Assessing Polymorphism in BM6444, INRA13 and Oarhh35 Microsatellite Markers Associated with Inhibin Gene in Sanjabi Sheep

Document Type : Scientific - Research Articles

Authors

• bejan Solimani ¹
• burumand Chaharaein ²
• Ghodratollah Rahimimianji ³

¹ Ferdowsi University of Mashhad

² Ferdwosi University of Mashhad

³ Department of Animal Sciences, Faculty of Animal Sciences and Fisheries, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran.

10.22067/ijasr.v4i1.13916

Abstract

The purpose of this study was to identify the rate of polymorphism in BM6444, INRA13 and orahh35
microsatellite markers associated with Inhibin gene in Sanjabi sheep. For this purpose, blood samples were taken
randomly from 100 Sanjabi sheep (78 females and 22 males) at Mehregan Breeding Station at Kermanshah.
Modified salting out method was employed for extracting DNA. Subsequently, Polymerase Chain Reaction
(PCR) was carried out with specific primers pairs for amplification of microsatellite marker sites. The PCR
products were electrophoreses on acryl-amid gel. For detecting genotypes within the population, silver staining
method was used. Genetic analyses on genotypic data were carried out using POPGEN 1.32 and Cervus 2.0
softwares. Results showed, three alleles (C: 0.42, B: 0.28, A: 0.29), and 2 genotypes respectively, (AB: 0.32,
BC: 0.68) for oarhh35 marker. For this marker, PIC index was 0.71, and the effective number of allele was 2.48.
INRA13 had six alleles (A: 0.21, B: 0.25, C: 0.21, D: 0.09, E: 0.11 and F: 0.12) and three genotypes (AB: 0.42,
BD: 0.29 and CD: 0.27) with a PIC index of 0.72 and an effective number of allele as 5.21. For BM6444
marker, four alleles (A: 0.18, B: 0.38, C: 0.31 and D: 0.12) and four genotypes (AD: 0.32, CD: 0.18, AB: 0.12
and BC: 0.38) were identified. For this marker, the PIC index and the effective number of allele were 0.62 and
3.42, respectively

Keywords

• Sanjabi Sheep
• Microsatellite Markers
• Inhibin
• oarhh35
• BM6444
• INRA135

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