اثر مخمر اتولیز شده بر عملکرد تولید و کیفیت تخم‌مرغ، جمعیت میکروبی و ریخت‌شناسی روده مرغان تخم‌گذار

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.

2 مدیرعامل شرکت کاوشگر سپهر جوان، دزفول، ایران.

چکیده

به‌منظور مطالعه تأثیر مخمر اتولیز شده بر عملکرد، کیفیت تخم‌مرغ، جمعیت میکروبی سکوم و فراسنجه‌های هیستومورفومتری روده مرغان تخم‌گذار، آزمایشی با استفاده از 192 قطعه مرغ تخم‌گذار سویه‌های‌لاین در سن 64 هفتگی به‌مدت 10 هفته انجام شد. تیمارهای آزمایشی شامل سطوح مختلف مخمر پری‌ویتا: شاهد (بدون مکمل)، 250 سی‌سی در 1000 لیتر آب، 500 سی‌سی در 1000 لیتر آب (پیشنهاد شرکت)، 750 سی‌سی در 1000 لیتر آب بود که در قالب طرح کاملاً تصادفی با 8 تکرار اجرا شد. نتایج این پژوهش نشان داد که استفاده از مخمر اتولیز شده نتوانست فراسنجه­های عملکردی پرندگان را تحت تأثیر قرار دهد. اگرچه در سطوح 500 و 750 سی‌سی در 1000 لیتر آب به‌طور معنی‌داری جمعیت لاکتوباسیل افزایش و جمعیت کلی فرم‌های سکوم کاهش نشان داد. پرندگان مصرف‌کننده سطح 750 سی‌سی مخمر در هزار لیتر آب، ارتفاع پرز با‌لاتر و نسبت ارتفاع پرز به عمق کریپت بیشتر در دوزادهه در مقایسه با شاهد و سطح 250 سی‌سی مخمر در هزار لیتر آب داشتند. نتایج نشان داد که افزودن مخمر اتولیز شده پری‌ویتا در سطوح 500 و 750 باعث افزایش جمعیت لاکتوباسیل و کاهش کلی فرم و سطح 750 مخمر باعث کاهش جمعیت اشرشیاکولی سکوم و افزایش طول پرزها و نسبت ارتفاع پرز به عمق کریپت در ناحیه دوازدهه بدون تأثیر بر فراسنجه‌های کمّی و کیفی تخم‌مرغ در مرغان تخم‌‌گذار شد.
 

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of Autolyzed Yeast on Performance, Egg Quality, Microbial Population and Intestinal Morphology of Laying Hens

نویسندگان [English]

  • Smayyeh Salari 1
  • Karim Javidaneh 2
1 Department, of Animal Science, Facultyof Animal Science and Food Technology Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran,
2 Chairman of Executive Committee, Kavoshgar Sepehr Javan Company, Dezful, Iran
چکیده [English]

