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امیرحسین شفیعی مختار فتحی تیمور تنها

چکیده

این مطالعه به منظور بررسی اثر نانوذرات سلنیوم بر فراسنجه­های خونی و متابولیت­های زرده در مرغ­های تخمگذار انجام شد. این آزمایش با 160 قطعه مرغ تخمگذار هایلاین W36 در قالب طرح کاملا تصادفی با 4 تیمار، 4 تکرار و 10 پرنده در هر واحد آزمایشی صورت پذیرفت. جیره­های آزمایشی شامل: جیره شاهد و سطوح 2/0، 3/0 و 5/0 میلی­گرم در کیلوگرم نانوسلنیوم بود. فراسنجه­های درصد تولید تخم­مرغ، وزن تخم­مرغ، خوراک مصرفی و ضریب تبدیل خوراک نیز برای کل دوره آزمایشی محاسبه شد. متابولیت­های سرم خون مورد آزمایش شامل: تعیین میزان مالون دی آلدئید، کلسترول تام، تری گلیسرید تام، آسپارتات آمینوترنسفراز، آلانین ترنسفراز و آنتی اکسیدان تام بود. متابولیت­های زرده تخم مرغ مورد آزمایش شامل: تعیین میزان غلظت سلنیوم زرده و سطح مالون دی آلدئید چربی­های زرده بود. نتایج نشان داد اضافه نمودن نانوسلنیوم سبب افزایش درصد تولید تخم­مرغ و بهبود ضریب تبدیل خوراک شد. سطوح مختلف نانوسلنیوم تاثیر معنی­داری بر میزان مالون­دی­آلدئید، کلسترول، تری­گلیسرید، فعالیت آنزیم­های آسپارتات آمینوترنسفراز و آلانین آمینوترنسفراز سرمی نداشتند. سطح 5/0 میلی­گرم در کیلوگرم نانوسلنیوم سبب بیشترین میزان سلنیوم در زرده نشان داد. ظرفیت آنتی اکسیدانی سرم به طور منحنی با افزایش سطح نانوسلنیوم تا 3/0 میلی گرم افزایش ولی در سطح 5/0 میلی کاهش یافت. میزان پروکسیداسیون چربی­های زرده تحت تأثیر مدت نگهداری و تیمارهای آزمایشی قرار گرفت به طوری که سطح 2/0 و 3/0 میلی گرم نانوسلنیوم سبب کاهش معنی­دار مالون دی آلدئید تخم مرغ در روز اول و 15 روز بعد از تخمگذاری شد.

جزئیات مقاله

کلمات کلیدی

پایداری اکسیداتیو تخم مرغ, عملکرد، فراسنجه خونی, مرغ تخمگذار, نانوسلنیوم, وضعیت آنتی¬اکسیدانی

