Effects of Dietary Organic Chromium Supplementation on Laying Performance, Egg Quality, Immune and Blood Biochemical Parameters of Laying Hens in the Late Phase of Production

Document Type : Research Articles

Authors

• Mehrdad Karimi
• Majid Olyayee
• Hossein Janmohammadi
• RUhollah Kianfar
• Fatemeh Khademnasi

Department of Animal Science, Faculty of Agriculture, University of Tabriz. Iran

10.22067/ijasr.2023.80958.1127

Abstract

Introduction: Although minerals include a small part of the total cost of poultry feed, they perform special functions in the body. Given that today's birds are primarily raised under stressful conditions (including high production demands, environmental stress, heat, and exposure to various other stress factors), their nutrient metabolism, including the absorption of minerals, has changed. This has led to decreased feed consumption, reduced mineral absorption, and consequently, increased excretion of minerals. Minerals that serve as cofactors for many enzymes are crucial; thus, to support proper functioning and enhance the immune system's response, higher amounts of these nutrients are required in diets. Chromium (Cr) is a critical micronutrient for humans and animals. It contributes to physiological and nutritional efficiency. Chromium increases glucose glycogenesis, promotes glucose transport, and increases protein synthesis. Chromium is important in protein digestion and reduction of lipid peroxidation and increased growth production. Chromium deficiency is associated with reduced growth rate, and glucose, and protein metabolism. Under environmental, nutritional, and hormonal stress, the benefits of chromium supplementation clearly demonstrated. Considering that with the increase in age and egg size, the egg quality decreases, and also the function of the immune system decreases at the late phase of egg production. Therefore, supplementation of layer hen diets with some nutrients at the end of the production period can improve the egg quality and potentiate the humoral immune response. Thus, the subject of the current study was to investigate the effects of dietary supplementation of chromium-methionine on egg laying performance, egg quality traits at different storage times and temperatures, humoral immune response, and some blood biochemical parameters of laying hens at late–phase of the egg production cycle.
Materials and Methods: A total of 120 Leghorn laying hens (Hy-line W36, 73 weeks- old) were assigned in a completely randomized design with 6 dietary treatments, 5 replicates and 4 laying hens in each replicate for 6 weeks. The experimental treatments were 6 levels of chromium-methionine supplementation (0, 400, 800, 1200, 1600, and 2000 μg/kg of diet) of corn-soybean meal-based diet. The hens were housed in a 60 × 60 × 40 cm cage. All hens were housed in an environmentally controlled house with temperature maintained at approximately 25ºC. The house had controlled ventilation and lighting (16L: 8D). All hens were supplied with feed and water ad libitum. The hen-day egg production, egg weight, egg mass, feed intake, and feed conversion ratio were recorded daily. The feed conversion ratio was expressed in kilograms of feed consumed per kilogram of the egg mass. The effects of organic chromium supplementation of laying hens on egg quality traits were evaluated every 2 weeks at 75, 77, and 79 weeks of age. The egg shape index was calculated by dividing egg length to egg width. At the end of the experiment, to measure certain blood parameters, one bird from each replicate was selected, and blood was drawn from the wing vein. After preparing the serum, the concentrations of triglycerides, cholesterol, total protein, albumin, globulin, uric acid, alanine transaminase, aspartate transaminase, glucose, and malondialdehyde were measured using Pars Azmoon kits according to the manufacturer's instructions.
Results and Discussion: Supplementing the diet with chromium-methionine had a significant effect on laying performance (including feed intake, feed conversion ratio, and egg production) and egg quality traits (such as eggshell weight, yolk color, yolk weight, albumen height, and Haugh unit). The results showed that birds fed 2000 µg/kg chromium-methionine had the lowest feed intake and the best feed conversion ratio and egg production percentage were observed with the addition of 1600 and 2000 g/kg organic chromium. By suppressing stress factors, chromium can increase the ovulation rate and increase the percentage of egg production. Addition of organic chromium except 2000 μg/kg improved albumen height and Haugh unit compared to the control group. The interaction effect between egg storage time and storage temperature on the yolk height and yolk percentage, albumen and yolk pH were significant. Carotenoids are responsible for the color of the yolk and they are a part of the fat-soluble compounds, so any factor that can improve the digestion and absorption of fats will probably lead to an increase in the color of the egg yolk. Haugh unit is a measure to determine the quality of egg white, which determines the quality of the egg. The higher HU means better egg quality (fresher, higher quality eggs have thicker whites). Blood serum cholesterol of chickens fed with 800 µg/kg of chromium-methionine decreased compared to the control group. The lowest serum concentration of malondialdehyde was observed using 1600 and 2000 µg/kg organic chromium. Addition of 1600 µg/kg of chromium-methionine increased total immunoglobulin production against sheep red blood cells. The effects of dietary chromium supplementation on improving immune responses can be attributed to its antioxidant activity. By enhancing antioxidant activity, chromium reduces the production of corticosterone, a potent suppressor of immune system function. This reduction in corticosterone levels prevents the decrease in lymphocyte production and proliferation, ultimately supporting the continued production of antibodies.
Conclusion: In total, dietary supplementation of 1600 μg/kg chromium-methionine can improve egg laying performance including feed conversion ratio, egg production, and egg mass, as well as egg quality traits such as egg shell weight and albumen height of laying hens at the late-phase of laying cycle. Supplementation of layer diets with 1600 μg/kg organic chromium improved the humoral immune response at the late-phase of egg production cycle by increasing the production of total immunoglobulin against sheep red blood cells.
 

