Effect of Administration of Acepromazine Combined with Multi vitamin and Amino Acids Complex on Fertility Outcome, Blood Hematological and Biochemical Alterations and the Oxidative Stress Generated by Laparoscopic AI in Ewes

Document Type : Research Articles

Authors

Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

Abstract

Introduction: Farm animals face different types of abiotic stresses due to many management activities. Stress has adverse consequences on the animal's health and welfare. Laparoscopic artificial insemination (LAI) in sheep is also one of the mini surgery activities that associated with the use of sedatives, catching and fettering the animal, the insertion of instruments into the abdomen, and manipulation of the reproductive tract. All these actions are stressful and may impact stress axis, hematological and biochemical alterations, antioxidant status and fertility outcome. The administration of acepromazine combined with a multivitamin and amino acids complex may be a useful strategy for reducing stress in sheep undergoing laparoscopic AI.
Materials and Methods: In this experiment, 50 non-pregnant Afshar breed ewes, aged 3-4 years, with almost the same body weight and score, were used. The estrous cycle of the ewes was synchronized by flurogestone acetate loaded sponges and eCG hormone. At the time of sponge removal, the ewes were divided into two groups (n = 25). All of the ewes, 54 h after sponge removal, were exposed to stress caused by LAI. In the first group (treatment), with sponge removal, each ewe was received 10 ml of the Multiaminoject intramuscularly. Also, 20 min before LAI, in addition to Multiaminoject, each animal also received 0.0834 mg aspromazine (i.v) per kg of body weight. At the same time, the ewes of the second group were injected with physiological saline and were considered as control. Changes in plasma cortisol concentration and its kinetics were measured from zero (20 min before LAI) to 180 min after through serial blood sampling. Changes of hematological and biochemical parameters of jugular vein blood were evaluated 20 min before and 40 min after LAI. The plasma antioxidant status was measured at the times of sponge removal, LAI and 3 days after LAI. The pregnancy rate was recorded by ultrasound at 45 days.
Results and Discussion: Laparoscopic AI induced stress response in the ewes, by that, 20 minutes after LAI, the concentration of cortisol increased significantly compared to the baseline concentration (P< 0.05). In this research, the injection of the aspromazine along with a multivitamins and amino acids complex could not inhibit the secretion of cortisol during laparoscopic surgery, and it caused the rejection of the hypothesis of this research. The reason for this discrepancy is related to the type of sedative used to reduce or eliminate pain during surgery. It has been reported that plasma cortisol response is reduced by ketoprofen and completely removed by detomidine. As a result, after LAI, the number of white blood cells and the plasma concentration of malodaldehyde increased and the hematocrit, hemoglobin and total antioxidant capacity decreased (P< 0.05), but the concentration of plasma proteins did not change (P> 0.05). Acute stressors cause a transient increase in the number of blood cells in the blood circulation. The cause of this phenomenon is the contraction of the spleen due to the stimulation of catecholamines. In addition, with the increase in cortisol secretion, blood cells are released from the bone marrow into the bloodstream. In a study, the use of supplements containing various vitamins and minerals, one week before and one week after laparoscopic surgery, improved the antioxidant status of patients after surgery and the level of plasma malodaldehyde decreased significantly. The reason for not seeing a significant effect on antioxidant parameters in this research may be the type of supplement containing antioxidants and the time of its use before and after surgery. Injection of aspromazine combined with Multiaminoject increased plasma proteins and decreased the level of aspartate aminotransferase enzyme (P< 0.05). Depending on its intensity and type, stress causes damage and release of tissue and liver enzymes into the blood. In a research, it was shown that the plasma concentration of aspartate aminotransferase and creatine kinase increases due to transportation stress in pigs. The protective effects of vitamin and amino acid supplements in preventing liver damage have been reported in previous studies. The decrease in enzyme concentration after laparoscopy in the treatment receiving aspromazine and multi amino acids may be related to its protective effects. The results of the study showed that there was no significant difference in fertility outcomes between the two groups (P< 0.05). The pregnancy rate for the acepromazine and Multiaminoject-treated group was 50%, while the pregnancy rate for the control group was 45%. This result is probably caused by the lack of interference or partial interference of aspromazine with uterine contractions around ovulation and conception.
