The Effect of Fat-Soluble Vitamins (A, D, E) and Flaxseed Oil on Blood Parameters and Immune System of Suckling Calves

Document Type : Research Articles

Authors

• Mehrdad Movahednasab
• Abdolmansour Tahmasebi
• Seyed Alireza Vakili
• Abbas Ali Naserian

Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

10.22067/ijasr.2022.75288.1067

Abstract

Introduction: One of the most important factors in the profitability of dairy farms is effective management methods in keeping and feeding infant calves. Several factors such as underdeveloped immune system and environmental stress can increase the survival power of the animal. Researchers suggest different factors can to improve the immune system of neonatal calves, including the use of fat-soluble vitamins and unsaturated fatty acids. While these vitamins play a major role in the immune system of animals, the supply of fat-soluble vitamins in the calf's diet is essential for promotion the normal growth of muscles and the skeleton of the body. Common symptoms of a deficiency in fat-soluble vitamins in neonatal calves include retardation of growth, and susceptibility to infectious diseases.
Materials and Methods: This experiment was performed in the farm of Ferdowsi University of Mashhad. Twenty-eight Holstein female calves with an average bodyweight of 37.74 kg (± 4.76) were used from birth to 56 days of age. On the fourth day, the calves were randomly assigned to one of four treatments.  All calves received colostrum for the first 3 d and then whole milk at 8% of bodyweight in the two-equal part in the morning (4.00 A.M) and evening (16 P.M) until weaning. The experimental treatments included: 1) whole milk with starter (Control), 2) control diet supplemented with flax seed oil (0.3 ml per kilogram of body weight), 3) control diet and weekly injection of 7 cc of fat-soluble vitamins (A, D3, E), 4) control diet which supplemented with flaxseed oil (0.3 ml per kilogram of body weight) plus weekly injection of 7 cc of fat-soluble vitamins (A, D3, E). Flax seed oil was mixed into milk (morning feeding) until weaning. During the experiment period, calves had ad libitum access to starter diet and water. Blood sample were harvested from jugular vein for collection of full blood for Complete Blood Count (CBC) test and blood serum metabolites. Serum was stored in -20 centigrade until further analysis. Complete blood count (CBC) test was performed to determine the number of blood cells white blood cells, red blood cells, lymphocytes, monocytes, eosinophils, granulocytes, hemoglobin and hematocrit. Serum blood metabolites, total protein, creatinine, beta-hydroxybutyric acid, albumin, liver enzymes (alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase), antioxidant capacity (malondialdehyde and total antioxidant capacity) were analyzed using Alpha Classic autoanalyzer. Data were analyzed using SAS version 9.4 as a completely randomized design experiment. For all results, significant differences between treatments were declared at P≤ 0.05 and tendencies were declared at 0.05 < P≤ 0.10. Least square means for each treatment are reported in the tables and were separated using Tukey-kramer test.
Results and Discussion: The results of this study showed that the use of flaxseed oil and injection of fat-soluble vitamins during preweaning had no significant effect on the Serum concentrations of total protein, creatinine, beta-hydroxybutyric acid, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, malondialdehyde, total antioxidant capacity and had no significant effect on the starter feed intake, daily weight gain, rectal temperature of calves. Calves received flax seed oil and fat-soluble vitamins injection had the highest Red blood cells, White blood cells, Hematocrit, Lymphocytes, Monocytes, Eosinophils and hemoglobin concentration. Claves received flaxseed oil and fat-soluble vitamins injection have the highest serum cholesterol concentration (P<0.00).
Conclusion: The results of this study indicated that inclusion of flaxseed oil and injection of fat-soluble vitamins (A, D, E) had no significant effect on blood serum parameters such as total protein, creatinine, albumin, beta-hydroxybutyric acid, malondialdehyde and total antioxidant capacity, but partially improved the immune system (white blood cells, monocytes, eosinophils) and reduces liver enzymes such as aspartate aminotransferase and alanine aminotransferase, which indicates an increase in animal health.

