Effects of replacing different levels of seaweed Sargassum ilicifolum on performance,blood and ruminal metabolites of Zel lambs
Alireza
Vali Kamal
Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
author
Taghi
Ghoorchi
Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
author
Mahdi
Farahpour
Institute of Forests and Pastures of the country
author
Ashoor Mohammad
Gharebash
Department of Animal Science, Faculty of Agriculture, Gonbad Kavos University, Gonbad Kavos, Iran
author
Rahmat
Sameai
Golestan Province
author
text
article
2014
per
The seaweeds like Sargassum ilicifolum arecan used for animal nutrition. The aim of this study was evaluated the nutritional value of Sargassum ilicifolum as a food supplement in the diet of sheep. In this experiment were five diets containing zero, 10, 20, 30 and 40 percent of the seaweed were used. A completely randomized design was used to this purpose. In this study, the chemical composition of seaweed(Sargassum ilicifolum), DMdegradability,concentration electrolytes parameter from blood,rumen and performance parameters were measured. Treatment effects on feed intake were not significant(p>0.05). Water consumption increased with increase in algae value in deit. In this investigation in situ degradability of dry matter of seaweed in the intervals 0, 4, 8, 16, 24, 48, 72 and 96 hours was studied, amount of part fast degradability (a) and part slow degradability (b) for Sargassum illicifolumwere respectively 42% and33.6%. The effective degradability in outflow rates 2%, 5% and 8% were 49.6%, 45.5% and 44.3%, respectively. Effective treatment wasnot significant on the concentration Na and Cl, but was significant on the concentration K (P
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
4
no.
2014
279
285
https://ijasr.um.ac.ir/article_34130_f56a7aad667cf6e89559f0bc5313ff0e.pdf
dx.doi.org/10.22067/ijasr.v6i4.45416
Morphological Characteristic, Chemical Composition and In Vitro Gas Production Fermentation Profiles of Alfalfa Hay at Early Bud Stage Harvested at Different Cuts and in the Afternoon and in the Morning
Hadi
Ghorbani Farmad
Ferdowsi University of Mashhad
author
Abbas Ali
Naserian
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
Reza
Valizadeh
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
Mojtaba
Yari
Department of Animal Science, Faculty of Agriculture, Malayer University, Malayer, Iran
author
text
article
2014
per
There are a little information about nutritive value of alfalfa harvested at different cuts and different cutting time (morning and afternoon) in semiarid climate condition of north east of Iran. Effects of different harvesting cuts and cutting time (6 A.M and 6 P.M) of alfalfa hay at early bud stage on chemical composition and in vitro gas production fermentation profiles were studied in this experiment. Alfalfa hay that harvested at spring cuts had more CP and Ash content, gas production, ME, NEl, SCFA (short chain fatty acid), and IVOMD (In vitro organic matter digestibility) and less NDF and ADF than summer cuts. Alfalfa that cuts in the morning had more CP content and less leaf content and leaf to stem ratio than alfalfa cuts in the afternoon. Cutting alfalfa in the morning and the afternoon had no effect on fermentation profiles of alfalfa hay. The results of this experiment show that alfalfa hay at early bud stage harvested in spring and in the afternoon had more nutritive value than alfalfa harvested in summer and in the morning.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
4
no.
2014
286
294
https://ijasr.um.ac.ir/article_34148_c3b5fc6423bc03a143500b0a3a081453.pdf
dx.doi.org/10.22067/ijasr.v6i4.45418
Determination of Climate Critical Periods in Management of Dairy Cows in Northeast of Iran Using Temperature Humidity Index (THI)
Hamid
Taghavi
Ferdowsi university of mashhad
author
Abbas Ali
Naserian
Ferdowsi university of Mashhad
author
Reza
Valizadeh
Ferdowsi University of Mashhad
author
text
article
2014
per
This study was carried out to investigate the possibility of prediction the occurrence of heat stress in dairy cows and determination of climate critical periods. The most common indexes for estimation the degree of thermal stress istemperature humidity index (THI). In this study Yousef’s THI index (1985)was used to analyze the yearly and monthly effects of temperature and humidity. Meteorological data were obtained from the Northeast Regional Climate Center of Iran.After theTHI was calculated, the variability of THI was analyzed using scatter charts and THI values were analyzed as two-factor factorial design. Statistically, the changes of THI had constant trend over the year. Effects of years were meaningful and THI monthly means had significant differences during the year. The highest values of THI were evaluated in July that THI values in many hours were higher than the threshold values of the occurrence of heat stress. Moreover, 186291 records of milk production from a large industrial dairy farm were analyzed. Data showed that milk production also had constant trend over the year but with opposite direction of THI. In other words, when the amount of THI increased and passed of the threshold values of the occurrence of heat stress, milk production reduced and reached to the lowest level in July.Generally THI index is effective for determination of the critical periods of climate and prediction the probability of heat stress in dairy cows, and it can be a perfect guidefordairy farmersto perform adequate preventive acts.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
4
no.
