Genetic Diversity in Four Microsatellite Loci BMS1915, BMS1350, LGB and ILSTS45 in Baluchi Sheep

Document Type : Genetics & breeding

Authors

Reza Valizadeh ¹

Mohammadreza Nassiry ²

Ali Asghar Aslaminejad ²

Gholam Reza Dashab ³

Davoud Ali Saghi ⁴

M. Gholizadeh

¹ Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

² Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

³ Department of Animal Sciences, Faculty of Agriculture, University of Zabol, Zabol, Iran

⁴ Khorasan Agricultural and Natural Resources Research and Training Center

10.22067/ijasr.v5i1.24510

Abstract

The poly morphism of 4 micro satellite loci (BMS1350, LGB, ILSTS45, BMS1915) associated with functional traits were evaluated for detecting the diversity level in Baluchi breed of sheep. One hundred eighty five blood samples were collected from sheep herd in Abbas-Abad breeding station. DNA extraction was performed using guanidinethiocyanate silica gel method. Gene fragments of micro satellites were amplified by polymerase chain reaction based on the recommended standard method. The PCR products were visible using poly acry lamide gel electrophoresis and stain methods with silver. Alleles were typed using the Photocapt software version 3. All loci were in the Hardy-Weinberg equilibrium (p

Keywords

Polymorphism

Baluchi sheep

Microsatellite marker

Genetic diversity

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