تاثیر اشکال مختلف سلنیوم بر فراسنجه های تولید گاز، تخمیر شکمبه و جمعیت پروتوزوآی شکمبه

نوع مقاله : علمی پژوهشی - تغذیه نشخوارکنندگان

نویسندگان

1 دانشگاه تبریز

2 گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز

چکیده

تحقیق حاضر به منظور ارزیابی اثر اشکال مختلف سلنیوم بر تولید گاز، تخمیر شکمبه و جمعیت پروتوزوآی شکمبه گوسفندان قزل گرفت. تیمارهای آزمایشی شامل 1. کنسانتره بدون مکمل سلنیوم 2. کنسانتره +ppm 3/0 نانوسلنیوم 3. کنسانتره + ppm 3/0 سلنیوم آلی و 4. کنسانتره + ppm 3/0 سلنیوم معدنی بودند. در روش تولید­گاز 300 میلی­گرم از هر نمونه در ساعات 2، 4، 6، 8، 12، 16، 24، 36، 48، 72 و 96 انکوباسیون گردید. به منظور بررسی تاثیر اشکال مختلف سلنیوم بر اکوسیستم شکمبه، فاکتورهای pH، اسیدهای­چرب فرار، نیتروژن آمونیاکی و جمعیت پروتوزوآیی مورد بررسی قرار گرفت. از ساعت 36 انکوباسیون به بعد میزان تولید گاز تیمارهای حاوی سلنیوم به طور معنی داری بیشتر از تیمار کنترل بود، اما تفاوت معنی داری از نظر تولید گاز بین این 3 تیمار وجود نداشت. پتانسیل تولید گاز در تیمارهای حاوی سلنیوم از تیمار کنترل بیشتر بوده (به ترتیب 9/201، 0/344، 7/321 و 8/319 میلی لیتر در تیمارهای شاهد، سلنیوم نانو، آلی و معدنی) ولی تفاوت معنی داری بین تیمارهای حاوی انواع مختلف سلنیوم وجود نداشت. تفاوت معنی­داری بین تیمارها از نظر pH شکمبه وجود نداشت. تیمار نانو­ به طور معنی­داری موجب افزایش غلظت اسیدهای چرب فرار نسبت به تیمارهای آلی و معدنی شد (به ترتیب 83/109، 00/98 و 83/89 میلی مول بر لیتر در تیمارهای سلنیوم نانو، آلی و معدنی). تفاوت معنی­داری بین تیمارها از نظر میزان نیتروژن آمونیاکی شکمبه وجود نداشت. تیمار سلنیوم آلی موجب افزایش معنی­دار تعداد کل پروتووزآ نسبت به تیمار سلنیوم معدنی و نانو سلنیوم شد. نتایج تحقیق نشان داد که افزودن مکمل سلنیوم خصوصا به شکل نانو نسبت به فرم رایج معدنی آن، احتمالا از طریق بهبود فرآیند تخمیر در محیط شکمبه، موجب افزایش پتانسیل تولید گاز شد.

عنوان مقاله [English]

The Effects of Different Forms of Selenium on Gas Production Parameters, Rumen Fermentation and Rumen Protozoa Population

نویسندگان [English]

  • Smira Dehghani 1
  • Akbar Taghizadeh 2
  • Hamid Mohammadzadeh 1
1 Universitry of Tabriz
2 Department of Animal Science, Faculty of Agriculture, Tabriz University, Iran.
چکیده [English]

Introduction:
Generally, most feeds used in livestock nutrition are deficient in some nutrients, and require nutritional supplements. Among the supplements, micro and macro minerals are particular importance. Selenium (Se) plays an important role in the reproductive function and immune system and is known as an antioxidant and catalyst for the production of thyroid hormone. It is believed that low selenium absorption in ruminants is due to the deficiency of selenium in ration and its conversion into insoluble form. Nano-particles are smaller and more active than larger particles. The importance of Selenium for rumen microorganisms are not entirely clear. Also, selenium is an essential trace element, and its importance for animal health and productivity has been well confirmed. Selenium has known to be involved in enzyme activity and preventing oxidative damage to body tissue.  Selenium plays important roles in antioxidant systems, prevents cell damage and is necessary for growth, fertility, and immune system in farm animals. Recently, nano -elemental Se has attracted wide spread attention due to its high bioavailability and low toxicity, because nanometer particulates exhibit novel characteristics, such as great specific surface area, high surface activity, a lot of surface active centers, high catalytic efficiency and strong adsorbing ability and over and above the character of low toxicity of Se0. Dietary selenium is an essential trace element for animals and humans with a variety of biological functions. It plays important roles in the regulation of thyroid hormone metabolism, cell growth and antioxidant systems thus, together with alpha-tocopherol prevents cells against oxidative stress damage, also these compounds are necessary for growth, fertility, and immune system health in animals and humans. The objective of this research was to investigation the effects of different sources of selenium on digestion characteristics of concentrate mixture of diets in high producing lactating dairy cows using in vitro gas production technique.
 
