جایگزینی بخشی از یونجه جیره با برگ درخت عناب و تأثیر آن بر قابلیت هضم، برخی پارامترهای خونی و تخمیری شکمبه‌ای در بره‌های نر بلوچی

نوع مقاله : علمی پژوهشی - تغذیه نشخوارکنندگان

نویسندگان

1 دانشکده کشاورزی و منابع طبیعی، دانشگاه بیرجند، بیرجند، ایران

2 گروه تغذیه دام و طیور، دانشکده کشاورزی و منابع طبیعی بیرجند، بیرجند، ایران.

3 گروه تغذیه دام و طیور، دانشکده کشاورزی و منابع طبیعی بیرجند، بیرجند، ایران

چکیده

جهت بررسی جایگزینی بخشی از یونجه جیره با برگ درخت عناب و تأثیر آن بر قابلیت هضم، برخی پارامترهای خونی و تخمیری شکمبه‌ای در بره‌های نر بلوچی، آزمایشی در قالب سه تیمار (0، 6 و 12 درصد برگ درخت عناب) بر پایه طرح کاملا تصادفی انجام شد. جایگزینی برگ درخت عناب با بخشی از یونجه جیره، تأثیر معنی‌داری بر میانگین مصرف خوراک روزانه، قابلیت هضم مواد مغذی، برخی پارامترهای خونی و تخمیری شکمبه داشت، به‌طوری‌که pH مایع شکمبه، قابلیت هضم پروتئین خام، گلوکز، نیتروژن اوره‌ای خون در تیمارهای حاوی 6 و 12 درصد برگ درخت عناب، کاهش معنی‌داری نسبت به شاهد نشان داد. در میان تیمارها، بیشترین مقدار کلسترول،HDL و LDL مربوط به جیره حاوی 12 درصد برگ عناب بود. نتایج حاصل از این آزمایش نشان داد که استفاده از جیره حاوی 6 درصد برگ عناب، اثرات منفی بر قابلیت هضم مواد مغذی، فراسنجه­های تخمیری شکمبه‌ای و خونی نداشت.

کلیدواژه‌ها


عنوان مقاله [English]

Replacement of part of diet hay with leaf of jujube tree and its effect on digestibility, some parameters of blood and fermentation of rumen in lambs of Baluchi

نویسندگان [English]

  • Hossein Hassan Pour Taghi Abad 1
  • Moslem Bashtani 2
  • Mohammed reza Asghari 3
1 Knowledge of Animal and Poultry Nutrition Department, Faculty of Agricultural and Natural Resources, University of Birjand,
2 Animal and Poultry Nutrition Department, Faculty of Agricultural and Natural Resources, University of Birjand,
3 The Coach Animal and Poultry Nutrition Department, Faculty of Agricultural and Natural Resources, University of Birjand,
چکیده [English]