Keywords

• Humoral immunity
• Egg quality
• Chromium-methionine
• Blood parameters
• Laying hens

Main Subjects

• Poultry Nutrition

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©2023 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source

References

Habibian, M., Ghazi, S., & Moeini, M. M. (2013). Lack of effect of dietary chromium supplementation on growth performance and serum insulin, glucose, and lipoprotein levels in broilers reared under heat stress condition. Biological Trace Element Research, 153(1), 205-211. https://doi.org/10.1007/s12011-013-9663-2

Hayirli, A. (2005). Chromium nutrition of livestock species. In Nutrition Abstracts and Reviews. Series B. Livestock Feeds and Feeding, 75(5). CAB International.

Hossain, S.M. (1998). Organic chromium in poultry: Metabolic responses, effects on broiler carcass composition, nutrient composition of eggs. In: Lyons, T. P. and K. A. Jacques (Eds.). Biotech in the Feed Industry. Nottingham University Press, Nottingham, UK 203-216.

Jahanian, R., & Mirfendereski, E. (2015). Effect of high stocking density on performance, egg quality, and plasma and yolk antioxidant capacity in laying hens supplemented with organic chromium and vitamin C. Livestock Science, 177, 117-124. https://doi.org /10.1016/ j.livsci. 2015.04.022

Jeejeebhoy, K. N., Chu, R. C., Marliss, E. B., Greenberg, G. R., & Bruce-Robertson, A. (1977). Chromium deficiency, glucose intolerance, and neuropathy reversed by chromium supplementation, in a patient receiving long-term total parenteral nutrition. The American Journal of Clinical Nutrition, 30(4), 531-538. https://doi.org/10.1093/ajcn/30.4.531

Karami, M., Torki, M., & Mohammadi, H. (2018). Effects of dietary supplemental chromium methionine, zinc oxide, and ascorbic acid on performance, egg quality traits, and blood parameters of laying hens subjected to heat stress. Journal of Applied Animal Research, 46(1), 1174-1184. https://doi.org/10.1080/09712119.2018.1481411

Khan, R. U., Naz, S., Dhama, K., Saminathan, M., Tiwari, R., Jeon, G. J., Laudadio, V & Tufarelli, V. (2014). Modes of action and beneficial applications of chromium in poultry nutrition, production and health: A review. International Journal of Pharmacology, 10(7), 357–367. https://doi.org/10.3923/ijp.2014.357.367.