Materials and Methods
Four male ruminally fistulated sheep, average 43±4.8 kg of BW, were used in a replicated 4×4 Latin square experiment. Sheep were fed twice daily (08:00 and 18:00 h) at maintenance nutrition requirements with a basal diet consisting of 400 g/kg (dry matter) DM of basal concentrates and 600 g/kg DM of forage. Sheep were placed in metabolic cages individually and fresh water was freely available during the experimental period. This experiment was conducted in four periods of 28 days with 21 d adaptation period and 7 d for data tacking. Treatments were: 1. Basal diet 2. Basal diet + 0.3 ppm nano selenium, 3. Basal diet + 0.3 ppm seleno methionine, 4. Basal diet + 0.3 ppm selenite sodium. The rumen fluid was mixed with artificial saliva (1:2 ratio, respectively) in lab, and then nano-Selevels and seleno methionine added to it. In gas production method, 300 mg of each treatment weighted and incubated for 2, 4, 6, 8, 12, 16, 24, 36, 48, 72, 96 hours.  In order to determine the effects of different forms of selenium on rumen parameters, concentrations of VFA and NH4, value of pH and population of protozoa were examined. Samples of rumen fluid were collected through the cannula at 4 h after feeding on days 19 and 20 of each collection period for pH, NH3-N, and volatile fatty acids (VFAs) determination. Ruminal pH was immediately measured using an electric pH meter. The samples were subsequently stored frozen at −20 ◦C until analyses.
Results and Discussion:
 Potential of gas production (fraction A) of nano, organic and inorganic treatments were higher (201.9, 344.0, 321.7 and 319.8 ml in control, nano, organic and mineral treatments, respectively) compared to control treatment (P<0.05). The nano selenium treatment had higher VFA concentration (109.83, 98.00 and 89.83 mmol/l in nano, organic and mineral treatments, respectively) when compared with organic and inorganic treatments (P<0.05). Rumen NH4 concentration was not effected by treatments. The organic treatment caused a significant increase in total protozoa population when compared with nano and inorganic treatments (P<0.05).
Conclusion:
 The results indicated that selenium supplementation in ruminant diet improves ruminal nutrients degradability's compared to control. Therefore, the use of nano-Se resulted to increase digestibility and fermentation of nutrients resulted improved rumen microorganisms activities. Although nano and organic selenium was better than inorganic treatment in ruminal degradability and rumen parameters, however there was not any significant differences between these two treatments.

کلیدواژه‌ها [English]

