Effect of different levels of L-Glutamone and glycerol on coated ram spermatozoa in freezing procedure
Sanaz
Hasanpoor1
دانشگاه گیلان
author
Mohammad
Roostaei-Ali Mehr
دانشگاه گیلان
author
Mhrdad
Mohammadi
دانشگاه گیلان
author
text
article
2014
per
To determine the effect of different levels of L-glutamine and glycerol on freezing coated sperm, semen were collected from 4 Taleshi rams through artificial vagina contact with a tube containing Tris-fructose-egg yolk 15%. Ejaculates were pooled, centrifuged and supernatant was removed, then cell fractions were used. Samples were split into 9 equal parts and 3 (g3), 5 (g5) or 7% (g7) glycerol and 0 (G0), 80 (G80) or 160 (G160) mM L-Glutamine were added. Then samples were frozen by using liquid nitrogen and thawed after 2 weeks. Sperm motility, plasma membrane integrity, viability and acrosome reaction were investigated at 0, 3, 6 and 9 h after thawing. The results showed that there was an interaction between glycerol and L-glutamine on sperm motility and acrosome integrity. The highest sperm motility was observed in G80g5 (45±1.95), G0g7 (48.75±1.95) and G80g7 (50±1.95). The lowest sperm motility was achieved by G0g3 (26.5±1.95). The lowest acrosome integrity was observed in G160g3 (76±0.94). The highest plasma membrane integrity (45.28±1.57) and sperm viability (36.71±1.16) was observed in 80 mM L-glutamine. The highest sperm viability was observed in 7% glycerol (41.71±1.58). Therefore, 7% of glycerol and 80 mM of L-glutamine could be beneficial to freeze coated ram spermatozoa.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
1
no.
2014
https://ijasr.um.ac.ir/article_33306_b9e1cc2b93964aba8d616a2dd191ebbd.pdf
dx.doi.org/10.22067/ijasr.v6i1.21872
Effect of Milking Frequency in Early Lactation on Milk Production, some Blood Metabolites and Reproductive Performance of Holstein Dairy Cow
Abolfazl
Kiani
Department of Animal Science, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran.
author
Ahmad
Riasi
Department of Animal Science, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran.
author
Saeed
Ansari Mahyari
Department of Animal Science, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran.
author
Gholamreza
Ghorbanei kharage
Department of Animal Science, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran.
author
Mohammad
Khorvash
Department of Animal Science, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran.
author
text
article
2014
per
The objective of this study was to evaluate the effect of different milking frequencies in the first 6 weeks of lactation on milk production and milk constituents, blood metabolic profiles and reproductive performance of fresh dairy cows. The milking frequencies imposed were three times daily milking for 42 days (3X), six times daily milking for the first 21 days of lactation and three times daily subsequently (6X-3X) and six times daily milking for 42 days. For this purpose 21 multiparous Holstein dairy cows were allocated to three groups based on BCS, parity, and body weight. Results showed that the mean of milk and FCM production was significantly higher for 6X than 3X cows in first and second 21 days and in the entire period. Among milk constituent only fat production was affected by milking frequencies. The milking frequency had no effect on mean DMI. Weight loss of the cows was higher for 6X cows (-32 kg) than those the 6X-3X (-29 kg) and 3X (-29.1 kg). Blood concentration of NEFA was affected by milking frequencies and it was significantly higher for 6X compared to 3X. The mean concentration of blood progesterone and reproductive parameters was not affected by milking frequencies. It was concluded that 6 time milking per day in a short term period may inrease Holstein dairy cows’ performance without any adverse effect on their reproductive parameters.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
1
no.
2014
https://ijasr.um.ac.ir/article_33324_720fec9f9464c0f215d4af481e1109cb.pdf
dx.doi.org/10.22067/ijasr.v6i1.37613
Growth Nutritive Value of Saffron Residues Harvested at Different Stages by in situ and in vitro (Gas Production) Methods
Vahid
Kardan Moghadam
Department of Animal Sciences, Faculty of Agriculture, Birjand University, Birjand, Iran
author
Mohammad Hassan
Fathi Nasri
Department of Animal Sciences, Faculty of Agriculture, Birjand University, Birjand, Iran
author
Reza
Valizadeh
Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
Seyyed Homayoun
Farhangfar
Department of Animal Sciences, Faculty of Agriculture, Birjand University, Birjand, Iran
author
text
article
2014
per
The chemical composition, ruminal degradability parameters, ruminal and post ruminal digestibility of saffron residues were determined using in situ and in vitro (gas production) methods. The harvested residues at late vegetative phase were compared with the residues harvested at the early dormant phase. The results showed that NDF and ADF concentration of harvested residues at early dormant phase were higher where as it content of CP was significantly (P
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
1
no.
