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p. 520−529
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80% progressive motility, volume of 0.75 to 2 mL, sperm concentrations greater than 3 × 109 sperm/mL and sperm abnormalities of less than 10%, were pooled. Different levels of Ascorbic acid (0, 0.5, 1, 1.5 and 2 mg mL-1) were added in tris-egg yolk based diluent. After processing and freezing, the samples were stored in liquid nitrogen until the time of evaluation. After thawing of semen samples, Sperm motility characteristics were analyzed using computer-assisted sperm analysis. The sperm viability was determined by means of the nigrosin–eosin staining method. The hypo-osmotic swelling test (HOS-test) was used to evaluate functional sperm plasma membrane integrity after freeze-thawing. Sperm abnormalities were assessed using Hancock solution. Lipid peroxidation was measured with determining malondialdehyde and the seminal plasma antioxidant enzymes of glutathione peroxidase, superoxide dismutase and total antioxidant capacity using the RANDOX Laboratories kit. Statistical analyses were performed using SAS software (9.1.3) software using GLM procedures. The least square means were calculated to determine the differences between the experimental treatments for the post-thaw evaluation times.
Results and Discussion The results showed that the 1 and 1.5 mg/mL ascorbic acid significantly improved the total motility (TM), progressive motility (PM), curvilinear velocity (VCL) compared to the control group (P< 0.05). Average path velocity (VAP) and straight line velocity (VSL) in semen samples receiving 1.5 mg ml-1 ascorbic acid was increased compared to control group (P< 0.05). But the Linearity percentage (LIN) and sperm track straightness (STR) was not significant compared to the control group. Viability and integrity of the plasma membrane parameters were improved in groups receiving 1 and 1.5 mg/ml ascorbic acid compared to the control group. The percentage of sperm with abnormal morphology in 1.5 mg mL-1 Ascorbic acid significantly was decreased than the control group (P]]>
p. 530−540
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p. 415−427
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p. 428−440
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p. 455−467
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p. 468−478
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p. 489−501
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p. 502−510
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p. 441−454
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0.05). The best feed conversion ratio (FCR) was observed in 110 g/days of FR (p]]>
p. 479−488
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p. 511−519
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