اثر سطوح مختلف روغن کتان بر عملکرد، تجزیه پذیری، برخی از فراسنجه‌های خونی و صفات کمی و کیفی گوشت در بره‌های پرواری

نوع مقاله : علمی پژوهشی - تغذیه نشخوارکنندگان

نویسندگان

• یداله چاشنی دل ¹
• سید مرتضی کاظمی ¹
• اسداله تیموری یانسری ²

¹ دانشگاه علوم کشاورزی و منابع طبیعی ساری

² گروه علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران.

10.22067/ijasr.v11i2.63277

چکیده

  این تحقیق به منظور بررسی تأثیر مصرف سطوح مختلف روغن دانه کتان بر تجزیه پذیری، عملکرد، خصوصیات لاشه و فراسنجه­های خونی در بره های پرواری انجام شد. در آزمایش اول، تجزیه پذیری شکمبه‌ای ماده خشک، پروتئین خام وNDF جیره­های آزمایشی با استفاده از روش کیسه نایلونی و با 3 رأس گوسفند دارای فیستولای شکمبه­ای نژاد زل تغذیه شده در حد نگهداری اندازه­گیری شد. زمان­های شکمبه گذاری در این آزمایش شامل زمان های صفر، 4، 8، 16، 24، 36، 48، 72 و 96 ساعت بود. نتایج نشان داد که تفاوت معنی­داری در  تجزیه پذیری ماده خشک و پروتئین خام بین تیمارها در این آزمایش مشاهده نشد. در آزمایش دوم، 16 رأس بره نر (وزن 2±27 کیلوگرم و سن حدود پنج ماهگی) برای بررسی اثرات سطوح مختلف روغن کتان بر عملکرد، خصوصیات لاشه و فراسنجه­های خونی، در قالب طرح کاملاً تصادفی با 4 جیره آزمایشی (حاوی صفر، 5/1، 3 و 5/4 درصد روغن کتان) به مدت 90 روز پروار شدند. جیره­ها از نظر انرژی و پروتئین قابل متابولیسم مشابه بودند. جیره‌های آزمایشی اثر معنی‌داری بر خوراک مصرفی داشت، علاوه  بر این بره­های تغذیه شده با جیره حاوی 5/4 درصد روغن کتان بیشترین تأثیر منفی را بر قابلیت هضم مواد مغذی جیره کاملاً مخلوط نشان دادند. همچنین جیره حاوی روغن کتان باعث ایجاد اختلاف معنی‌دار در برخی از خصوصیات لاشه بره‌ها نسبت به تیمار شاهد شد. روغن کتان سبب افزایش سهم اسید لینولئیک، اسید لینولنیک و نسبت اسیدهای چرب غیراشباع با چند پیوند دوگانه به اسیدهای چرب اشباع چربی داخل ماهیچه­ای در لاشه بره های پرواری شد. همچنین روغن کتان تأثیر معنی­داری بر فراسنجه­های خونی دام های پرواری داشت. نتیجه کلی حاصل از این تحقیق نشان داد که با افزایش سطح روغن کتان ، میانگین تجزیه پذیری الیاف نامحلول در شوینده خنثی، مصرف خوراک، و قابلیت هضم ظاهری مواد مغذی روند کاهشی داشت ولی در صفات کیفی لاشه بهبود حاصل شد.

کلیدواژه‌ها

• خصوصیات لاشه
• روغن کتان
• فراسنجه های خونی
• لینولنیک اسید

20.1001.1.20083106.1398.11.2.1.3

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عنوان مقاله [English]

Effect of different levels of linseed oil on performance, degradability, digestibility, some blood parameters, quantitative and qualitative traits of meat in fattening lambs

نویسندگان [English]

• yadollah chashnidel ¹
• seyedmorteza kazemi ¹
• Asadollah Teimoury Yansary ²

¹ Sari University of Agricultural Sciences and Natural Resources

² Department of Animal Sciences, Sari University of Agricultural Sciences and Natural Resources

چکیده [English]

