تأثیر کنجاله کنجد بر الگوی اسیدهای چرب گوشت و قابلیت هضم در بره‌های نژاد بلوچی

نوع مقاله : علمی پژوهشی - تغذیه نشخوارکنندگان

نویسندگان

1 پردیس کشاورزی و منابع طبیعی، دانشگاه بیرجند، بیرجند، ایران

2 گروه تغذیه دام و طیور، دانشکده کشاورزی و منابع طبیعی دانشگاه بیرجند، بیرجند، ایران

3 گروه علوم دامی، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران

چکیده

هدف از این پژوهش، بررسی جایگزینی کنجاله کنجد با کنجاله سویا به عنوان یک فراورده فرعی فراوان و در دسترس و همچنین غنی از ترکیبات مفید نظیر اسیدهای چرب ضروری و آنتی­اکسیدان­ها در تغذیه بره­های پرواری بود.در این آزمایش 21 رأس بره نر نژاد بلوچی با میانگین وزنی3±30 کیلوگرم به طور تصادفی در 3 گروه قرار داده شد و هر گروه با یکی از سه جیره آزمایشی که حاوی سطوح مختلف کنجاله کنجد بود تغذیه شدند. جیره‌های آزمایشی عبارت بودند از: 1- جیره شاهد، جیره فاقد کنجاله کنجد (حاوی 12 درصد سویا) 2- جیره دوم حاوی 6 درصد کنجاله کنجد که جایگزین نصف سویا شد (حاوی 6 درصد کنجاله کنجد+6 درصد کنجاله سویا) 3- جیره سوم حاوی 12 درصد کنجاله کنجد بود که به طور کامل جایگزین کنجاله سویا شد. دوره آزمایش 75 روز بود و بره­ها در پایان دوره آزمایش کشتار شدند. نتایج نشان داد کنجاله کنجد تاثیری بر مصرف خوراک، افزایش وزن و قابلیت هضم مواد مغذی نداشت. نتایج مربوط به الگوی اسیدهای چرب عضله کاهش اسیدهای چرب C:16 و C:18 را در جیره­های محتوی کنجاله کنجد نشان داد، همچنین واکسینیک اسید C18:1 (VA) trans-11 و رومینیک اسید CLA cis-9 trans-11C18:2(RA)با افزایش سطح کنجاله کنجد به طور معنی­داری افزایش یافت. کنجاله کنجد باعث افزایش MUFA، PUFA،MUFA:SFA  و PUFA:SFA و کاهش SFA و AI شد. نتایج حاصل از این پژوهش نشان داد جایگزین کردن کنجاله کنجد با سویا بدون تاثیر منفی بر عملکرد تا سطح 12 درصد، منجر به بهبود ارزش تغذیه­ای گوشت شد.

کلیدواژه‌ها


عنوان مقاله [English]

Influence of Sesame Meal on Meat Fatty Acid Profile, and Digestibility in Fattening Lambs of Baluchi Breed

نویسندگان [English]

  • soheyla shabkhan shokatabad 1
  • Moslem Bashtani 2
  • Seyyed Homayoun Farhangfar 3
1 Department, Faculty of Agriculture, University of Birjand, Birjand, Iran
2 Animal and Poultry Nutrition Department, Faculty of Agricultural and Natural Resources, University of Birjand, Birjand, Iran.
3 Department of Animal Science, Birjand Faculty of Agriculture, Birjand, Iran
چکیده [English]