Conclusion: The authors concluded that acepromazine combined with a multivitamin and amino acids complex, did not have a significant effect on fertility outcome, blood hematological and biochemical alterations and the oxidative stress generated by laparoscopic AI in ewes, but the severity of tissue damage reduce and plasma globulin concentration increase in response to acepromazine combined with a multivitamin and amino acids complex injection. It is important to note that this is just one study and that more research would be needed to confirm these findings.

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Aghamiri, S. M., Samimi, A. S., Hajian, M., Samimi, A. M., & Oroumieh, A. (2022). Effect of xylazine, detomidine, medetomidine and dexmedetomidine during laparoscopic SCNT embryo transfer on pregnancy rate and some physiological variables in goats. BMC Veterinary Research, 18(1), 98.  https://doi.org/10.1186/s12917-022-03194-8
Ali, B., Al-Qarawi, A., & Mousa, H. (2006). Stress associated with road transportation in desert sheep and goats, and the effect of pretreatment with xylazine or sodium betaine. Research in Veterinary Science, 80(3), 343-348.  https://doi.org/10.1016/j.rvsc.2005.07.012
Ali, B. H., & Al-Qarawi, A. (2002). Evaluation of drugs used in the control of stressful stimuli in domestic animals: A review. Acta Veterinaria Brno, 71(2), 205-216.
Apple, J., Minton, J., Parsons, K., & Unruh, J. (1993). Influence of repeated restraint and isolation stress and electrolyte administration on pituitary-adrenal secretions, electrolytes, and other blood constituents of sheep. Journal of Animal Science, 71(1), 71-77.  https://doi.org/10.2527/1993.71171x
Arfuso, F., Fazio, F., Chikhi, L., Aymond, G., Piccione, G., & Giannetto, C. (2022). Acute stress response of sheep to shearing procedures: Dynamic change of cortisol concentration and protein electrophoretic pattern. Animals, 12(7), 862.  https://doi.org/10.3390/ani12070862
Arfuso, F., Rizzo, M., Giannetto, C., Giudice, E., Piccione, G., Fazio, F., Cirincione, R., Cassata, G., & Cicero, L. (2023). Inflammatory-like status and acute stress response in horses after road transport. Scientific Reports, 13(1), 9858.  https://doi.org/10.1038/s41598-023-37069-1
Avila-Jaime, B., Ramos-Zayas, Y., Franco-Molina, M. A., Alvarado-Avila, R., Zamora-Avila, D. E., Fimbres-Durazo, H., Zárate-Ramos, J. J., & Kawas, J. R. (2021). Effects of transportation stress on complete blood count, blood chemistry, and cytokine gene expression in heifers. Veterinary Sciences, 8(10), 231.
Ballou, M., Sutherland, M., Brooks, T., Hulbert, L., Davis, B., & Cobb, C. (2013). Administration of anesthetic and analgesic prevent the suppression of many leukocyte responses following surgical castration and physical dehorning. Veterinary Immunology and Immunopathology, 151(3-4), 285-293.  https://doi.org/10.1016/j.vetimm.2012.11.018
Brearley, J., Dobson, H., & Jones, R. (1990). Investigations into the effect of two sedatives on the stress response in cattle. Journal of Veterinary Pharmacology and Therapeutics, 13(4), 367-377.  https://doi.org/10.1111/j.1365-2885.1990.tb00791.x
Damián, J., & Ungerfeld, R. (2011). The stress response of frequently electroejaculated rams to electroejaculation: hormonal, physiological, biochemical, haematological and behavioural parameters. Reproduction in Domestic Animals, 46(4), 646-650.  https://doi.org/10.1111/j.1439-0531.2010.01722.x
Danyer, E., Bilal, T., Altiner, A., Aytekin, İ., & Atalay, H. (2021). The effect of vitamin E treatment on selected immune and oxidative parameters in Kivircik ewes suffering from transport stress. Journal of Animal Physiology and Animal Nutrition, 105, 34-41.  https://doi.org/10.1111/jpn.13560
De Leon, J. A. D., & Borges, C. R. (2020). Evaluation of oxidative stress in biological samples using the thiobarbituric acid reactive substances assay. JoVE (Journal of Visualized Experiments), (159), e61122. https://doi.org/10.3791/61122
De Lille, A., Silvers, M., Cadario, M., Tran, T., Cage, C., & LeBlanc, M. (2000). Interactions of xylazine and acepromazine with oxytocin and the effects of these interactions on intrauterine pressure in normal mares and mares with delayed uterine clearance. Journal of Reproduction and Fertility. Supplement, (56), 373-379.