مراجع
1. Arai, T., M. Sugawara, T. Sako, S. Motoyoshi. T. Shimura, N. Ttsutsui, and T. Konno. 1994. Glutathione peroxidase activity in tissues of chickens supplemented with dietary selenium. Comparative Biochemistry and Physiology, 107: 245-248.
2. Balogh, K., M. Weber, M. Erdelyi, and M. Mezes. 2004. Effect of excess selenium supplementation on the glutathione redox system in broiler chicken. Acta Veterinaria Hungarica, 52:403–411.
3. Benzie, I. F., and J. J. Strain. 1996. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Analytical Biochemistry, 239:70-76.
4. Boostani, A., A.A. Sadeghi, S. N. Mousavi, M. Chamani, and N. Kashan. 2015. The effects of organic, inorganic, and nano-selenium on blood attributes in broiler chickens exposed to oxidative stress. Acta Scientiae Veterinariae, 43: 1-6.
5. Cai, S., C. Wu, L.M. Gong, Y. Song, H. Wu, and L. Y. Zhang. 2012. Effects of Nano-selenium on performance, meat quality, immune function, oxidation resistance and tissue selenium content in broilers. Poultry Science, 91(10): 2532-9.
6. Cantor, A. H., M. L. Straw, M. J. Ford, A. J. Pescatore, and M. K. Dunlap. 2000. Effect of feeding organic selenium in diets of laying hens on egg selenium content. Page 473 in Egg Nutrition and Biotechnology. J. S. Sim, S. Nakai, and W. Guenter, ed. CABI Publishing, New York, NY.
7. Chamsaz, M., M. H. Arbabzavar, and M. M. Riazi. 2007. Determination of selenium(IV) by Electrothermal Atomic Absorption Spectrometry using Single Drop Extraction in Real Samples. Asian Journal of chemistry,19:3475-82.
8. Christine, A., R. Zuberbuehler, E. Messikommer, M. Myrtha, Arnold, Rhea S. Forrer, and W. Casper. 2006. Effect of selenium depletion and selenium repletion by choice feeding on selenium status of young and old laying hens. Physiology & Behavior, 87: 430-40.
9. Christinsen, M. J. and Burgener, K.W. 1992. Dietary selenium stabilizes glutathione peroxidase mRNA in rat liver. Journal of Nutrition, 122: 1620-1626.
10. Čobanová, K., V. Petrovič, M. Mellen, H. Arpášova, L. Grešáková, and S. Faix. 2011. Effects of dietary form of selenium on its distribution in eggs. Biological trace element research, 144(1-3), 736-746.
11. Davis, R. H. and J. Fear. 1996a. incorporation of selenium into egg proteins from dietary selenite. Br Poultry Science, 37(1):197-211.
12. Davis, R. H., J. Fear, and A. C. Winton. 1996b. Interactions between dietary selenium, copper, and sodium nitroprusside, a source of cyanide in growing chicks and laying hens. British Poultry Science, 37:87-94.
13. Del Rio, D., A. J. Stewart, and N. Pellegrini. 2005. A review of re- cent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr. Met. Card. Dis. 15:316–328.
14. Desai, M. P., V. Labhasetwar, E. Walter, R. J. Levy, and G. L. Amidon. 1997. The mechanism of uptake of biodegradable microparticles in Caco-2 cells is size dependent. Pharmaceutical Research, 14(11):1568-1573.
15. Farrel, D.J. 2002. Adding Value to the Hen`s Egg. School of Land and Food Science. The University of Queensland, Qld 4072. Australia.
16. Fathi, M., M. Haydari, and T. Tanha. 2015. Effects of Enalapril on Performance growth, Ascites Mortality, Antioxidant Status and Blood Parameters in Broiler Chickens Under Cold-Induced Ascites. Poultry Science Journal, 3 (2): 121-127.
17. Fathi, M., M. Haydari, and T. Tanha. 2016. Influence of Dietary Aspirin on Growth Performance, Antioxidant Status, and Mortality due to Ascites in Broiler Chickens. Poultry Science Journal. Poultry Science Journal, 4 (2): 139-146
18. Habibi, R., H. Delivery, R. Spring, M. Taghizadeh, S. Soleimani, And M. Ali Hasan Maslak. 2014. Comparison of the effects of three forms of selenium (mineral, organic and nano) on the activity of enzyme glutathione peroxidase and serum metabolites in broiler chicks. Sixth Conference of Agricultural Research Findings. The University of Kordestan. P. 33-30. (In Persian)
19. Henry, P. R, and C. B. Ammerman. Selenium bioavailability, 1995. Page 301 in Bioavailability of Nutrients for Animals: Amino acids, minerals, and vitamins.
20. Jiang, Z.Y., Y. C. Lin, G. L. Zhou, L. H. Luo, S.Q. Jiang, and F. Chen. 2009. Effects of dietary selenomethionine supplementation on growth performance, meat quality and antioxidant property in yellow broilers. Journal Agricalter Food, 57: 9769-9772.
21. Jing, C. L, X. F. Dong, Z. M. Wang, S. Liu, and J. M. Tong. 2015. Comparative study of DL-selenomethionine vs sodium. selenite and selenoyeast on antioxidant activity and selenium status in laying hens. Poultry Science, 94: 965-975.
22. Mikulski, D., J. Jankowski, Z. Zdunczyk, M. Wroblewska, K. Sartowska, and T. Modjeska. 2009. The effect of selenium source on performance, carcass traits, oxidative status of the organism and meat quality of turkeys. Journal of Animal and Feed Sciences, 518–530.
23. Mohamadi, R., F. Farokhi, V. Najati, and R. S. Airs. 2012. Assessment of the amount of hepat ohistopatological and enzymatic changes after chronic lead intoxication in utero and throughout life in rat. Qom Univercity Medicine Science Journal, 7(1):83-90.
