Study of Chemical Composition and in vitro Fermentation of Barley Roddasht Cultivar and Mutant Lines of Safflower and Cottonseed

Document Type : Ruminant Nutrition

Authors

1 Ferdowsi University of Mashhad

2 Zanjan

3 Tarbiat Modares

Abstract

Introduction By the end of the fourth development plan, annual grain shortages and protein meal for animal feed were 807 and 795 thousand tones, respectively. In the first nine months of 2014, an average of 1187 and 1865 thousand tons of protein meals were imported to the country. In recent years, due to the lack of water, salinity of water and soils, crop production has shown a significant decline. So, one of the suggestions in this regards is creation of new varieties with properties of higher production and resistant to environmental stresses. Fortunately, a lot of efforts in the country and especially in Nuclear Science and Technology Research Institute have been down for introducing different varieties of crop which is resistant against environmental stress. In most of these studies gamma ray has been used as a mutagenesis to create the lines and new varieties. Part of this research has led to introduce of the barley cultivar, Roddasht, and some lines of safflower, cottonseed and other crop species. Unfortunately, nutritional and digestive value of seed of these new cultivar and lines are not known for animals in line with their introduction. The aim of this study was to test the chemical composition, fermentation characteristic, digestible organic matter, metabolisable energy, VFA production and protein properties of barley (variety Roddasht) and mutant lines of safflower and cottonseed.
Materials and Methods Cultivar of barley (Roddasht) and mutant lines of cottonseed and safflower were grown at research field of Nuclear Agriculture Research School. These cultivar and mutant lines were achieved via mutation breeding process of their parent's cultivare (i.e. Valfajr (Barley), Varamin (Cotton) and Zarghan (Safflower), respectively). A 1 kg sample (seed) of each was used in this study. All samples were ground through 2 mm screen (Retsch Cutting Mill, Retschmule, Germany) and used for subsequent analyses. The samples were analyzed in duplicate for crude protein (CP; kjeltel auto 1300, Foss, Denmark), and organic matter (OM). Neutral detergent fiber (NDF) and acid detergent fiber were determined. Gas production (GP) test was performed. Approximately 200 mg of dry matter (DM) of each treatment was placed in 100 ml syringes (four replicates) and 30 ml of incubation medium was then added to them. Gas production (GP) test was performed for three consecutive weeks. Rumen fluid was collected from the three cannulated sheep and squeezed through four layers of cheese cloth. Gas production was measured at 2, 4, 6, 8, 12, 24, 48, 72 and 96 h and a set of appropriate blanks was included. Gas produced from each treatment was calculated by subtracting the produced gas form gas produced in a control blank. Metabolisable energy, digestible organic matter and volatile fatty acids were estimated via available equations. Protein of seeds was extracted using Tris-HCl method. Extracted proteins were run on SDS-Pages and the protein lanes were stained with coomassie blue dye.
Results and Discussion No significant differences were observed in chemical composition of mutant lines of cottonseed and safflower compared to their parental lines. Barley cultivar, Roddasht, had a lower (P

