تأثیر سلنیوم به همراه ویتامین E بر عملکرد تولیدی، پاسخ ایمنی و متابولیت‌های خونی بلدرچین‌ ژاپنی تخم‌گذار

نوع مقاله : علمی پژوهشی- تغذیه طیور

نویسندگان

1 گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی اهر، دانشگاه تبریز، تبریز، ایران

2 گروه علوم دامی،دانشکده کشاورزی و منابع طبیعی اهر، دانشگاه تبریز، تبریز، ایران.

چکیده

این آزمایش به منظور بررسی اثرات توأم منابع مختلف سلنیوم به همراه ویتامین E بر عملکرد، خصوصیات کیفی، سیستم ایمنی و متابولیت­های خونی بلدرچین­ ژاپنی تخم‌گذار انجام شد. تعداد 144 قطعه پرنده بلدرچین ماده در قالب طرح کاملاً تصادفی در بین 3 تیمار با 4 تکرار و 12 قطعه در هر تکرار توزیع شد. تیمارهای آزمایشی شامل جیره پایه (بدون افزودنی) و دو منبع سلنیوم آلی (سلپلکس) و معدنی (سلنیت سدیم) همراه با 120 میلی­گرم ویتامین E­ در کیلوگرم جیره بود که هر کدام از منابع به میزان 4/0 میلی­گرم در کیلوگرم جیره غذایی سلنیوم تامین کرد. نتایج نشان­داد میانگین وزن­تخم، درصد تخم­گذاری و ضریب تبدیل­غذایی در مقایسه با گروه کنترل تحت تأثیر منابع آلی و معدنی سلنیوم قرار نگرفت. پرندگان دریافت­کننده جیره­غذایی حاوی سلنیوم­آلی به همراه ویتامین E در مقایسه با سایر تیمارها مصرف خوراک پایین­تر داشتند و ضریب تبدیل غذایی آن‌ها از نظر عددی کاهش و تمایل به معنی داری داشت. سلنیوم­آلی به همراه ویتامین E در جیره­غذایی سبب بهبود ارتفاع­سفیده، واحد هاو، pH زرده و شاخص­زرده در مقایسه با گروه شاهد شد. صفات­ کیفی تخم شامل شاخص­زرده و سطح­پوسته در گروه سلنیوم­معدنی در مقایسه با گروه کنترل افزایش معنی­دار نشان­داد. غلظت سلنیوم­زرده در گروه های سلنیوم­آلی و معدنی به همراه ویتامین E نسبت به گروه شاهد افزایش نشان ­شد که از لحاظ تغذیه­ای بهتر است. میزان کلسترول­کل و تری­گلیسیریدخون تحت تأثیر تیمار­های­آزمایشی قرارنگرفت. میزان مالونیل­دی­آلدهید در زرده تخم­های ذخیره­شده در گروه شاهد بیش‌ترین و در گروه حاوی سلنیوم­آلی کمترین مقدار بود. می­توان نتیجه گرفت افزودن سلنیوم آلی در مقایسه با سلنیوم­معدنی در افزایش سلنیوم زرده و حفظ کیفت تخم بلدرچین در طول دوره ذخیره­سازی موثرتر است.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Selenium with Vitamin E on Production Performance, Immune Response and Blood Metabolites of Laying Japanese Quail

نویسندگان [English]

  • Hossain Ahmadian 1
  • zabihollah Nemati 1
  • Amir Karimi 2
  • Rashid Safari 1
  • Mohammad Reza Sheeikhlou 1
  • Maghsood Besharati 1
1 Department of animal science, Faculty of Agriculture and Natural Resources, University of Tabriz, I.R. Iran
2 Department of Animal Science, Ahar Faculty of Agriculture and Natural Resources, University of Tabriz, Tabriz, Iran
چکیده [English]

