Evaluation of IFN-γ and TGFβ1 Genes Expression in Guinea Pigs Vaccinated with Foot-and-Mouth Disease Type O Inactivated Vaccine

Document Type : Genetics & breeding

Authors

reza pasandideh ¹

Mohammadreza Nassiry ²

Ali Asghar Aslaminejad ³

Mojtaba Tahmoorespur ³

S. Zibaei

¹ Department of Animal Genetics and Breeding, Ramin Agriculture and Natural Resources University of Khuzestan, Mollasani, Iran

² Department of Anim al Science, Faculty of Agriculture, Ferdowsi University of Mashhad,Mashhad, Iran

³ Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

10.22067/ijasr.v5i3.31533

Abstract

Foot-and-mouth disease (FMD) is a severely contagious viral disease in cloven-hooved animals that it causes considerable economic losses in livestock productivity. Vaccination is one of the most effective procedures for control of FMD. One of vaccines performances is stimulating expression of some immune system genes, which called cytokines. In this study, expression changes of IFN-γ and TGFβ1 genes were evaluated in vaccinated guinea pigs with FMD type O inactivated vaccine. Blood samples were collected from vaccinated and control (no vaccinated) guinea pigs in three distinct times. After blood sampling, RNA was extracted and converted to cDNA. For measuring IFN-γ and TGFβ1 genes expression, relative Real-time PCR procedure was used. The results showed that expression of IFN-γ and TGFβ1 genes in the second and third blood sampling were significantly increased in comparison to the first blood sampling. Because increasing of cytokines expression is an indicative of the immune system response, these genes can be used as indicators for testing effects of the recombinant vaccines.

Keywords

Foot-and-mouth disease (FMD)؛ Guinea pig؛ IFN-γ؛ TGFβ1

Real-time PCR

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