تعیین ترکیب اسیدهای چرب سیس و ترانس گوشت و دنبه گوسفندان بلوچی در استان خراسان جنوبی

نوع مقاله : علمی پژوهشی - تغذیه نشخوارکنندگان

نویسندگان

1 گروه علوم دامی، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران.

2 علوم پزشکی بیرجند

3 گروه شیمی، دانشگاه بیرجند، بیرجند، ایران.

چکیده

به منظور تعیین ترکیب اسیدهای چرب دنبه و گوشت گوسفندان بلوچی استان خراسان جنوبی، تعداد 5 نمونه گوشت ران و راسته و 5 نمونه دنبه از 5 رأس گوسفند از محل کشتارگاه های دام، هشت منطقه استان خراسان جنوبی یعنی شهرستان های بیرجند، سربیشه، نهبندان، درمیان، قائن، فردوس، سرایان و بشرویه (از 40 رأس گوسفند در سطح استان) تهیه و جمع آوری گردید و نمونه ها تا زمان استخراج چربی آن ها در فریزر oC80- نگهداری شد. پس از جمع آوری تمام نمونه ها، چربی استخراج و متیله گردید سپس نوع و درصد اسیدچرب در مقایسه با زمان پیک و مقدار پیک استاندارد و روش استاندارد داخلی تعیین گردید و سپس آنالیز آماری داده ها به کمک نرم افزار SAS انجام شد. تجزیه و تحلیل داده ها نشان داد درصد مجموع اسیدهای چرب اشباع دنبه گوسفندان از گوشت بالاتر بود (05/0>P). مجموع درصد اسیدهای چرب غیراشباع با یک پیوند دوگانه و غیراشباع با چند پیوند دوگانه در گوشت بالاتر از چربی بود (05/0>P). درصد اسیدهای چرب امگا-3 در گوشت بالاتر از دنبه ولی اختلاف آن‌ها معنی‌دار نبود. درصد اسیدهای چرب امگا-6 و اسیدهای چرب ترانس در دنبه بطور معنی‌داری بالاتر از گوشت گوسفندان بود (05/0>P). نسبت اسیدهای چرب غیراشباع با چند پیوند دوگانه به اسیدهای چرب اشباع (PUFA/SFA) در گوشت بالاتر از دنبه گوسفندان بود. در مجموع تحلیل داده‌ها نشان می‌دهد که هر چند درصد مجموع اسیدهای چرب ترانس دنبه گوسفندان بالاتر بود ولی از شاخص استاندارد ایران پائین‌تر می‌باشد. تحلیل ترکیب اسیدهای چرب گوشت و دنبه گوسفندان مناطق مختلف استان خراسان جنوبی بدلیل تفاوت در ترکیب چراگاه مورد استفاده با هم اختلاف معنی‌داری نشان داد. از طرف دیگر با توجه به نسبت بالای اسیدهای چرب غیراشباع و پائین تر بودن درصد اسیدهای چرب اشباع گوشت در مقایسه با دنبه، کیفیت گوشت از دنبه گوسفندان استان خراسان جنوبی بهتر می‌باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation the cis and trans Fatty Acid Composition in the Sheep's Fat-Tail and Meat of Baluchi Sheep in South Khorasan Province

نویسندگان [English]

  • seyyed javad Hosseini-vashan 1
  • Mohammad Malekaneh 2
  • Ali Allahressani 3
1 Department of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran.
2 Birjand University of Medical Sciences
3 Department of Chemistry, Birjand University, Birjand, Iran.
چکیده [English]

Introduction The common isoform of fatty acids in animal and herbal lipids are cis fatty acids, however in some sources such as milk and meat of ruminant, the trans fatty acids are more abundant as compared to the plant lipids. The trans fatty acids are very important because they increase the cardiovascular and heart attack. The heart disorders were increased by enhancing the concentration of omega-6 fatty acids and decreasing the concentration of omega-3 fatty acids in ration and blood. The types of pasture cause alter the fatty acid composition of lamb meat. For evaluation of heart function and health of heart, the artherosclosis and thrombogensis were calculated by the concentration of saturated and unsaturated fatty acids. Therefore, the subject of this study, evaluation of the fatty acid profiles of sheep's fat-tail and meat at different places of South Khorasan Province.
Material and Methods Five samples of meat and fat-tail from the animal's abattoir in Birjand, Sarbisheh, Asadieh, Ferdows, Saraian, Ghaen, Boshroieh and Nehbandan cities were collected and frozen (40 samples of meat and fat-tail). The samples were frozen in -80oc freezer. Then, the oils were separated and the fatty acids were extracted and methylated by the method of Morrison and Smith (1964). After that, the 0.5 µl of methylated fatty acids were injected to gas chromatography instruments (Varian 4200). The capillary column CPSill 88 was used. The type of each fatty acid was designated with compare to the retention time in the standard peak. The quantity of fatty acids was determined by internal standard method. To evaluation the fatty acids ratio for health, the artherosclosis and thrombogensis indices were calculated based on saturated and unsaturated fatty acids. The data were analyzed by SAS software, and the mean were compared by tukey test (α=0.05).
Results and Discussion The analysis of data revealed that the percentage of saturated fatty acids (SFA) was higher at fat-tail as compared to meat of sheeps. The meat had higher MUFA and PUFA (P