2014
295
303
https://ijasr.um.ac.ir/article_34183_371111d8ee06e302624f0adc91d6f709.pdf
dx.doi.org/10.22067/ijasr.v6i4.21296
Comparing proteins and carbohydrates molecular structures in different sorghum cultivars using fourier transform infrared spectroscopy (FTIR) and multivariate analyses
Hojat
Gholizadeh
Ferdowsi university of mashhad
author
Abasali
Naserian
Ferdowsi university of Mashhad
author
Reza
Valizadeh
Ferdowsi University of Mashhad
author
Abdolmansoor
Tahmasebi
Ferdowsi university of mashhad
author
Pkovang
Yue
University of Saskatchewan
author
text
article
2014
per
This study was carried out to determine the protein and carbohydrate molecular structure of sorghum cultivars using Fourier Transform Infrared Spectroscopy (FTIR) with multivariate molecular spectroscopy analyses. Sorghum cultivars included: 1- Kimia, 2- Sepideh, 3- M2 and 4- M8. Protein and carbohydrate molecular functional groups studied included: peak area and height amide I, amide II, α-helix, β-sheet, 860 (non-structure carbohydrate), 928 (non-structure carbohydrate), total carbohydrate (CHO) with three major component peaks in this region, cellulosic compounds and different ratio of molecular structure. FTIR results showed that there were significant differences between sorghum cultivars in terms of proteins and carbohydrates molecular structures. Kimia had the greatest peak area and height amide I, II, α-helix, β-sheet, total carbohydrate and cellulosic compounds. Sepideh, M2 and M8 had similar proteins and carbohydrates molecular structures. Differences in protein and carbohydrate molecular structures can influence the availability of proteins and carbohydrates in ruminant and monogastric. Further studies needed to understand the effect of variety on protein and carbohydrate structure of sorghum and the relationship between protein and carbohydrate structure of a feed with nutrient availability in ruminant and monogastric
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
4
no.
2014
304
311
https://ijasr.um.ac.ir/article_34217_2a18d020933b7cc6e700a687fbaf14cc.pdf
dx.doi.org/10.22067/ijasr.v6i4.22305
Determining of Degradation and Digestion Coefficients of Canola meal Using of In situ and Gas production Techniques
Younes
Tahmazi
Uromiyeh University
author
Akbar
Taghizadeh
Department of Animal Science, Faculty of Agriculture, Tabriz University, Iran.
author
Yousef
Mehmannavaz
Islamic Azad University, Maragheh
author
Mehdi
Moghaddam
Islamic Azad University, Tehran
author
text
article
2014
per
This study was carried out to the determination of nutritive value of canola meal using naylon bag and cumulative gas production techniques in Gizel sheep. Tow fistulated Gizel sheep with average BW 45±2 kg used in a complete randomized design. The cumulative gas production was measured at 2, 4, 6, 8, 12, 16, 24, 36 and 48 h and ruminal DM and CP disappearance were measured up to 96 h. Coefficients of soluble CP degradation of canola meal (A), canola meal treated with 0.5% urea (B) and canola meal treated with micro wave (C) were 4.74, 15.81 and 15%, and for fermentable portion were 31.05, 39.62 and 65.55%, respectively. The cumulative gas production of soluble and insoluble portions (a+b) were 252.13, 213.57 and 240.88 ml/g DM. Metabolizable protein of treatments A, B and C were 283.11, 329.33 and 284.39 g/kg DM, that were not significantly different. The relationship between dry matter and cumulative gas production values for treatments obtained about 0.958, 0.976 and 0.932 and this parameter for crude protein and cumulative gas production achieved 0.987, 0.994 and 0.989, respectively. High correlation between in situ and cumulative gas production techniques indicated that digestibility values can be predicted from cumulative gas production data.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
4
no.