Keywords

• Blood parameters
• Fat-soluble vitamins
• Flaxseed oil
• Holstein calves
• Immune system  

Main Subjects

• Animal Nutrition

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References

1. Abu El-Hamd, M. A., Metwally, A. S. M., Hegazy, M. M., Ghallab, Z. R., & Elateeqy, O.A. (2019). Effect of supplementation of omega-3 fatty acids on blood parameters and semen quality of Friesian bulls. Slovenian Veterinary Research, 56. https://doi.org/10.26873/SVR-817-2019
2. Agustinho, B. C., Zeoula, L. M., Santos, N. W., Machado, E., Yoshimura, E. H., Ribas, J. C., Bragatto, J. M., Stemposki, M. R., Santos, V. J. D., & Faciola, A.P. (2020). Effects of flaxseed oil and vitamin e supplementation on digestibility and milk fatty composition and antioxidant capacity in water buffaloes. Animals, 10(8), 1294. https://doi.org/10.3390/ani10081294
3. Ahangarani, M. A., Dehghani, M. R., & Naserian, A. A. (2021). The effect of adding sources of fat to milk on performance, growth, health, blood and ruminal parameters of Holstein female dairy calves in the first month after birth. Animal Production, 23(2),179-189. (In Pershan). https://doi.org/10.22059/jap.2020.293595.623477
4. Asadi, M., Ghoorchi, T., Toghdory, A., Rajabi Aliabadi, R., Iri Tomaj, R., & Sahneh, M. (2021). Comparison of selenium and vitamin E recommended NRC and ARC by diet and injection methods on performance, digestibility, some blood metabolites and skeletal growth indices of suckling Holstein calves. Journal of Animal Science Research, 31(2),57-69. (In Persian). https://doi.org/22034/as.2021.36647.1526
5. Bakowska-Barczak, A., Larminat, M. A., & Kolodziejczyk, P. P. (2020). The application of flax and hempseed in food, nutraceutical and personal care products. In: R. M. Kozlowski and M. M. Talzarczyk, eds. Handbook of natural fibres. Woodhead Publishing, London, UK, 557–590.
6. Bechlin, T. R., Granella, S. J., Christ, D., Coelho, S. R. M., & Viecelli, C. A. (2019). Evaluation of grain and oil quality of packaged and ozonized flaxseed. Journal of Stored Products Research, 83, 311-316. https://doi.org/1016/j.jspr.2019.07.014
7. Beheshti Moghadam, M. H., Rezaei, M., Behgar, M., & Kermanshahi, H. (2019). Effects of gamma and electron radiation on chemical composition and some phyto-chemical properties of whole flaxseed. Journal of Radioanalytical and Nuclear Chemistry, 321, 1019-1025. https://doi.org/10.1007/s10967-019-06679-3
8. Blakely, L. P., Poindexter, M. B., Stuart, R. L., & Nelson, C. D. (2019). Supplementing pasteurized waste-milk with vitamins A, D, and E improves vitamin status of dairy calves. The Bovine Practitioner, 134-141. https://doi.org/10.21423/bovine-vol53no2p134-141
9. Conneely, M., Berry, D. P., Sayers, R., Murphy, J. P., Lorenz, I., Doherty, M. L., & Kennedy, E. (2013). Factors associated with the concentration of immunoglobulin G in the colostrum of dairy cows. Animal, 7(11), 1824-1832. https://doi.org/10.1017/S1751731113001444
10. Delir, S., Mohammmad zadeh, H., Taghizadeh, A., & paya, H., (2020). Effects of application of vitamin-mineral supplement in milk on performances of Holstein suckling calves. Journal of Animal Science Research, 30(3): 105-117. (In Pershan). https://doi.org/22034/as.2021.38432.1557
