تأثیر مقادیر مختلف نانو ذرات نقره سنتز شده به روش زیستی از عصاره گیاه گوش بره سفید (Phlomis cancellata Bunge) بر تخمیر شکمبه‌ای در شرایط برون‌تنی

نوع مقاله : علمی پژوهشی - تغذیه نشخوارکنندگان

نویسندگان

1 گروه علوم‌دامی، مجتمع آموزش‌ عالی تربت‌جام، تربت‌جام، ایران.

2 گروه شیمی، مجتمع آموزش‌ عالی تربت‌جام، تربت‌جام، ایران

چکیده

این آزمایش با هدف بررسی اثر مقادیر مختلف نانو ذرات نقره سنتز شده به روش زیستی از عصاره گیاه گوش‌بره​ سفید بر تخمیر شکمبه‌ای در شرایط برون‌تنی انجام شد. جهت تهیه عصاره گیاه گوش‌بره سفید، یک میلی‌لیتر از عصاره تهیه‌شده از اندام‌های هوایی گیاه به محلول نیترات نقره 76/4 میلی‌مولار در 84/6 pH= اضافه گردید و به مدت 25 دقیقه در دمای 77 درجه سلسیوس مورد انکوباسیون قرار گرفت. اثرات افزایش نانو ذرات سنتز شده از گیاه گوش‌بره سفید (0، 125 و 250 میکروگرم در میلی‌لیتر) بر خصوصیات تخمیری جیره گاوهای پرتولید با استفاده از روش تولید گاز در قالب طرح کاملاً تصادفی مورد ارزیابی قرار گرفت. افزایش مقدار نانو ذرات نقره در تیمار‌های آزمایشی تأثیر معنی‌داری بر ظرفیت تولید گاز از بخش قابل تخمیر، نرخ تولید گاز و حجم گاز تجمعی تولیدی (میلی‌لیتر/میلی‌گرم ماده خشک) در ساعات مختلف انکوباسیون نداشت. در این آزمایش بین تیمارهای مختلف از نظر قابلیت‌هضم ظاهری ماده خشک، ماده آلی، مقدار ماده آلی هضم‌شده واقعی، انرژی قابل‌‌متابولیسم، اسیدهای چرب فرار، شاخص بخش‌پذیری، تولید توده میکروبی و بازده آن اختلاف معنی‌داری وجود نداشت. در تیمار یک (فاقد نانو ذرات نقره سنتز شده به روش زیستی از عصاره گیاه گوش‌بره​ سفید) همبستگی منفی و معنی‌دار بین هضم ماده خشک، قابلیت‌هضم ماده آلی با حجم گاز تجمعی تولیدی در ساعات 12 و 24 انکوباسیون و در تیمار دو (حاوی 125 میکروگرم در میلی‌لیتر نانو ذرات نقره سنتز شده به روش زیستی از عصاره گیاه گوش‌بره​ سفید) همبستگی منفی و معنی‌دار بین قابلیت‌هضم ماده آلی، شاخص بخش‌پذیری با حجم گاز تجمعی تولیدی در ساعات مختلف انکوباسیون ثبت گردید. مکمل‌ کردن مقادیر افزایشی نانو ذرات نقره توانست فراسنجه‌های تولید گاز در خوراک پایه را تغییر دهد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of Green Silver Nanoparticles Synthesized via Phlomis cancellata Bunge Extract on in vitro Ruminal Fermentation

نویسندگان [English]

  • Elias Ibrahimi Khoram Abadi 1
  • Mohsen Kazemi 1
  • Somayeh Heydari 2
1 Department of Animal Sciences, Faculty of Agriculture and Animal Science, University of Torbat-e Jam, Torbat-e Jam, Iran.
2 Department of Chemistry, Faculty of Agriculture and Animal Science, University of Torbat-e Jam, Torbat-e Jam, Iran.
چکیده [English]