Introduction The extracted oil from linen contains about 50% linolenic acid from the family of omega-3 fatty acids. Among edible sources, fish oil and linseed oil are a rich source of omega-3 fatty acids. Linoleic acid and linolenic acid are a key factor in the normal growth and reproduction of animal, were not considered an important issue for animal nutrition. ­Essential fatty acids, including linoleic acid, linolenic acid and arachidonic acid, and in recent years the demand for foods with omega-3 long-chain fatty acid conjugated linoleic acid is high because of important biological role of these fatty acids in the body. Alpha-linolenic acid is an essential fatty acid that cannot be manufactured by the human body, but are essential for the body's metabolism. Although mammals cannot make omega-3 fatty acids, but have limited functionality in the form of fatty acid. Linseed has a high linolenic acid as a substrate for the synthesis of long-chain omega-3 fatty acid that for the (EPA), which is the precursor for eicosanoids. Eicosanoid are similar compounds of hormones that play an essential role in the immune response. In addition, EPA can become (DHA), which is an essential fatty acid in cell tissue layers of the eye and brain. The objective of this study was to evaluate the effects of linseed oil on degradability, performance, carcass characteristics and some blood parameters in fattening lambs
Material and methods In first experiment, ruminal degradability of dry matter, crude protein, NDF and ADF were measured by nylon bag technique using three fistulated Zell sheep that fed at maintenance level. Incubation time consisted of 0, 4, 8, 16, 24, 36, 48, 72 and 96. The experimental animal were kept in individual cage and fed at 8 am and 20 pm with experimental diet to ratio of 70 to 30 forage to concentrate. In the second experiment, the effects of various levels of linseed oil on performance, carcass characteristics and blood parameters were studied. The performed experiment was completely randomized design (CRD) with four diets containing zero, 1.5, 3 and 4.5 percent linseed oil on 16 male lambs with initial average weight 27± 2 kg and 5-6 months old for 90 days. Energy and chemical composition of rations were similar. The experimental diet was balanced using the Small Ruminants Nutrition System (SRNS). Monthly weighing was carried out at the end of the experiment by applying 12 hours of starvation before weighing, and daily feed intake was used to calculate the feed conversion ratio. Data obtained were analyzed by statistical software SAS (version 1.9).
Results and discussion Dry matter degradability significantly affected by experimental treatments (P<0.05). However, the crude protein, NDF and ADF not observed significant difference between treatments. In first experiment, ruminal degradability of dry matter, crude protein, NDF and ADF were measured by nylon bag technique using three fistulated Zell sheep that fed at maintenance level. Incubation time consisted of 0, 4, 8, 16, 24, 36, 48, 72 and 96 h. Dry matter degradability and crude protein not observed significant difference between treatments. In the second experiment, experimental diets had significant effect on feed intake also results of the current experiment showed that linseed oil in 4.5% of dry matter had most negative effects on nutrient digestibility of total mixed ration (P< 0/05). Diet contained linseed oil and thyme oil affected some carcass characteristics significantly compared to other treatments (P<0/05). Linseed oil increased C18:3, C18:2 and P/S ratio (P<0.05). Linseed oil had a significant effect on the blood parameters of treatments.
Conclusion In general, degradation of dry matter results showed that adding linseed oil, had little effect on the degradation of dry matter and protein and numerically, was reduced compared to control. With increasing the level of linseed oil in the diet, the mean of NDF degradability, feed intake, and apparent digestibility of nutrients was a downward trend and did not improve these indices. In this study, adding linseed oil improved daily gain and feed conversion ratio compared to the control. The carcass fatty acids, omega-3 fatty acids increased, which is an indicator of health. The overall result of this study showed that increasing the level of linseed oil decreased the average degradation of NDF, feed intake and digestibility of nutrients but improved the carcass quality traits.

 

کلیدواژه‌ها [English]

• : Linseed oil
• Carcass Characteristics
• Linolenic Acid
• Blood parameters

مراجع

1- Agazzi, A., G. Invernizzi., A. Campagnoli., M. Ferroni., A. Fanelli., D. Cattaneo., A. Galmozzi., M. Crestani., V. Dell’Orto, and G. Savoini. 2010. Effect of different dietary fats on hepatic gene expression in transition dairy goats. Small Ruminant Research, 93: 31-40.

