The effect of kefir and peppermint extract (Mentha pipperita) extract in drinking water on performance, lipid profiles, thyroid hormones and testosterone hormone of Japanese quail

Document Type : Research Articles

Authors

• Khalil Mirzadeh ¹
• Amin Kazemizadeh ²
• Zarbakht Ansari Pirsaraei ³

¹ Faculty of Animal Science, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.

² Department of Animal Science. Khuzestan university of Agriculure and Natural Resources, Mollasani, Iran.

³ Animal Science Department, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

10.22067/ijasr.2021.67841.1001

Abstract

Introduction: Application of antibiotics causes two major health problems, including antibiotic residues in body tissues and animal products and the resistance of pathogens to antibiotics. On the other hand, antibiotics improve growth, feed efficiency and control infectious diseases. Following the ban on the use of antibiotics as growth stimulants in the poultry industry, the EU has been paying more attention to non-antibiotic growth-promoting compounds since 2006, including organic acids, probiotics and herbs. Previous studies have shown that in many oils of dark plants Mentha pipperita, thymol, carvacrol, menthol and in some cases paracetamol are the most important components of growth improvement, their antimicrobial and antioxidant properties. kefir is also a natural probiotic. The fermenting agent of kefir milk is kefir seeds, which contain casein and the species Lactobacillus, Saccharomyces, Streptococcus and others. Diets containing Mentha pipperita, by improving the microflora of the gastrointestinal tract and reducing the population of harmful microbes, can create better conditions for the use of final feed products and thus improve the growth and better performance of chickens. In studies, the positive effects of kefir on the performance and biochemical parameters of broiler blood have been reported.
Materials and Methods: This study was conducted in the animal husbandry research station of Khuzestan University of Agriculture and Natural Resources. In this study, four hundred one-day-old Japanese quails were used in a completely randomized design with four treatments, four replications and 25 observations per replication for 35 days. The experiment was performed. Treatments are: Treatment 1) Basic diet + drinking water (control group), Treatment 2) Basic diet + drinking water + 0.5% Mentha pipperita extract, Treatment 3) Basic diet + drinking water + kefir milk 2%, Treatment 4) Basic diet + drinking water + Mentha pipperita extract 0.5% + kefir milk are 2%. The weight of quails was measured at the end of each week and the feed consumed was determined. The average of daily weight gain, feed intake and feed conversion ratio were calculated. At the end of the fifth week, from each replicate, two quails with a weight close to the average were selected and slaughtered.  Breast and thigh meat samples were used to measure pH, water holding capacity and meat moisture content. Also, at the end of the fifth week, two samples were randomly selected from each replicate and blood was taken from their wing vein to measure blood parameters, thyroid hormones and testosterone.
Results and Discussion: The addition of mint and kefir extract significantly increased the weight of Japanese quails. The highest weight gain in the third and fourth weeks was observed in treatments 2, 3 and 4, respectively. In a study that investigated the effect of kefir on broiler performance, it was shown that the use of kefir as a natural probiotic causes significant weight gain, which is consistent with the present results. Mentha pipperita essential oil and kefir have been reported to improve feed conversion ratio and stimulate digestion in broilers. Kefir added to broiler drinking water has been reported to increase body weight, increase daily weight, reduce daily feed intake and feed conversion ratio, and improve performance. Mentha pipperita lowers blood cholesterol levels by liver or intestinal cells. Peppermint also accelerates LDL catabolism by increasing liver LDL receptors. In addition, Mentha pipperita inhibits the activity of the enzyme fatty acid synthase (FAS) and increases the beta oxidation of fatty acids, thereby effectively reducing fat storage. The concentration of thyroid hormones was not affected by the treatments. In some studies, thyroid hormone concentrations increase with nutrition of mint and kefir. Testosterone concentrations were significantly affected by experimental treatments. Changes in the hypothalamic-pituitary-gonadal axis are the most important factors affecting the reproductive system. So far, the effect of many different plant extracts on the hypothalamic-pituitary-gonadal hormonal axis has been investigated. Plant extracts such as marjoram and ginger have been reported to increase testosterone, LH and sometimes FSH, and play an effective role in activating the hypothalamic-pituitary-gonadal axis. Peppermint, with its special chemical composition, can stimulate the signaling pathways of sex steroid production in the hypothalamic-pituitary-gonadal axis. The effect of experimental treatments on pH and moisture of breast and thigh meat and their water holding capacity was not significant.
Conclusion: According to this study and economic considerations, it seems that the combined use of 2% kefir and 0.5% Mentha pipperita in drinking water can improve the performance and also modulate lipid profiles in Japanese quails, as well as increase reproductive performance in males.