Introduction: Antibiotics have been routinely supplemented in diets of poultry to maintain their health, reduce stress, and enhance productivity. However, due to the development of resistance of bacteria to antibiotics and the possibility of these pathogens to be zoonotic, the use of various antibiotic products in livestock and poultry production is gradually being banned around the world. With the possibility of further ban in more regions of the world, research interest into alternatives to in-feed antibiotics has increased. One alternative to in-feed antibiotics that has gained research interest for use in poultry is yeast (Saccharomyces cerevisiae). Yeast in both probiotic (live) and prebiotic (dead) forms has been reported to provide several benefits to both healthy of animals, including poultry. The ability of yeast and its components to act as growth promoter could be associated with different mechanisms that it exhibits individually or synergistically. For instance, yeasts have been reported to favor the proliferation of beneficial microbes by serving as substrates for these microbes in the gut. These beneficial microbes, such as Lactobacillus, have been reported to improve gut health as well as exhibit growth-promoting effects in broiler chickens. Specifically, yeast cell wall that is extracted from whole yeast consists mainly of α-mannans and β-1–3-glucans, which are reported to prevent or eliminate bacterial infections.
Materials and Methods: In order to study various levels of autolyzed yeast (Privita) on performance, quality characteristics of egg, cecal microbiology and intestinal histomorphology of laying hens (Hy-line W-36) (64 weeks), an experiment was done with 192 birds for 10 weeks. Treatments were various levels of autolyzed yeast (control, 250 ml/1000l, 500 ml/1000l, and 750 ml/1000l) that conducted in completely randomized design with 8 replications. Egg production (EP) and egg weight (EW) were recorded daily and feed intake (FI) and feed conversion ratio (FCR) were evaluated weekly. Quality characteristics of eggs were evaluated 2 times per period. Iintestinal histomorphology was determined at the end of experiment. At the end of the study, one birds per replicate were killed by cervical dislocation and blood was drawn from the jugular vein. Serum was separated after centrifugation at 4500 g and 4°C for 10 min, and frozen at −20°C until further analysis was conducted for blood biochemical parameters. Serum samples were analysed for concentrations of low-density lipoprotein (LDL), cholesterol, and triglycerides using standard kits (Zist Shimi, Tehran, Iran) with an autoanalyser (Autolab PM 4000; Medical System, Rome, Italy). Then, caecal digesta (1 g) from each bird were aseptically transferred into 9 ml of sterile saline solution and serially diluted. Lactobacilli, Coliforms, and E.Coli were grown on Rogosa–Sharpe agar, MacConkey Agar, and Eosin Methylene Blue Agar, respectively. Plates for Lactobacillus were incubated anaerobically for 48 h at 37 °C. Microbial populations for E. coli and Coliforms were counted after aerobic incubation at 37°C for 24 hours. All samples were plated in duplicate.
Results and Discussion: This study results showed that addition of autolyzed yeast could not change performance parameters. Yolk color was highest at 250 ml/1000l which had a significant difference with 750 ml/1000l (P <0.05). Addition of autolyzes yeast at levels of 500 and 750 ml/1000l significantly increased cecal Lactobacillus and decreased Coliforms of cecum (P < 0.05). Addition of autolyzed yeast at level of 750 ml/1000l significantly decreased cecal Ecoli compared to the other treatments (P < 0.05). The possible reason for the observed response could be the ability of the yeast to maintenance of normal gut microflora as well as preventing the proliferation of pathogenic microbes. This action may partly contribute to increased nutrient digestibility, reduced competition for nutrients, increased nutrient utilization as well as absorption. Villus height of the e duodenum increased in layers fed on 750 ml/1000l compared to the birds fed 250 ml/1000l (P < 0.05). While crypt depth and villus height-to-crypt depth ratios of the duodenum in birds fed 750 ml/1000l compared to the birds 250 ml/1000l decreased and increased, respectively. Crypt depth of jejunum significantly decreased in birds fed highest level of autolyzed yeast compared to the other treatments.
Conclusion: Results of this experiment showed that supplementation of autholyzed yeast could not significant change in qualitative and quantitative parameters. But, levels of 500 and 750 ml/1000l autholyzed yeast increased cecal microbial of Lactobacillus and decreased Coliform and the level of 750 ml/1000l decreased Ecoli of cecum and increased villus height and villus height-to-crypt depth ratios of the duodenum.
 
 
 
 
 

کلیدواژه‌ها [English]

