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ژیلا حسانی محمدرضا نصیری محمدرضا بختیاری زاده مجتبی طهمورث پور علی جوادمنش

چکیده

در این مطالعه با استفاده از داده¬های توالی¬یابی RNA-seq، به¬دست ¬آمده از 12 قطعه مرغِ ماده، بومی اصفهان و تجاری راس به بررسی ژن¬های با بیان متفاوت ،به شناسایی مهمترین ژن¬های مؤثر در سیستم آپوپتوزیز و تقویت آن پرداخته شد. در این بررسی کیفیت داده¬ها با استفاده از نرم¬افزار FastQC سنجیده شد. پس از حذف خوانش¬های بی کیفیت، با استفاده از نرم افزار Trimmomatic، نقشه یابی داده¬ها با استفاده از نرم¬افزار TOP HAT2 انجام گرفت. شبکة ژنی آپوپتوزیز توسط پایگاه استرینگ رسم و آنالیز شد. تحلیل شبکه نشاندهنده معنی¬داریِ شبکه بوده و میزان و چگونگی ارتباط ژن¬ها مشخص شد. نتایج حاصل از بررسی ترنسکریپتوم¬های سیستم ایمنی با استفاده از Cuffdif نشان داد که از بین 1328 ژن دارای تفاوت بیان معنی¬دار در سیستم ایمنی، 11 ژن مربوط به آپوپتوزیز بود. همچنین ژن PIK3CD دارای بیشترین مقدارِ بیان در دو نژاد بومی و تجاری و PIK3CB دارای کمترین مقدارِ بیان در نژاد بومی بودند. بیشترین معنی¬داری در فرایندهای بیولوژیکی بر اساس آماره Bonferroni، مربوط به مسیرِ علامت¬دهی وابسته به اینوزیتول و بر اساس آماره P-value مربوط به مسیرِ تنظیم موضعی¬کردن پروتئین به سمت هسته می¬باشد. آنالیز شبکه ژنیِ آپوپتوزیز، منجر به شناسایی دو زیر شبکه (کلاستر) گردید که ژن شاخص آنها به ترتیب PIK3R1 و IL1R1 می باشد.

جزئیات مقاله

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ارجاع به مقاله
حسانیژ., نصیریم., بختیاری زادهم., طهمورث پورم., & جوادمنشع. (2017). آنالیز بیانِ ژن‌ها و رسم شبکة ژنی آپوپتوزیس در نژادهای مرغ بومی اصفهان و تجاری راس تحت داده‌های RNA-seq. پژوهشهای علوم دامی ایران, 10(1), 117-130. https://doi.org/10.22067/ijasr.v10i1.66151
نوع مقاله
علمی پژوهشی- ژنتیک و اصلاح دام و طیور