  • Selenium
  • Gas production
  • Dairy cow
  • Protozoa
  • VFA
  • NH4
1. AbdElGhany, H, and J. L. Tortora-Perez. 2010. The importance of selenium and the effects of its deficiency in animal health. Small Ruminant Research, 89: 185-192.
2. AOAC. 2002. Official Methods of Analysis. 17th Edn. AOAC International, Gaithersburg, Maryland, USA.
3. Barceloux, D. G. 1999. Selenium. Journal of Clinical Toxicology, 37: 145-172.
4. Blummel, M, and E. R. Ørskov. 1993. Comparison of gas production and nylon bag degradability of roughages in predicting feed intake in cattle. Animal Feed Science and Technology, 40: 109–119.
5. Broderick, G. A, and J. H. Kang. 1980. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media1. Journal of Dairy Science, 63(1): 64-75.
6. Faixova, Z., S. Faix, L. Leng, P. Vaczi, Z. Makova, and R. Szaboova. 2007. Hematological, blood and rumen chemistry changes in lambs following supplementation with Se-yeast. Acta Veterinaria Brunensis, 76: 3-8.
7. Fedorak, P. M, and S. E. Hrudey. 1983. A simple apparatus for measuring gas production by methanogeniccultuvesin serum bottles. EnvirinTechnollett, 4: 425-435.
8. Fujihara, T., T. Imamura, and E. A Orden. 2004. Utilization of protozoal selenium in young Goats. Journal of Animal Feed Science, 13: 265-268.
9. Getachew, G., H. P. S. Makkar, and K. Becker. 2002. Tropical browses: content of phenolic compounds, in vitro gas production and stoichiometric relationship between short chain fatty acids and in vitro gas production. Journal of Agricultural Science, 139: 341-352.
10. Hidiroglou, M., D. P. Heaney, and K .J. Jenkins. 1968. Metabolism of inorganic selenium in rumen bacteria. Canadian Journal of Physiological Pharmacology, 46: 229–232.
11. Hidiroglou, M., and K. J. Jenkins. 1974. Influence of defaunation on the utilization of selenomethionine in the sheep. Annales De Biologie Animale, Biochimie, Biophysique, 14: 157–165.
12. Ivan, M., L. Neill, and T. Entz. 2000. Ruminal fermentation and duodenal flow following progressive inoculation of fauna free wethers with major individual species of ciliate protozoa or total fauna. Journal of Animal Science, 78: 750-759.
13. Kim, J., P. J. Vansoest, and G. F. Combs. 1997. Studies on the effects of selenium on rumen microbial fermentation in vitro. Biological Trace Element Research, 56: 203-213.
14. Klasing, K. C., J. P. Goff, and J. L. Greger. 2005. Selenium: Mineral tolerance of animals. The National Academics Press, Washington, DC. pp, 321-347.
15. Markham, R. 1942. A steam distillation apparatus suitable for micro-Kjeldahl analysis. Biochemical Journal, 36(10): 790-795.
16. McDougall, E. I. 1948. The composition and output of sheep in saliva. Biochemical Journal, 43: 99-109.
17. Menke, K. H., L. Rabb, A. Saleweski, H. Steingass, D. Fritz, and W. Schnider. 1979. The estimation of the digestibility and metabolizable energy content of ruminant feed stuffs from the gas production when they are incubated with rumen liquor in vitro. Journal of Agricultural Science, 93: 217-222.
18. Menke, K. H, and H. Steingass. 1988. Estimation of the energetic feed value obtained from chemical analysis and gas production using rumen fluid. Animal Research Development, 28: 7–55.
19. Mihalikova, K., L. Gresakova, K. Boldizarova, S. Faix, L. Leng, and S. Kisidayova. 2005. The effects of organic selenium supplementation on the rumen ciliate population in sheep. Folia Microbiology, 50: 353-356.
20. Naziroglu, M., M. Aksakal, M. Cay, and S. Celik. 1997a. Effects of vitamin E and selenium on some rumen parameters in lamb. Acta Veterinaria Hungarica, 45: 447–456.
21. Naziroglu, M., M. Aksakal, M. Cay, and S. Celik. 1997b. Effects of vitamin E and selenium on rumen protozoa in lambs. Turkish Journal of Veterinary and Animal Sciences, 21: 81–90.
22. Ogimoto, K, and S. Imai. 1981. Atlas of Rumen Microbiology. Japan Scientific Society Press, Tokyo.
23. Opatpatanakit, Y., R. C. Kellaway, I. J. Lean, G. Annison, and A. Kirby. 1994. Microbial fermentation of cereal grains in vitro. Australian Journal of Agricultural Research, 45: 1247-1263.
24. Ramirez, B. J. E., J. L. TortoraHernandez, and M. Huerta. 2001. Main causes of mortalities in dairy goat kids from the Mexican plateau. Small Ruminant Research, 41: 77-80.
25. Shi, L. G., W. J. Xun, W. B. Yue, C. X. Zhang, Y. S. Ren, Q. Liu, Q. Wang, and L. Shi. 2011. Effect of elemental nano-selenium on feed digestibility, rumen fermentation, and purine derivatives in sheep. Animal Feed Science and Technology, 163: 136–142.
26. Underwood, E. J., and N. F. Suttle. 1999. The mineral nutrition of livestock. CAB international, Wallingford, U.K.
27. Van soest, P. J., J. B. Robertson, and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber,and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74: 3583-3592.
28. Wang, C., Q. Liu, W. Z. Yang, Q. Dong, X. M. Yang, D. C. He, P. Zhang, K. H. Dong, and Y. X. Huang. 2009. Effects of selenium yeast on rumen fermentation, lactation performance and feed digestibilities in lactating dairy cows. Livestock Science, 126: 239–44.
29. Xun, W., L. Shi, W. Yue, C. H. Zhang, Y. Ren, Q. Liu. 2012. Effect of high dose nano-selenium and selenium–yeast on feed digestibility, rumen fermentation, and purine derivatives in sheep. Biological Trace Element Research, 150: 130–136.