2014
https://ijasr.um.ac.ir/article_33357_c50f2729d016cb55691381fb2a490d1d.pdf
dx.doi.org/10.22067/ijasr.v6i1.37615
Genetic Evaluation of Test Day Milk Records of Holstein Cows in the Northern Provinces of Iran Using Random Regression Models
mid
Hasanzadeh
Animal Science Department, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
author
Seyed Hasan
hafezian
Animal Science Department, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
author
Homayon
Farhangfar
Department of Animal Science, Birjand Faculty of Agriculture, Birjand, Iran
author
text
article
2014
per
This research used the test day data of the milk production of Holstein cows from Golestan, Mazandaran and Guilan provinces collected by Animal Breeding Center of Iran between the years of 1988-2011. Permanent effects of model, including cattle blend, breeding year and season, also percent of Holstein gene and age during recording, were used as a secondary variable. All data obtained in this research was 92943 test day records of milk production from 12855 Holstein heifers. DFREML was applied to estimate variance components and genetic parameters in test day models (with random regression restricted maximum likelihood method). A third-order Legendre polynomial has been used in order to considering genetic and permanent environmental variations of milk production for cows lactation period. Analyzing the environmental factors made by SAS software program. Maximum estimation of genetic, phenotypic and permanent environmental correlations of milk yield was 0.99, 0.61 and 0.98, respectively The results of this research shows that the heritability of milk production are different from each other in the different months of lactation period and was achieved in the range of 0.08- 0.19, respectively.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
1
no.
2014
https://ijasr.um.ac.ir/article_33386_998b8bbcb319850f0d3f054df1129178.pdf
dx.doi.org/10.22067/ijasr.v6i1.37616
Determining Nutritive Value of Alfalfa hay and Wheat straw from East Azerbaijan province
hossein
janmohammadi
تبریز
author
Akbar
Taghizadeh
Department of Animal Science, Faculty of Agriculture, Tabriz University, Iran.
author
Parviz
Yasan
Tabriz
author
Jalil
Shojaa
Tabriz
author
Ali
Nikkhah
Department of Animal Science, Faculty of Agriculture, Science and
Research Branch, Islamic Azad University, Tehran, Iran
author
text
article
2014
per
This study was conducted to determine nutritive value of common animal feed sources in East Azerbaijan province including second cutting of alfalfa hay ( N=35) and wheat straw (N=26). Chemical composition and mineral contents of alfalfa hay and wheat straw were determined according to standard methods. Then the average chemical compositions of these samples were compared with those of the Gilan, Kurdistan and Kermanshah samples and the National Research Council (NRC) data. The results demonstrated that the mean dry matter (DM) content of alfalfa hay and wheat straw were 942.4± 5.6 and 948.7 ± 6.5 g/kg, and mean crude protein (CP) concentrations were 158.6 ± 11.4 and 40.7 ± 4.8 g/kg of dry matter, respectively, that showed a significant difference with NRC data. Neutral Detergent Fiber (NDF) content of alfalfa hay was 347.5 ± 51.5 g/kg and Acid Detergent Fiber (ADF) of alfalfa hay and wheat straw were 447.3 ± 39.2 and 703.1 ± 5.55 g/kg, respectively; that showed no significant difference. The content of calcium in the alfalfa hay was 13.9 ± 3.5 g/kg that revealed no significant difference with the NRC data. The calcium concentration of wheat straw was 5.7 ± 1.4 g/kg, that differed significantly with the NRC data. The gross energy of alfalfa hay and wheat straw were 4100±154 and 3981± 126 kcal/ kg, respectively. In conclusion, it is recommended that agricultural managers in regional programming, farmers and feed manufacturers in balanced feed formulation use this data.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
1
no.
