تأثیر استفاده از افزودنی های باکتریایی و اسانس رزماری، رازیانه و زنیان بر ترکیب شیمیایی، خصوصیات تخمیری، فراسنجه های تولید گاز و قابلیت هضم سیلاژ ذرت در شرایط برون تنی

نوع مقاله : علمی پژوهشی - تغذیه نشخوارکنندگان

نویسندگان

• فرشته مقصودلو ¹
• جواد بیات کوهسار ²
• فرزاد قنبری ²
• فاختک طلیعی ²

¹ دانشگاه گنبدکاووس

² گنبدکاووس

10.22067/ijasr.v8i4.52108

چکیده

مطالعه‌ای به منظور بررسی تأثیر استفاده از افزودنی باکتریایی و اسانس رزماری، رازیانه، زنیان در دو سطح 125 و 250 میکرولیتر بر کیلوگرم علوفه تازه بر ترکیب شیمیایی، قابلیت هضم برون‌تنی، فراسنجه‌های تولید گاز و ویژگی‌های تخمیر سیلاژ ذرت در قالب یک طرح کاملاً تصادفی انجام شد. علوفه‌های برداشت شده ذرت در سه تکرار در کیسه‌های پلاستیکی به صورت دستی فشرده و سیلو شدند. سیلوهای پر شده در دمای اتاق نگهداری و برای مدت 3، 7، 21 و 45 روز سیلو شدند. سیلاژهای ذرت تلقیح شده با افزودنی باکتریایی در روزهای 3 و 7 در مقایسه با تیمار شاهد دارای ماده خشک بالاتری بودند. غلظت کربوئیدرات‌های محلول در آب میان تیمارها مختلف بود. بالاترین و پایین‌ترین مقدار به ترتیب مربوط به تیمار رازیانه در سطح 125 میکرولیتر بر کیلوگرم در روز 3 و تیمار افزودنی باکتریایی در روز 45 پس از سیلو کردن بود. در بین تیمار‌ها، تیمار شاهد در روز 3 پس از سیلو کردن و تیمار رازیانه 250 میکرولیتر در روز 7 پس از سیلو کردن به ترتیب دارای بالاترین و پایین‌ترین پتانسیل تولید گاز بودند. سیلاژهای عمل آوری شده با اسانس زنیان در سطح 250 میکرولیتر و اسانس رازیانه در سطح 125 میکرولیتر در روز 21 به ترتیب بیشترین و کمترین توده میکروبی تولیدی را داشتند. به طور کلی، نتایج نشان داد که استفاده از اسانس‌ها در مقایسه با تیمار شاهد تأثیر قابل ملاحظه‌ای بر ارزش تغذیه‌ای سیلاژ ذرت نداشتند.

کلیدواژه‌ها

• اسانس
• ترکیب شیمیایی
• تلقیح باکتریایی
• تولید گاز
• قابلیت هضم برون‌تنی

20.1001.1.20083106.1396.8.4.4.3

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عنوان مقاله [English]

Effect of Bacterial Inoculation and Essential Oils of Rosemary, Fennel and Carum Copticum as Additive on Fermentation Process, Microbial Population and Nutritional Value of Corn Silage

نویسندگان [English]

• fereshteh maghsoudloo ¹
• javad Bayatkouhsar ²
• farzad ghanbari ²
• fakhtak taleai ²

¹ Gonbad Kavous University

² Gonbad Kavous University

چکیده [English]

Introduction In recent years the use of herbal products to prevent disease-causing agents including viruses, bacteria, fungi Pistaciavera are widely considered. They have a wide range of antimicrobial activity and a willingness to use them escalated. The aim of this study was to evaluate the effect of bacterial inoculation and essential oils of rosemary, fennel and carum copticum on chemical composition, gas production parameters and fermentation characteristics of corn silage in vitro.
Materials and Methods Whole corn plants were harvested at the medium dough stage of maturity (30% DM) and chopped with a conventional forage harvester under farm conditions to 3-5 cm of lengths. Representative forage samples (3 kg) of inoculated and untreated corn silage were packed manually, in triplicate, into plastic bags. Bacterial inoculants and essential oil diluted in deionized water and applied with a hand held sprayer while forage samples were stirred manually. A similar quantity of deionized water was sprayed on the control forage. The filled silos were stored at ambient temperature and allowed to ensile for 3, 7, 21 and 45 days. After designated ensiling times, silos were opened and the ensiled forage was mixed thoroughly and then were dried at a 60°C in oven for 48 h and then ground to pass through a 2 mm screen for later analysis.
Results and Discussion The chemical composition of samples was affected by different treatments (P

کلیدواژه‌ها [English]

• chemical composition
• Bacterial inoculation
• Gas production
• Essential oils
• in vitro digestibility

مراجع

1- Adsogan, D. J., B. N. Ziogas, and M. G. Polissiou. 2000. GC-MS analysis of essential oils from Greek aromatic plants and their fungitoxicity on Penicillum digitatum. Journal of Agriculture Food Chemistry, 48: 2576–2581.

