اثر جاذب‌های آلی و معدنی آفلاتوکسین B1 بر قابلیت هضم و متغیرهای تخمیری شکمبه در شرایط برون‌تنی

نوع مقاله : علمی پژوهشی - تغذیه نشخوارکنندگان

نویسندگان

1 گروه علوم دامی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

2 علوم دامی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

مایکوتوکسین‌ها، متابولیت‌های تولید شده توسط قارچ‌ها هستند که باعث بروز مسمومیت در حیوانات و انسان می‌شوند. از مهم‌ترین مایکوتوکسین‌ها، آفلاتوکسین‌ها هستند که یکی از روش‌های مؤثر برای مقابله با مسمومیت ناشی از آنها، استفاده از جاذب‌های سموم قارچی می‌باشد. هدف از این مطالعه، مقایسه توانایی جاذب‌های مختلف بنتونیت تجاری، بنتونیت فعال، کربن فعال، مخمر و فرم‌های ترکیبی بنتونیت+کربن فعال+مخمر به میزان (1 درصد جیره)، در جذب آفلاتوکسینB1 در سطح 5/0 پی پی ام در شرایط برون‌تنی و تأثیر آنها بر قابلیت هضم و فرآسنجه‌های تخمیری شکمبه بود. افزودن جاذب‌های فوق به محیط کشت حاوی آفلاتوکسین B1 پس از گذشت 72 ساعت انکوباسیون، به طور معنی‌داری سبب افزایش قابلیت هضم ماده خشک نسبت به تیمار شاهد شد. فرآسنجه‌های تخمیری شکمبه از جمله نرخ و مقدار تولید گاز ، میزان pH مایع شکمبه و همچنین پارامترهای تخمینی از تولید گاز نیز در تیمارهای حاوی جاذب به طور معنی‌داری بهبود یافت. در رابطه با افزایش داده‌های حاصل از قابلیت هضم، فرآسنجه های تولید گاز، افزایش pH و پارامترهای برآورد شده از تولید گاز استفاده از بنتونیت نسبت به سایر جاذب ها مؤثرتر واقع شد. در خصوص تغییرات نیتروژن آمونیاکی افزودن جاذب‌های مختلف اثر کاهنده‌ای نسبت به تیمار شاهد داشت. به طور کلی می‌توان نتیجه گرفت که در مقایسه بین جاذب‌های مختلف، بنتونیت بیشترین تأثیر را در بهبود قابلیت هضم و متغیر‌های تخمیری شکمبه در شرایط برون‌تنی داشت.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Organic and Inorganic Aflatoxin B1 Absorbents on in Vitro Digestibility and Rumen Fermentation Characteristics

نویسندگان [English]

  • saeedeh assadzadeh heravi 1
  • Abdolmansour Tahmasebi 2
  • Abbas Ali Naserian 1
  • Reza Valizadeh 1
1 Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
2 Dept. of Animal Science, College of Agri. Ferdowsi University of Mahhad, Mashhad, Iran
چکیده [English]

Introduction Aflatoxins (AF) as secondary metabolites are produced by Aspergillus flavus and Aspergillus parasiticus. The most abundant aflatoxin B1 (AFB1) is toxic and carcinogenic to humans and animals. Utilization of mycotoxin adsorbents are noted as the most practical methods for protection of feed ingredients. The purpose of this study was to investigate the effect of organic and inorganic adsorbents for the adsorption of AFB1 and its effects on dry matter digestibility and rumen fermentation characteristics.
Materials and Methods The experimental diet was a mixture of alfalfa silage and concentrate. Procedure of in vitro batch culture was performed according to the Menke and Steingass procedure. In an anaerobic condition, 30 ml of buffered rumen fluid was dispensed with pipetor pump into a 120-ml serum bottle containing 0.5 g DM of the experimental diet. The content of each bottle was contaminated with 0.5 ppm AFB1. Experimental treatments were: the control diet, commercial bentonite, activated bentonite, activated charcoal, the cell wall of Saccharomyces serevisia with 75% purity, bentonite + activated charcoal + yeast, (0.5 + 0.4 + 0.1 percent), bentonite + activated charcoal + yeast, (0.4 + 0.45 + 0.15 percent) and , bentonite + activated charcoal + yeast(0.3 + 0.5 + 0.2 percent) The amount of absorbents for all treatments was 1% of the experimental diets. All bottles were purged with anaerobic CO2, sealed with rubber stoppers and placed in a shaking water bath for 72 h at 38.6 degree centigrade. The amount of produced gas was recorded at 2, 4, 6, 8, 12, 16, 24, 48 and 72 h of the incubation. At the end of incubation, all the bottles were transferred to refrigerator to stop fermentation, and then opened. After pH measurements 2-ml sample of each filtrate bottle was taken and frozen at 20 degree centigrade after acidification with 2-ml of 0.2 N HCl. The biomass residues were centrifuged at 1000×g for 10 min at 4 degree centigrade. The supernatant in each bottle was decanted and the pellet was dried at 65 degree centigrade to a constant weight for the determination of the residues.
Results and Discussion Absorbents addition increased the amount of produced gas in all treatments in comparison with the control treatment significantly (P

کلیدواژه‌ها [English]

  • Activated carbon
  • Aflatoxin
  • bentonite
  • In vitro
  • Yeast
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