Introduction Sesame with the scientific name ‘Sesamum Indicum’ belongs to the Pedaliaceae family. Sesame seeds have high oil content (42-56%) and crude protein (20-25%), as well as a source of minerals, especially calcium, phosphorus, potassium and iron. The main fatty acids in sesame include: linoleic acid (40.4 to 47.9%), oleic acid (35.9 to 42.3%), palmitic acid (7.9% to 12%) and stearic acid (6.1 to 4.8 %). Historically, the purpose of agricultural research has been focused on increasing production efficiency so that less emphasis has been on improving the profile of food products. Therefore, scientists and producers are interested in research and agricultural activities that can improve the nutritional profile of food products. Changes in animal nutrition can significantly increase the concentration of bioactive components (such as conjugated linoleic acid and omega-3 fatty acids) in animal products. The most effective strategy is to supplement ruminants with different oils or oils rich in linoleic acid or linolenic acid.
Materials and Methods In this study, 21 lambs with average initial weight of 30 ±3 kg were used. The experiment was conducted in a completely randomized design with three treatments including 0, 6 and 12% replacement of sesame meal with soybean meal with 7 replicates for 75 days (14 days adaptation). Experimental diets were adjusted using the SRNS transcription software (NRC 2007). Feed was given daily at 8 am and 4 pm. After slaughter of animals, samples of Longissimus dorsi muscle (ribs 12 and 13) were removed from the left carcass and after packaging to measure fatty acids in Freezer-20 C was maintained. The fatty acid composition of sesame meal and muscle were measured. The internal marker was used to determine apparent digestibility of nutrients.
Results and Discussion Replacing sesame meal (SM) with soybean meal had no effect on nutrient digestibility and performance (p> 0.05). The effects of added dietary fat on performance of ruminants are reported to be varied. Such variability could be associated with differences between experiments in terms of composition of the basal diet (i.e., energy density and level of grain), level of fat inclusion, fat type and composition (i.e. contents of free and saturated fatty acids), and whether diets were formulated to be isoenergetic. The fact that the rations with fat supplements were isoenergetic and isonitrogenous may explain the absence of significant differences in animal performance. SM supplementation affected the composition of FA in meat of lamb. The SM addition decreased SFA (p < 0.01), SFA: PUFA (p < 0.01) and AI (p < 0.01) while increased MUFA (p < 0.001), PUFA (p < 0.001), CLA and DFA (p < 0.001). Palmitic acid (C16:0) reduced in SM treatment. Since C16 fatty acid has been introduced as a hypercholesterolemic FA, its reduction in meat and adipose tissue is beneficial to human health. Also, stearic acid (C18:0) (p < 0.05) decreased. Endogenous synthesis of MUFA in adipose tissues involves a reduction of C16:0 and C18:0 FA catalyzed by the ∆9 –desaturase activity. It is reported that ∆9 –desaturase expression is influenced by polyphenolic compounds (46). Also, the increase in cis-9 C18:1 proportion in meat of lambs fed SM diets can be explained by the high dietary cis-9 C18:1 level in SM groups, probably combined with slow ruminal biohydrogenation. Oleic acid (cis-9 C18:1) with stearic acids (C18:0) and palmitic (C16:0) to be the most abundant. Palmitic acid increases while oleic acid decreases blood cholesterol, and stearic acid has no effect. The cis-9 C18:1 reduce human LDL-cholesterol and increase HDL-cholesterol concentrations in blood, which result in lower risk of coronary problems. CLA nutrition has been shown to have anti-cancer, anti-obesity, anti-inflammatory, and anti-atherogenic effects, as well as positive effects on serum lipids.
Conclusion The results of this study indicated that meat FA composition of lambs can be improved from a human health perspective by inclusion of SM, as a rich source of PUFA. Addition of SM up to 12 % in lambs diet, increased the proportion of CLA, MUFA, PUFA, MUFA: SFA and PUFA:SFA ratio and decreased SFA and AI in meat. However, further investigation is needed to optimize the level of SM incorporation in animal diet.

کلیدواژه‌ها [English]