کلیدواژه‌ها [English]

  • Cis and Trans fatty acids
  • Fat-tail
  • Meat sheep
  • South Khorasan Province

مراجع

1- Aro, A., A. Kardinnal., I. Salminen., J. Kark., and R. A. Riemersma. 1995. Delgado-Rodriguez M, et al. Adipose tissue isomeric trans fatty acids, and risk of myocardial infarction in nine countries: the EURAMIC study. Lancet, 345: 273-78.
2- Aro, A. J., M. Antoine., L. Pizzoferrato., O.Reykdal., and G. Van Poppel. 1998. Trans fatty acids in dairy and meat products from 14 European Countries: The transfair Study. Journal of Food Composition Analysis, 11: 150–60.
3- Ascherio, A., C. H. Hennekens., J. Buring., C. Master., M. Stampfer., and W. C. Willett. 1994. Trans fatty acids intake and risk of myocardial infarction. Circulation, 89: 94-101.
4- Beaulieu, A. D., J. Drackley., and N. R. Merchen. 2002. Concentrations of conjugated linoleic acid (cis-9., trans-11-octadecadienoic acid) are not increased in tissue lipids of cattle fed a high-concentrate diet supplemented with soybean oil. Journal of Animal Science, 80: 847–861.
5- Brown, S. A., J. Morrisett., E. Boerwinkle., R. Hutchinson., and W. Patsch. 1993. The relation of lipoprotein[a] concentrations and apolipoprotein[a] phenotypes with asymptomatic atherosclerosis in subjects of the Atherosclerosis Risk in Communities (ARIC) study. Arteriosclerosis Thrombosis, 13: 1558-1566.
6- Dolecek, T. A. 1992. Epidemiological evidence of relationships between dietary polyunsaturated fatty acids and mortality in the multiple risk factor intervention trial. Proceedings of the Society for Experimental Biology and Medicine, 200: 177–182.
7- Doreau, M., and A. Ferlay. 1994. Digestion and utilization of fatty acids by ruminants. Animal Feed Science and Technology, 45: 379–396.
8- Dreiucker, J., and W. Vetter. 2011. Fatty acids patterns in camel, moose, cow, and human milk as determined with GC/MS after silver ion solid phase extraction. Food Chemistry, 126: 762-771.
9- Enser, M., K. Hallett., B. Hewitt., G. A. J. Fursey., and J. D. Wood. 1996. Fatty acid content and composition of English beef, lamb and pork at retail. Meat Science, 42: 443–456.
10- Gebauer, S. K., J. M. Chardigny., M. U. Jakobsen., B. Lamarche., A. L. Lock., S. D. Proctor., and D. J. Baer. 2011. Effects of ruminant trans fatty acids on cardiovascular disease and cancer: a comprehensive review of epidemiological, clinical, and mechanistic studies. Advances in Nutrition, 2(4): 332-54.
11- Garaffo, M. A., R. Vassallo-Agius., Y. Nengas., E. Lembo., R. Rando., R. Maisano., G. Dugo., D. Giuffrida. 2011. Fatty acids profile, atherogenic (ia) and thrombogenic (it) health lipid indices, of raw roe of blue fin tuna (thunnus thynnus l.) and their salted product “Bottarga”. Food and Nutrition Sciences, 2: 736-743.
12- Gharemanpour, F., M. Firuzray., M. Darabi Amin., and A. Mohebi. 2006. Adipose tissue trans fatty acids and risk of coronary artery disease. Razi Journal of Medicine Science, 13(50): 135-145. [In Persian].
13- Hosseini-Vashan, S. J., N. Afzali., M. Malekaneh., M. A. Nasseri., and A. Ressani. 2009. Effect of different levels of linseed and safflower seed on modifying yolk fatty acids content and antibody titter of laying hens. Iranian Journal of Animal Science Researchers, 1(2): 87-98. [In Persian].
14- Hu, F. B., and W. C. Willet. 2001b. Diet and coronary heart disease: Findings from the Nurses’ Health Study and Health Professionals’ Follow-up Study. Journal of Nutrition Health Aging, 5: 132–138.
15- Idris C. A., and K. Sundram. 2002. Effect of dietary cholesterol, trans and saturated fatty acids on serum lipoproteins in non-human primates. Asia Pacific Journal Clinical Nutrition, 11:S408-15.
16- Katan, M. B., P. L. Zock., and R. P. Mensink. 1995. Ronald trans fatty acids and their effects on lipoproteins in humans. The Annual Review of Nutrition, 15: 472-93.
17- Komprda, T., J. Kuchtik., A. Jarošova., E. Dračkova., L. Zemanek., and B. Filipčik. 2012. Meat quality characteristics of lambs of three organically raised breeds. Meat Science, 91: 499-505.