11. Doppenberg, J., & Palmquist, D. L. (1991). Effect of dietary fat level on feed intake, growth, plasma metabolites and hormones of calves fed dry or liquid diets. Livestock Production Science, 29(2-3), 151-166. https://doi.org/10.1016/0301-6226(91)90063-V
12. Dunford, N. T. (2015). Hemp and flaxseed oil: properties and applications for use in food. In Specialty oils and fats in food and nutrition (pp. 39-63). Woodhead Publishing. https://doi.org/10.1016/B978-1-78242-376-8.00002-8
13. Elgaml, N. B., El-Hamd, A., El-Diahy, Y. M., & Elshora, M. A. (2020). Effect of Flaxseed Oil on Feeding Dairy Friesian Cows on the Fatty Acid and Chemical Composition of Milk and Physiochemical Properties of Yoghurt. Journal of Food and Dairy Sciences, 11(11), 293-298. https://doi.org/21608/jfds.2020.130425
14. El-Hamd, A., El-Diahy, Y. M., El-Maghraby, M. M., & Elshora, M. A. (2015). Effect of flaxseed oil on digestibility, blood parameters, immuno-response and productive performance of suckling Friesian calves. Journal of Animal and Poultry Production, 6(12), 755-765. https://doi.org/21608/jappmu.2015.52963
15. Garcia, M., Shin, J. H., Schlaefli, A., Greco, L. F., Maunsell, F. P., Santos, J. E. P., ... & Thatcher, W. W. (2015). Increasing intake of essential fatty acids from milk replacer benefits performance, immune responses, and health of preweaned Holstein calves. Journal of Dairy Science, 98(1), 458-477. https://doi.org/3168/jds.2014-8384
16. Geraili, M., Torbati Nejad, N.M., Naserian, A.A. & Toghdory, A., (2018). The effect of flaxseed oil on the immune response of calves in heat stress conditions. The 1^st National Conference on Ruminant and Poultry Metabolic Disorders, University of Zanjan, Iran. (In Pershan).
17. Ghorbani, H., Kazemi-Bonchenari, M., HosseinYazdi, M., & Mahjoubi, E. (2020). Effects of various fat delivery methods in starter diet on growth performance, nutrients digestibility and blood metabolites of Holstein dairy calves. Animal Feed Science and Technology, 262, 114429. https://doi.org/10.1016/j.anifeedsci.2020.114429
18. Giarola, T. M. o., Pereira, C. G., Prado, M. E. T., de Abreu, L. R., & de Resende, J. V. (2019). Effects of golden flaxseed flour on ice recrystallization in Uvaia (Eugenia pyriformis Cambess.) diet sherbet. Food and Bioprocess Technology, 12, 2120-2135. https://doi.org/10.1007/s11947-019-02377-w
19. Goyal, A., Patel, A., Sihag, M. K., Shah, N., & Tanwar, B. (2018). Therapeutic potential of flaxseed. In Therapeutic, Probiotic, and Unconventional Foods (pp. 255-274). Academic Press. 1016/B978-0-12-814625-5.00013-3
20. Hill, T. M., Bateman II, H. G., Aldrich, J. M., & Schlotterbeck, R. L. (2009). Effects of changing the essential and functional fatty acid intake of dairy calves. Journal of Dairy Science, 92(2), 670-676. https://doi.org/10.3168/jds.2008-1368
21. Hill, T. M., VandeHaar, M. J., Sordillo, L. M., Catherman, D. R., Bateman Ii, H. G., & Schlotterbeck, R. L. (2011). Fatty acid intake alters growth and immunity in milk-fed calves. Journal of Dairy Science, 94(8), 3936-3948. https://doi.org/10.3168/jds.2010-3935
22. Huuskonen, A., Khalili, H., Kiljala, J., Joki-Tokola, E., & Nousiainen, J. (2005). Effects of vegetable fats versus lard in milk replacers on feed intake, digestibility, and growth in Finnish Ayrshire bull calves. Journal of Dairy science, 88(10), 3575-3581. https://doi.org/10.3168/jds.S0022-0302(05)73042-3