  • Microbial population
  • Histomorphometry
  • Laying hen
  • Yeast
  1. Adhikari, P. A., & Kim, W. K. (2017). Overview of prebiotics and probiotics: focus on performance, gut health and immunity–A review. Animal Science, 4, 949–966. https://doi.org/10.1515/aoas-2016-0092.
  2. Adhikari, P. A., Cosby, D. E., Cox, N. A., Franca, M. S. S., Williams, M., & Gogal Jr, R. M. (2018). Effect of dietary fructooligosaccharides supplementation on internal organs Salmonella colonization, immune response, ileal morphology, and ileal immunohistochemistry in aying hens challenged with Salmonella enteritidis. Poultry Science, 97, 2525–2533. https://doi: 10.3382/ps/pey101.
  3. Ahiwe, E. U., Abdallh, M. E., Chang'a, E. P., Al-Qahtani, M., Omede, A. A., Graham, H., & Iji, P.A. (2019). Influence of autolyzed whole yeast and yeast components on broiler chickens challenged with salmonella lipopolysaccharide. Poultry Science, 98(12), pp.7129-7138. https://doi.org/10.3382/ps/pez452.
  4. Bortoluzzi, C., Barbosa, J. G. M., Pereira, R, Fagundes, N. S., Rafael, J. M and Menten, J. F. M. (2018). Autolyzed yeast (Saccharomyces cerevisiae) supplementation improves performance while modulating the intestinal immune system and microbiology of broiler chickens. Frontiers in Sustainable Food Systems 2:85. https://doi.org/10.3389/fsufs.2018.00085.
  5. Botsoglu, N. A. & Fletouris, D. J. (2001). Drug resistant in foods. Pharmacology, Food Safety and Analysis. NewYork, Marcel Dekker, Inc. 541-548.
  6. Çabuk, M., Alçiçek, A., Bozkurt, M. & İmre, N. (2003). Antimicrobial properties of the essential oils isolated from aromatic plants and using possibility as alternative feed additives. II. National Animal Nutrition Congress. 18-20 September, Konya, Turkey. pp. 184-187.
  7. Davis, M. E., Maxwell, C. V., Erf, G. F., Brown, D. C., & Wistuba, T. J. (2004). Dietary supplementation with phosphorylated mannans improves growth response and modulates immune function of weanling pigs. Animal Science. 82, 1882–1891. https://doi: 10.2527/2004.8261882x.
  8. Hashim, M., Fowler, J., Haq, A., & Bailey, C.A., (2013). Effects of yeast cell wall on early production laying hen performance. Journal of Applied Poultry Research, 22(4), pp.792-797. https://doi.org/10.3382/japr.2012-00712.
  9. Iji, PA., Saki, A., & Tivey, DR. (2001). Intestinal development and body growth of broiler chicks on diets supplemented with non-starch polysaccharides. Animal Feed Science and Technology, 89, 175-188. https://doi.org/10.1016/S0377-8401(00)00223-6.
  10. Jaehrig, S.C., Rohn, S., Kroh, L.W., Wildenauer, F.X., Lisdat, F., Fleischer, L.G. and Kurz, T. (2008). Antioxidative activity of (1→ 3), (1→ 6)-β-d-glucan from Saccharomyces cerevisiae grown on different media. LWT-Food Science and Technology, 41(5), pp.868-877. https://doi.org/10.1016/j.lwt.2007.06.004.
  11. Koiyama, N. T. G., Utimi, N. B. P., Santos, B. R. L., Bonato, M. A., Barbalho, R., Gameiro, A. H., Araújo, C. S. S., & Araújo, L.F. (2018). Effect of yeast cell wall supplementation in laying hen feed on economic viability, egg production, and egg quality. Journal of Applied Poultry Research, 27(1), 116-123. https:// http://10.3382/japr/pfx052.
  12. Leeson, S., & Summers, J. D. (2008). Commercial Poultry Nutrition. 3rd Nottingham University Press, England.
  13. Martínez, B. F., Contreras, A. A., & González, E. Á. (2010). Use of Saccharomyces cerevisiae cell walls in diets for two genetic strains of laying hens reared in floor and cages. International Journal of Poultry Science, 9, 105-108. https:// DOI: 3923/ijps.2010.105.108.
  14. Masouri, L., Salari, S., Sari, M., Tabatabaei, S., & Masouri, B. (2017). Effect of feed supplementation with Satureja khuzistanica essential oil on performance and physiological parameters of broilers fed on wheat- or maize-based diets. British Poultry Science, 4, 425-434. https://DOI: 10.1080/00071668.2017.1327701 .
  15. Mateo, E. D., Dave, R. I., & Stein, H. H. (2004). Effect of supplemental nucleosides for newly weaned pigs. Animal Science, 82(Suppl. 2), 71.
  16. Mohan, B., Kadirvel, M., Bhaskaran, M., & Natarajan, A. 1995. Effect of probiotic supplementation on serum/ yolk cholesterol and on egg shell thickness in layers. British Poultry Science, 36, 799–803. https://doi.org/10.1080/00071669508417824.
