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فرهاد صمدیان محمد امیر کریمی ترشیزی زربخت انصاری پیراسرایی حسین واثقی فهیمه محمد نژاد وحید واحدی

چکیده

این آزمایش برای ارزیابی اثرات سطوح مختلف چهار اسانس گیاهی نعناع، لیمو، آویشن و زنیان بر عملکرد و فراسنجه های خونی جوجه های گوشتی انجام شد. بدین منظور 312 قطعه جوجه گوشتی نر سویه ی آرین در قالب طرح کاملاً تصادفی به 13 گروه مختلف با شش تکرار اختصاص یافت. جیره‌های آزمایشی بر پایه ذرت و سویا و اسیدهای آمینه سنتتیک تهیه شدند. بعد از دو روز عادت پذیری با جیره پایه، هر یک از اسانس‏ها در سه سطح 50، 100 و 150 پی پی ام مکمل خوراک پایه شدند. وزن کشی به صورت هفتگی انجام گرفت و ضریب تبدیل غذایی و مصرف خوراک محاسبه شد. در روز 42 قبل از کشتار خون‏گیری به عمل آمد و برخی از فراسنجه های خونی از قبیل پروتئین کل، کلسترول، تری‌گلیسرید، HDL-c و LDL-c توسط کیت های تجاری مورد سنجش قرار گرفت. بیان نسبی ژن های فتی اسید سنتاز و مالیک آنزیم کبدی نیز با استفاده از RT-PCR صورت گرفت. نتایج نشان داد که دوز بالای اسانس نعناع موجب کاهش افزایش وزن روزانه پرنده‏ها در دوره رشد گردید. مکمل نمودن اسانس های گیاهی در مقایسه با شاهد، تأثیر معنی داری بر بیان ژن فتی‏اسید‏سینتاز کبدی، و متابولیت های خونی مورد سنجش نداشته است، در حالی که بیان نسبی ژن مالیک آنزیم کبدی در گروهی از پرندگان که از یک جیره مکمل شده با اسانس نعناع در سطح 150 پی پی ام تغذیه کرده بودند در مقایسه با گروه شاهد افزایش معنی داری نشان داد. بنابراین اسانس نعناع در سطح 150 پی پی ام می تواند بر بیان ژن آنزیم مالیک تأثیر گذاشته و همچنین بر عملکرد پرنده‏ها تأثیر سویی داشته باشد.

جزئیات مقاله

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ارجاع به مقاله
صمدیانف., کریمی ترشیزیم. ا., انصاری پیراسراییز., واثقیح., محمد نژادف., & واحدیو. (2015). اثر سطوح مختلف اسانس نعناع، لیمو، آویشن و زنیان بر عملکرد، فراسنجه های خونی و بیان ژن‏های لیپوژنیک کبدی در جوجه های گوشتی. پژوهشهای علوم دامی ایران, 7(3), 329-339. https://doi.org/10.22067/ijasr.v7i3.30357
نوع مقاله
علمی پژوهشی- تغذیه طیور