The Polymorphism of Exon 2 of GOLA-DRB3 Gene in Nadushan Goat Using PCR-RFLP

Document Type : Scientific - Research Articles

Authors

Esmaeil Abbaszadeh ¹

Mohammadreza Mohammad Abadi ¹

Ali Esmailizadeh ¹

Roohollah Alinaghizadeh ²

¹ Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

² Department of Animal Science, Islamic Azad University- Kashmer branch, Kashmer, Iran

10.22067/ijasr.v3i3.11302

Abstract

This study was conducted to determine polymorphism of GOLA-DRB3 in Nadushan goat using PCR-RFLP. Blood samples were randomly taken from 100 goats. DNA was extracted from the blood samples using DIAtom DNA prep Kit. Spectrophotometer and 1% agarose gel were used for determining its quantities and qualities. Exon 2 of DRB 3.2 gene encompassing 285 bp was amplified with heminested-PCR method in two rounds and PCR products were digested with TaqI. Digested PCR products were electrophoresed in 10% Acryl Amide gel or 2% Agarose gel and gels stained with ethidium bromide. Digested PCR products with TaqI included 2 fragments at 122bp and 163bp (T restriction pattern) or undigested fragment at 285bp (t restriction pattern). Population was in Hardy-Weinberg equilibrium (p>0.05). Shanon index, Nei index, observed and expected heterozygosity were 0.68, 0.49, 0.55 and 0.49, respectively. With regard to results of this study, GOLA-DRB3 marker has good efficiency for genetic diversity determining. Using results of this study and former studies and records and quantitative information of locus in this population, we can perform determining and identifying QTL

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