اثر تغذیه سیلاژ یونجه با مقادیر مختلف خرمای ضایعاتی بر جمعیت پروتوزوآی شکمبه، میزان تولید پروتئین میکروبی و فراسنجه های خون در گوسفند کرمانی

نوع مقاله : علمی پژوهشی - تغذیه نشخوارکنندگان

نویسندگان

1 شهید باهنر کرمان

2 بخش علوم دامی، دانشگاه شهید باهنر کرمان، کرمان، ایران

چکیده

در این تحقیق، اثر تغذیه سیلاژ یونجه با مقادیر مختلف خرمای ضایعاتی بر سنتز پروتئین میکروبی، جمعیت پروتوزوآ و فرأسنجه های خونی در هشت رأس گوسفند نَر کرمانی با میانگین وزنی 2±47‌کیلوگرم در قالب طرح چرخشی در 2‌دوره 21‌روزه بررسی گردید. برای تهیه سیلاژ، یونجه با درصد های صفر، پنج، 10 و 15‌درصد خرمای ضایعاتی با هم مخلوط و به‌مدت 45‌روز در سطل هایی با گنجایش 40 لیتر سیلوگردید. پس از تعیین ترکیبات شیمیایی سیلاژ های یونجه ، از سیلاژها به‌میزان 30‌درصد در جیره های آزمایشی شامل: 1) جیره شاهد (سیلاژ یونجه بدون خرمای ضایعاتی)، 2) جیره حاوی سیلاژ یونجه با 5‌درصد خرمای ضایعاتی، 3) جیره حاوی سیلاژ یونجه با 10‌درصد خرمای ضایعاتی و 4) جیره حاوی سیلاژ یونجه با 15‌درصد خرمای ضایعاتی استفاده شدند. نتایج این تحقیق نشان‌داد که جمعیت کل پروتوزوآ و تمامی گونه های هولوتریش، سلولیتیک و انتودینیوم در مایع شکمبه با افزودن خرمای ضایعاتی به سیلاژ یونجه به‌صورت روند خطی افزایش یافت. هم‌چنین جیره حاوی سیلاژ یونجه با 15‌درصد خرمای ضایعاتی سبب افزایش ابقا نیتروژن و پروتئین میکروبی شد. با افزایش مقدار خرمای ضایعاتی در سیلاژ یونجه در جیره های آزمایشی، سطح کلسترول و نیتروژن اوره ای خون به‌صورت معنی داری تغییر پیدا کرد و سطح تری گلیسیرید خون به‌صورت روند خطی تحت تاثیر جیره های آزمایشی قرار گرفت. به‌طور کلی با توجه به نتایج به‌دست آمده، استفاده از مقدار 15‌درصد خرمای ضایعاتی در تهیه سیلاژ یونجه سبب بهبود ابقاء نیتروژن و افزایش سنتز پروتئین میکروبی شد. بنابراین می توان از این سیلاژ، در تغذیه دام استفاده نمود.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Feeding Ensiled Alfalfa with Different Levels of Waste Date on Rumen Protozoal Population, Microbial Protein Synthesis and Blood Parameters in Kermani Sheep

نویسندگان [English]

  • raheleh rajabi Aliabadi 1
  • Reza Tahmasbi 2
  • Omid Dayani 2
  • Amin Khezri 2
1 Shahid Bahonar University of Kerman
2 Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
چکیده [English]

Introduction Ensiling is less dependent to weather condition and farmers are trying to ensile forages for preserving them for livestock feeding. Ensiling legumes because of their buffering capacity and high moisture content are difficult. Also, ensiling alfalfa will lose up to %85 of its nitrogen content as non-protein nitrogen. Adding some additives like carbohydrate sources will improve alfalfa silage quality and its physical characteristics. There are some agricultural byproducts which can be used in animal feeding. Waste date is being produced annually in Iran and it can be used as carbohydrate source during alfalfa ensiling. This experiment was conducted to investigate ensiling alfalfa with different levels of waste date and its feeding effect on rumen protozoa population, microbial protein synthesis and blood parameters in Kermani sheep.
Materials and method Eight rams (47 ± 2 kg BW) were used in a 2×2 change over design experiment. Each experimental period was conducted for 21 days (16 days for adaptation and days for sampling). For ensiling, fresh alfalfa with different levels of waste date (0, 5, 10 and %15) were mixed together and ensiled in 100 L containers for 45 days. After opening chemical compositions of silages such as dry matter, crude protein, ammonia nitrogen, crude fat, organic matter, ash, NDF, ADF and pH were determined according to standard methods. Then, it was used %30% of diet in experimental diets as: 1) control diet (alfalfa silage without waste date); 2) alfalfa silage with 5% waste date; 3) alfalfa silage with 10% waste date and 4) alfalfa silage with 15% waste date. Treatment diets were mixed and fed as a TMR at 0800 and 1700 h. Amount of the TMR offered was recorded, and treatment diets were sampled daily for the last 5 d of each period. Orts were weighed, recorded, and sampled according to the same procedures followed for the treatment diets. Urine samples were acidified during collection to a pH

کلیدواژه‌ها [English]

  • Alfalfa silage
  • Blood parameters
  • Microbial protein synthesis
  • Waste date
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