2- Asadi Moghaddam, R. and A. Nikkhah. 1975. The effect of castration on weight gain and carcass traits of lambs eight to twelve months. Journal of College of Agriculture, University of Thran.53-66, Tehran University. Sixth year. No. 4.

3- AOAC International 1990. Official Methods of Analysis of AOAC International (18 Edition, Rev. 2), Association of Official Analytical Chemists International, Gaithersburg, MD, USA.

4- Awawdeh, M., B. Obeidat, A. Abdullah, and W. Hananeh. 2009. Effects of yellow grease or soybean oil on performance, nutrient digestibility and carcass characteristics of finishing Awassi lambs. Animal Feed Science and Technology, 153: 216-227.

5- Bhatt, R. S., S. A. Karim, A. Sahoo, and A. K. Shinde. 2013. Growth Performance of Lambs Fed Diet Supplemented with Rice Bran Oil as Such or as Calcium Soap. Asian-Australia Journal Animal Science, 26: 812–819.

6- Bessa, R. J. B., P. Portugal, I. Mendes, and J. Santos-Silva. 2005. Effect of lipid supplementation on growth performance, carcass and meat quality and fatty acid composition of intramuscular lipids of lambs fed dehydrated lucerne or concentrate. Livestock Production Science, 96: 185-194.

7- Bettero, V. P., J. R. Gandra, H. V. N. Nunes, J. E. Freitas, R. V. Barletta, M. V. Carvalho, E. Detmann, and J. C. Pereira. 2013. Sources of omega-6 fatty acids do not alter the rumen degradation and transit of fibre from dairy cow diets. Journal. Animal. Feed Science, 22: 295–301.

8- Boadi, D., C. Benchaar, J. Chiquette, and D. Masse. 2004. Mitigation strategies to reduce enteric methane emissions from dairy cows: Update review. Journal of Animal Science, 84: 319-335.

9- Boakye, K. and G. S. Mittal. 1993. Changes in pH and water holding properties of Longissimus dorsi muscle during beef ageing. Meat science, 34: 335-349.

10- Chalupa, W., B. Vecchiarelli, D. Sklan, and D. S. Kronfeld. 1985. Response of rumen microorganisms and lactating cows to calcium salts of long chain fatty acids. Journal of Dairy Science, 68: 110. (Abstrac).

11- Childs S., F. Carter, C. Lynch, J. Sreenan, P. Lonergan, A. Hennessy, and D. Kenny. 2008. Embryo yield and quality following dietary supplementation of beef heifers with n-3 poly unsaturated fatty acids (PUFA). Theriogenology, 70: 992- 1003.

12- Chilliard, Y. 1993. Dietary fat and adipose tissue metabolism in ruminants, pigs, and rodents: a review. Journal of Dairy Science, 76: 3897-3931.

13- Chichlowski, M. W., J. W. Schroeder, C. S. Park, W. L. Keller, and D. E. Schimek. 2005. Altering the fatty acids in milk fat by including canola seed in dairy cattle diets. Journal. Dairy Science, 88: 3084-3094.

14- Da Silva, D. C., G. T. D. Santos, A. F. Branco, J. C. Damasceno, R. Kazama, M. Matsushita, J. A. Horst, W. B. R. dos Santos, and H. V. Petit. 2007. Milk production and composition, intake, digestion, blood composition, and fatty acid profile of milk of dairy cows fed whole or ground flaxseed with or without monensin. Journal Dairy Science, 90: 2928_2936.

15- Demirel, G., A. M. Wachira, L. A. Sinclair, R. G. Wilkinson, J. D. Wood, and M. Enser. 2004. Effects of dietary n-3 polyunsaturated fatty acids, breed and dietary vitamin E on the fatty acids of lamb muscle, liver and adipose tissue. British Journal of Nutrition, 91: 551–565.

16- Depeters, E. J., S. J. Taylor, C. M. Finley, and T. R. Famula. 1987. Dietary fat and nitrogen composition of milk from lactating cows. Journal of Dairy Science, 70: 1192-1201.

17- Dong, Y., H. D. Bae, T. A. McAllister, G. W. Mathison, and K. J. Cheng. 1997. Lipid induced depression on methane production and digestibility in the artificial rumen system (Rusitec). Canadian Journal of Animal Science, 77: 269-278.

