اثر آنتی‌اکسیدانی ملاتونین بر فراسنجه‌های کیفی منی قوچ عربی بعد از فرآیند انجماد- یخ‌گشایی

محمدی, صدیقه; ممویی, مرتضی; طباطبایی وکیلی, صالح; فیاضی, جمال

doi:10.22067/ijasr.v10i2.61116

اثر آنتی‌اکسیدانی ملاتونین بر فراسنجه‌های کیفی منی قوچ عربی بعد از فرآیند انجماد- یخ‌گشایی

نوع مقاله : علمی پژوهشی- فیزیولوژی

نویسندگان

¹ دانشگاه کشاورزی و منابع طبیعی رامین خوزستان

² کشاورزی و منابع طبیعی رامین خوزستان

³ دانشـکده علـوم دامی، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان، ملاثانی، اهواز، ایران

10.22067/ijasr.v10i2.61116

چکیده

هدف از این پژوهش، بررسی اثر افزودن سطوح متفاوت ملاتونین (صفر، 5/0، 1، 2 و 4 میلی‌مول بر میلی‌لیتر) به رقیق‌کننده بر فراسنجه‌های کیفی منی قوچ عربی پس از فرآیند انجماد-یخ گشایی بود. این تحقیق در پاییز 1394 در ایستگاه دامپروری دانشگاه کشاورزی و منابع طبیعی رامین خوزستان انجام گرفت. نمونه‌های منی توسط دستگاه الکترواجاکولیتور از 6 رأس قوچ با متوسط وزن 3 ±73 کیلوگرم، هفته‌ای دو بار جمع‌آوری شد. نمونه‌های اسپرم بعد از انجماد و یخ‌گشایی از نظر تحرک با کمک سامانه آنالیز رایانه‌ای اسپرم (CASA) اندازه‌گیری شد. pH٬ درصد اسپرم‌های زنده و ناهنجار، سلامت غشا و ظرفیت آنتی‌اکسیدانی تام نیز بعد از فرآیند یخ‌گشایی اندازه‌گیری شد. نتایج حاصل از این آزمایش نشان داد که رقیق‌کننده‌ی حاوی سطح 5/0 میلی‌مول ملاتونین در مقایسه با گروه شاهد موجب بهبود میزان تحرک‌، زنده‌مانی و سلامت غشای پلاسمایی اسپرم قوچ عربی شد. بیشترین مقدار ظرفیت آنتی‌اکسیدانی تام پلاسمای منی در سطح 1 میلی‌مولار ملاتونین مشاهده شد‌. اثر ملاتونین بر روی pHمنی در همه سطوح اختلاف معنی‌داری نسبت به گروه شاهد ایجاد نکرد. به‌طور کلی افزودن سطح 5/0 میلی‌مول بر میلی‌لیتر ملاتونین به رقیق‌کننده تریس موجب بهبود اغلب فراسنجه‌های کیفی اسپرم و ظرفیت آنتی‌اکسیدانی تام پلاسمای منی پس از فرآیند انجماد- یخ‌گشایی در قوچ عربی شد.

کلیدواژه‌ها

20.1001.1.20083106.1397.10.2.1.6

عنوان مقاله [English]

Antioxidant Effect of Melatonin on Arabic Ram Semen Parameters after Freeze - Thawing Process

نویسندگان [English]

sedighe mohamadi ¹
morteza mamouei ²
Saleh Tabatabaei Vakili ³
jamal fayazi ¹

¹ Ramin Agriculture and Natural Resources University of Khuzestan

² Agriculture and Natural Resources University of Ramin of Khuzesta

³ Faculty of Animal Sciences, Ramin Khuzestan University of Agriculture and Natural Resources, Malasani, Ahvaz, Iran

چکیده [English]

Introduction Semen collection evaluation and addition of preservatives to increase storage period of sperm are essential for successful artificial insemination. Cryopreservation of spermatozoa is becoming more important because of new clinical requirements and current clinical practice. Although frozen-thawed semen has great practical beneﬁts for reproduction, it is widely reported that the cryopreservation process involving cooling, freezing, and thawing induces serious detrimental changes in sperm functions. The viability, motility and membrane integrity of mammalian spermatozoa decrease during the cryopreservation process. Many studies exist as regards the effects of antioxidants on the cryopreservation aimed at improving the quality of post-thaw semen. Antioxidant molecules could decrease the impact of oxidative stress and therefore improve Sperm quality following the freeze–thawing process. Melatonin (N-acetyl-5-methoxytryptamine), a derivative of tryptophan, is mainly synthesized and secreted by the pineal gland during the night in reaction to changes in light levels. Melatonin can stimulate the activity of antioxidant enzymes such as SOD and GSH-Px. melatonin scavenges a variety of reactive oxygen and nitrogen species in vivo and in vitro, signifying it has a powerful non-enzymatic antioxidant property. It has been shown that the spermatozoa undergo a freeze–thawing process produced high concentrations of reactive oxygen species. The aim of this study was to investigate the effects different levels of melatonin supplementation (0, 0.5, 1, 2 and 4 mM/ml) in extender on semen characteristics the Arabic ram after freezing-thawing.
Materials and Methods This research was performed at Ramin Agriculture and Natural Resources University of Khuzestan in the fall in 2015. Semen samples were collected from 6 Arabic ram with an average weight 73 ± 3 kg by electro ejaculator twice a week. Sperm samples motility were assessed by Computer Aided Sperm Analysis (CASA) after freezing and thawing. The pH, viability, abnormal sperm percentage, membrane integrity and total antioxidant capacity were also assessed after freezing-thawing process. Pearson correlation test was used to assess correlation of melatonin with routine sperm parameters (pH, viability, abnormal sperm percentage, membrane integrity and total antioxidant capacity of plasma). Data analysis was performed using SAS software. P

کلیدواژه‌ها [English]

Sperm
Motility
Membrane Integrity
Survival
Total Antioxidant Capacity

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