Introduction[1] The anaerobic microbial fermentative digestion of feedstuffs in the rumen is not efficient. The gases are considered as waste products of rumen fermentation and also pollutants of the environment. Recent studies indicated that some metal nanoparticles (NPs) were toxic to rumen microbial population and inhibit methane production in anaerobic conditions. Plant extracts can be used to produce cost effective and eco-friendly green nanoparticles. Phlomis cancellata Bunge with Persian names Gushbarre sefid has high medicinal value and antibacterial properties and distributed dramatically in Khorasan, Mazandaran and Golestan. Hence, there is potential of using bio-synthesized nanoparticles in ruminant nutrition. However, there is not enough information regarding the effect of green nanoparticles on runminal condition. Therefore, the present investigation was carried out to study the effect of green silver nanoparticles synthesized via Phlomis cancellata Bunge extract on rumen fermentation in vitro.
Materials and methods Synthesis of sliver nanoparticles was prepared by adding of 1 ml of the aqueous extract to 4.76 mM sliver nitrate solution (pH= 6.84) allowed to react at 77 °C for 24.79 min in the dark to minimize the photo activation of silver nitrate. The color change of solution from yellow to brown after 3 min of incubation is indicative of the bioreduction of Ag+ ions in the solution to Ag°. Effects of increasing the concentration of green silver nanoparticles synthesized via Phlomis cancellata Bunge extract (0, 125 and 250 µg/ml) on rumen fermentation were evaluated using in vitro gas production technique. Gas production volumes were recorded at 3, 6, 9, 12, 24, 48, 72 and 96 h of incubation and then gas production kinetic was estimated. The obtained data from gas production at 24 h after incubation were used for estimation of digestible dry and organic matter, metabolisable energy, short chain fatty acids, partitioning factor, microbial biomass production and microbial biomass production efficiency.
Results and Discussion The increasing level of green silver nanoparticles synthesized via Phlomis cancellata Bunge extract did not significantly affect in vitro potential of gas production and gas production rate. For the cumulative gas production, no significant difference was found among the treatments. The lowest and highest in vitro potential of gas production, gas production rate and cumulative gas production was recorded for treatments 3 and 1, respectively. Silver nanoparticles exhibit unique bacteriostatic and bactericidal properties. At the atomic level, silver has the ability to absorb oxygen and acts as an oxidation catalyst. Atomic oxygen absorbed on the silver surface reacts with the thiol groups surrounding the surface of bacteria and viruses and removes hydrogen atoms. The bacterium loses respiration ability by disruption of the so-called respiratory channel, which results in bacterial death. The apparent in vitro dry matter digestibility and organic matter digestibility and true in vitro organic matter digestibility, were not significantly affected by addition of green silver nanoparticles synthesized via Phlomis cancellata Bunge extract. Addition of green silver nanoparticles synthesized via Phlomis cancellata Bunge extract failed to affect metabolisable energy, short chain fatty acids, partitioning factor, microbial biomass production and microbial biomass production efficiency. However, the lack of significant effect of the synthesized silver nanoparticles on the digestibility may be due to factors such as concentration, surface capacity, size, and other properties of silver nanoparticles. For treatment 1, the cumulative gas production over 24 and 48 h was negatively correlated apparent in vitro dry matter digestibility and organic matter digestibility. There was significant negative correlation between apparent in vitro organic matter digestibility and partitioning factor with cumulative gas production during hours of incubation for treatments 2. No significant correlation was found between apparent in vitro dry matter digestibility and organic matter digestibility and partitioning factor with the volume of cumulative gas produced during different incubation hours in for treatments 3.
Conclusion Supplementing of green silver nanoparticles synthesized via Phlomis cancellata Bunge extract could modify the characteristics of gas production and fermentation parameters basal diet and reduce the side effects of the anaerobic microbial fermentation. However, additional microbial studies with different level of green silver nanoparticles are necessary to determine the mode of action. Additionally, further in vivo work is needed to assess the effect of green silver nanoparticles inclusion on animal performance when cattle are fed ingredients commonly used in beef feedlot or dairy diets.

کلیدواژه‌ها [English]