2014
312
323
https://ijasr.um.ac.ir/article_34246_b1d95c503f60ed6d8927f590dedc3407.pdf
dx.doi.org/10.22067/ijasr.v6i4.23501
The effect of dietary protein and lysine on egg quality and production of laying hens during 28-42 weeks of age
Hasan
Mohammadi Emarat
Department of Animal Science, Zabol Faculty of Agriculture, Zabol, Iran
author
Abolghasem
Golian
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
Abdolmansour
Tahmasebi
Dept. of Animal Science, College of Agri. Ferdowsi University of Mahhad, Mashhad, Iran
author
Hasan
Kermanshahi
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
text
article
2014
per
An experiment was conducted to evaluate the effect of dietary crude protein and lysine levels on quality and quantity of egg production. Fifteen diets consisted of 3 levels of protein (14, 15 and 16%) and 5 levels of lysine (0.71, 0.74, 0.77, 0.80 and 0.83 %) in a 35 factorial arrangement were provided. Each diet was randomly fed to 4 replicates of 12 birds, during four periods of 4 weeks (28-44wks of age). Egg number and mortality was recorded daily, whereas feed consumption determined for each period. Eggs from each replicate were weighed at the end of three consecutive days of each period and six eggs were used to measure the egg quality characteristics. Although the feed intake did not affected by dietary protein but the egg production, egg mass and feed conversion were improved significantly (p
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
4
no.
2014
333
341
https://ijasr.um.ac.ir/article_34232_9013c7eee31ea3ba51ba42bee5b5d136.pdf
dx.doi.org/10.22067/ijasr.v6i4.45421
Effect of Chavir (Ferulagoangulata) plant powder on liver and blood parameters of Japanese quail intoxicated with CCL4
Firooz
Samadi
Gorgan University of Agricultural Sciences and Natural Resources
author
abdolhosein
Pourkhanjar
Gorgan University of Agricultural Sciences and Natural Resources
author
Farzaneh
Ganji
Golestan University
author
Saba
Samadi
Gorgan University of Agricultural Sciences and Natural Resources
author
text
article
2014
per
This study aimed to evaluate the antioxidant property of Chavir plant on Japanese quail intoxicated with carbon tetrachloride (CCl4). Eighty, one-d-old Japanese quail chicks were randomly allotted to 4 treatments with 4 replicates and 5 chicks in each, using completely randomized design with 2×2 factorial arrangements including 2 levels of Chavir powder (0 and 1.5 percent) and CCl4 (0 and 1 ml/kg BW). The experimental period lasted 42 d and during this period feed and water were provided ad libitum. At the end of trial, birds (20 for each treatment) were bled through wing vein to determine blood parameters and hepatic enzymes. In addition, liver samples were taken for histology studies. Feed intake and body weight gain at different stages of experiment and feed conversion ratio throughout the experiment influenced by experimental treatments (P
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
4
no.