Introduction[1]In most part of Iran, low rainfall and availability of water resources are the major limitations to the farmer.Feed represents a major proportion of the overall production cost for livestock industry across the world. On the other hand, availability of good quality and unadulterated conventional feed all year round is a major constraint in livestock production. Thus, proper use of expensive agricultural by-products is important goal in livestock production. Therefore, one way to overcome the limitation of forage resources and the high cost of livestock feed is using agricultural by products such as plant leaves or other unconventional sources of the feed. Jujube is a short tree with a height of 2 to 8 meters and sometimes up to 12 meters and is very resistant to drought. The cultivation area of jujube in Iran is about 3,000 hectares, more than 95% of which (about 2850 ha) is in southern Khorasan and 4,200 tons of jujube is produced.  The leaves are readily eaten by camels, cattle and goats and are considered nutritious. Analyses has shown the following constituents (% dry weight): crude protein, 12.9-16.9; fat, 1.5-2.7; fiber, 13.5-17.1; N-free extract, 55.3-56.7; ash, 10.2-11.7; calcium, 1.42-3.74; phosphorus, 0.17-0.33; magnesium, 0.46-0.83; potassium, 0.47-1.57; sodium, 0.02-0.05; chlorine, 0.14-0.38; Sulphur, 0.13-0.33%. The goal of the experiment was to investigate the effects of different levels of jujube leaves on yield, digestibility and fermentation characteristics of Baluchi male lambs.
Materials and Methods Jujube leaves were analyzed for dry matter (DM), organic matter (OM), crude protein (CP) and ether extract (EE) contents using standard procedures (AOAC, 2005). Neutral detergent fiber (NDF) was analyzed according to Van Soest et al. (1991), total phenols (TP) and total tannins (TT). Twenty-four Baluchi male lambs with the age of 140±10 days were assigned to a completely randomized design with 3 treatments and 8 replicates for 75 days 3 dietary treatments and 8 replications in a completely randomized design. Lambs were grouped based on their age and weight. Weight and experimental trail lasted for 75 days. Diets were formulated based on SRNS (1.9.6069) guidelines. Concentrate diet and dried alfalfa hay were used in the experiment. All lambs were fed ad libitum twice daily at 08:00 and 20:00 h with a Total Mixed Ration (TMR) of 30% forage (Alfalfa) and 70% concentrate. The dietary treatments were 1) control (without jujube leaves), 2) 6% jujube leaves, and 3) 12% jujube leaves. An adaptation period of 15 days and an experimental period of 75 days were considered for this experiment. During the experimental period, feed distributed to each animal and corresponding ort were recorded daily. Representative samples of feed and ort were bulked for subsequent analyses. Animal BW was recorded at every 10 days of the experiment after 16 h fasting, to calculate weight gain. In order to directly measure the apparent digestibility of nutrients during 10 days at the end of the experiment, samples were collected from the feeds and their residues and the fecal was collected in the last 7 days. Sampling of rumen fluid was done on days 25th, 50th and 75th of the experimental period, 2 hours after ingestion in the morning using an esophagus tube and its pH was determined immediately. For determination of nutrients digestibility, the amount of feed intake, ort and fecal excretion were recorded. On the 25th, 50th and 75th days of the experimental period, approximately 10 mL of blood was collected from the jugular vein of each. Blood was collected in a tube and directly centrifuged at 3000 rpm for 10 min at room temperature. The supernatant (serum) was collected and frozen at −20 °C pending further analysis. Blood serum metabolites, glucose, HDL, LDL, cholesterol and blood urea nitrogen were measured.
Results and Discussion The results of the experiment showed the amount of dry matter, organic matter, crude protein, ash, ether extract, NDF and ADF of jujube leaves were 93.93,88.70, 7.61, 11.30, 1.8, 41.50 and 13.50%, respectively. Also, total phenol, total tannin and condensed tannin were reported to be 4.7, 3.1 and 0.48% respectively. The results of the experiment showed that the use of different levels of jujube leaves had no significant effect on mean daily weight gain and feed conversion ratio, but in jujube leaves treatments, the average daily feed intake was significantly decreased.The digestibility of crude protein in jujube leaves treatments significantly decreased compared to control. The digestibility of dry matter, organic matter, NDF and ADF did not have a significant effect on the treatments (P> 0.05), but the digestibility of crude protein in jujube leaves treatments significantly decreased compared to control (p < 0.05). The pH of the ruminal fluid decreased linearly with increasing levels of jujube leaves, but did not have a significant effect on ruminal ammonia nitrogen concentration. Blood glucose concentration, HDL, LDL, cholesterol and blood urea nitrogen were significantly affected by the treatments.
Conclusion According to results of this experiment, the use of jujube leaves in the diet due to the presence of tannin reduced feed intake, digestibility of crude protein, blood glucose concentration and blood urea nitrogen and did not have a negative effect on the pH of the rumen fluid.

کلیدواژه‌ها [English]