18- Doreau M. and Y. Chilliard. 1997. Digestion and metabolism of dietary fat in farm animals. British Journal of Nutrition, 78: 15-35.

19- Doreau, M. and Y. Chilliard. 1997. Effects of ruminal or postruminal fish oil supplementation on intake and digestion in dairy cows. British Journal of Nutrition, 37: 113- 124.

20- Douglas, G. N., J. Rehage, A. D. Beaulieu, A. O. Bahaa, and J. K. Drackley. 2007. Prepartum Nutrition Alters Fatty Acid Composition in Plasma, Adipose Tissue, and Liver Lipids of Periparturient Dairy Cows. Journal of Dairy Science, 90: 2959-2941.

21- Emken, E., R. Adlof, H. Rakoff, W. Rohwedder, and R. Gulley. 1992. Human metabolic studies with deuterated alpha-linolenic acid. Nutrition (Burbank, Los Angeles County, Calif), 8: 213-214.

22- Engle, T. E., J. W. Spears, V. Fellner, and J. Odle. 2000. Effects of soybean oil and dietary copper on ruminal and tissue lipid metabolism in finishing steers. Journal of Animal Science, 78: 2721-2713.

23- Folch, J., M. Lees, and G. A. Sloane-Stanley. 1957. Simple method for the isolation and purification of total lipids from animal tissues. Journal of biology Chemical, 26: 497-509.

24- Gonthier, C., A. F. Mustafa, R. Berthiaume, H. V. Petit, R. Martineau, and D. R. Ouellet. 2004. Effects of Feeding Micronized and Extruded Flaxseed on Ruminal Fermentation and Nutrient Utilization by Dairy Cows.Journal of. Dairy Science, 87:1854–1863.

25- Grummer, R. R. 1988.Influence of prilled fat and cacium salt of palm oil fatty acids on ruminal fermentation and nutrient digestibility. Journal of Dairy Science, 71: 117.

26- Harfoot, C. G. 1981. Lipid metabolism in the rumen.Pages 21-55 in lipid metabolism in ruminant animals.1 st ed. W. W. Christie, ed. Pergamon Press, Oxford, United Kingdom.

27- Horton, G. M. J., M. J. Fennell, and B. M. Prasad. 1991. Effect of dietary Garlic (Allium sativum) on performance, carcass composition and blood Chemistry Changes in Broiler Chickens. Candian Journal of Animal Science, 71: 939-942.

28- Huerta-Leidenz N. O., H. R. Cross, D. K. Lunt, L. S. Pelton, J. W. Savell, and S. B. Smith. 1991. Growth, carcass traits, and fatty acid profiles of adipose tissues from steers fed whole cottonseed. Animal. Science, 69: 3665-3672.

29- Ikweegbu, O. A. and J. D. Sutton. 1982. The effect of varing the amount of linseed supplementation on rumen metabolism in sheep. British Journal of Nutrition, 48:365.

30- Jalc, D., A. Potkanski, M. Szumacher-Strabel, A. Cieslak, and M. Certik. 2005. Effect of microbial oil, evening primrose oil and borage oil on rumen fermentation in vitro. Veterinary Medicine-Czech, 50: 480-486.

31- Jenkins, T. C., R. J. Wallace, P. J. Moateand, and E. E. Mosley. 2008. Board-invited review: recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem. Journal Animal Science, 86: 397-412.

32- Jenkins, T. C. and B. F. Jenny. 1992. Nutrient digestion and lactation performance of dairy cows fed combinations of prilled fat and acnola oil. Journal of Dairy Science, 75: 796-803.

33- Jenkins, T. C. and N. Fotouhi. 1990. Effects of Lecithin and Corn oil on site of digestion, ruminal fermentation and microbial protein synthesis in sheep. Journal of Animal Science, 2: 460-466.

34- Karimi, M., M. Ghanjkhanloo, and Gh. Nehzati. 2012. The effect of different sources of fat on rumen digestion and blood factors, in Holstein cows fistulated. Master's thesis, Department of Animal Sciences, College of Agriculture, Tehran University (In Persian).