Kim, Y. H., Han, I. K., Shin, I. S., Chae, B. J., & Kang, T. H. (1996). Effect of dietary excessive chromium picolinate on growth performance, nutrient utilizability and serum traits in broiler chicks. Asian-Australasian Journal of Animal Sciences, 9(3), 349-354. https://doi.org/10.5713/ajas.1996.349.

Kuehn, L. A., Price, S. E., Honaker, C. F., & Siegel, P. B. (2006). Antibody response of chickens to sheep red blood cells: crosses among divergently selected lines and relaxed sublines. Poultry Science, 85(8), 1338-41. https://doi.org/10.1093/ps/85.8.1338.

 Lien, T. F., Yang, K. H., Lin, K. J. (2005) Effects of chromium propionate supplementation on growth performance, serum traits and immune response in weaned pigs. Asian-Aust Journal of Animal Science, 18, 403-408. https://doi.org/10.5713/ajas.2005.403.

Lien, T. F., Wu, C. P., & Lu, J. J. (2003). Effects of cod liver oil and chromium picolinatesupplements on the serum traits, egg yolk fatty acids and cholesterol content in laying hens. Asian-Aust. Journal of Animal Science, 16, 1177–1181. https://doi.org/10.5713/ ajas.2003.1177.

Lien, T. F., Chen, S., Shiau, S., Froman, D., & Hu, C. (1996). Y. Chromium picolinate reduces laying hen serum and egg yolk cholesterol. Profess. Animal Science, 12(2), 77–80. https://doi.org/10.15232/S1080-7446(15)32493-1.

Luo, X., Guo, Y. L., Liu, B., Hao, Z. L., Chen, J. L., Gao, F. S., & Yu, S. X. (1999). Effect of dietary chromium on growth, serum biochemical traits and immune responses of broiler chicks during 0–3 weeks of age. Acta Veterinary et Zootechnica Sinica, 30, 481–489.

Ma, W., Gu, Y., Lu, J., Yuan, L., & Zhao, R. (2014). Effects of chromium propionate on egg production, egg quality, plasma biochemical parameters, and egg chromium deposition in late-phase laying hens. Biological trace element research, 157(2), 113–119. https://doi.org/ 10.1007/ s12011-013-9875-5.

Maysa, M. H. (2011). Influence of adding organic chromium in diet on productive traits, serum constituents and immune status of Bandarah laying hens and semen physical properties for cocks in winter season. Egyptian Poultry Science Journal, 31(2), 203-216.

Mowat, D. N. (1997). Organic chromium in animal nutrition. Chromium Books 6 Mayfield Avenue Guelph, ON N1G 2L8 Canada.

Nasiroleslami, M., & Torki, M. (2011). Egg quality characteristics and productive performance of laying hens fed diets supplemented by Echinacea purpurea extract, immunizing and vitamin E. Glob. Veterin, 7(3), 270-275.

Nattapon, S., Jin-Jenn, L., Alex, T., & Tu-Fa Lien, T. (2012). Effect of different level of nanoparticles of Cr picolinate supplementation on performance, egg quality, retention and tissue mineral accumulation in layers. Journal of Agricultural Science, 5(2), 150-159. https://doi.org/10.5539/jas.v5n2p150.

Onderci, M., Sahin, N., Sahin, K., & Kilic, N. (2003). Antioxidant properties of chromium and zinc: In vivo effects on digestibility, lipid peroxidation, antioxidant vitamins, and some minerals under a Low ambient temperature. Biological trace element research, 92(2), 139–150. https://doi.org/10.1385/BTER:92:2:139.

