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0.05). In interaction effect, using apple pulp with probiotic reduced the amount of triglyceride and albumin meanwhile the amount of blood cholesterol increased (P>0.05). Apple pulp contains considerable amount of essentials nutrients such as vitamins and minerals and secondary substances like antioxidants, crude fiber and pectin. Use of apple pulp in hens diets improved the digestive tract health and highly amount of these compositions transfer to birds body and improve their performance, egg quality and blood parameters. The high amount of dietary fiber in apple pulp may be the main reason of blood parameters improving. The fibrous diets not only caused the secretion of bile increase but also increased the amount of cholesterol excretion compared to normal condition. Probiotic created a good environment in digestive tract by decreasing the population of harmful microorganisms, increasing the number of gram positive microorganisms and reduced pH of digesta in gut which can have positive effects on performance of laying hens. In diets containing probiotic, only productive parameters improved, whereas egg quality and blood biochemical parameters were not affected, meanwhile incorporated probiotic in apple pulp treatments, effectively improved egg quality traits and blood biochemical parameters more than performance. Apple pulp contain some composition that their digestion have some difficulties for laying hens, so, incorporation of probiotics such as protexin in diets those containing highly amount of undesirable composition such as fiber and pectin, can have good effects on egg production, egg traits and can improve blood biochemical parameters and be useful for efficient use of apple pulp by hens
Conclusion It can be resulted that using 4% of apple pulp in laying hens not only had no adverse effects on performance, egg quality traits and blood biochemical parameters, but also could effectively improve most of them, in contrary with more fibrous feeds that increased the amount of feed intake in bird, using 4% apple pulp did not have significant effects on the amount of daily feed intake in laying hens. Using 0.005% of probiotic can improve the performance, egg traits and blood parameters in laying hens.]]>
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