2014
342
350
https://ijasr.um.ac.ir/article_34276_375d08d4535ab60fc2213e041a896c6a.pdf
dx.doi.org/10.22067/ijasr.v6i4.45422
Study of polymorphism of Myostatin gene and its association with growth traits in lori sheep
Fatemeh
Bahramzadeh
Department of Animal Sciences, Faculty of Agriculture, University of Zabol, Zabol, Iran
author
Gholam Reza
Dashab
Department of Animal Sciences, Faculty of Agriculture, University of Zabol, Zabol, Iran
author
Masoud
alipanah
Department of Animal Sciences, Faculty of Agriculture, Zabol University, Zabol, Iran
author
Mohammad
Rokouei
Department of Animal Sciences, Faculty of Agriculture, Zabol University, Zabol, Iran
author
Sadegh
Asadollaei
Jahad Keshavarzi Lorestan province
author
text
article
2014
per
Myostatin gene or growth and differentiation factor-8 inhibits the puberty and development of skeletal muscles and it could change the size of some muscles such as double muscle, if the mutation occurs. One of the most important indigenous sheep in Iran, Lori sheep, possess high genetic potential for meat production. Therefore, the study of some genes affecting meat production would be of utmost important in selection programs. A total of 50 Lori sheep have been randomly selected and blood sampling was done to investigate the polymorphism of Myostatin gene and its relation to some economical traits including birth weight, weaning weight and 6-month lambs weight. Genomic DNA was extracted by the Denazyst kit. DNA quantity and quality was estimated by using espectophotometry and agarose electrophoresis. The specific primers were designed to assess the presence of potential polymorphism and then target area was amplified. After confirmation of 373bp fragment from exon 3 of Myostatin gene on the agarose gel, the genotype determination was performed using restriction enzyme HaeІІІ. The results showed that the Mm and mm genotypes were detected in the samples. The frequency of M and m alleles was 8.75 % and 91.25 %, respectively. No significant relationship was observed between genotypes of Myostatin gene and growth traits, except of the birth weight. In conclusion, the Myostatin locus could not considered as a promising marker for growth traits in Lori sheep.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
4
no.
2014
374
380
https://ijasr.um.ac.ir/article_34165_d8854e57372a2c6866a09e2d160a016c.pdf
dx.doi.org/10.22067/ijasr.v6i4.26789
Polymorphism in exon2 of BMP15 gene in Iranian sangsari sheep
zana
pirkhezranian
Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
Arezoo
Mohammadhashemi
Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
Mojtaba
Tahmoorespur
Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
Nasrollah
Pirany
Department of Animal Science, Faculty of Agriculture, Shahrekord University, Iran.
author
text
article
2014
per
Fertility rate is an economically important trait in sheep, which is influenced by genetic and environment. So far, three genes have been identified that affects this trait, one of them would be the BMP family, the most famous one is BMP15. Different mutations in the BMP15 gene, increases reproductive performance and growth rate in sheep. The aim of this study was to investigate the genetic and phylogenetic of BMP15 gene sequence in Iranian Sangsari sheep. For this purpose, the blood samples from 20 animal of Damghan station were collected. After DNA extracting, a segment of 222 bp of exon 2 of BMP15 gene was amplified using polymerase chain reaction. Then, all of the PCR products were sequenced. The results showed existence of four haplotypes and three significant mutations of the gene that which one of them was seen for first. In order to determine the genetic distance of Sansari sheep with other animals especially sheep breeds about 103 sequences were taken from Genebank, Then, phylogenetic trees were drawn. Genetic distances and nucleotide differences were calculated. The results showed that goat, cattle and buffalo have minimum genetic distance and monkey, human and mouse have maximum distance with Sangsari sheep and native Hindi and Kashmiri sheep have not any differences with Iranian Sangsari sheep.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
4
no.
2014
381
387
https://ijasr.um.ac.ir/article_34205_bfac03e6a6c32c5a71fedf33be781ba2.pdf
dx.doi.org/10.22067/ijasr.v6i4.27504
Polymorphisms of POU1F1 and STAT5A genes and their associate on with milk production traits in cattle
Sonia
Zakizadeh
Dept. of Animal Science, Higher Applied Science-Technology Institute
author
Ramin
Haeri
M.Sc. Graduated, Dept. of Animal Science, Islamic Azad University, Kashmar Branch
author
Akbar
Soleimani
Islamic Azad University, Kashmar Branch
author
text
article
2014
per
Specific trait candidate genes are sequenced genes with known biological activity. The effects of POU1F1 and STAT5A on milk production traits have been studied in several studies. POU1F1 affects on transcription of prolactin and growth hormone gene, as well as, STAT5A is known as a main mediator of growth hormone action on target genes and intracellular mediator of prolactin signaling. Since these genes are essential for development of mammary system, the aim of this study was to determine association of their polymorphism with milk production breeding values in Brown Swiss cattle. Blood of ninety milking cow were randomly obtained. DNA was extracted from whole blood using modified salting out method, then the desired fragments were PCR amplified and digested by specific restriction endonuclease enzymes. Gene and genotype frequencies, heterozygosity indexes, the real and effective allele number were calculated by PopGene software; and the breeding values of production traits were estimated by DFREML. SAS software was used to analyze association between genotypes and breeding values. The frequency of 'A' and 'C' alleles of POU1F1 and STAT5A were 0.455 and 0.489, respectively. This population was in hardy-weinburg equilibrium for both loci. There was no significant association between genotypes and breeding values, although POU1F1*B tended to produce higher milk and POU1F1*A showed higher fat and protein percent.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
4
no.