Palvik, A., Pokludova, M., Zapletal, D., & Jelinek, P. (2007). Effect of housing systems on biochemical indicators of blood plasma in laying hens. Acta Veterinary, 76(3), 339-347. https://doi.org/10.2754/avb200776030339.

Pechova, A., & Pavlata, L. (2007). Chromium as an essential nutrient: A review. Veterinary Medicine, 52(1), 1-18. doi: 10.17221/2010-VETMED.

Preuss, H. G., Grojec, P. L., Lieberman, S., & Anderson, R. A. (1997). Effects of different chromium compounds on blood pressure and lipid peroxidation in spontaneously hypertensive rats. Clinical Nephrology, 47(5), 325–330.

Sahin, K., Ozbey, O., Onderci, M., Cikim, G., & Aysondu, M. H. (2002). Chromium supplementation can alleviate negative effects of heat stress on egg production, egg quality and some serum metabolites of laying Japanese quail. The Journal of nutrition, 132(6), 1265–1268. https://doi.org/10.1093/jn/132.6.1265.

Sahin, N., Sahin, K., Onderci, M., Gursu, M. F., Cikim, G., Vijaya, J., Kucuk, O. (2005). Chromium picolinate, rather than biotin, alleviates performance and metabolic parameters in heat stressed quail. British Poultry Science, 46(4), 457-463. https://doi.org/10.1080/00071660500190918

Samanta, S., Haldar, S., Bahadur, V., & Ghosh, T. K. (2008). Chromium picolinate can ameliorate the negative effects of heat stress and enhance performance, carcass and meat traits in broiler chickens by reducing the circulatory cortisol level. Journal of the Science of Food and Agriculture, 88(5), 787–796. https://doi.org/10.1002/jsfa.3146.

SAS. 2004. SAS User’s Guide: Statistics. Version 9.1 Edition. SAS Institute Inc., Cary, NC.

Sirirat, N., Lu, J., Alex, J., Hung, T. Y., & Lien, T. F. (2013). Effect of different levels of nanoparticles chromium picolinate supplementation on performance, egg quality, mineral retention, and tissues minerals accumulation in layer chickens. Journal of Agricultural Science, 5(2), 150-159. https://doi.org/10.5539/jas.v5n2p150.

Subiyatno, A., Mowat, D. N., & Yang, W. Z. (1996). Plasma metabolite response toglucose infusion in dairy cows supplemented with chelated chromium. Journal of Dairy Science, 79, 1436-1445. https://doi.org/10.3168/jds.S0022-0302(96)76502-5.

Torki, M., Zangeneh, S., & Habibian, M. (2014). Performance, egg quality traits, and serum metabolite concentrations of laying hens affected by dietary supplemental chromium picolinate and vitamin C under a heat-stress condition. Biological Trace Element Research, 157(2), 120–129. https://doi.org/10.1007/s12011-013-9872-8.

Uyanik, F., Kaya, S., Kolsuz, A. H., Eren, M., & Sahin, N. (2002). The effect of chromium supplementation on egg production, egg quality and some serum parameters in laying hens. Turkish Journal of Veterinary and Animal Sciences, 26(2), 379-387.

Valera, M.; Gutiérrez, O., & Elías, A. (2019). Organic products based on chromium for poultry production. Main advances in the last years. Cuban Journal of Agricultural Science, 53(3), 219-230.

 Zhang, C. H., Jiang, J. Y., Ren, H. Y., Zhu, F. H., Li, L. W. (2009) Effects of chromium yeast on immune function and carcass quality in heat stressed broilers. Acta Ecologiae Animalis Domastici, 5, 56-61.

Zhang, S., Sun, X., Liao, X., Lu, L., Zhang, L., Ma, Q., & Luo, X. (2018). Dietary supplementation with chromium picolinate influences serum glucose and immune response of brown-egg laying hens. Biological Trace Element Research, 185(2), 448-455. http://doi.org/10.1007/s12011-017-1232-7.