24. Mohiti-Asli, M., F. Shariatmadari, H, Lotfollahian, and M. T. Mazuji. 2008. Effects of supplementing layer hen diets with selenium and vitamin E on egg quality, lipid oxidation and fatty acid composition during storage. Canadian journal of animal science, 88(3): 475-483.
25. Nassir, F., C. Moundras, D. Bayle, C. Serougne, E. Gueux, E. Rock, Y. Rayssiguier, and A. Mazur. 1997. Effect of selenium deficiency on hepatic lipid and lipoprotein metabolism in the rat. British Journal of Nutrition, 78:493–500.
26. Nielsen, F., B. B. Mikkelsen, J. B. Nielsen, H. R. Andersen, and P. Grandjean. 1997. Plasma malondialdehyde as biomarker for oxidative stress: Reference interval and effects of life-style factors. Clinical Chemestry, 43:1209–1214.
27. Payne, R. L. and L. L. Southern. 2005. Changes in glutathione peroxidase and tissue selenium concentrations of broilers after consuming a diet adequate in selenium. Journal of poultry science, 84: 1268-1276.
28. Petrovic, V., K. Boldizarova, S. Faix, M. Mellen, H. Arpasova and L. Leng. 2006. Antioxidant and selenium status of laying hens fed with diets supplemented with selenite or Se-yeast. Journalof Animal and Feed Science, 15: 435-444.
29. Ramezani, S., A. Riazi, N. Afzali, And M. H. Fathi Nasiri. 2013. The effect of selenium and sodium bicarbonate on blood biochemical traits, performance and carcass characteristics of broiler chicks under thermal stress conditions. Veterinary Journal (Research and Development), No. 9 (In Persian).
30. Ryu, Y. C., M. C. Rhee, K. M. Lee, and B. C. Kim. 2005. Effect of different levels of dietary supplemental selenium on performance, lipid oxidation, and color stability of broiler chiks. Journal of poultry Science, 84:809-15.
31. Scheideler, S., P. Weber, and D. Monsalve D. 2010. Supplemental vitamin E and selenium effects on egg production, egg quality, and egg deposition of α-tocopherol and selenium. Journal of Applied Poultry Research, 19(4): 354-360.
32. Shen, H., C. Yang, J. Liu, and C. Ong. 2000. Dual role of glutathione in seleniteinduced oxidative stress and apoptosis in human hepatoma cells. Free Radical in Biology and Medicine, 28: 1115-1124.
33. Skrivan, M., I. Bubancová, M. Marounek, and G. Dlouhá. 2010. Selenium and alpha-tocopherol content in eggs produced by hens that were fed diets supplemented with selenomethionine, sodium selenite and vitamin E. Czech Journal of Animal Science-UZEI (Czech Republic).
34. Strougne, C., C. Felgines, J. Ftrtzou, T. Hajri, C. Bertin, and A. Mazur. 1995. Hypercholesterolemia induced by cholesterol- or cystine-enriched diets is characterized by different plasma lipoprotein and apolipoprotein concentrations in rats. Journal of Nutrition, 125, 35-41.
35. Surai. P. 2002. Selenium in poultry nutrition 1. Antioxidant properties, deficiency and toxicity. World's Poultry Science Journal, 58(3), 333-347.
36. Torres. S.K, V. L. Campos, and C. G. Leon. 2012. Biosynthesis of selenium nanoparticles by Pantoea aggl. omerans and their antioxidant activity. Journal of Nanoparticle Research, 14(11):1236.
37. Utterbck, P.L., C. M. Parsons, I. Yoon, and J. Butlert. 2005. Effct of supplementing selenium yeast in diets of laying hens on egg selenium content. Poultry Science, 84:1900-1901.
38. Lawyer, R. and M. Bahram. 2010. Effect of Different Levels of Selenium on Metabolic Blood Metabolic and Hemorrhagic Safety in Broiler Chickens. Journal of Veterinary Research, (4) 65: 336-329. (In Persian).
39. Wang, H., J. Zhang, and H. Yu. 2007. Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: comparison with selenomethionine in mice. Free Radical Biology Medecine, 42(10):1524-1533.
40. Weiss, S. L., J. K. Evenson, K. M. Thompson, and R. A. Sunde. 1997. Dietary selenium regulation of glutathione peroxidase mRNA and other eleniumdependent parameters in male rats. Journal of Nutrition Biochemestry, 8:85.
41. Xia, M., H. Zhang, C. Hu and Z. Xu. 2005. Effect of nanoselenium on growth performance and antioxidant function of broiler chicken. Nutrimenta Sinica, 27:307-310.
42. Zainli, A., A. Riazi, and H. Kermanshahi. 2009. Effect of Sodium Selenite and Turmeric Powder on Performance, Carcass Quality and Antioxidant Metabolites of Broiler Chickens under Thermal Stress Conditions. Journal of Animal Science Research, (2) 1: 70-85. (In Persian).
43. Zhang, J., X. Wang, and T. Xu. 2008. Elemental selenium at nano size (Nano-Se) as a potential chemopreventive agent with reduced risk of selenium toxicity: comparison with Se-methylselenocysteine in mice. Toxicology Science,101(1):22-31.
ارجاع به مقاله
شفیعیا., فتحیم., & تنهات. (2020). بررسی تأثیر نانوذرات سلنیوم بر عملکرد، وضعیت آنتی اکسیدانی، فراسنجه‌های خونی و پایداری اکسیداتیو تخم مرغ در مرغان تخم¬گذار. پژوهشهای علوم دامی ایران, 12(3), 323-335. https://doi.org/10.22067/ijasr.v12i3.79464
نوع مقاله
علمی پژوهشی- تغذیه طیور