2014
388
394
https://ijasr.um.ac.ir/article_34261_873b61f7232ad9b5302f160f672014d7.pdf
dx.doi.org/10.22067/ijasr.v6i4.28771
Effect of garlic powder in the diet of sheep on digestibility, rumen fermentation and protozoa population
Mohammadhosein
Taherinia
Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Khuzestan, Iran.
author
Morteza
Chaji
Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Khuzestan, Iran.
author
Tahereh
Mohammadabadi
Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Khuzestan, Iran.
author
Moosa
Eslami
Department of Animal Sciences, Ramin University of Agriculture and Natural Resources, Khuzestan
author
Mohsen
Sari
Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Khuzestan, Iran.
author
text
article
2014
per
This experiment was conducted to investigate the effects of garlic powder on fermentation characteristic and rumen microbes of Arabian sheep.At first step the best level of garlic was determined. Then Animals were fed for 35 days with control diet and supplemented with garlic (2% per kg DM). Apparent digestibility, rumen fermentation parameters, number of protozoa and blood metabolites (glucose, urea and cholesterol) was determined. The results of the first phase, to determine the optimal level showed that dry matter digestibility were not affected by the presence of garlic in the diet, but numerically it was the highest in the diets containing 2% garlic powder (P>0.05). Also, NDF digestibility was the highest amount in this diet (P
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
4
no.
2014
324
332
https://ijasr.um.ac.ir/article_34287_425ef53993eb0fe2f0ba5669d95020c1.pdf
dx.doi.org/10.22067/ijasr.v6i4.28721
Effects of 1, 25-Dihydroxycholecalciferol and fruit hydroalcoholic extract of Withania Coagulans on bone histological properties and blood parameters of male chickens
Seyed Javad
Hosseini
Department of Animal Science, Faculty of agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
Hassan
Kermanshahi
Department of Animal Science, Faculty of agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
Hasan
Nasiri Moghadam
Department of Animal Science, Faculty of agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
Abolghasem
Nabipour
Department of Histology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
author
Ahmad
Hassanabadi
Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
author
text
article
2014
per
An experiment was conducted to investigate the effects of fruit hydroalcoholic extract of Withania coagulans (WC) and 1, 25-dihydroxycholecalciferol on bone mechanical and histological properties of male chickens. Diets were arranged factorially (2×3×2) consisted of a positive control with adequate Ca (10.4 g/kg diet) and nonphytate P (NPP; 5 g/kg diet) exceed the requirements suggested in the Ross 308 broiler nutrient specifications and a negative control diet with Ca (7.3 g/kg diet) and nonphytate P (NPP; 5 g/kg diet), three levels of Withania coagulans (0, 100 and 200 mg/kg diet), and two levels of 1, 25-dihydroxycholecalciferol (0 and 0.5 μg/kg diet). Both Ca and P retention significantly increased by reducing dietary Ca level (P
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
4
no.
2014
351
358
https://ijasr.um.ac.ir/article_34316_39f03223c53f77eb82c686181e174b81.pdf
dx.doi.org/10.22067/ijasr.v6i4.21056
The Effect of Physical Form of Feed on Performance and Nutritional Behaviors in Broilers
Mina
Toroghian
Department of animal Science, Kashmar Branch,Islamic Azad University, Kashmar, Iran.
author
Reza
Vakili
Department of animal Science, Kashmar Branch,Islamic Azad University, Kashmar, Iran.
author
text
article
2014
per
This experiment was conducted to evaluate the effect of physical feed form on performance and nutritional behaviors in broilers. This experiment was done in a completely randomized design with several observational with 3 treatments and 5 replicate. Dietary treatments included off of pellet form, crumble and mash form with similar components for 3 periods starter (0-10 days), grower (11-24 days) and finisher (25-42 days) were adjusted based on the requirements of Ross 308.The behaviors of eating, drinking, resting, walking. Chicks’ behavior marked in periods recorded. At the end of each period , chicks were weighted and feed intake and daily gain were measured to evaluate of performance. the best feed conversion ratio in the grower and finisher and whole of period was with crumble and pellet diet. increased Mash diet with significant effect eating and walking behavior and crumble resting behavior. Feed conversion ratio was positively correlated with eating , walking and with drinking and resting has negative correlation. According to the results of this experiment, with crumble and pellet form resting behavior increased which have most correlation with feed conversion ratio and it improved feed conversion ratio.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
4
no.