  • Digestibility
  • Fatty acids
  • Sesame meal
1-AOAC. 1990. Official Methods of Analysis. 14th ed. Arlington, VA, USA: Association of Official Analytical Chemists, MD.
2-Atti, N., M. Mahouachi, and H. Rouissi. 2006. The effect of spineless cactus (opuntia ficus-indica f. inermis) supplementation on growth, carcass, meat quality and fatty acid composition of male goat kids. Meat Science, 73(2):229-235.
3-Aurousseau, B., D. Bauchart, E. Calichon, D. Micol, and A. Priolo. 2004. Effect of grass or concentrate feeding systems and rate of growth on triglyceride and phospholipid and their fatty acids in the M. longissimus thoracis of lambs. Meat Science, 66(3): 531–541.
4-Bessa, R. J. B., P. V. Portugal, I. A. Mendes, and J. Santos-Silva. 2005. Effect of lipid supplementation on growth performance, carcass and meat quality and fatty acid composition of intramuscular lipids of lambs fed dehydrated lucerne or concentrate. Livestock Production Science, 96(2-3): 185–194.
5-Bessa, R. J. B., S. P. Alves, and J. Santos-Silva. 2015. Constraints and potentials for the nutritional modulation of the fatty acid composition of ruminant meat. Eur. European Journal of Lipid Science and Technolgy, 177(9): 1325–1344.
6-Boles, J. A., R. W. Kott, P. G. Hatfield, J. W. Bergman, and C. R. Flynn. 2005. Supplemental safflower oil affects the fatty acid profile, including conjugated linoleic acid, of lamb. Journal of Animal Science, 83(9): 2175–2181.
7-Chiliard, Y., A. Ferlay, J. Rouel, and G. Lamberet. 2003. A review of nutritional and physiological factors affecting goat milk lipid synthesis and lipolysis. Journal of Dairy Science, 86(5):1751-1770.
8-Desphande, S. S., U. S. Desphande, and D. K. Salunkhe.1996. In Y. H. Hui (5Ed.), Sesame oil, in bailey’s industrial oil and fat products. NewYork, 457–497.
9-Elleuch, M., S. Besbes, O. Roiseux, C. Blecker, and H. Attia. 2007. Quality characteristics of sesame seeds and by-products. Food Chemistry, 103(2):641-650.
10-Ferramosca, A., V. Savy, L. Conte, S. Colombo, A. W. C. Einerhand, and V. Zara. 2006. Conjugated linoleic acid and hepatic lipogenesis in mouse: role of the mitochondrial citrate carrier. Journal of Lipid Research, 47(9):1994-2003.
11-Folch, J., M. Lees, and G. S. Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissue. Journal of Biological Chemistry, 226(1):497–509.
12-Ghafari, H., M. Rezaeian, S. D. Sharifi, A. A. Khadem, and A. Afzalzadeh. 2016. Effects of dietary sesame oil on growth performance and fatty acid composition of muscle and tail fat in fattening Chaal lambs. Animal Feed Science and Technology, 220: 216–225.
13-Ghorbani, B., A. Taymoori-Yanesari, and A. Jafari-Sayyadi. 2016. Effects of replacement of sesame meal with soybean meal on intake, digestibility, rumen characteristics, chewing activity, performance, and carcass composition of lambs, Journal of Ruminant Research, 4(2):145-170. (In Persian).
14-Givens, D. I. 2005. The role of animal nutrition in improving the nutritive value of animal-derived foods in relation to chronic disease. Proceedings of the Nutrition Society, 64(3):395–402.
15-Haddad, S. G., and H. M. Younis. 2004. The effect of adding ruminally protected fat in fattening diets on nutrient intake, digestibility and growth performance of Awassi lambs. Animal Feed Science and Technology, 113(1-4): 61–69.
16-Harfoot, C.G., and G. P. Hazelwood. 1997. Lipid metabolism in the rumen. In: The Rumen Microbial Ecosystem. Elsevier Science Publishing, London, 382–426.
17-Hopkins, D. L., E. H. Clayton, T. A. Lamb, R. J. vandeven, G. Refshauge, M. J. Kerr, K. Bailes, and E. N. Ponnampalam. 2014. The impact of supplementing lambs with algae on growth, meat traits and oxidative status. Meat Science, 98(2): 135–141.
18-Horcada, A., G. Ripoll, M. Alcalde, C. Sanudo, A. Teixeira, and B. Panea. 2012. Fatty acid profile of three adipose depots in seven Spanish breeds of suckling kids. Meat Science, 92(2):89-96.
19-Hossain, M. M., M. A. Huq, M. Saadulah, and S. Akhter. 1989. Effect of supplementation of rice straw diets with sesame oil cake, fish meal and mineral mixture on dry matter digestibility in goats. Indian Journal of Animal Nutrition, 6(1): 44–47.
20-Hwang, L. S. 2005. Bailey's industrial oil and fat products. 6th ed, INC
21-Jaworska, D., M. Czauderna, W. Przybylski, and A. J. Rozbicka-Wieczorek. 2016. Sensory quality and chemical composition of meat from lambs fed diets enriched with fish and rapeseed oils, carnosic acid and seleno-compounds. Meat Science, 119: 185–192.
22-Kelly, M. L., J. R. Berry, D.A. Dwyer, J. M. Griinari, P. Y. Chouinard, M. E. Van Amburgh, and D.E. Bauman. 1998. Dietary fatty acid sources affect conjugated linoleic acid concentrations in milk from lactating dairy caws. The Journal of Nutrition, 128(5): 881-885.
23-Khalid, E. K., E. E. Babiker, and A.  E.  Tinay. 2003. Solubility and functional properties of sesame seed proteins as influenced by pH and/or salt concentration. Food Chemistry, 82(3): 361-366.
24-Khan Maher, M. A. 2002. Agro-industrial by-products as a potential source of livestock feed. International Journal of Agriculture & Biology, 4(2): 307-310.