23. Ivan, M., Petit, H. V., Chiquette, J., & Wright, A. D. (2013). Rumen fermentation and microbial population in lactating dairy cows receiving diets containing oilseeds rich in C-18 fatty acids. British Journal of Nutrition, 109(7), 1211-1218. https://doi.org/10.1017/S0007114512003030
24. Kadkhoday, A., Riasi, A., Alikhani, M., Dehghan-Banadaky, M., & Kowsar, R. (2017). Effects of fat sources and dietary C18: 2 to C18: 3 fatty acids ratio on growth performance, ruminal fermentation and some blood components of Holstein calves. Livestock Science, 204, 71-77. https://doi.org/10.1016/j.livsci.2017.08.012
25. Karimi, A., Alijoo, Y. A., Kazemi-Bonchenari, M., Mirzaei, M., & Sadri, H. (2021). Effects of supplemental fat sources and forage feeding levels on growth performance, nutrient digestibility, ruminal fermentation, and nitrogen utilization in dairy calves. Animal, 15(4), 100179. https://doi.org/10.1016/j.animal.2021.100179
26. Lan, Y., Ohm, J. B., Chen, B., & Rao, J. (2020). Physicochemical properties and aroma profiles of flaxseed proteins extracted from whole flaxseed and flaxseed meal. Food Hydrocolloids, 104, 105731. https://doi.org/10.1016/j.foodhyd.2020.105731
27. Lashkari, S., Jensen, S. K., Hansen, C. B., Krogh, K., Theilgaard, P., Raun, B. M., & Vestergaard, M. (2021). Feeding concentrate pellets enriched by natural vitamin E keeps the plasma vitamin E above the critical level in calves post-weaning. Livestock Science, 253, 104672. https://doi.org/10.1016/j.livsci.2021.104672
28. McGee, M., & Earley, B. (2019). passive immunity in beef-suckler calves. Animal, 13(4), 810-825. https://doi.org/10.1017/S1751731118003026
29. McGee, M., Drennan, M. J., & Caffrey, P. J. (2005). Effect of suckler cow genotype on cow serum immunoglobulin (Ig) levels, colostrum yield, composition and Ig concentration and subsequent immune status of their progeny. Irish journal of agricultural and food research, 173-183.
30. Mohri, M., Seifi, H. A., & Khodadadi, J. (2005). Effects of preweaning parenteral supplementation of vitamin E and selenium on hematology, serum proteins, and weight gain in dairy calves. Comparative Clinical Pathology, 14, 149-154. https://doi.org/10.1007/s00580-005-0581-3
31. Moradian, M., Rahchmani, R., Banihasan, E., Gharebash, A.M. & Zeighamy, A., (2016). The effect of injection of vitamins A and E on passive transfer of immunoglobulin G and some blood parameters in calf. Journal of Ruminant Research, 4(2). (In Pershan). https://doi.org/22069/EJRR.2020.18054.1750
32. Nazir, G., Ghuman, S. P. S., Singh, J., Honparkhe, M., Ahuja, C. S., Dhaliwal, G. S., ... & Agarwal, S. K. (2013). Improvement of conception rate in postpartum flaxseed supplemented buffalo with Ovsynch+ CIDR protocol. Animal reproduction science, 137(1-2), 15-22. https://doi.org/1016/j.anireprosci.2012.11.012
33. Newkirk, R. (2015). Flax feed industry guide. Flax Canada, 1-24.
34. Omur, A., Kirbas, A., Aksu, E., Kandemir, F., Dorman, E., Kaynar, O., & Ucar, O. (2016). Effects of antioxidant vitamins (A, D, E) and trace elements (Cu, Mn, Se, Zn) on some metabolic and reproductive profiles in dairy cows during transition period. Polish Journal of Veterinary Sciences, 19(4). https://doi.org/1515/pjvs-2016-0088