Keywords

• Antioxidant
• Japanese Quail
• Probiotic
• Plasma

20.1001.1.20083106.1401.14.1.7.8

Main Subjects

• Animal Physiology and biotechnology

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References

1. Abbaspour, B., and S. D. Sharifi. 2016. A Study on the Antioxidant and Hypolipidemic Effects of Gracilariopsis Persica Seaweed in Japanese Quail. Research On Animal Production (Scientific and Research), 6(12): 12-19.   doi:sanru.ac.ir/article-1-558-fa.html (In Persian).
2. Akbari, M., and M. Torki. 2014. Effects of dietary chromium picolinate and peppermint essential oil on growth performance and blood biochemical parameters of broiler chicks reared under heat stress conditions. International Journal of Biometeorology, 58(6): 1383-1391. doi: 10.1007/s00484-013-0740-1. ‏
3. Andogan C., B. Hasan, K. Selcuk, D. Mustafa, O. Demir, and M. Ethem. 2002. Antimicrobial activity and chemical composition of some essential oils. Archives of Pharmacal Research, 25 (6): 860–864.
4. Azizi, K., M. Daneshyar, S. M. Abtahi, and S. H. Goldani. 2017. Performance, carcass characteristics and immune response of Japanese quails to different levels of Mentha piperita L. Iranian Journal of Medicinal and Aromatic Plants, 33(5):820-836.‏ doi: 10.22092/IJMAPR.2017.109118.1933 (In Persian)
5. Bandforouzi, K., M. Rezaei, and M. KazemiFard. 2019. Effects of Different Levels of Tomato Powder with and without Addition of Enzymes on Performance, Blood Parameters and Antioxidant Status of Japanese Quails. Research on Animal Production, 10(23):11-21. doi: http://rap.sanru.ac.ir/article-1-888-en.html (In Persian).
6. Beuchat, L. R., and D. A. Golden. 1989. Antimicrobials occurring naturally in foods. Food Technology, 43: 134–142.
7. Castellini, C., C. mugani, and A. Dal Bosco. 2002. Effect of organic production system on broiler carcass and meat quality. Meat Science, 60: 219-225. https://doi.org/10.1016/S0309-1740(01)00124-3.
8. Cenesiz, S., H. A. Yaman, A. Ozcan, A. Kart, and G. Karademir. 2008. Effects of kefir as a probiotic on serum cholesterol, total lipid, aspartate amino transferase and alanine amino trans ferase activities in broiler chicks. Medycyna Weterynaryjna, 64(2):168-170.
9. Cho H., Z. F. Zhang, and I. H. Kim .2013. Effects of single or combined dietary supplementation of b-glucan and kefir on growth performance, blood characteristics and meat quality in broilers. British Poultry Science, 54:216-221.
10. Clegg J., B. Middleton, G. D. Bell, D. With, A. Szabo, and O. Gasic. 1999. Pharmacological studies of Mentha longifolia phenolic extracts. II. Hepatoprotective activity. Pharmaceutical biology, 37: 221-224. https://doi.org/10.1076/phbi.37.3.221.6306.
11. Crossland, J. 1980. Lewiss Pharmacologi, 5th Ed. Churchill. P:656-657.
12. Dagnew, M. B. and Gunther. E. 1990. Epidemiology of communicable skin diseases in school children of a rural area in North Ethiopia. Dermatologische Monatschrift, 176(4): 219-223.‏
13. Dosti, A., K. Taherpour, J. Nasr, and H. Ghasemi. 2014. The comparative effects of dietary peppermint (Mentha pipperita), probiotic and prebiotic on growth performance and serum biochemical parameters of broilers performance. Animal Sciences Journal, 101: 91-101.‏(In Persian)
14. Dransfield, E., and A. A. Sosnicki. 1999. Relationship between muscle growth and poultry meat quality. Poultry Science, 78: 743-746. https://doi.org/10.1093/ps/78.5.743
15. Erdogan, F. S., S. Ozarslan, Z. B. Guzel-Seydim, and T. K. Tas. 2019. The effect of kefir produced from natural kefir grains on the intestinal microbial populations and antioxidant capacities of Balb/c mice. Food Research International, 115: 408-413.‏ https://doi.org/10.1016/j.foodres.2018.10.080.
16. Fuller 1992. Probiotics: Applications and practical aspects. Chapmand and Hall.London.
17. Hafeez , K. Manner, C. Schieder, and J. Zentek. 2015. Effect of supplementation of phytogenic feed additives (powdered vs. encapsulated) on performance and nutrient digestibility in broiler chickens. Poultry Science, 95 (3): 622–629. https://doi.org/10.3382/ps/pev368.
18. Hemayatkhah Jahromi V., K. Parivar, and M. Forozanfar, 2011. The Effect of Cinnamon Extract on Spermatogenesis Hormonal Axis of Pituitary Gonad in Mice. Iranian Journal of Applied Animal Science, 1 (2): 99-103.
