مقایسه اثرات گل میمونی با سین بیوتیک و آنتی بیوتیک بر عملکرد و پاسخ ایمنی جوجه های گوشتی

نوع مقاله : علمی پژوهشی- تغذیه طیور

نویسندگان

1 دانشگاه ایلام

2 دانشگاه اراک

چکیده

این آزمایش به منظور مقایسه اثر افزودن گل میمونی با آنتی بیوتیک ویرجینامایسین و سین بیوتیک به جیره بر عملکرد و پاسخ ایمنی جوجه های گوشتی انجام شد. تعداد 250 قطعه جوجه خروس (سویه تجاری راس 308) یکروزه به 25 گروه 10 قطعه ای (پنج تیمار و پنج تکرار به ازای هر تیمار) تقسیم شدند. تیمارهای آزمایشی شامل جیره پایه بدون ماده افزودنی (جیره شاهد) و جیره پایه حاوی 02/0 درصد آنتی بیوتیک ویرجینامایسین، سطح تجاری سین بیوتیک (در جیره آغازین 15/0 درصد و در جیره های رشد و پایانی 10/0 درصد)، 4/0 و 8/0 درصد گیاه گل میمونی بود. نتایج حاصل از این آزمایش نشان داد که جوجه های تغذیه شده با جیره های حاوی گل میمونی، سین بیوتیک و آنتی بیوتیک در پایان دوره پرورش به طور معنی داری ضریب تبدیل بهتری نسبت به گروه شاهد داشتند. در مقابل، افزایش وزن و خوراک مصرفی جوجه ها در کل دوره (1 تا 42 روزگی) تحت تأثیر تیمارهای آزمایشی قرار نگرفت. اعمال همه تیمارهای آزمایشی به استثنای تیمار 4/0 درصد گل میمونی سبب افزایش معنی دار درصد لنفوسیت و کاهش معنی دار نسبت هتروفیل به لنفوسیت در مقایسه با تیمار شاهد شدند. پرنده های تغذیه شده با تیمارهای سین بیوتیک و 8/0 درصد گل میمونی به طور معنی دار پاسخ ثانویه پادتن بالاتری علیه واکسن های نیوکاسل و گامبورو را در مقایسه با پرندگان گروه شاهد نشان دادند. از نتایج این مطالعه می توان نتیجه گرفت که افزودن 8/0 درصد گل میمونی به جیره جوجه های گوشتی سبب بهبود بازده خوراک و پاسخ ایمنی می شود و می تواند بعنوان یک جایگزین مناسب برای آنتی بیوتیک در تغذیه جوجه های گوشتی مدنظر قرار گیرد.

کلیدواژه‌ها


عنوان مقاله [English]

Comparative effects of Scrophularia striata with synbiotic and antibiotic on performance and immune response of broiler chickens

نویسندگان [English]

  • Farhad Rostami 1
  • Kamran Taherpour 1
  • Hossein Ali Ghasemi 2
  • Fazel Purahmad 1
1 Ilam University
2 Arak University
چکیده [English]

Introduction: Following the ban on antimicrobial growth promoters in poultry nutrition in EU and growing pressure on poultry producers in other parts of the world, there is an increasing interest in searching for growth promoting and immune system-strengthening alternatives. Among the possible alternatives, probiotics, prebiotics, synbiotics (combination of probiotic and prebiotic) and more recently phytogenic products are considered interesting because they have acquired more reliability and acceptability among consumers as safe and natural additives. Scrophularia striata (SS) is a plant which grows in the northeastern part of Iran and their immunomodulatory activities of some species of Scrophularia have also been reported by other investigators. Due to the lack of study for Scrophularia striata effects on broilers performance, and the importance of improving the immune status of broilers, this study was conducted to evaluate the effect of S. striata on male broilers growth performance, heterophil to lymphocyte ratio and immunity; and also to compare them with virginiamycin as a well-documented antibiotic growth-promoter.

Materials and methods: Two-hundred and fifty of one-day-old male (Ross 308) broiler chicks were classified into 25 groups. Each group included 10 chicks (five treatments and five replicates per treatment). The five experimental treatments were as follow: basal diet with no additives (control diet) and basal diet containing virginiamycin antibiotic, synbiotic, 0.4 or 0.8 % SS. Feed intake (FI) and body weight gain (BWG) were recorded in different periods and feed conversion ratio (FCR) was calculated. To study the effects of different treatments on blood leukocyte subpopulations, blood samples of two birds from each replicate were collected from the wing vein at the end of experiment. EDTA-containing blood samples were stained subsequently, 100 leukocytes per samples were counted by an optical microscope. The ratio of heterophil (H) to lymphocyte (L) was calculated. Vaccination was carried out according to the routine regional vaccination program and was based on optimal timing of the maternal antibody level. On 7th and 14th days after last vaccination, blood samples were collected from brachial veins, and the sera were used to determine the humoral immune response derived from vaccination against Newcastle disease and infectious bursal disease. Haemagglutination inhibition tests and enzyme-linked immunosorbent assays were used to determine antibody titers of the chickens against Newcastle disease and infectious bursal disease, respectively. The data were analyzed in a completely randomized design by ANOVA using the General Linear Model (GLM) procedure of SAS Institute.

Results and Discussion: The BWG of broilers receiving the antibiotic diet was higher (P

کلیدواژه‌ها [English]

  • Broiler
  • Heterophil:lymphocyte
  • Immune response
  • Performance
  • Scrophularia striata
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