25-Lock, A. L., and D. E. Bauman. 2004. Modifying milk fat composition of dairy cows to enhance fatty acids beneficial to human health. Lipids, 39(12):1197-1206.
26-Luna, P., A. Bach, M. Juárez, and M. A. delafuente. 2008. Influence of diets rich in flax seed and sunflower oil on the fatty acid composition of ewes milk fat especially on the level of conjugated linoleic acid, n-3 and n-6 fatty acids. International Dairy Journal, 18(1):99–107.
27-Mahmoud, A. E., and M. M. Bendary. 2014. Effect of whole substitution of protein source by nigella sativa meal and sesame seed meal in ration on Performance of growing lambs and calves. Global Veterinaria, 13(3): 391-396.
28-Manso, T., R. Bodas, T. Castro, V. Jimeno, and A. R. Mantecon. 2009. Animal performance and fatty acid composition of lambs fed with different vegetable oils. Meat Science, 83(3): 511–516.
29-Medeiros, E., R. Queiroga, M. Oliveira, A. Medeiros, M. Sabedot, M. Bomfim, and M. Madruga. 2014. Fatty acid profile of cheese from dairy goats fed a diet enriched with castor, sesame and faveleira vegetable oils. Molecules, 19(1): 992-1003.
30-Mekki, I., F. Camin, M. Perini, S. Smeti, H. Hajji, M. Mahouachi, and N. Atti. 2016. Differentiating the geographical origin of Tunisian indigenous lamb using stable isotope ratio and fatty acid content. Journal of Food Composition and Analysis, 53: 40–48.
31-Mir, P. S., T. A. McAllister, S. Scott, J. Aalhus, V. Baron, D. McCartney, E. Charmley, L. Goonewardene, J. Basarab, E. Okine, R. J Weselake, and Z. Mir. 2004. Conjugated linoleic acid-enriched beef production. The American Journal of Clinical Nutrition, 79(6):1207-1211.
32-Moazzami, A. A., R.E. Andersson, and A. Kamal-Edlin. 2006. HPLC analysis of sesaminol glucosides in sesame seeds. Journal of Agricultural and Food Chemistry, 54 (3): 633–638.
33-Moon, H. S., H. G. Lee, C. S. Chung, Y. J. Choi, and C. S. Cho. 2008. Physico-chemical modifications of conjugated linoleic acid for ruminal protection and oxidative stability. Nutrition & Metabolism, 5(1):16-28.
34-NRC, 2007. Nutrient Requirements of Small Ruminants: Sheep, Goats, Cervids, and New World Camelids. National Academy Press, Washington, DC.
35-Nudda, A., G. Battacone, M.G. Usai, S. Fancellu, and G. Pulina. 2006. Supplementation with extruded linseed cake affects concentrations of conjugated linoleic acid and vaccenic acid in goat milk. Journal of Dairy Science, 89(1): 277–282.
36-Obeidata, B. S., A.Y. Abdullaha, K. Z. Mahmouda, M. S. Awawdehb, N. Z. Al-beitawia, and F. A. Al-Lataifeh. 2009. Effects of feeding sesame meal on growth performance, nutrient digestibility, and carcass characteristics of Awassi lambs. Small Ruminant Research, 82(1):13–17.
37-Omar, J.A. 2002. Effect of feeding different levels of sesame oil cake on performance and digestibility of Awassi lambs. Small Ruminant Research,46(2-3):187-190.
38-Parvar, R., T. Ghoorchi, M. Shams Shargh. 2017. Influence of dietary oils on performance, blood metabolites, purine derivatives, cellulase activity and muscle fatty acid composition infattening lambs. Small Ruminant Research, 150: 22–29.
39-Saleh, S. A., and M. M. Amer. 2009. The Role of sesame seeds supplementation on lambs' growth and physiological performance. Journal of Radiation Research and Applied Sciences, 2(3) 623-639.
40-Santos-Silva, J., I. A. Mendes, P.V. Portugal, and R. Bessa. 2004. Effect of particle size and soybean oil supplementation on growth performance, carcass and meat quality and fatty acid composition of intramuscular lipids of lambs. Livestock Production Science, 90(2-3): 79–88.
41-Sanz Sampelayo, M. S., Y. Chilliard, P. Schmidely, and J. Boza. 2007. Influence of type of diet on the fat constituents of goat and sheep milk. Small Ruminant Research, 68(1-2): 42-63.
42-SAS Institute, 2002. STAT User's Guide: Statistics. Version 9.1. Statistical Analysis System Institute Inc., Cary, NC.
43-Scollan, N. D., N. J. Choi, E. Kurt, A. V. Fisher, M. Enser, and J. D. Wood. 2001. Manipulating the fatty acid composition of muscle and adipose tissue in beef cattle. British Journal of Nutrition, 85(1): 115–124.
44-Shirzadegan, K., and M. A. Jafari. 2014. The effect of different levels of sesame wastes on performance, milk composition and blood metabolites in Holsteinlactating dairy cows. International Journal of Advanced Biological and Biomedical Research, 2(4): 1296-1303.
45-Uzun, B., C. Arslan, M. Karhan, and C. Toker. 2007. Fat and fatty acids of white Lupin in comparison to sesame. Food Chemistry,102(1): 45.
46-Van Keulen, J., and B. Young. 1977. Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies. Journal of Animal Science, 44(2): 282-287.
47-Vasta, V., and R. J. B. Bessa. 2012. Manipulating ruminal biohydrogenation by the use of plants bioactive compounds. Dietary Phytochemicals and Microbes, Springer, Dordrecht, 263-284.
48-Yang, B., H. Chen, C. Stanton, R. R. Ross, H. Zhang, Y. Q. Chen, and W. Chen. 2015. Review of the roles of conjugated linoleic acid in health and disease. Journal of Functional Foods, 15: 314–325.
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