Dujovne, C. A., & Zimmerman, H. J. (1969). Cytotoxicity of phenothiazines on Chang liver cells as measured by enzyme leakage. Proceedings of the Society for Experimental Biology and Medicine, 131(2), 583-587.  https://doi.org/10.3181/00379727-131-33931
Fierro, S., Olivera-Muzante, J., Gil, J., & Viñoles, C. (2011). Effects of prostaglandin administration on ovarian follicular dynamics, conception, prolificacy, and fecundity in sheep. Theriogenology, 76(4), 630-639.  https://doi.org/10.1016/j.theriogenology.2011.03.016
GibbsIII, H. M., & Troedsson, M. H. (1995). Effect of acepromazine, detomidine, and xylazine on myometrial activity in the mare. Biology of Reproduction, 52(monograph_series1), 489-493.  https://doi.org/10.1093/biolreprod/52.monograph_series1.489
Gil, A. G., Illera, J. C., Silván, G., Lorenzo, P. L., & Illera, M. (2002). Changes in hepatic and renal enzyme concentrations and heart and respiratory rates in new zealand white rabbits after anesthetic treatments. Journal of the American Association for Laboratory Animal Science, 41(6), 30-32.
Grandin, T. (2021). How to improve livestock handling and reduce stress. Improving Animal Welfare: A Practical Approach (Ed. 3), 84-112.
Hamam, A., & Abou-Zeina, H. (2007). Effect of vitamin E and selenium supplements on the antioxidant markers and immune status in sheep. Journal of Biological Sciences, 7(6), 870-878. https://doi.org/10.3923/jbs.2007.870.878
Han, B., Gao, D., & Wang, Q. (1995). Study on the mechanism of conirol high temperature transport stress in china experimental miniature pig with supplement compound sodium chlobide power. Acta Vet Zootech Sinica, 26, 261-267.
Hoffman, J. R., Ratamess, N. A., Ross, R., Shanklin, M., Kang, J., & Faigenbaum, A. D. (2008). Effect of a pre-exercise energy supplement on the acute hormonal response to resistance exercise. The Journal of Strength and Conditioning Research, 22(3), 874-882. https://doi.org/10.1519/JSC.0b013e31816d5db6
Hsieh, C., & Rajashekaraiah, V. (2021). Ferric reducing ability of plasma: A potential oxidative stress marker in stored plasma. Acta Haematologica Polonica, 52(1), 61-67. https://doi.org/10.5603/AHP.2021.0009
Jobgen, W., Meininger, C. J., Jobgen, S. C., Li, P., Lee, M. J., Smith, S. B., Spencer, T. E., Fried, S. K., & Wu, G. (2009). Dietary L-arginine supplementation reduces white fat gain and enhances skeletal muscle and brown fat masses in diet-induced obese rats. The Journal of nutrition, 139(2), 230-237.  https://doi.org/10.3945/jn.108.096362
Kiyma, Z., Alexander, B., Van Kirk, E., Murdoch, W., Hallford, D., & Moss, G. (2004). Effects of feed restriction on reproductive and metabolic hormones in ewes. Journal of Animal Science, 82(9), 2548-2557.  https://doi.org/10.2527/2004.8292548x
Laher, I. (2014). Systems biology of free radicals and antioxidants (First Ed.). Springer.
Lone, F. (2022). Artificial insemination in sheep-a breakthrough in offing. Applied Veterinary Research, 1(1), e2022001-e2022001.  https://doi.org/10.31893/avr.2022001
McKnight, J. R., Satterfield, M. C., Jobgen, W. S., Smith, S. B., Spencer, T. E., Meininger, C. J., McNeal, C. J., & Wu, G. (2010). Beneficial effects of L-arginine on reducing obesity: potential mechanisms and important implications for human health. Amino Acids, 39, 349-357.  https://doi.org/10.1007/s00726-010-0598-z
Nayyar, S., & Jindal, R. (2010). Essentiality of antioxidant vitamins for ruminants in relation to stress and reproduction. Iranian Journal of Veterinary Research, 11(1), 1-9.