  • Diet
  • Gas production
  • Phlomis cancellata Bunge
  • rumen
  • Silver nanoparticles
  1. Abd El-Galil, E. R. I., and N. E. Y. El-Bordeny. 2018. Evaluation of nanocobalt particles addition in ruminant rations by in vitro gas production. Egyptian Journal of Nutrition and Feeds, 21(1):91-102.
  2. Akhlaghi, H., and A. Motevalizadeh Kakh’ky. 2010. Volatile Constituents of Phlomis cancellata Bunge. A Labiate Herb Indigenous in Iran Journal of Essential Oil Research, 13(5):134-137. (In Persian).
  3. Arabi, F., M. Imandar, M. Negahdary, M. Imandar, M. T. Noughabi, H. Akbari-dastjerdi, and M. Fazilati. 2012. Investigation anti-bacterial effect of zinc oxide nanoparticles upon life of Listeria monocytogenes. Annals of Biological Research, 7:3679-3685. (In Persian)
  4. Bellamy, D., and A. Pfister. 1992. World medicine: plants, patients and people. Oxford: Blackwell Publishers, 1321p.
  5. Benchaar, C., S. Calsamiglia, A. V. Chaves, G. R. Fraser, D. Colombatto, A. McAllister, and K. A. Beauchemin. 2008. A review of plant-derived essential oils in ruminant nutrition and production. Animal Feed Science and Technology, 145:209-228.
  6. Blummel, M., and E. R. 1993. Composition of in vitro gas production and nylon bag degradability of roughages in predicting food intake in cattle. Animal Feed Science and Technology, 40:109-119.
  7. Buxton, D. R., and D. D. Redfearn. 1997. Plant limitations to fiber digestion and utilization. The Journal of Nutrition, 127(5):814-818.
  8. Castro-Montoyaa, J., S. De Campeneere. G. Van Ranst, and V. Fievez. 2012. Interactions between methane mitigation additives and basal substrates on in vitro methane and VFA production. Animal Feed Science and Technology, 176:47-60.
  9. Cui, L., P. Chen, S. Chen, Z. Yuan, C. Yu, B. Ren, and K. Zhang. 2013. In situ study of the antibacterial activity and mechanism of action of silver nanoparticles by surface-enhanced Raman spectroscopy. Analytical Chemistry, 85:5436-5443.
  10. Dehority, B. A. 2003. Rumen Microbiology. Nottingham University Press, Nottingham, UK.
  11. Deylamsalehi, M., M. Mahdavi, A. Motavalizadehkakhky, M. Akbarzadeh, J. Mahmudi, S. F. Mirahmadi, Z. Ebrahimi, and F. Abedi. 2013. Chemical compositions and antimicrobial activity of essential oil of Phlomis cancellata Bunge. From Mazandaran. Tropical Journal of Pharmaceutical Research, 16(4):555-562. (In Persian).
  12. Fondevila, M. 2010. Handbook of Potential use of silver nanoparticles as an additive in animal feeding, 325-334.
  13. Formisano, C., F. Senatore, M. Bruno, and G. Bellone. 2006. Chemical composition and antimicrobial activity of the essential oil of Phlomis ferruginea ten. Growing wild in Southern Italy. Flavour and Fragrance Journal, 21:848-851.
  14. García-González, R., S. López, M. Fernández, and J. S. González. 2006. Effects of the addition of some medicinal plants on methane production in a rumen simulating fermenter (RUSITEC). International Congress Series, 1293:172-175.
  15. Getachew, G., H. P. S. Makkar, and K. Becker. 2000. Effect of polyethylene glycol on in vitro degradability of nitrogen and microbial protein synthesis from tannin-rich browse and herbaceous legumes. British Journal of Nutrition, 84:73-83.
  16. Getachew, G., H. P. S. Makkar, and K. Becker. 2002. Tropical browses: content of phenolic compounds,in vitro gas production and stoichiometric relationship between short chain fatty acids and invitro gas production. Journal of Agricultural Science, 139:341-352.
  17. Gonzalez-Estrella, J., R. Sierra-Alvarez, and J. A. Field. 2013. Toxicity assessment of inorganic nanoparticles to acetoclastic and hydrogenotrophic methanogenic activity in anaerobic granular sludge. Journal of Hazardous Materials, 260:278-285.
  18. Hartemann, P., P. Hoet, A. Proykova, T. Fernandes, A. Baun, W. De Jong, J. Filser, A. Hensten, K. Kneuer, J. V. Maillard, H. Norppa, M. Scheringer, and S. Wijnhoven. 2015. Nanosilver: safety, health and environmental effects and role in antimicrobial resistance. Materials Today, 18:122-123.