2- AOAC .2003. Official Methods of Analysis. 15th edn. Association of Official Analytical Chemists, Washington, DC. USA.

3- Bayatkouhsar, J., R. Valizade., A. A. Naseriyan, and A. M. Tahmasebi., and R. Safari. 2009. Determine the nutritional value of citrus pulp using gas production. Third Congress of Animal Science, Ferdowsi University of Mashhad. (In Persian).

4- Benchhaar, C., H. V. Petie, R. Berthiaume, D. R. Ouellet, J. Chiquette, and P. Y. Chouinard. 2007. Effects of essential oils on digestion, ruminal fermentation, rumen microbial populations, milk production, and milk composition in dairy cows fed alfalfa silage on corn silage. Journal of Dairy Science, 90: 886-897.

5- Blummel, M., H. P. S. Makkar, and K. Becker. 1997. In vitro gas production: a technique revisited. Journal of Animal Physiology and Animal Nutrition, 77: 34-24.

6- Bolsen, K. K., D. R. Bonilla., G. L. Huck., M. A. Young, and R. A. Hart-Thakur. 1996. Effect of a propionic acid bacterial inoculant on fermentation and aerobic stability of whole-plant corn silage. Journal of Animal Science, 74 (1): 78-81.

7- Broderick, G.A., J.H. Kung. 1980. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Animal Science, 63:64–75.

8- Burt, S. 2004. Essential oils: their antibacterial properties and potential applications in foods– a review. Internatinal Journal of Food Microbiology, 94:223–253.

9- Calsamiglia, S., L. Castillejos, and M. Busquet. 2006. Alternatives to antimicrobial growth promoters in cattle. Pages 129–167 in Recent Advances in Animal Nutrition. P. C. Garnsworthy, and Journal of Wiseman, ed. Nottingham University Press, Nottingham, UK.

10- Chao, S. C. and D. G. Young. 2000. Screening for inhibitory activity of essential oils on selected bacteria, fungi and viruses. Journal of Essential Oil Research, 12: 639–649.

11- Chaves, A. V., J. Baah., Y. Wang., T. A. McAllistera, and C. Benchaar. 2012. Effects of cinnamon leaf, oregano and sweet orange essential oils on fermentation and aerobic stability of barley silage. Journal of Science Food Agriculther, 92: 906–915.

12- Deans, S. G. and G. Ritchie. 1987. Antibacterial properties of plant essential oils. Internatinal Journal of Food Microbioligy, 5:165-180.

13- Getachew, G., E. J. Depiters, and P. H. Robinson. 2002. In vitro gas production provides effective method for assessing ruminant feeds. California Agriculther, 58: 54-58.

14- Guenther, E. 1948. The essential Oils. D. Van Nostrand, New York.

15- Hedge, J. E., B. T. Hofreiter. 1962. Carbohydrate Chemistry 17. R. L. Whistle and J. N. Be Miller, Ed. Academic Press, New York.

16- Kalemba, D., A. Kunicka. 2003. Antibacterial and Antifungal Properties of Essential Oils. Current Medicinal Chemistry, 10: 813-829.

17- Kung, J. L. and R. E. Muck. 1997. Animal response to silage additives. Pages 200–210 in Proceedings of the Silage: Field to Feedbunk, North American Conference, Hershey, PA USA.

18- Kung, J. L., M. R. Stokes, and C. J. Lin. 2004. Silage additives. Pages 305–360 in Silage Science and Technology (Agronomy Series No. 42). D. R. Buxton, R. E. Muck, and H. J. Harrison, ed. American Society of Agronomy, Madison, WI.

19- Kung, J. L., P. Williams., R. J. Schmidt, and W. Hu. 2008. A blend of essential plant oils used as an additive to alter silage fermentation or used as a feed additive for lactating dairy cows. Journal of Dairy Science, 91: 4793–4800.