  • Chemical composition
  • Fattening lambs
  • Jujube leaves
  • Performance
  • Tannins
1-         AOAC (Association of Official Analytical Chemists). 2002. Official Methods of Analysis. 18th ed. AOAC International, Gaithersburg, DC.
2-         AOAC (Association of Official Analytical Chemists). 2002. Official Method of Analysis. 17th ed. AOAC, Arlington, VA. Pp: 120-155.
3-         Barry, T. N., and W. C. Mc Nabb. 1999. The implication of condensed tannins on the nutritive value of temperate forages fed to ruminants. British Journal of Nutrition, 81:263-272.
4-         Bashtani, M., M. R. Tehrani., A. A. Naserian., M. H. Fathi., and F. Ganji. 2013. The effect of different levels of jujube leaves on feed intake and blood metabolites dairy goats. Iranian Journal of Animal Science Research, 5(2):157-163. (In Persian).
5-         Ben Salem, H., H. P. S. Makkar., A. Nefzaoui., L. Hassayoun., and S. Abidi. 2005. Benefit from the association of small amounts of tannin-rich shrub foliage (Acacia cyanophylla Lindl.) with soybean meal given as supplements to Barbarian sheep fed on oaten hay. Animal Feed Science and Technology, 122:173-186.
6-         Broderick, G. A., and J. H. Kang. 1980. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science, 63:64-75.
7-         Bunyeth, H. 2005. Cassava foliage as supplement for goats fed Paragrass (Brachiaria mutica) in full confinement, or with grazing in semi-confinement. MSc. Thesis. Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Uppsala, Sweden.
8-         Carulla, J. E., M. Kreuzer., A. Machmüller., and H. D.  Hess. 2005. Supplementation of Acacia mearnsii tannins decreases methanogenesis and urinary nitrogen in forage-fed sheep. Australian Journal of Agricultural Research, 56:961-970.
9-         Chaves, A. V., K. Stanford., M. E. R. Dugan., L. L. Gibson., T. A. McAllister., F. Van Herk., and C. Benchaar. 2008. Effects of cinnamaldehyde, garlic and juniper berry essential oils on rumen fermentation, blood metabolites, growth performance and carcass characteristics of growing lambs. Livestock Science, 117:215-224.
10-      Cheema, U. B., J. I. Sultan., A. Javaid., P. Akhtar., and M. Shahid. 2011. Chemical composition, mineral profile and in situ digestion kinetics of fodder leaves of four native trees. Pakistan Journal of Botany, 43:397-404.
11-      Frutos, P., M. Raso., G. Hervás., A. R. Mantecón., V. Pérez., and F. J. Giráldez. 2004. Is there any detrimental effect when a chestnut hydrolysable tannin extract is included in the diet of finishing lambs? Animal Research, 53:127-136.
12-      Givens, D. I., E. Owen., R. F. E. Auford., and H. M. Omend. 2000. Forage evaluation in ruminant nutrition, CABI publishing.
13-      Hristov, A. N., M. Ivan., L. M. Rode., and T. A. McAllister. 2001. Fermentation characteristics and rumen ciliate protozoal populations in cattle fed medium or high barley based diets. Journal of Animal Science, 79:515-524.
14-      Khafipour, E., D. O. Krause., and J. C. Plaizier. 2009. Alfalfa pellet induced subacute ruminal acidosis in dairy cows increases bacterial endotoxin in the rumen without causing inflammation. Journal of Dairy Science, 92:1712–1724.
15-      Kogmanila, D., and C. R. Preston. 2007. Chemical composition digestibility and intake characteristics of some tropical foliage species used for goats. http:/www.mekarn Regional conference 2007/ matching livestock system with available resources.
16-      Kondo, M., K. Kita., and H. O.  Yokota. 2004. Feeding value to goats of whole-crop oat ensiled with green tea waste. Animal Feed Science and Technology, 113:71-81.
17-      Krueger, W. K., H. Gutierrez-Banuelos., G. E. Carstens., B. R. Min., W. E. Pinchak., R. P. Gomez., R. C. Anderson., N. A. Krueger., and T. D. A. Forbes. 2010. Effects of dietary tannin source on performance, feed efficiency ruminal fermentation, and carcass and non-carcass traits in steers fed a high-grain diet. Animal Feed Science and Technology, 159(1-2):1-9.
18-      Liu, J. X., B. Yao., J. Q. Yu., and Z. Q. Shi. 2001. Effect of mulberry leaves to replce rapeseed meal on performance of sheep feeding on ammoniated rice straw diet. Small Ruminant Research, 39:131-136.
19-      Makkar, H. P. S. 2003. Effects and fate of tannins in ruminant animal, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feed. Small Ruminant Research, 49:241-256.
