نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه علوم دامی، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران
2 آموزشکده کشاورزی سرایان، دانشگاه بیرجند، بیرجند، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Fungal toxins are also of considerable importance and are always placed next to plant pesticides and heavy metals. Mycotoxins are small molecules that are produced as secondary metabolites by filamentous fungi and yeasts. Aflatoxins, secondary metabolites of various Aspergillus spp, commonly contaminate a wide variety of tropical and subtropical food/feed stuffs. Chemically, aflatoxins are difuranocoumarin compounds and include B1, B2, G1, G2, M1, and M2. The removal of aflatoxins from contaminated food is a major problem in livestock and poultry nutrition and the pollution removal methods are based on the decomposition, destruction, inactivation or removal of aflatoxins through biological, chemical or physical methods. In recent years, the study of the effect of plants and their extracts and compounds on things such as reducing microbial growth and the effect on microorganisms has increased dramatically. The aim of this research is to investigate the effect of the combination of garlic and turmeric extract on the amount of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, the amount of triiodothyronine, thyroxine, total antioxidant capacity and malondialdehyde. in rats infected with aflatoxin B1. Materials and methods In this research, 64 male Wistar rats with an average body weight of 250-300 gr were used to evaluate the synergistic effect of garlic and turmeric extract against the damage caused by Aflatoxin B1 in the form of a completely randomized design as a factorial experiment (2×2 2) Eight groups were tested for 28 days in the animal dissection laboratory of the Faculty of Agriculture of Birjand University, observing all ethical considerations.This study aimed to determine the synergistic effect of Garlic and Turmeric extracts for possible protection against injury induced by Aflatoxin B1 (AFB1). 64 male rats were divided into eight groups and treated for 28 days including control (0.2 ml Sterile distilled water orally), AFB1 (100 µg/kg b.w orally), Garlic extract (GE) (50 mg/kg b.w orally), Turmeric extract (TE) (100 mg/kg b.w orally), GE plus TE, AFB1 plus GE, AFB1 plus TE, AFB1 plus GE and TE. At the end of the treatment period, blood samples were collected for biochemical study. Results and discussion The use of Aflatoxin B1 at the rate of 100 mg/kg of body weight daily for 28 days increased AST, ALT, ALP, total bilirubin, malondialdehyde and decreased total antioxidant capacity, T3 and T4. The extract of garlic, turmeric and their combination had a positive effect on poisoning caused by aflatoxin B1 and caused a significant decrease in AST, ALP, total bilirubin, malondialdehyde and a significant increase in total antioxidant capacity and It was T3. The results showed that the GE and/or TE had no significant effect on measured parameters. Aflatoxin B1 significantly increased AST, ALT, ALP, total bilirubin, malondialdehyde and significantly decreased total antioxidant capacity, T3 (triiodothyronine) and T4 (thyroxin). Assessment of data shows that GE and TE have potential effect on decreasing levels of ALP, ALT and increasing level of T4 comparing to contaminated groups receiving GE or TE alone. A significant increase in T4 and a significant decrease in ALT were observed only in the group treated with the combination of garlic and turmeric extract compared to the aflatoxin B1 control. In examining the ALP factor, there was a statistically significant difference between the treatments of garlic or turmeric extract and the combination of these two extracts. One of the possible protective mechanisms of these plants is their antioxidant property, which prevents the activity of free radicals produced by Aflatoxin B1. Conclusion The results of the present study indicate that the use of garlic extract at the rate of 50 mg/kg of body weight, turmeric extract at the rate of 100 mg/kg of body weight, and their combination reduces the level of toxicity. caused by aflatoxin B1 reduces. The use of garlic, turmeric extract and their combination improves liver function indicators and reduces liver enzymes such as AST, ALT, ALP and total bilirubin. These results show that the extract of garlic, turmeric and their combination have a protective effect on the liver. The use of turmeric extract, garlic and the combination of garlic and turmeric extract alone had no effect on the measured variables. In general, the combination of GE and TE may be have a synergistic effect in reduce the adverse effects of AFB1.
کلیدواژهها [English]
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