20- Kunkle, W. E., C. G. Chambliss., A. T. Adesogan, and M. B. Adjei. 2006. Silage harvesting, Storing and Feeding. University of Florida Online. Available: http://edis. Ifas.ufl.edu./publication.html.

21- Maherri-Ciss, N. 2008. Effect of Thyme essential oils (Thymus hyemalis and Thymus zygis) and Monensin on in vitro ruminal degradation and volatile fatty acid production. Journal of Agriculther and Food Chemistery, 54: 6598-6602.

22- Makkar, H. P. S. 2005. In vitro gas methods for evaluation of feeds containing phytochemicals. Animal Feed Science and Techmology, 123: 291-302.

23- Menke, K. H., L. Raab., A. Solewski., H. H. Steingass., D. Fritz, and W. Schneider. 1979. The estimation of the digestibility and metabolisable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro. Journal of Agriculture science, 93: 217-222.

24- Menke, K. H. L. and H. H. Steingass. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Journal of Animal Research and Development, 28: 7–55.

25- O’Hara, M. and K. Ohki. 1973. Studies of the mode of gas production in an artificial rumen and its application to the evaluation of feedstuffs. III. The mode of volatile fatty acid production, and its relation to the gas production rate. Japanese Journal of Zootechnology and Science, 44: 432-439.

26- Olivera, M. P .1998. Use of in vitro gas production technique to assess the contribution of both soluble and insoluble fraction on the nutritive value of forage. MSc Thesis. University of Aberdeen, Scotland.

27- Ørskov, E. R, and I. McDonald. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agriculture Science, 92: 499-503.

28- Rew, R. H. 1988. Stack Ensilage, Walter Scott, London.

29- Rowghani, E. and M. J. Zamiri. 2009. The effects of a microbial inoculant and formic acid as silage additives on chemical composition, ruminal degradability and nutrient digestibility of corn silage in sheep. Iranian Journal of Animal Science Research, 10: 2-27. (In Persian).

30- Sallam, S. M. A., S. M. A. Abdelgaleil., I. C. S. Bueno., M. E. A. Nassera., R. C. Araujo, and A. L. Abdalla. 2011. Effect of some essential oils on in vitro methane emission. Archives of Animal Nutrition, 65: 203–214.

31- SAS Institute. 2000. SAS User’s Guide: Statistics, Version 9.1 Edition. Cary, NC, USA.

32- Sivropoulou, A., E. Papanikolaou., C. Nikolaou., S. Kokkini., T. Lanaras, and M. Arsenakis. 1996. Antimicrobial and cytotoxic activities of Origanum essential oils. Journal of Agriculture and Food Chemistry, 44: 1202–1205.

33- Soltani Poor, M.A., M.B. Rezaee, and A. Moradshahi. 2004. Study on antimicrobial effects of essential oil of zhumeria majdae Rech. F & Wendelbo. Iranian Journal of Medicinal and Aromatic Plants, 20: 277-289. (In Persian).

34- Sommart, K., D. S. Parker., P. Rowlinson, and M. Wanapat. 2000. Fermentation characteristics and microbial protein synthesis in an in vitro system using cassava, rice straw and dried ruzi grass as substrates. Asian- Aust. Journal of Animal Science, 13: 1084-1093.

35- Taylor, C. C., N. J. Ranjit., J. A. Mills., J. M. Neylon, and J. L. Kung. 2002. The effect of treating whole-plant barley with Lactobacillus buchneri 40788 on silage fermentation, aerobic stability, and nutritive value for dairy cows. Journal of Dairy Science, 85: 1793–1800.

36- Theodorou, M. K., B. A. Williams. M .S. Dhanoa. A. B. McAllan, and J. France. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Scince and Technology, 48: 185–197.

37- Van Soest, P. J. and J. B. Robertson. 1979. Systems of analyses for evaluation of fibrous feed. Pages 49–60 in Proceedings of the International Workshop on Standardization of Analytical Methodology for Feeds W. J. Pigden., C. C. Baich and M. Graham, Eds. International Development Research Center, Ottawa, Canada.

38- Weinberg, Z. G., G. Szakacs., G. Ashbell, and Y. Hen. 2001. The effect of lactobacillus buchneri and lactobacillus plantarum, applied at ensiling on the ensiling fermentation and aerobic stability of wheat and sorghum silage. Journal of Industrial Microbiology and Biotechnology, 23: 218–222.

39- Wheeler, J. L. and C. Mulcahy. 1989. Consequences for animal production of cyanogenesis in sorghum and hay. Tropical Grasslands, 23: 193-202.