18- Kris-Etherton, P., and S. Yu. 1997. Individual fatty acids on plasma lipids and lipoproteins: human studies. American Journal of Clinical Nutrition, 65: 1628S–1644S.
19- Lanz., M., C. Fabro., M. Scerra., M. Bella., D. M. R. Brogna., and P. Pennisi. 2011. Lamb meat quality and intramuscular fatty acid composition as affected by concentrates including different legume seeds. Italian Journal of Animal Science, 10: 87-94.
20- Masanori, S. 2002. Trans Fatty Acids: Properties, Benefits and Risks. Journal Health Science. 48(1): 7–13.
21- McGee, D. L., D. M. Reed., K. Yano., A. Kagan., and J. Tillotson. 1984. Ten-year incidence of coronary heart disease in the Honolulu Heart Program: Relationship to nutrient intake. American Journal of Epidemiology, 119: 667–76.
22- Morrison, W. R., and L. M. Smith. 1964. Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride methanol. Journal Lipid Research, 5: 600-608.
23- Mozaffarian, D., E. B. Rimm., I. B. King., R. L. Lawler., G. B. McDonald., and W. C. Levy. 2004. Trans fatty acids and systemic inflammation in heart failure. American Journal of Clinical Nutrition, 80: 1521-5.
24- Mozaffarian, M. D., and H. Y. Jason. 2011. Omega-3 fatty acids and cardiovascular disease effects on risk factors, molecular pathways, and clinical events. Journal of American of College of Cardiovascular, 58: 2047-67.
25- Mozaffarian, D., A. Aro., and W. C. Willett. 2009. Health effects of trans-fatty acids: experimental and observational evidence. European Journal of Clinical Nutrition, 63: S5–21.
26- Orellana, C., F. Peña., A. Garcia., J. Perea., J. Martos., V. Domenech., and R. Acero. 2009. Carcass characteristics, fatty acid composition, and meat quality of Criollo Argentino and Braford steers raised on forage in a semi-tropical region of Argentina. Meat Science, 81: 57-64.
27- Palmquist, D. L., N. St-Pierre., and K. E. McClure. 2004. Tissue fatty acid profiles can be used to quantify endogenous rumenic acid synthesis in lambs. Journal of Nutrition, 134: 2407–2414.
28- Pfalzgraf, A., M. Timm., and H. Steinhart. 1994. Content of trans-fatty acids in food. Ernahrungswiss, 33: 24-43.
29- Ramirez-Retamal, J., R. M. Morales., E. Martinez., and R. D. Barra. 2014. Effect of the type of pasture on the meat characteristics of chilote lambs. Food and Nutrition Sciences, 5: 635-644.
30- Santora, J., D. L. Palmquist., and K. L. Roehrig. 2000. Trans vaccenic acid is desaturated to conjugated linoleic acid in mice. Journal of Nutrition, 130: 208–215.
31- SAS Institute. SAS Stat User's Guide. Version 9.1 ed. SAS Inst. Inc., Cary, NC. 2008.
32- Scerra, M., P. Caparra., F. Foti., C. Cilione., G. Zappia., C. Motta., and V. Scerra. 2011. Intramuscular fatty acid composition of lambs fed diets containing alternative protein sources. Meat Science, 87: 229-233.
33- Senso, L. M., D. Suarez., T. Ruiz-Cara., and M. Garcia-Gallego. 2007. On the possible effects of harvesting season and chilled storage on the fatty acid profile of the fillet of farmed gilthead Sea Bream (Sparus aurata). Food Chemistry, 101: 298-307.
34- Teye, G. A., P. R. Sheard., F. M. Whittington., G. R. Nute., A. Stewart., and J. D. Wood. 2006a. Influence of dietary oils and protein level on pork quality. 1. Effects on muscle fatty acid composition, carcass, meat and eating quality. Meat Science, 73: 157–165.
35- Teye, G. A., J. D. Wood., F. M. Whittington., A. Stewart., and P. R. Sheard. 2006b. Influence of dietary oils and protein level on pork quality.2. Effects on properties of fat and processing characteristics of bacon and frankfurter-style sausages. Meat Science, 73: 166–177.
36- Ulbritch, T. L.V., and T. D. A. Southgate. 1991. Coronary Heart Disease: Seven Dietary Factors. Lancet, 338: 985-992.
37- Valsta, L. M., H. Tapanainen., and S. Mannisto. 2005. Meat fats in nutrition – a review. Meat Science, 70:525-530.
38- Zock, P. L., and R. P. Mensink. 1996. Dietary trans-fatty acids and serum lipoproteins in humans. Current Opinion Lipid, 7:34-37.
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  • تاریخ دریافت: 24 مهر 1393
  • تاریخ پذیرش: 24 مهر 1393
  • تاریخ اولین انتشار: 01 فروردین 1395

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