2014
https://ijasr.um.ac.ir/article_33408_ba6714a84880c52f97820b10477b58a7.pdf
dx.doi.org/10.22067/ijasr.v6i1.20857
The investigation of Leptin gene Promoter in the Najdi Cows
fatemeh
amraei
کشاورزی و منابع طبیعی رامین
author
Hedayatollah
roshanfekr
Department of Animal Science, University of Khuzestan Agricultural Sciences and Natural Resources, Khuzestan, Iran.
author
Jamal
Fayazi
کشاورزی و منابع طبیعی رامین
author
Mohammad
Bojarpour
Department of Animal Sciences, Faculty of Animal Sciences and Food Industry, University Agriculture and Natural Resources Ramin Khuzestan, Khuzestan, Iran.
author
text
article
2014
per
Identification of associated genes with energy balance, yield and feed intake are recent interests of the animal breeding researchers. Najdi breed is the famous cattle breed in the Khuzestan province. In this research for The investigation of Leptin gene Promoter ,from 15 Najdi cows in the Shushtar cattle center station was taken. DNA from whole blood was Extracted and 544bp and 566 bp two pieces of was used for amplification. After ensuring the correctness PCR products on agarose gel 1% sequences gene was performed and component promoter leptin was found in Najdi cow. In the first fragment(566bp) of the study was recognized, 6 Mutation, two point mutations one of the transition and the other was transversion and four frameshift mutations that mutations of another typ of, deletion and insertion. In the second segment(544bp( was observed in the leptin gene promoter region two point mutations one of the transition and the other was transversion and deletion of a frameshift Mutation The results of this study showed that reports Mutations on boxes and component promoter did not affect leptin gene.In this study was compared the sequence corresponding to a single on sample in terms of both pieces were registered on sequence similarity with other region that blast results showed a high percentage of similarity.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
1
no.
2014
https://ijasr.um.ac.ir/article_33435_8c4eeb6812cbefb5ef4b6d52529ca80f.pdf
dx.doi.org/10.22067/ijasr.v6i1.22328
The Effect Alfalfa Leaf Meal on Performance, Egg Quality and Blood Parameters of Laying Hen
Ali
Nobakht
Department of Animal Science, Faculty of Agriculture, Islamic Azad University, Maragheh Branch, Maragheh, Iran
author
text
article
2014
per
This experiment was conducted to evaluate the effect of increasing levels of alfalfa leaf meal (ALM) on egg production, egg traits and blood parameters of laying hens with 144 Hy-line (W36) laying hens from 65-75 weeks of age in 4 treatments, 3 replicates and 12 hens in each replicate in a completely randomized design. Experimental groups included: 1) control group, 2) group with 1% of ALM, 3) group with 2% of ALM, 4) group with 3% of ALM. Diets with 2% and 3% increased egg production, reduced the amount of feed intake, improved the feed conversion ratio and reduced the egg production cost (P
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
1
no.
2014
https://ijasr.um.ac.ir/article_33453_201ce5541b602930a62d800a0e53bec6.pdf
dx.doi.org/10.22067/ijasr.v6i1.37608
Investigating the effects of Salvia mirzayanii essential oil on rumen microbial fermentation and nutrient digestibility using gas production and dual flow continuous culture system
Younes
Ouladshanbe
Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Khuzestan, Iran.
author
Mohsen
Sari
Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Khuzestan, Iran.
author
Morteza
Chaji
Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Khuzestan, Iran.
author
Tahereh
Mohammadabadi
Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Khuzestan, Iran.
author
Mohammad
Bojarpour
Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Khuzestan, Iran.