Introduction: Selenium is an essential trace element, which plays a key role in the development of reproductive performance and animal safety. This mineral is an important part of at least 25 effective proteins in a variety of physiological functions, including increased resistance to oxidative stress, DNA repair, and improvement to reproductive performance and immune function. Despite the fact that selenium requirement of birds can be met by corn-soybean meal diet, dietary sodium selenite up to a maximum level of 0.5 mg kg-1 is highly recommended to improve avian health and productive performance. However, interaction with other minerals, poor retention and lower deposition efficiency questioning the incorporation of sodium selenite into the diet. Vitamin E is the main antioxidant in egg yolk lipid terminating the lipid peroxidation chain by reacting with lipid peroxides and making them be stable. Diet supplementation with vitamin E could improve performance and egg quality and provided health benefits to laying hens. The positive effects of Se and vitamin E on egg production, egg quality traits has been extensively studied in laying hens, but information regarding the potential benefits of these antioxidants on the productive performance of Japanese quails is limited in literature. Thus, this study was conducted to investigate the combined effects of various sources of selenium (0.4 mg kg-1) plus vitamin E (120 mg kg-1) on performance, qualitative characteristics, immune system and blood metabolites of Japanese quails.
Materials and Methods: A total of 144 12-week-old female Japanese quails were randomly divided into three treatment groups with four replicates and twelve birds in each in a completely randomized design. The experimental treatments consisted of basal diet (with no supplementation) and two organic (sel-plex) and inorganic (sodium selenite) sources of selenium which provided 0.4 mg/kg, plus 120 mg of vitamin E per kilogram of diet. Feed intake, feed conversion ratio (FCR), egg production and quality parameters in fresh and stored eggs, cell immunity, blood cholesterol, triglyceride and total antioxidant contents were evaluated. At the end of experiment, egg yolk selenium and malondialdehyde concentrations were measured. The thiobarbituric acid reactive substances (TBARS) assay with small modification was used to measure lipid oxidation. Briefly, 2 grams of homogenous yolk was mixed with 5 mL of 20 % trichloroacetic acid (TCA) and 4 mL distilled water, and homogenized for 30 s at high speed. The homogenate was then centrifuged (1000 g for 20 min) and the resulting supernatant was filtered with Whatman Filter Paper (grade no. 1). Two mL of filtrated was mixed with 2 mL of thiobarbituric acid (TBA, 0.02 M) in a test tube and heated in boiling water for 20 min. After cooling, the absorbance of the resulting solution was read with a spectrophotometer at 532 nm.
Results and discussion: The results showed that egg weight, egg laying percentage and feed conversion rate were not affected by selenium organic and inorganic sources compared to control group (P > 0.05). Birds receiving organic selenium along with vitamin E in their diets had low feed intake compared to other treatments and their feed conversion ratio numerically decreased and intended to significant (p<0.1).The organic selenium plus vitamin E improved the height of white, HU units, yolk pH, and yolk index compared to control group. Egg quality parameters including yolk index and shell surf index in inorganic selenium group were significantly higher than control group (p<0.0.05). Egg yolk selenium was increased in organic and organic selenium (2.06, 1.31 mg/kg) compared to control (0.36), which is a better value from the nutritional viewpoint. Blood cholesterol and triglyceride levels were not affected by experimental diets. Concentration of yolk MDA was highest in control and lowest in organic selenium group during storage (P < 0.05). The result of this study showed that egg production, feed conversion ratio and total egg mass were not affected by experimental treatments. This was also observed by other researchers who evaluated the dietary inclusion of vitamin E. In another one egg quality parameters, however, egg weight was significantly raised after being fed with a diet contained 120 mg kg-1 of vitamin E and 0.4 mg kg-1 of organic selenium.
Conclusion: In conclusion, our study data revealed dietary organic selenium and vitamin E, could improve the egg quality during storage and increased egg yolk selenium of Japanese quails.
 