Önder, N. T., Batı, Y. U., Gökdemir, T., Kılıç, M. C., Şahin, O., Öğün, M., Akyüz, E., Kuru, M., Kırmızıgül, A. H., & Yıldız, S. (2023). Oxidative response of sheep to transcervical applications. Reproduction in Domestic Animals.  https://doi.org/10.1111/rda.14361
Parker, A., Hamlin, G., Coleman, C., & Fitzpatrick, L. (2004). Excess cortisol interferes with a principal mechanism of resistance to dehydration in Bos indicus steers. Journal of Animal Science, 82(4), 1037-1045.  https://doi.org/10.2527/2004.8241037x
Priskas, S., Valergakis, G., Tsakmakidis, I., Vouraki, S., Papanikolopoulou, V., Theodoridis, A., & Arsenos, G. (2022). The role of housing conditions on the success of artificial insemination in intensively reared dairy ewes in greece. Animals, 12(19), 2693.  https://doi.org/10.3390/ani12192693
Raza, S., Tewari, A., Rajak, S., & Sinha, R. A. (2021). Vitamins and non-alcoholic fatty liver disease: A molecular insight. Liver Research, 5(2), 62-71.  https://doi.org/10.1016/j.livres.2021.03.004
Sathe, S. R. (2018). Laparoscopic Artificial insemination technique in small ruminants—A procedure review. Frontiers in Veterinary Science, 5, 266.  https://doi.org/10.3389/fvets.2018.00266
Sciorsci, R., Piccinno, M., & Rizzo, A. (2018). Scopolamine butylbromide decreases the xylazine-mediated contractility in bovine pregnant uteri. Theriogenology, 108, 348-353.  https://doi.org/10.1016/j.theriogenology.2017.12.033
Small, A., Fisher, A. D., Lee, C., & Colditz, I. (2021). Analgesia for sheep in commercial production: Where to next? Animals, 11(4), 1127.  https://doi.org/10.3390/ani11041127
Stafford, K., Chambers, J., Sylvester, S., Kenyon, P., Morris, S., Lizarraga, I., & de Nicolo, G. (2006). Stress caused by laparoscopy in sheep and its alleviation. New Zealand Veterinary Journal, 54(3), 109-113.  https://doi.org/10.1080/00480169.2006.36621
Tsuda, Y., Murakami, R., Yamaguchi, M., & Seki, T. (2020). Acute supplementation with an amino acid mixture suppressed the exercise-induced cortisol response in recreationally active healthy volunteers: A randomized, double-blinded, placebo-controlled crossover study. Journal of the International Society of Sports Nutrition, 17(1), 39.  https://doi.org/10.1186/s12970-020-00369-2
Yardimci, M., Sahin, E., Cetingul, I., Bayram, I., Aslan, R., & Sengor, E. (2013). Stress responses to comparative handling procedures in sheep. Animal, 7(1), 143-150.  https://doi.org/10.1017/S1751731112001449
Yiannakopoulou, E. C., Nikiteas, N., Perrea, D., & Tsigris, C. (2013). Effect of laparoscopic surgery on oxidative stress response: Systematic review. Surgical Laparoscopy Endoscopy and Percutaneous Techniques, 23(2), 101-108. https://doi.org/10.1097/SLE.0b013e3182827b33
Yu, H., Bao, E. D., Zhao, R. Q., & Lv, Q. X. (2007). Effect of transportation stress on heat shock protein 70 concentration and mRNA expression in heart and kidney tissues and serum enzyme activities and hormone concentrations of pigs. American Journal of Veterinary Research, 68(11), 1145-1150.  https://doi.org/10.2460/ajvr.68.11.1145
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  • Receive Date: 10 July 2023
  • Revise Date: 13 November 2023
  • Accept Date: 02 December 2023
  • First Publish Date: 02 December 2023