to animals. This experiment was aimed to study of a recombinant Lactococcuslactis performance to degrade of phytate phosphorus in broiler chicken diets.
Materials and methods Lactococcuslactis was supplemented to a phosphorus deficient diet (50% of available phosphorus recommended by NRC) lonely or with two other lactobacillus bacteria (lactobacillus crispatusand lactobacillus salivarus) in rate of 108 CFU/g diet. Diets were also formulated with recommended available phosphorus by NRC with or without probiotic supplementation. Two hundred eighty eight one day old male Ross broiler chicks were subjected to 6 experimental treatment in 4 replicates and 12 chicks in each replicate. Growth performance, bone characteristics, intestinalmicroflora, and blood metabolites were measured. Apparent phytate digestibility was estimated by chromic oxide marker method. The chromic oxide was added in rate of 0.3% ofthediets. Phytate phosphorus was measured by the method of wheeler et al (1971). On day37, level of calcium, phosphorus and cholesterol of serum were measurement by bleeding from wing vein. Left thigh bone was isolated on 42 days and its dimensions and strength were measurement by Caliper and Instron instruments respectively.
Results and Discussion Body weights of deficiency phosphorus treatments showed significant decrease even in presence of recombinant probiotic (P

Keywords

• Broiler chicks
• Lactococcuslactis
• phytase
• Probiotic
• Recombinant

20.1001.1.20083106.1395.8.1.13.2

---

References

1- Afkhami, M., H. Kermanshahi., and A. Golian. 2014. Nutrative value of three soybeam meals through in vitro techniques and in vivo evaluation in broiler chickens. Iranian journal of animal science, 6(1):8-16.(In Persian)

2- Askelson, T. E., A. Campasino., J. T. Lee., and T. Duong. 2014. Evaluation of phytate-degrading Lactobacillus culture administration to broiler chickens. Applied and Environmental Microbiology, 80(3):943-950.

3- Boling-Frankenbach, S. D., J. L. Snow., C. M. Parsons., and D. H. Baker. 2001. The effect of citric acid on the calcium and phosphorus requirements of chicks fed corn-soybean meal diets. Poultry Science, 80(6):783-788.

4- Boling, S. D., D. M. Webel., I. Mavromichalis., C. M. Parsons., and D. H. Baker. 2000. The effects of citric acid on phytate-phosphorus utilization in young chicks and pigs. Journal of Animal Science, 78(3):682-689.

5- Carew, L. B., J., T. A. Gestone., F. A. Alster., and C. G. Scanes. 1985. Effect of phosphorus deficiency on thyroid function and growth hormone in the white Leghorn male. Poultry Science, 64(10):2010-2012.

6- Dansky, L. M., and F. W. Hill. 1952. Application of the chromic oxide indicator method to balance studies with growing chickens. Journal of Nutrition, 47(3):449-459.

7- Graf, E. 1983. Formation of [3H, 32P]phytic acid in germinating wheat. Anal Biochem 131(2):351-355.

8- Hammes, W. P. and R. F. Vogel. 1995. The genus Lactobacillus. Pages 19-54 in The lactic acid bacteria. Vol. 2. Wood B.J.B. and Holzapfel W.H., ed. Blackie Academic and Professional, London.

9- Lan, G. Q., N. Abdullah., S. Jalaludin., and Y. W. Ho. 2002. Efficacy of supplementation of a phytase-producing bacterial culture on the performance and nutrient use of broiler chickens fed corn-soybean meal diets. Poultry Science, 81(10):1522-1532.

10- Lei, X. G. and C.H. Stahl. 2001. Biotechnological development of effective phytases for mineral nutrition and environmental protection. Applied Microbiology and Biotechnology, 57(4):474-481.

11- Majidzadeh Heravi, R., H. Kermanshahi., M. Sankian., M. R. Nassiri., A. H. Moussavi., L. R. Nasiraii., and A. R. Varasteh. 2011. Screening of lactobacilli bacteria isolated from gastrointestinal tract of broiler chickens for their use as probiotic. African Journal of Microbiology Research, 5:1858-1868.

12- Maynard, L. A. and J. K. Loosli. 1969. Animal Nutrition, 6th ed. McGrow-Hill, New York, USA

13- Mohammadbagheri, N. and R. Najafi. 2014. Study the effect of citric acid and phytase supplementation on performance, blood lipid, immune system and some carcass chracteristics of broiler chickens Iranian journal of animal science, 6(2):131-139. (In Persian)

14- National Research Councile. 1994. Nutrient requirement of poultry. 9 ed. NationalAcademy, Washington, DC.

15- Nourmohammadi, R., S. M. Hosseini., and H. Farhangfar. 2010. Effect of dietatry acidification on some blood parameters and weekly performance of broiler chickens. journal of animal veterinary advances, 9(24):3092-3097.

16- SAS Institute. 2004.SAS/STAT User’s Guide: Statistics. Version 9.2 Edition. SAS Inst. Inc., Cary, NC.

17- Scholz-Ahrens, K. E., P. Ade., B. Marten., P. Weber., W. Timm., Y. Acil., C. C. Gluer., and J. Schrezenmeir. 2007. Prebiotics, probiotics, and synbiotics affect mineral absorption, bone mineral content, and bone structure. Journal of Nutrition, 137(3 Suppl 2): 838S-846S.

18- Sreeramulu, G., D. S. Srinivasa., K. Nand., and R. Joseph. 1996. Lactobacillus amylovorus as a phytase producer in submerged culture. Letters in Applied Microbiology, 23: 385-388.

19- Sweeten, J. 1992. Livestock and poultry waste management: a national overview. Pages 4-15 in National livestock, poultry and aquaculture waste management. Blake JD M.W., ed. Amer Soc Agric Eng, St. Joseph, Minnesota.

20- wheeler E. L., and Ferrel R. E. 1971. A Metjod for phytic acid determination in wheat and wheat fractions. Cereal Chemistry, 48:312-320.

21- Yu, B., J. R. Liu., F. S. Hsiao., and P. W. S. Chiou. 2008. Evaluation of Lactobacillus reuteri Pg4 strain expressing heterologous -glucanase as a probiotic in poultry diets based on barley. Animal Feed Science and Technology, 141: 82–91.

22- Zamudio, M., A. Gonzalez., and J. A. Medina. 2001. Lactobacillus plantarum phytase activity is due to non-specific acid phosphatase. Letters in Applied Microbiology, 32(3): 181-184.