 
 

کلیدواژه‌ها [English]

  • Blood metabolites
  • egg quality
  • Japanese quail
  • sel-plex
  • sodium selenite
  1. Abbaszadeh Mobaraki, M., and H. Aghdam Shahryar. 2015. The impact of different levels of vitamin E and selenium on the performance, quality and the hatchability of eggs from breeding Japanese quails. Iranian Journal of Applied Animal Science, 5: 927-932.
  2. Ahmadian, H., Z. Nemati, A. Karimi, and R. Safari. 2019. Effect of different dietary selenium sources and storage temperature on enhancing the shelf life of quail eggs. Animal Production Research, 8: 23–33. (In Persian).
  3. Ahmadi, F., and F. Rahimi. 2011. Factors affecting quality and quantity of egg production in laying hens: a review. World Applied Sciences Journal, 12: 372-384.
  4. Arpasova, H., J. Weis, P. Hascik, and M. Kacaniova. 2009. The effects of sodium selenite and selenized yeast supplementation into diet for laying hens on selected qualitative parameters of table eggs. Scientific Papers Animal Science and Biotechnologies, 42: 408-414.
  5. Arthur, J. 1992. Selenium metabolism and function. Proceedings of the Nutrition Society of Australia. 17: 91-8.
  6. Arthur, J. R., R. C. McKenzie, and G. J. Beckett. 2003. Selenium in the immune system. The Journal of Nutrition, 133: 1457S-1459S.
  7. Attia, Y., A. Abdalah, H. Zeweil, F. Bovera, A. T. El-Din, and M. Araft. 2010. Effect of inorganic or organic selenium supplementation on productive performance, egg quality and some physiological traits of dual-purpose breeding hens. Czech Journal of Animal Science, 55: 505-519.
  8. Bennett, D. C., and K. Cheng. 2010. Selenium enrichment of table eggs. Poultry Science, 89: 2166-2172.
  9. Biswas, A., M. Ahmed, V. Bharti, and S. Singh. 2010. Effect of antioxidants on physiobiochemical and hematological parameters in broiler chicken at high altitude. Asian Australasian Journal of Animal Sciences, 24: 246-249.
  10. Biswas, A., J. Mohan, and K. Sastry. 2006. Effect of higher levels of dietary selenium on production performance and immune responses in growing Japanese quail. British Poultry Science, 47: 511-515.
  11. Cantor, A., M. Straw, M. Ford, A. Pescatore, and M. Dunlap. 2000. Effect of feeding organic selenium in diets of laying hens on egg selenium content. Egg Nutrition and Biotechnology. JS Sim, S. Nakai, and W. Guenter, ed. CABI Publishing, New York, NY, 473.
  12. Chitra, P., S. Edwin, and M. Moorthy. 2013. Dietary inclusion of vitamin E and selenium on egg production, egg quality and economics of Japanese quail layers. Turkish Journal of Veterinary and Animal Sciences, 9: 51-60.
  13. Choct, M., A. Naylor, and N. Reinke. 2004. Selenium supplementation affects broiler growth performance, meat yield and feather coverage. British Poultry Science, 45: 677-683.
  14. Council, N. R. 1994. Nutrient requirements of poultry. Washington, DC, National Academic Press, 42: 57-63.
  15. Faustman, C., S. Specht, L. Malkus, and D. Kinsman. 1992. Pigment oxidation in ground veal: Influence of lipid oxidation, iron and zinc. Meat Science, 31: 351-362.
  16. Gajcevic, Z., G.Kralik, E. Has-Schon, and V. Pavic. 2009. Effects of organic selenium supplemented to layer diet on table egg freshness and selenium content. Italian Journal of Animal Science, 8: 189-199.
  17. Galobart, J., A.Barroeta, M. Baucells, R. Codony, and W. Ternes. 2001. Effect of dietary supplementation with rosemary extract and α-tocopheryl acetate on lipid oxidation in eggs enriched with ω3-fatty acids. Poultry Science, 80: 460-467.