  19. Heidari, S., and M. Hosseinpour Zaryabi. 2018. Response Surface Methodology for Optimization of Green Silver Nanoparticles Synthesized via Phlomis Cancellata Bunge Extract. Analytical and Bioanalytical Chemistry Research, 5(2):373-386.
  20. Hino, T., and N. Asanuma. 2003. Suppression of ruminal methanogenesis by decreasing the substrates available to methanogenic bacteria. Nutrition Abstract and Reviews (Series B), 73:1-8.
  21. Ipharraguerre, I. R., and J. H. Clark. 2003. Usefulness of ionophores for lactating dairy cows: A review. Animal Feed Science and Technology, 106:39-57.
  22. Kavitha, K. S., S. Baker, D. Rakshith, H. U. Kavitha, H. C. Yashwantha Rao, B. P. Harini, and S. Satish. 2013. Plants as green source towards synthesis of nanoparticles. International Research Journal of Biological Sciences, 2(6):66-76.
  23. Khafipour, E., D. O. Krause, and J. C. Plaizier. 2009. Alfalfa pellet-induced subacute ruminal acidosis in dairy cows increases bacterial endotoxin in the rumen without causing inflammation. Journal of Dairy Science, 92:1712-1724. (In Persian).
  24. Khalilzadeh, M., A. Rustaiyan, S. Masoudi, and M. Tajbakhsh. 2005. Essential oils of Phlomis persica Boiss. and Phlomis olivieri Benth. from Iran. Journal of Essential Oil Research, 17(6):624-625. (In Persian).
  25. Kirimer, N., K. Baser, and M. Kurkcuoglu. 2006. Composition of the Essential Oil of Phlomis nissolii L. Journal of Essential Oil Research, 12:12-16.
  26. Lara, H. H., N. V. Ayala-Nunez, L. C. I. Turrent, and C. R. Padilla. 2009. Bactericidal effect of silver nanoparticles against multidrug-resistant bacteria. World Journal of Microbiology and Biotechnology, 26:615-621.
  27. Li, B. T., A. G. Van Kessel, W. R. Caine, S. X. Huang, and R. N. Kirkwood. 2001. Small intestinal morphology and bacterial populations in ileal digesta and feces of newly weaned pigs receiving a high dietary level of zinc oxide. Canadian Journal of Animal Science, 81:511-516.
  28. Li, W. R., X. B. Xie, Q. S. Shi, S. S. Duan, Y. S. Ouyang, and Y. B. Chen. 2011. Antibacterial effect of silver nanoparticles on Staphylococcus aureus. Biometals, 24:135-141.
  29. Luna-delRisco, M., K. Orupõld, and H. C. Dubourguier. 2011 Particle-size effect of CuO and ZnO on biogas and methane production during anaerobic digestion. Journal of Hazardous Materials, 189(1):603-608.
  30. Makkar, H. P. S. 2005. In vitro gas methods for evaluation of feeds containing phytochemicals. Animal Feed Science and Technology, 123:291-302.
  31. Masoudi, S. H., A. Rustaiyan, P. Azar, and K. Larijani. 2006. Composition of the Essential Oils of Cyclotrichium straussii and Phlomis pungens Willd. from Iran. Journal of Essential Oil Research, 34(5):134-137.
  32. McGuffey, R. K., L. F. Richardson, and J. I. D. Wilkinson. 2001. Ionophores for dairy cattle: current status and future outlook. Journal of Dairy Science, 84(E. Suppl.): E194-E203.
  33. McSweeney, C., and R. Mackie. 2012. Micro-organisms and ruminant digestion: State of knowledge, trends and future prospects. Commission on Genetic Resources for Food and Agriculture, Food and Agriculture Organization of United Nations, Rome, Italy, Background study -61.
  34. Menke, K. H., L. A. Salewski, H. Steingass, D. Fritz, and W. Schneider. 1979. The estimation of the digestibility and metabolisable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor. The Journal of Agricultural Science, 93:217-222.
  35. Menke, K. H., and H. Steingass. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal research and development, 28:7-55.
  36. Mittal, A. K., Y. Chisti, and U. C. Banerjee. 2013. Synthesis of metallic nanoparticles using plant extracts. Biotechnology Advances, 31(2):346-56.
  37. Morteza-Semnani, K., K. Moshiri, and M. Akbarzadeh. 2006. Essential oil composition of Phlomis cancellata Bunge. Journal of Essential Oil Research, 18(6):672-673. (In Persian).
  38. Mortimer, M., K. Kasemets, and A. Kahru. 2010. Toxicity of ZnO and CuO nanoparticles to ciliated protozoa Tetrahymena thermophila. Toxicology, 269(2):182-189.