35- Kennelly, J. J. 1996. The fatty acid composition of milk fat as influenced by feeding oilseeds. Animal Feed Science and Technology, 60: 137-152.

36- Kronberg, S. L., E. J. Scholljegerdes, A. N. Lepper, and E. P. Berg. 2011. The effect of flaxseed supplementation on growth, carcass characteristics, fatty acid profile, retail shelf life and sensory characteristics of beef from steers finished on grasslands of the northern Great Plains. Journal of Animal Science, 89: 2892-2903.

37- Lough, D. S., M. B. Solomon, T. S. Rumsey, T. H. Elsasser, L. L. Slyter, S. Kahl, and G. P. Lynch. 1992. Effects of dietary canola seed and soy lecithin in high-forage diets on cholesterol content and fatty acid composition of carcass tissues of growing ram lambs. Journal of Animal Science, 70: 1153-1158.

38- Machmullar, A., D. A. Ossowki, A. Wanner, and M. Kreuzer. 1998. Potential of various fatty feeds to reduce methane release from rumen fermentation in vitro. Animal Feed Science and Technology, 71: 117-130.

39- Maddock, T. D., M. L. Bauer, K. B. Koch, V. L. Anderson, R. J. Maddock, G. Barcelo-Coblijn, E. J. Murphy, and G. P. Lardy. 2006. Effect of processing flaxseed in beef 17 feedlot diets on performance, carcass characteristics, and trained sensory panel ratings. Journal of Animal Science, 84:1544-1551.

40- Menke K. H. and H. Steingass. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research Development, 28: 7-55.

41- Metcalfe, L. and A. Schmitz. 1961. The rapid preparation of fatty acid esters for gas chromatographic analysis. Analytical Chemistry, 33: 363-364.

42- Miller, G. J. and R. W. Rice. 1967. Lipid metabolism in lambs as affected by fattening rations of roughage and concentrate. Journal. Animal. Science, 26: 1153-1159.

43- Maia, M. R. G., L. C. Chaudhary, C. S. Bestwick, A. J. Richardson, N. McKain, T. R. Larson, I. A. Graham, and R .J. Wallace. 2010. Toxicity of unsaturated fatty acids to the biohydrogenating ruminal bacterium, Butyrivibrio fibrisolvens. BMC Microbiology, 10: 52-62.

44- Mostofi, S. 2000. The Study of oilseeds market and its products, planning and research project of the Institute of Agricultural Economics. Tehran: Ministry of Agriculture. (In Persian).

45- Najafi, H., S. Zeinaldini, and M. Ghanjkhanloo. 2013. The effect of fat source on performance, carcass quality and composition of fatty acids into the muscle of the male goat Mahabad. Tehran University master's thesis. (In Persian).

46- Najafi, M., S. Zeinoaldini, M. Ganjkhanlou, H. Mohammadi, D. Hopkins, and E. Ponnampalam. 2012. Performance, carcass traits, muscle fatty acid composition and meat sensory properties of male Mahabadi goat kids fed palm oil, soybean oil or fish oil. Meat science, 92: 848-854.

47- Nelson, G. J. and R. G. Ackman 1988. Absorption and transport of fat in mammals with emphasis on n-3polyunsaturated fatty acids. Lipids, 23: 1005-1014.

48- Nigdi, M. E., S. C. Loerch, F. L. Fluharty, and D. L. Palmiquist. 1990. Effect of calcium soaps of long chain fatty acids on feedlot performance, carcass characteristics and ruminal metabolism of steers. Journal Animal Science, 68: 2555–2565.

49- Nikokyris, P.N., K. Kandylis, and K. Deligiannis. 1999. Effects of varying levels of dietary free gossypol in whole cottonseed on physiological responses of growing-fattening lambs. Science Food Agriculture,79: 1969-1981.

50- NRC. 2007. Nutrient Requirments of Small ruminant. 7th edition. Nattiona. Academy Press, Washington, DC.

51- Ørskov, E. R. and I. M. McDonald. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science, 92: 499-503.

52- Oba, M., G. Thangavelu, M. Dehghan-banadaky, and D. J. Ambrose. 2009. Unprocessed whole flaxseed is as effective as dry-rolled at increasing (-linolenic acid concentration in milk of dairy cows. Livestock. Science, 122: 73-76.