  18. Gebert, S., R. Messikommer, H. Pfirter, G. Bee, and C. Wenk. 1998. Dietary fats and vitamin E in diets for laying hens: Effects on laying performance, storage stability and fatty acid composition of eggs. Archiv fuer Gefluegelkunde.
  19. Haugh, R. 1937. The Haugh unit for measuring egg quality. United States Egg Poultry Magazine, 43: 522-555.
  20. Heindl, J., Z. Ledvinka, E. Tumova, and L. Zita. 2010. The importance, utilization and sources of selenium for poultry: a review. Scientia Agriculturae Bohemica, 41: 55-64.
  21. Hidiroglou, N., G. S. Gilani, L. Long, X. Zhao, R. Madere, K. Cockell, B. Belonge, W. N. Ratnayake, and R. Peace. 2004. The influence of dietary vitamin E, fat, and methionine on blood cholesterol profile, homocysteine levels, and oxidizability of low density lipoprotein in the gerbil. The Journal of Nutritional Biochemistry, 15: 730-740.
  22. Idowu, O., T. Laniyan, O. Kuye, V. Oladele Ojo, and D. Eruvbetine. 2006. Effect of copper salts on performance, cholesterol, residues in liver, eggs and excreta of laying hens. Archivos de Zootecnia, 55: 327-338.
  23. Kirunda, D., and S. Scheideler. The efficacy of vitamin E (DL-α-tocopheryl acetate) supplementation in hen diets to alleviate egg quality deterioration associated with high temperature exposure. Poultry Science, 80: 1378-1383.
  24. Kralik, G., Z. Gajcevic, P. Suchy, E. Strakova, and D. Hanzek. 2009. Effects of dietary selenium source and storage on internal quality of eggs. Acta Veterinaria Brno, 78: 219-222.
  25. Lin, YF., H. L. Tsai, Y.C. Lee, and S. J. Chang. 2005. Maternal vitamin E supplementation affects the antioxidant capability and oxidative status of hatching chicks. The Journal of Nutrition, 135: 2457-2461.
  26. Marsh, J. A., R. R. Dietert, and G. F. Combs Jr. 1981. Influence of dietary selenium and vitamin E on the humoral immune response of the chick. Proceedings of the Society for Experimental Biology and Medicine, 166: 228-236.
  27. Martin, L. B., P. Han, J. Lewittes, J. R. Kuhlman, K. C. Klasing, and M. Wikelski. 2006. Phytohemagglutinin induced skin swelling in birds: histological support for a classic immunoecological technique. Functional Ecology, 20: 290-299.
  28. Nemati, Z., K. Alirezalu, M. Besharati, S. Amirdahri, D. Franco, and J.M. Lorenzo. 2020. Improving the quality characteristics and shelf life of meat and growth performance in goose fed diets supplemented with vitamin E. Foods, 9 (6): 798.
  29. Nemati, Z., H. Ahmadian, M. Besharati, S. Lesson, K. Alirezalu, R. Domínguez, and J.M. Lorenzo. 2020. Assessment of dietary selenium and vitamin E on laying performance and quality parameters of fresh and stored eggs in Japanese quails. Foods, 9 (9): 1324
  30. Pappas, A. C., F. Karadas, P.F. Surai, and B.K. Speake. 2005. The selenium intake of the female chicken influences the selenium status of her progeny. Comparative Biochemistry Physiology Part B: Biochemistry Molecular Biology, 142: 465-474.
  31. Payne, R., T. Lavergne, and L. Southern. 2005. Effect of inorganic versus organic selenium on hen production and egg selenium concentration. Poultry Science, 84: 232-237.
  32. Puthpongsiriporn, U., S. Scheideler, J. Sell, and M. Beck. 2001. Effects of vitamin E and C supplementation on performance, in vitro lymphocyte proliferation, and antioxidant status of laying hens during heat stress. Poultry Science, 80: 1190-1200.