author
text
article
2014
per
This study was conducted to investigate the effects of different doses of salvia mirzayanii essential oils on gas production parameters, fermentation characteristics using gas production and dual flow continuous culture system. Trearments in the gas production experiment was adding 0, 400, 800, 1200, 1600, 2000, 2400 and 4800 mg/l of salvia mirzayanii essential oils to the media. In the second experiment, five 1750-mL dual-flow continuous culture fermenters were used in 3 replicated periods of 9 d. Temperature (38.5°C) and liquid and (10%/h) solid (5%/h) dilution rates were kept constant in the fermenters. Fermenters were fed daily with 120 g dry matter. Treatments arranged as complete randomized block design included levels of essential oils from salvia mirzayanii (800, 1600, 2400 mg/l) and a monensin (0.1 percent of the diet dry matter). Addition essential oils of salvia mirzayanii to diet lead to decrease asymptotic gas production, organic matter degradability and biomass. Dry matter digestibility and NDF with the level of 800 mg/l essential oil compered to control treatment were significantly increased. Concentration of NH3-N was less for pre-feeding (0 h) in treatments with T2 and T3 compared with control treatment. The average of concentration NH3-N was lowest in highest level of essential oils eight hours after feeding. The results showed that using of low level of salvia mirzayanii essential oils could improve nutrient digestibility in the rumen.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
1
no.
2014
https://ijasr.um.ac.ir/article_33473_81130a2c4267508ed5ff4a7a504b908e.pdf
dx.doi.org/10.22067/ijasr.v6i1.21016
Comparison of single nucleotide polymorphisms of Mx gene in different strains of commercial broiler, layer and native chickens
sedighe
malekshahdehi
دانشگاه کشاورزی ساری
author
Seyed Hasan
Hafezian
دانشگاه علوم کشاورزی و منابع طبیعی ساری
author
Ghodratollah
Rahimimianji
Department of Animal Sciences, Faculty of Animal Sciences and Fisheries, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran.
author
Zarbakht
Ansari Pirsaraei
Department of Animal Sciences, Sari University of Agricultural Sciences and Natural Resources
author
text
article
2014
per
Avian influenza is a contagious viral disease affecting the respiratory, digestive and nervous system of birds belong to orthomyxovirus family. In the chicken Mx gene, type of amino acid at position 631 determines the antiviral activity against influenza and vesicular stomatitis virus. A single nucleotide polymorphism (SNP) at position 2032, responsible for the variation of amino acid at position 631 (Ser to Asn) of the Mx protein, has been demonstrated to be responsible for positive or negative antiviral activity of Mx proteins., The allele A and G at position 2032 corresponds to the positive and negative antiviral activity, respectively. In order to identify gene polymorphism at Mx locus, 250 blood samples randomly were collected from Sari, Shiraz, Mashhad native fowls breeding station , commercial broiler and layer flocks. DNA was extracted and a fragment with the length of 299 bp from the exon 13 of Mx locus was amplified by a specific primer pairs. PCR-RFLP method was used to identify of polymorphism in coding sequence of the Mx gene using Hpy8I restriction enzyme. The frequency of alleles were estimated A (0.692) and G (0.308) in Sari, A (0.577) and G (0.423) in Shiraz, A (0.604) and G (0.396) in Mashhad, A (0.46) and G (0.54) in commercial broiler and A (0.86) and G (0.14) in layer strain, repectively. According to the observed polymorphism at position 631 of Mx protein and its important role in susceptibility to influenza disease it may be possible to use this marker gene in breeding programs.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
1
no.
2014
https://ijasr.um.ac.ir/article_33496_acfcaf0757ab42825a702f2e83ff68d9.pdf
dx.doi.org/10.22067/ijasr.v6i1.26990
Nutritive Value of Three Soybean Meals through in vitro techniques and in vivo evaluation in Broiler Chickens
Marzieh
Afkhami
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
Hasan
Kermanshahi
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
Abolghasem
Golian
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
text
article
2014
per
A CRD experiment was conducted to study three different soybean meals on experimental qualitative properties, digestibility of protein and performance in broiler chickens for a 42 day period. A total of 195 day-old Ross 308 male broiler chickens were randomly distributed to 3 dietary treatments with five replicates of 13 chicks each. To measure digestibility of protein, thirty 15-day-old Ross 308 male broiler chickens were randomly distributed to 3 dietary treatments with five replicates of 2 chicks each. Three soybean meals including two Iranian and one Argentinean ones classified according to experimental properties (Urease activity, Protein Solubility and Heat Damaged Index) into classes of high, medium and low quality soybean meals were used. Protein digestibility was similar in different treatments. Weight gain and feed intake only in starter and feed conversion ratio in each period were not affected by treatments. Weight gain and feed intake in growing period increased significantly using high quality soybean meal, However they were not affected by low and medium quality soybean meals. Weight gain and feed intake were not affected by high and low quality soybean meals in finisher and overall and there was not also significant using medium and low quality soybean meals during the overall period. However they decreased by medium quality soybean meal in finisher and overall period. Pancreas and abdominal fat weights at 21d and 42d were not affected by different treatments. Liver weight at 21d was the same in different treatments while it decreased significantly at 42d by medium quality soybean meal.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
1
no.