20-      Makkar, H. P. S., N. K. Borrowy, and K. Becker. 1992. Quantitation of polyphenols in animal feedstuffs. Proc. XVIth Int. Conf of Groupe Polyphenol, Lisbon. 13-17 July.
21-      Mathison, G. W., and L. P.  Milligan. 1971. Nitrogen metabolism in sheep. British Journal of Nutrition, 25(3):351-366.
22-      McDonald, P., R. A. Edwards, J. F. D. Greenhalgh, C. A., Morgan, L. A., Sinclair, and R. G.  Wilkinson. 2010. Animal Nutrition (7th ed.). Pearson Prentice Hall press.
23-      Min, B. R., G. T. Attwood, W. C. McNabb, A. L. Molanb, and T. N. Barry. 2005. The effect of condensed tannins from Lotus corniculatus on the proteolytic activities and growth of rumen bacteria.Journal of Animal Feed Science, 121:45–58.
24-      Morton, J. 1987. In fruits of warm climates. Indian Jujube. P: 272-275.
25-      Nath, K., and O. N. Nsand Singh. 1969. Utilization of Zizphus nummularia leaves by three breeds of sheep. Australian Journal Agriculture Research, 20:1137-1142.
26-      Patra, A. K, and J. Saxena. 2010. A new perspective on the use of plant secondary metabolites to inhibit methanogenesis in the rumen. Phytochemistry, 71(11-12):1198–1222.
27-      Perez-Maldonado, R. A., and B. W. Norton. 1996. The effects of condensed tannins from Desmodiumintortum and Calliandracalothyrsusonprotein and carbohydrate digestion in sheep and goats. British Journal of Nutrition, 76:515-533.
28-      Rahimi, A., A. Naserian, R. Valizadeh, and A.  Tahmasbi. 2013. Effect of alfalfa replacement with different levels of pistachio hull on feed consumption, nutrient digestibility, rumen fermentation metabolites, blood metabolites and nitrogen balance in balouchi male lamb. Iranian Journal of Animal Science Research, 5:190-200. (In Persian).
29-      Ramin, A. G., A. Aghazadeh, and T.  Karamian. 2007. Evaluate the relationship between dietary protein and energy with milk urea and lactose, and blood glucose and urea in lactating ewes. Iranian Veterinary Journal, 4(3):24-32.
30-      Reed, J. D. 1995. Nutritional toxicology of tannins and related polyphenols in forage legumes. Journal of Animal Science, 73:1516-1528.
31-      Reed, J. D., H. Soller, and Woodward, A. 1990. Fooder tree and straw diets for sheep: Intake, digestibility and the effect of phenolics on nitrogen utilization. Animal feed Science and Technology, 30:39-50.
32-      Roseler, D. K., J. D. Fergosen, C. J. Sniffen, and J. Herrema. 1993. Dietary protein degradability efficiency on plasma and milk and protein nitrogen in Holestein cow. Journal of Dairy science, 76:525-534.
33-      SAS. 2009. SAS Users guide: Statistics. Version 9.2 SAS Institute Inc., Cary, NC.
34-      Seeram, N. P., Y. Zhang, J. D. Reed, C. G. Krueger, and J. Vaya. 2006. Pomegranate phytochemical. CRC press, Taylor and Francis Group, Boca Raton, FL.
35-      Sinclair, L. A., K. J. Hart, R. G. Wilkinson, and J. A.  Huntington. 2009. Effects of inclusion of whole-crop pea silages differing in their tannin content on the performance of dairy cows fed high or low protein concentrates. Livestock Science, 124:306-313.
36-      Sreerangaraju, G., U. Krishnamoorthy, and M. M. Kailas. 2000. Evaluation of Bengal gram (Cicerarietinum) husk as a source of tannin and its interference in rumen and post-rumen nutrient digestion in sheep. Animal Feed Science and Technology, 85:131-138.
37-      Valizadeh, R., A. Salahi, M. Mahmoudi Abyaneh, and M. Salemi. 2016. The effect of replacing different levels of bulls with palatinate on the performance and health of Baluchi lambs. Iranian Journal of Animal Science Research, 7(4):406-412. (In Persian).
38-      Vansoest, P. J., J. B. Robertson, and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and non  starch polysacharides in relation to animal nutrition. Journal of Dairy Science, 74:35-83.
39-      Waghorn, G. C., I. D. Shelten. W. C.  Mc Nabb, and S. N. Mc Catdhern. 1994. Effects of undensed tamerin Lotus pedan culatus on nutritive value for sheep. 2. Nitrogenous aspects. Journal of Agriculture Science, 123: 109-119.
40-      Yildiz, S., I. Kaya, Y. Unal, D. Aksu Elmali, S. Kaya, M. Cenesiz, M. Kaya, and A.  Oncuer. 2005. Digestion and body weight change in Tuj lambs receiving oak (Quercus hartwissiana) leaves with and without PEG. Animal Feed Science and Technology, 122:159-172.
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