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مصطفی محقق دولت آبادی اسما مرادعلی‌پور فرخنده منصوری فرنگیس فتاحی زینب صالحی

چکیده

نرخ باروری یکی از صفات مهم اقتصادی در گوسفند است، که تحت تأثیر ژنتیک و محیط می­باشد. ژنBMP15  یکی از ‌ژن‌های موثر بر این صفت می‌باشد و جهش­های مختلف در این ژن باعث تغییر راندمان صفات تولیدمثلی و رشد در گوسفند می‌شود. چنانکه باروری بالا در گوسفندان به علت جهش در جایگاه BMP15 با منشأ اووسیتی (ژن فاکتور رشد) شناخته شده است. در این مطالعه از روش PCR-RFLP برای شناسایی چند شکلی‌ جایگاه‌های ژنی FecXI، FecXH وFecXB  استفاده شد. بدین منظور از تعداد 92 رأس میش نژاد لک قشقایی و بهمئی به طور تصادفی خون‌گیری صورت گرفت و استخراج DNA با استفاده از کیت استخراجDNA  انجام شد. قطعات مورد نظر با استفاده از آغازگرهای اختصاصی (قطعه bp 204 از FecXI، قطعه bp 235 از FecXH و قطعه bp 153 از ژن FecXB)، و توسط واکنش زنجیره پلی‌مراز (PCR) تکثیر گردید. پس از مجاورت محصولات PCR با آنزیم­های اختصاصی (XbaI، SpeI وDdeI)، مشاهده گردید که همه قطعات FecXI و FecXH فاقد جایگاه برش برای آنزیم هضم‌کننده بوده و تنها یک الگوی باندی در آن­ها مشاهده شد. این مطالعه تنها وجود آلل­های تیپ وحشی را تأیید کرد. تمامی نمونه‌ها دارای ژنوتیپ هموزیگوت بودند و جهش اینوردل و هانا (جهشی که در حالت هتروزيگوت باعث افزايش ميزان تخمک‌گذاری می‌شود) در آن­ها یافت نشد. براساس نتایج این مطالعه، ژن BMP15 نمی­تواند تأثیری بر میزان چند‌قلوزایی در گوسفندان مورد مطالعه داشته باشد، بنابراین باید به دنبال جهش­ها یا ژن­های دیگری بود.

جزئیات مقاله

کلمات کلیدی

باروری, چندشکلی, ژن BMP15, گوسغند

مراجع
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ارجاع به مقاله
محقق دولت آبادیم., مرادعلی‌پورا., منصوریف., فتاحیف., & صالحیز. (2019). شناسایی جهش‌های FecXI، FecXH و FecXB در ژن BMP15 در گوسفند نژادهای لک قشقایی و بهمئی در استان کهگیلویه و بویر احمد. پژوهشهای علوم دامی ایران, 11(2), 247-257. https://doi.org/10.22067/ijasr.v1397i1.70188
نوع مقاله
علمی پژوهشی- ژنتیک و اصلاح دام و طیور

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