  33. Qi, G. H., and J. Sim. 1998. Natural tocopherol enrichment and its effect in ω3-fatty acids modified chicken eggs. Journal of Agricultural and Food Chemistry, 46: 1920-1926.
  34. Qu, X., K. Huang, L. Deng, and H. Xu. 2000. Selenium deficiency induced alterations in the vascular system of the rat. Biological Trace Element Research, 75: 119-128.
  35. Qureshi, M., J. Petitte, S. Laster, and R. Dietert. 1993. Avian macrophages: contribution to cellular microenvironment and changes in effector functions following activation. Poultry Science, 72: 1280-1284.
  36. Rengaraj, D., and Y. Hong. 2015. Effects of dietary vitamin E on fertility functions in poultry species. International Journal of Molecular Sciences, 16: 9910-9921.
  37. Sahin, N., K. Sahin, and M. Onderci. 2003. Vitamin E and selenium supplementation to alleviate cold stress associated deterioration in egg quality and egg yolk mineral concentrations of Japanese quails. Biological Trace Element Research, 96: 179-189.
  38. Sarıca, S., H. Aydın, and G. Ciftci. 2017. Effects of Dietary Supplementation of Some Antioxidants on Liver Antioxidant Status and Plasma Biochemistry Parameters of Heat-Stressed Quail. Turkish Journal of Agriculture Food Science and Technology, 5: 773-779.
  39. SAS Institute. 2009. SAS State Software. Changes and Enhancement Through Release, 8.2. SAS Institute, Inc., Cary, N.C.
  40. Scheideler, S., P. Weber, and D. Monsalve. Supplemental vitamin E and selenium effects on egg production, egg quality, and egg deposition of α-tocopherol and selenium. Journal of Applied Poultry Research, 19: 354-360.
  41. Serougne, C., C. Felgines, J. Férézou, T. Hajri, C. Bertin, and A. Mazur. 1995. Hypercholesterolemia induced by cholesterol or cystine enriched diets is characterized by different plasma lipoprotein and apolipoprotein concentrations in rats. The Journal of Nutrition, 125: 35-41.
  42. Shahriar, H. A., M. Shivazad, M. Chamani, K. Adl, and Y. Abrahiminejad. 2008. Effects of dietary fat type and different levels of vitamin E on performance and some of eggs characters of broiler breeder. Poultry Science, p 91-91.
  43. Skrivan, M., M. Marounek, G. Dlouha, and S. Sevcikova. 2008. Dietary selenium increases vitamin E contents of egg yolk and chicken meat. British Poultry Science, 49: 482-486.
  44. Surai, P. F. 2002a. Selenium in poultry nutrition Antioxidant properties, deficiency and toxicity. World's Poultry Science Journal, 58: 333-347.
  45. Surai, P. F. 2002b. Natural antioxidants in avian nutrition and reproduction. Nottingham University Press Nottingham.
  46. Urso, U., F. Dahlke, A. Maiorka, I. Bueno, A. Schneider, D. Surek, and C. Rocha. 2015. Vitamin E and selenium in broiler breeder diets: Effect on live performance, hatching process, and chick quality. Poultry Science, 94: 976-983.
  47. Utterback, P., C. Parsons, I. Yoon, and J. Butler. 2005. Effect of supplementing selenium yeast in diets of laying hens on egg selenium content. Poultry Science, 84: 1900-1901.
  48. Vakili, R., and M. Bahram. 2010. Effects of different dietary levels of selenium on metabolic parameters and humoral immunity in broiler chickens. Journal of Veterinary Research, 65: 329-336.
  49. Zadeh adamnejad, H., J. Ghiasi, and Y. Ebrahiminejad. 2015. Effect of different levels of selenium and vitamin E on blood biochemical parameters in the Japanese quail. Veterinary Clinical Pathology, 9: 243-252 (In Persian).

 

CAPTCHA Image