  17. Nursoy, H., Kaplan, O., OGUZ, M., & Yilmaz, O. (2004). Effects of varying levels of live yeast culture on yield and some parameters in laying hen diets. Indian Veterinary Journal, 81, 59-62.
  18. Parks, C. W., Grimes, J. L., Ferket. P. R., & Fairchild, A. S. (2001). The effect of mannanoligosaccharides, bambermycins, and virginiamycin on performance of large white male market turkeys. Poultry Science, 80, 718–723.https:// DOI: 1093/ps/80.6.718 .
  19. Parsaie, S. J., Shariatmadari, F., Zamiri, M. J., & Khajeh, K. (2007). Influence of wheat-based diets supplemented with xylanase, bile acid and antibiotics on performance, digestive tract measurements and gut morphology of broilers compared with a maizebased diet. British Poultry Science, 48, 594–600. https://doi: 10.1080/00071660701615788.
  20. Rezaeipour, V., Fononi, H., & Irani, M. (2012). Effects of dietary L-threonine and Saccharomyces cerevisiae on performance intestinal morphology and immune response of broiler chickens. Animal Sciences, 42, 266-273. https://hdl.handle.net/10520/EJC127732.
  21. Ricke, S.C. (2018). Focus: nutrition and food science: Impact of prebiotics on poultry production and food safety. The Yale Journal of Biology and Medicine, 91(2), 151.
  22. Russell, J. B. (1992). Glucose toxicity and inability of Bacteroides ruminicola to regulate glucose transport and utilization. Applied and Environmental Microbiology, 58(6), 2040-2045. https://doi: 1128/aem.58.6.2040 2045.1992
  23. Santin, E., Maiorka, A., Macari, M., Grecco, M., Sanchez, J. C., Okada, T. M., & Myasaka, A. M. (2001): Performance and intestinal mucosa development of broiler chickens fed diets containing Saccharomyces cerevisae cell wall. Journal of Applied Poultry Research, 10, 236–244. https://doi.org/10.1093/japr/10.3.236.
  24. SAS Institute Inc. (2003). SAS/STAT User’s Guide Version 9. SAS Institute Inc., Cary, NC.
  25. Shang, Y., Kumar, S., Thippareddi, H., & Kim W. K. (2018). Effect of dietary fructooligosaccharide (FOS) supplementation on ileal microbiota in broiler chickens. Poultry Science, 97, 3622–3634. https://doi: 10.3382/ps/pey131.
  26. Shang, Y., Regassa, A., Kim, J. H., & Kim, W. K. (2015). The effect of dietary fructooligosaccharides supplementation on growth performance, intestinal morphology, and immune response in broiler chickens challenged with Salmonella enteritidis Poultry Science, 94, 2887–2897. https://doi: 10.3382/ps/pev275.
  27. Shao, Y., Guo, Y., & Wang, Z. (2013). β-1,3/1,6-Glucan alleviated intestinal mucosal barrier impairment of broiler chickens challenged with Salmonella enterica serovar typhimurium. Poultry Science, 92, 1764–1773. https://doi: 10.3382/ps.2013-03029.
  28. Spring, P., Wenk, C., Dawson, K.A., & Newman, K.E. (2000). The effects of dietary mannaoligosaccharides on cecal parameters and the concentrations of enteric bacteria in the ceca of salmonella-challenged broiler chicks. Poultry Science, 79(2), 205-211. https://doi: 10.1093/ps/79.2.205.
  29. USDA Egg-Grading Manual. (2000). Agricultural Handbook Number 75. USDA Agricultural Marketing Service, Washington, DC. 56 p.
  30. VanLeeuwen, P., Mouven, J. M. V. M., VanderKlis, J. D., & Verstegen, M. W. A. (2004). Morphology of the small intestinal mucosal surface of broiler in relation to age, diet formulation, small intestinal microflora and performance. British Poultry Science, 45, 41-48. https://doi: 10.1080/00071660410001668842.
  31. Viveros, A., Chamorro, S., Pizarro, M., Arija, I., Centeno, C., & Brenes, A. (2011). Effects of dietary polyphenol-rich grape products on intestinal microflora and gut morphology in broiler chicks. Poultry Science, 90, 566-578. https://doi.org/10.3382/ps.2010-00889.
  32. Xu, Z. R., Hu, C. H., Xia, M. S., Zhan, X. A., & Wang, M. Q. (2003). Effects of dietary fructooligosaccharide on digestive enzyme activities, intestinal microflora and morphology of male broilers. Poultry Science, 82, 1030–1036. https://doi: 10.1093/ps/82.6.1030.
  33. Yalçin, S., Özsoy, B., & Erol, H. (2008). Yeast culture supplementation to laying hen diets containing soybean meal or sunflower seed meal and its effect on performance, egg quality traits, and blood chemistry. Journal of Applied Poultry Research, 17(2), 229-236. https://doi.org/10.3382/japr.2007-00064.
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