2014
https://ijasr.um.ac.ir/article_33518_8d9773388e95d592b39033346636fc02.pdf
dx.doi.org/10.22067/ijasr.v6i1.37609
Effects of Chemical Inhibitors on Fermentation Pattern, Methanogenesis and Hydrogen Emission in Ruminal Batch Cultures
Marzie
Ghadami Koohestani
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
Reza
Valizadeh
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
Abbas Ali
Naserian
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
Seyed Hadi
Ebrahimi
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
text
article
2014
per
The objective of this meta-analysis was to compare effects of chemical methanogenic inhibitors on fermentation parameters in ruminal batch cultures. Nine published in vitro studies including 26 treatment means in which 2-Bromoethane sulfonate, Bromochloromethane and 9,10-Anthraquinone were used as methanogens inhibitor compared in amount of CH4, H2, total volatile fatty acid, actate, propionate and butyrate production. The results showed that all chemical inhibitors reduced methane production significantly (-2.64±0.290) and 2-Bromoethanesulfonate was the most effective inhibitor. The inhibitors had a significant negative effect on acetate production (-1.48±0.255), and positive effect on molar proportions of propionate (+0.94±0.309) and butyrate (+0.69±0.125). The reduction in acetate production was not compensated by increase in propionate and butyrate production, therefore total VFA production reduced (-0.42±0.180) significantly. The inhibitors increased H2 production (+2.31±0.540) significantly and it was less than the predicted amount by stoichiometry equation. It was concluded that although the applied methanogens inhibitors were successful in reducing methane emission, but less VFA recorded and energy lost in form of H2 was significant in batch culture systems.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
1
no.
2014
https://ijasr.um.ac.ir/article_33535_c5c2d44348805a79c20dd7d40e95c0a8.pdf
dx.doi.org/10.22067/ijasr.v6i1.27873
Effect of using different levels of Nigella sativa meal on the growth performance and meat quality of Japanese quails
Tayebeh
Mousapour
Department of Animal Science. College of Agriculture. Shahid Bahonar University of Kerman. Kerman. Iran
author
Mohammad
salarmoini
Department of Animal Science. College of Agriculture. Shahid Bahonar University of Kerman. Kerman. Iran
author
text
article
2014
per
This study was conducted to investigate the effects of dietary Nigella sativa meal (NSM) on the growth performance, blood parameters, carcass characteristics, egg yolk cholesterol and meat quality in Japanese quail. For this purpose 300 one-day old Japanese quail chicks in a completely randomized design was used with five treatments in four replicates )containing 15 birds each(. The experimental diets were control group (without NSM) and diets containing 5, 10, 15 and 20% of NSM. Feed intake of birds fed diets with 5 and 15% of NSM were statistically higher than control group. Weight gain in birds fed with 15% of NSM was significantly higher than control group. Feed conversion ratio of birds fed diets with 20% of NSM was statistically higher than control. No significant treatment effect was observed on egg yolk cholesterol, blood parameters (including triglyceride, HDL and total cholesterol concentrations) and meat quality parameters (TBA, WHC, Dripping loss, Cooking loss). And also the use of different levels of NSM had no any significant effect on internal organs weight, except gizzard. NSM significantly decreased feed costs compared to control group. So, by using NSM up to 15% in Japanese quails diets, can improve weight gain and feed conversion ratio and decrease feed and meat production costs.
Iranian Journal of Animal Science Research
Ferdowsi University of Mashhad
2008-3106
6
v.
1
no.
2014
https://ijasr.um.ac.ir/article_33552_526b13ca4dcbd6799dd8298a855bd65b.pdf
dx.doi.org/10.22067/ijasr.v6i1.23655