53- Palmquist, D. L., A. L. Lock, K. J. Shingfield, and D. E. Bauman. 2005. Biosynthesis of conjugated linoleic acid in ruminants and humans. Pages 179–217 in Advances in Food and Nutrition Research. Vol. 50. S.-L. Taylor, ed. Elsevier Academic Press, San Diego, CA.

54- Peng, Y. S., M. A. Brown, J. P. Wuand, and Z. Liu. 2010. Different oilseed supplements alter fatty acid composition of different adipose tissues of adult ewes. Meat Science, 85: 542–549.

55- Petit, H. V. 2003. Digestion, Milk Production, Milk Composition, and Blood Composition of Dairy Cows Fed Whole Flaxseed1. Journal of Dairy Science, 85: 1482–1490.

56- Petit, H. V. 2010. Feed intake, milk production and milk composition of dairy cows fed flaxseed Review, Canad. Journal. Animal. Science, 2: 181.5.161.

57- Ratnayake, W., R. G. Ackman, and H. W. Hulan. 1989. Effect of redfish meal enriched diets on the taste and n 3pufa of 42 day old broiler chickens. Journal of the Science of Food and Agriculture, 49: 59-74.

58- Santos-Silva, J., I. Mendes, P. Portugal., and R. Bessa. 2004. Effect of particle size and soybean oil supplementation on growth performance, carcass and meat quality and fatty acid composition of intramuscular lipids of lambs. Livestock Production Science, 90: 79-88.

59- SAS. 2001. Statistical Analysis System User’s Guide: Statistics. SAS Institute, Cary, NC.

60- Schauff, D. J. and J. H. Clark. 1992. Effect of feeding diets containing calcium salts of fatty acids of long-chain fatty acids to lactating dairy cattle. Journal of Dairy Science, 2990-3002.

61- Scollan, N. D., N. J. Choi, E. Kurt, A. V. Fisher, M. Enser, and J. D. Wood. 2001. Manipulating the fatty acid composition of muscle and adipose tissue in beef cattle. British Journal of Nutrition, 85: 115-124.

62- Sinclair, A. L., S. L. Cooper, J. A. Huntington, R. G. Wilkinson, K. G. Hallet, M. Enser, and J. Wood. 2005. In vitro biohydrogenation of n-3 polyunsaturated fatty acids protected against ruminal microbial metabolism. Animal Feed Science and Technology, 123-124: 579-596.

63- Van Soest, P. J., J. B. Robertson, B. A. and Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharide in relation to animal soybean meal on nitrogen utilization by ruminants. Journal of Animal Science, 63: 879-886.

64- Vatansever, L., E. Kurt, M. Enser, G. R. Nutem, N. D. Scollan, J. D. Wood, and R. I. Richardson. 2000. Shelf life and eating quality of beef from cattle of different breeds given diets differing in n−3 polyunsaturated fatty acid composition. Animal. Science, 71: 471–482.

65- Wachira, A. M., L. A. Sinclair, R. G. Wilkinson, M. Enser, J. D. Wood, and A. V. Fisher. 2002. Effects of dietary fat source and breed on the carcass composition, n-3 polyunsaturated fatty acid and conjugated linoleic acid content of sheep meat and adipose tissue. British Journal of Nutrition, 88: 697-709.

66- Woods, V. B. and A. M. Fearon. 2009. Dietary sources of unsaturated fatty acids for animals and their transfer into meat, milk and eggs: A review. Livestock Science, 126: 1-20.

67- Zinn, R. A. 1989. Influence of level and source of dietary fat on its comparative feeding value in finishing diets for steers: Metabolism. Journal of Animal Science, 67: 1038-1049.

68- Zinn, R. A. and Y. Shen.1996. Interaction of dietary calcium and supplemental fat on digestive function and growth performance in feedlot steers. Journal of Animal Science, 74: 2303-2309.

69- Zinn, R. A., S. K. Gulati, A. Plascencia, and J. Salinas. 2000. Influence of ruminal bio hydrogenation on the feeding value of fat in finishing diets for feedlot cattle. Journal Animal Science. 78: 1738–1746.