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وحید تقی زاده محمدرضا نصیری مجتبی طهمورث پور محمدرضا بختیاری زاده علی جوادمنش

چکیده

سندروم آسیت یک عارضه متابولیکی است که در سنین بالا در پرندگان گوشتی بروز می‌کند و به سویه تجاری آرین که تنها لاین اصلاح شده مرغ گوشتی ایران می باشد آسیب جدی وارد کرده است. مطالعات گذشته نقش عوامل ژنتیکی در بروز این سندروم را اثبات کرده است. از آنجا که ارگان‌های متعددی در بروز آسیت دخالت دارند و نقش قلب در آنها از همه بارزتر است، از مطالعات ژنومی استفاده شد تا ژن‌های مرتبط با مسیر آنژیوژنز در هایپرتروفی بطن راست قلب شناسایی شوند. در این تحقیق تعداد 464 پرنده از خط پدری B لاین آرین تحت تنش سرمایی پرورش داده شد و در بررسی آنالیز داده هایRNA-seq بین دو گروه سالم و مبتلا به آسیت، پروفایل بیان ژن‌ها در بطن راست قلب مرغان حساس و مقاوم به آسیت با استفاده از تکنولوژی توالی‌یابی نسل آینده مورد مقایسه قرار گرفت. نتایج بررسی ترنسکریپتومی ژنهای مسیر آنژیوژنز نشان داد که تعداد 59 ژن بین دو تیمار سالم و آسیتی تفاوت بیان معنی‌دار داشتند )05/0P<) که از این تعداد 29 ژن افزایش بیان و 30 ژن کاهش بیان نشان دادند. بر اساس نتایج هستی شناسی ژنی، بیشترین معنی داری در پروسه‌های بیولوژیکی به مسیرهای برهم‌کنش گیرنده¬های برون یاخته ایی (ECM-receptors)، تقسیم سلولی میتوز، پارتیشن بندی کروموزومی، چرخه سلولی و اینترلوکین¬ها تعلق داشتند. بر اساس نتایج آنالیز شبکه از ژن¬های افزایش بیان یافته در بیماری آسیت، سه خوشه ژنی تشکیل شد که ژن¬هایی از قبیل CDK1، COL6A2 و IL7R به‌عنوان مهم¬ترین ژن¬های مرتبط با آنژیوژنز معرفی شدند.

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ارجاع به مقاله
تقی زادهو., نصیریم., طهمورث پورم., بختیاری زادهم., & جوادمنشع. (۱۳۹۶-۰۵-۲۴). مطالعه شبکه ژنی آنژیوژنز در جوجه‌های مرغ آرین حساس به آسیت با استفاده از داده‌های RNA-Seq. پژوهشهای علوم دامی ایران, 10(2), 249-262. https://doi.org/10.22067/ijasr.v10i2.65215
نوع مقاله
علمی پژوهشی- ژنتیک و اصلاح دام و طیور

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