Keywords


1- AOAC International. 1998. Official Methods of Analysis. 14th ed. AOAC International, Gaithersburg, MD.
2- Colkesen, M., A. Kamalak., O. Canbolat., Y. Gurbuz, and C. O. Ozkan. 2005. Effect of cultivar and formaldehyde treatment of barley grain on rumen fermentation characteristics using in vitro gas production. South African Journal of Animal Science, 35(3): 206-212.
3- Dure, L. S. and C. Chlan, 1981. Developmental biochemistry of cottonseed embryogenesis and germination. XII-Purification and properties of principal storage proteins. Plant Physiology, 68(1):180-186.
4- Ministry of Agriculture. 2014. Export and import of agricultural products in first nine months of 2014. Planning and Economic Information and Communication Technology Center. (In Persian).
5- Getachew, G., H. P. S. Makkar, and K. Becker. 2002. Tropical browses: contents of phenolic compounds, in vitro gas production and stoichiometric relationship between short chain fatty acid and in vitro gas production. Journal of Agricultural Science, 139(3): 341-352.
6- Ghorbani, G. R. and A. Hadj-Hussaini. 2002. In situ degradability of Iranian barley grain cultivars. Small Ruminant Research, 44(3): 207-212.
7- Givens, D. I., P. Clark, D. Jacklin, A. R. Moss, and C. R. Savey. 1993. Nutritional aspects of cereal, cereal grain by products and cereal straw for ruminants", HGCA research Review No. 24, Home Grown Cereals Authority, Hamlyn house, Highgate Hill, London, UK. pp. 1-180.
8- Golkar, P., A. Arzani, and A. M. Rezaei. 2011. Genetic variation in safflower (Carthamus tinctorious L.) for seed quality-related traits and inter-simple sequence repeat (ISSR) markers. International Journal of Molecular Scienice, 12(4): 2664-2677.
9- Hajmansoor, S. , M. R. Bihamta., A. Nabipour., A. Mohammadi., S. M. Pirseyedi, and H. R. Nikkhah. 2013. Genetic diversity in barley genotypes: I. Seed storage proteins (hordeins) and agronomic traits. Seed and Plant Improvment Journal, 25(4): 585-604. (In Persian).
10- Heisel, S. E., D. M. Peterson and B. L. Jones. 1986. Identification of United States barley cultivars by sodium dodecyl sulfate polyacrylamide gel electrophoresis of hordeins. Cereal Chemistry, 63(6): 500-505.
11- Khezri, A., M. Yoosofi-Ansari, and M. R. Mohammd Abadi. 2011. Analysis the protein subunits and fractions of almond meal in comparison to soybean meal and cottonseed meal using SDS-Page electrophoresis and CNCPS method. Journal of Agricultural Biotechnlogy, 3(2): 15-25. (In Persian).
12- Laemmli, U. K. 1970. Cleavage of structure proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259): 680-685.
13- Menke, K. H., and H. Steingass. 1988. Estimation of the energetic feed value obtained from chemical analyses and gas production using rumen fluid. Animal Research and Development, 28(3): 7-55.
14- Miller F. R. and J. A. Stroup. 2003. Brown midrib forage sorgum, sudangrass and corn: what is the potential? 33rd California Alfalfa & Forage Symposium, Monterey, California.
15- National Research Council. 2012. Nutrient Requirements of Dairy Cattle, 7th rev. ed. National Academy Sciences, Washington, DC.
16- Obreht, D. R., L. B. Vapa., S. A. Kis., M. Takacs-Hajos., E. Stefanovics-Banyai., G. Vorosvari, and R. R. Kastori. 2002. Seed protein variability in safflower. Proceedings for Natural Sciences, Matica Srpska, Novi Sad, No.103: 29-34.
17- Porter, L. J., L. N. Hrstich, and B. G. Chan. 1986. The conversion of procyanidins and prodelphinidins to cyanidin and delphinidin. Phytochemistry, 25(1): 223-230.
18- Ranjan, S., R. Matcha, B. Madhuri, and N. Babu. 2012. Comparative evaluation of protein extraction methods from few leguminous seeds. International Journal of Advanced Biotechnology and Research, 3(2): 558-563.
19- Sammour, R. H., S. M. N. El-Shourbagy., A. M., Abo-Shady, and A. M. Abasary. 1995. Proteins of cottonseed (Gossypium barbadense): Extraction and characterization by electrophoresis. Qatar University Science Journal, 15(1): 77-82.
20- SAS Institute. 2008. SAS User’s Guide: Statistics, Version 9.0 Edition. SAS Inst. Inc., Cary, NC.
21- Shinvari, Z. K., H. Rahman, and M. Ashiq Rabbani. 2014. SDS-Page based genetic divergence in safflower (Carthamus Tinctorius L.). Pakistan Journal of Botany, 46(3): 811-815.
22- Ministry of Agriculture. 2004. Statistics of agricultural products. Department of Statistics and Information. (In Persian).
23- Van soest, P. J., J. B. Robertson, and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in ration to animal nutrition. Journal of Dairy Science, 74(10):3583-3597.
24- Yaghobfar, A., S. A. Ghafari, and A. Yousefi. 2012. Determination nutritive value of hull-less barley cultivars used in poultry nutrition. Animal Sciences Journal (Pajouhesh & Sazandegi), 97(4): 15-23. (In Persian).
25- Zahedifar, M., H. Fazaeli, and N. Teimournejad. 2014. Study of chemical composition and fermentability of straw from some varieties and lines of wheat. Journal of Ruminant research, 1(4):81-95. (In Persian).
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