Evaluation of Parentage Test in some of Holstein Cattle Using Fluorescent Labeled Microsatellite Markers

Document Type : Scientific - Research Articles

Authors

Mona Hashemi ¹

Sirouse Amirinia ²

Mohammad Taher Harkinezhad ¹

Hossein Banabazi ²

¹ Department of Animal Science, Zanjan Faculty of Agriculture, Zanjan, Iran

² Department of Biotechnology, Animal Science Research Institute of IRAN (ASRI), Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran

10.22067/ijasr.v4i2.15145

Abstract

In order to evaluate paternity test using microsatellite markers, a small sample of Iranian Holstein cattle population including 8 unknown individuals without any given prior knowledge on their genetic relationships were tested using 12 microsatellite loci recommended by International Society of Animal Genetics (ISAG). They were genotyped by a multiplex PCR set consisted all 12 fluorescently labeled primer pairs. Allele and genotype frequencies were estimated and simulated by CERVUS 2.0 software for paternity analysis. Using this software parentage test and relationship between samples have been determined and confirmed by sample’s provider. Paternity relationship including one or two parents for an offspring and also repeated samples were observed. The average expected heterozygosity of 11 analyzed loci and the mean value for PIC was 0.72 and 0.631 respectively. Except TGLA53 locus that was excluded from further analyses due to low quality of alleles and genotype detection, total exclusion probability of 11 loci showed that the paternity test using microsatellite loci mentioned here can be successfully implicated in large scale in Holstein dairy cattle population in Iran.

Keywords

Parentage test

Microsatellite marker

Holstein cattle

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