19. Kafshdouzan, K., B. Rouzbehan, and M. Moslemy. 2013. Reviewing the role of probiotics used in poultry feeding on health promotion of chicken meet. Iranian Journal of Nutrition Sciences and Food Technology, 7(5): 821-828.‏ http://nsft.sbmu.ac.ir/article-1-1083-en.html (In Persian).
20. Kazemi, P., H. Jowhary, E. Sharifi, and A. Zeraatpishe. 2012. Androgenic effect of origanum vulgarel l. spp viride extract on hormone level of pituitary-gonadal axis in mature male vistar rats. Journal of Arak University of Medical Sciences, 14(6): 89-96.‏ http://jams.arakmu.ac.ir/article-1-794-en.html (In Persian).
21. Kotula, K. L. and Y. Wang. 1994. Characterization of broiler meat quality factors as influenced by feed withdrawal time. Journal of Applied Poultry Research, 3: 103-110.
22. Lahucky, R., I. Bahelka, K. Novotna, and K. Vasickova, 2005. Effects of dietary vitamin E and vitamin C supplementation on the level of α-tocopherol and L-ascorbic acid in muscle and on the antioxidative status and meat quality of pigs. Czech Journal of Animal Science, 50: 175-184.
23. Lovkova Y., Buzuk, G. N. Sokolova S. M.  and Klimenteva. N. I. 2001. Chemical features of medicinal plants. Applied Biochemistry and Microbiology, 37 (3): 229–237.
24. Moravej, H. 2008. Poultry behaviour and welfare. University of Tehran Press. Vol. 3.  978-964-03-5852-8.
25. Puerari, C., K. T. Magalhaes, and R. F. Schwan. 2012. New cocoa pulp-based kefir beverages: Microbiological, chemical composition and sensory analysis. Food Research International, 48 (2): 634–640. https://doi.org/10.1016/j.foodres.2012.06.005.
26. Purutoglu, K., H. Ispirli, M. O.Yuzer, H. Serencam, and E. Dertli. 2019. Diversity and functional characteristics of lactic acid bacteria from traditional kefir grains. International Journal of Dairy Technology, 70:1– https://doi.org/10.1111/1471-0307.12633.
27. Salma , A. G. Miah, T. Maki, M. Nishimura, and H. Tsujii. 2007. Effect of dietary Rhodobacter capsulatus on cholesterol concentration and fatty acid composition in broiler meat. Poultry Science, 86(9): 1920-1926.‏ https://doi.org/10.1093/ps/86.9.1920.
28. Sharifi, M., A. Moridnia, D. Mortazavi, M. Salehi, M. Bagheri and A. Sheikhi. 2017. Kefir: A powerful probiotics with anticancer properties. Medical Oncology, 34(183): 1– doi: 10.1007/s12032-017-1044-9.
29. Taghavi, N., L. Nateghi, and S. Berenji. 2018. Feasibility of functional ice cream on the basis of green coffee fiber and kefir seeds. Journal of Food Research, 28(2): 161-177. (In Persian)
30. Taherpour , H. Moravej, M. Shivazad, M. Adibmoradi, and B. Yakhchali. 2009. Effects of dietary probiotic, prebiotic and butyric acid glycerides on performance and serum composition in broiler chickens. African Journal of Biotechnology, 8 (10): 2329-2334.
31. Toghyani , M. S. kazem, M. Modaresi, and N. Landy. 2015. Evaluation of kefir as a potential probiotic on growth performance, serum biochemistry and immune responses in broiler chicks. Animal Nutrition, 1(4): 305-309.‏ https://doi.org/10.1016/j.aninu.2015.11.010.
32. Vahdatpour, T., and D. Babazadeh. 2016. The effects of kefir rich in probiotic administration on serum enzymes and performance in male Japanese quails. Journal of Animal and Plant Science, 26: 34–39.
33. Van der Zijpp, A. J. 1999. Animal food production: the perspective of human consumption, production, trade and disease control. Livestock Production Science, 59 (2-3): 199–206. https://doi.org/10.1016/S0301-6226(99)00027-5.
34. Vimercati, W. C., C. Silva Araujo, L. L. Macedo, H. C. Fonseca, J. S. Guimaraes, L. R. de Abreu, and S. M. Pinto. 2020. Physicochemical, rheological, microbiological and sensory properties of newly developed coffee flavored kefir. LWT - Food Science and Technology, https://doi.org/10.1016/j.lwt.2020.109069.‏
35. Yaman , Z. Ulukanli, M. Elmali, and Y. Unal .2006. The effect of a fermented probiotic, the kefir, on intestinal flora of poultry domesticated geese. Health & Environmental Research Online (HERO), 157 (7): 379–38.
36. Zubair K., and S. Leeson. 1996. Compensatory growth in the broiler chicken: a review. Worlds Poultry Science Journal, 52: 189–201. https://doi.org/10.1079/WPS19960015.