2014
359
364
https://ijasr.um.ac.ir/article_34329_4ee3725e3befac8b48629596f646b2c6.pdf
dx.doi.org/10.22067/ijasr.v6i4.26851
Study of mutations available in first-halfexon 2 of GDF9 gene in crossbred sheep born from crossing of Romanov rams with Kermani ewes
Rasoul
Khodabakhshzadeh
Shahid Bahonar University of Kerman
author
Mohammadreza
Mohammad Abadi
Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
author
Ali
Esmaili zadeh kashkuei
Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
author
Hossein
Moradi Shahrebabak
Department of Animal Science, Faculty of Animal and Agricultural Sciences Engineering, Faculty of Agriculture and Natural Resources, Karaj, University of Tehran
author
Sepideh
Ansari Namin
Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
author
text
article
2014
per
For decreasing the numberofbreedingewes onpastures and prevention of demolition pastures, Animal breedingprograms are necessary on genes with major effects on litter size in Iranian sheep breeds for Identify effective candidate genes on these economical traits .The GDF9 gene is one of the most important effective factors on litter size in sheep. The aim of the present study was to identify G2, G3 and G4 mutations in exon 2 of GDF9 gene in crossbred sheep (Romanov rams×Kermani ewes) using PCR-SSCP. Blood samples were collected from jugular vein of 121 crossbred individuals. Genomic DNA was extracted using salting-out method. Partial region of exon 2 (633 bp segments) of GDF9 gene was amplified with designed specific primers. The single stranded conformation polymorphism (SSCP) patterns of PCR products were studied using acrylamide gel electrophoresis and silver-nitrate staining method. Finally, we obtained 5 banding patterns 1, 2, 3, 4 and 5 with frequencies of 0.314, 0.024, 0.289, 0.232 & 0.141, respectively. The sequencing results showed presence 5 mutations in the studied population.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
4
no.
2014
395
403
https://ijasr.um.ac.ir/article_34302_ac0e34f82be9d86c4c86757728b54dcd.pdf
dx.doi.org/10.22067/ijasr.v6i4.37717
Development and evaluation of semi-purified diets for fiber related studies in Japanese quails
Vajihe
emampour
Department of Poultry Science, Faculty of Agricultural, Tarbiat Modares University,
Tehran, Iran
author
Mohammad Amir
Karimi Torshizi
Department of Poultry Science, Faculty of Agricultural, Tarbiat Modares University,
Tehran, Iran
author
Farid
Shariatmadari
Department of Poultry Science, Faculty of Agricultural, Tarbiat Modares University,
Tehran, Iran
author
text
article
2014
per
An experiment was carried out to develop and evaluate a semi-purified diet suitable for fiber related studies without negative impacts on performance, serum biochemistry and intestinal morphology of growing Japanese quails. Total of 144 Japanese quail chicks were used in a factorial arrangement using completely randomized design with 6 treatments, 4 replicates of 6 quails in each replicate. The levels of dietary crude fiber (3.37%, 1.18% and 0.08%, respectively) viz. high, medium and low crude fiber diets. The dietary supplementation levels of a commercial feed additive concentrate fiber-Arbocel were considered 0 and 3 %. The medium crude fiber semi-purified diet produced acceptable growth performances comparable to conventional high crude fiber diet. Serum triglyceride concentration was influenced by the levels of dietary crude fiber and the highest level was related to low crude fiber diet which was significantly different from high crude fiber diet (P
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
4
no.
2014
365
373
https://ijasr.um.ac.ir/article_34338_8182ac43044326a21057f96ea3ea3697.pdf
dx.doi.org/10.22067/ijasr.v6i4.26949