  39. Noeck, J. E. 1988. In situ and other methods to estimate ruminal protein and energy digestibility: A Review. Journal of Dairy Science, 71:2051-2069.
  40. Ǿrskov, E. R., and I. McDonald. 1979. The estimation of protein degradability in the rumen from incubation measurements Weighed according to the rate of passage. The Journal of Agricultural Science, 92:499-503.
  41. Parnian Khaje Dizaj, F., A. Taghizadeh, G. A. Moghaddam, and H. Janmohammadi. 2010. Use of in Vitro Gas Production Technique for Evaluation of Nutritive Parameters of Barley and Corn Grain Treated by Different Microwave Irradiation Times. Journal of Animal Science Research, 21(1):16-27. (In Persian).
  42. Rajendran, R. 2013. Application of nano minerals in animal production system.Research. Journal of Biotechnology, 8(3):1-3.
  43. Rajeshkumar, S., C. Malarkodi, G. Gnanajobitha, K. Paulkumar, M. Vanaja, C. Kannan, and G. Annadurai. 2013. Seaweed-mediated synthesis of gold nanoparticles using Turbinaria conoides and its characterization. Journal of Nanostructure in Chemistry, 3:44-50.
  44. Sadeghi, B., A. Rostami, and S. S. Momei. 2015. Facile Green Synthesis of Silver Nanoparticles Using Seed Aqueous Extract of Pistacia atlantica and Its Antibacterial Activity. Spectrochimica Acta Part A: Molecular and Bimolecular Spectroscopy, 134:326-332. (In Persian).
  45. Sai Ram Kumar, S. 2017. Green synthesis of nanoparticles using plant extracts and their effect on rumen fermentation in vitro. Thesis.
  46. Sarker, N. C., F. Keomanivong, M. D. Borhan, S. Rahman, and K. Swanson. 2018. In vitro evaluation of nano zinc oxide (nZnO) on mitigation of gaseous emissions. Journal of Animal Science and Technology, 60:27.
  47. Sarkhail, P., G. Amin, M. Surmaghi, and A. Shafiee. 2005. Composition of the volatile oils of Phlomis lanceolata Boiss. & Hohen., Phlomis anisodonta Boiss. and Phlomis bruguieri Desf. from Iran. Flavor and Fragrance Journal, 20:327-329.
  48. Senapati, S., A. Syde, S. Moeez, A. Kumar, and A. Ahmah. 2012. Intracellular synthesis of gold nanoparticles using alga Tetraselmis kochinensis. Material Letters, 2:275-281.
  49. Shi, L., W. Xun, W. Yue, C. Zhang, Y. Ren, Q. Liu, Q. Wang, and L. Shi. 2011(a). Effect of elemental nano-selenium on feed digestibility, rumen fermentation, and purine derivatives in sheep. Animal Feed Science and Technology, 163(2):136-142.
  50. Shi L., R. J. Yang, W. B. Yue, W. J. Xun, C. X. Zhang, Y. S. Ren, L. Shi, and F. L. Lei. 2010. Effect of elemental nano-selenium on semen quality, glutathione peroxidase activity, and testis ultrastructure in male Boer goats. Animal Reproduction Science, 118(2):248-254.
  51. Theodorou, M. K., B. A. Williams, M. S. Dhanoa, A. B. McAllan, and J. France. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology, 48:185-197.
  52. Tomkins, N. W., S. E. Denmanb, P. Pilajunc, M. Wanapatc, C. S. McSweeney, and R. Elliot. 2015. Manipulating rumen fermentation and methanogenesis using an essential oil and monensinin beef cattle feda tropical grasshay. Animal Feed Science and Technology, 200:25-34.
  53. Xun, W., L. Shi, W. Yue, C. Zhang, Y. Ren, and Q. Liu. 2012. Effect of high-dose nano selenium and selenium–yeast on feed digestibility, rumen fermentation, and purine derivatives in sheep. Biological Trace Element Research, 150(1-3):130-136.
  54. Yang, W., C. Shen, Q. Ji, A. H. Wang, J. Q. Liu, and Z. Zhang. 2009. Food storage material silver nanoparticles interfere with DNA replication fidelity and bind with DNA. Nanotechnology, 2:2121-2134.
  55. Yang, Z. P., and L. P. Sun. 2006. Effects of nanometer ZnO on growth performance of early weaned piglets. Journal of Shanxi Agriculture Science, 3:024.
  56. Yoon, K. Y., J. H. byeon, J. H. Park, and J. Hwang. 2007. Susceptibility contrants of Eschericia coli and Bacillus subtillis to silver and copper nanoparticles. Science of the Total Environment, 373:572-575.
  57. Zhisheng, C. J. 2011. Effect of nano-zinc oxide supplementation on rumen fermentation in vitro. Chinese Journal of Animal Nutrition, 8:023.