35. Opgenorth, J., Sordillo, L. M., Gandy, J. C., & VandeHaar, M. J. (2020). Colostrum supplementation with n-3 fatty acids does not alter calf outcome on a healthy commercial farm. Journal of dairy science, 103(12), 11689-11696. https://doi.org/3168/jds.2019-18046
36. Ramezani, M., Seifdavati, J., Seifzadeh, S., Abdibenemar, H., & Razmazar, V. (2018). The effects of conjugated linoleic acid and vitamin C on growth performance, some blood metabolites and blood cell counts of Holstein suckling calves. Journal of Ruminant Research, 6(2), 101-116. https://doi.org/22069/EJRR.2018.14986.1634
37. Ravisankar, P., Reddy, A. A., Nagalakshmi, B., Koushik, O. S., Kumar, B. V., & Anvith, P. S. (2015). The comprehensive review on fat soluble vitamins. IOSR Journal of Pharmacy, 5(11), 12-28. https://doi.org/13189/ijbb.2018.060103
38. Reddy, P. G., Morrill, J. L., Minocha, H. C., & Stevenson, J. S. (1987). Vitamin E is immunostimulatory in calves. Journal of Dairy Science, 70(5), 993-999. https://doi.org/3168/jds.S0022-0302(87)80104-2
39. Rho, S. J., & Kim, Y. R. (2022). Improving solubility and stability of fat-soluble vitamins (A, D, E, and K) using large-ring cycloamylose. Lebensmittel-Wissenschaft & Technologie, 153, 112502. https://doi.org/1016/j.lwt.2021.112502
40. Sobiech, P. R. Z. E. M. Y. S. Ł. A. W., Żarczyńska, K. A. T. A. R. Z. Y. N. A., Rękawek, W. O. J. C. I. E. C. H., Snarska, A. N. N. A., Eleusizowa, A. N. A. R. A., Kowalczyk, E., & Illek, J. O. S. E. F. (2015). Effect of parenteral supplementation of selenium and vitamin E on selected blood biochemical parameters in HF cows during the transition period. Medycyna weterynaryjna, 71, 683-9. https://doi.org/1016/j.tvjl.2007.06.006
41. Tang, Z. X., Ying, R. F., Lv, B. F., Yang, L. H., Xu, Z., Yan, L. Q., ... & Wei, Y. S. (2021). Flaxseed oil: Extraction, Health benefits and products. Quality Assurance and Safety of Crops & Foods, 13(1), 1-19. https://doi.org/10.15586/qas.v13i1.783
42. Terler, G., Poier, G., Klevenhusen, F., & Zebeli, Q. (2022). Replacing concentrates with a high-quality hay in the starter feed in dairy calves: I. Effects on nutrient intake, growth performance, and blood metabolic profile. Journal of Dairy Science, 105(3), 2326-2342. https://doi.org/3168/jds.2021-21078
43. Thiessen, D. L. (2004). Optimization of feed peas, canola and flaxseed for aqua feeds: The Canadian prairie perspective. Avances en Nutrición Acuicola.
44. Weiss, W. P. (2017). A 100-Year Review: From ascorbic acid to zinc—Mineral and vitamin nutrition of dairy cows. Journal of dairy science, 100(12), 10045-10060. https://doi.org/3168/jds.2017-12935
45. Xu, H. J., Wang, L. H., Zhang, Q. Y., Jiang, X., Zhang, C. R., & Zhang, Y. G. (2021). Effects of 25-hydroxyvitamin D3 on growth performance, fecal scores, vitamin D3 metabolites, antioxidant status, and inflammatory and stress-related parameters in weaning calves. Animal Feed Science and Technology, 281, 114946. https://doi.org/1016/j.anifeedsci.2021.114946
46. Zhang, X., Yin, P., Yang, L., Hang, F., Sun, L., & Liu, Y. (2017). Oil contents in flaxseeds from different origins and fatty acid compositions of flaxseed oils. China Oils and Fats, 42(11), 142-146.